Lab Chip, 2024, 24,2107-2121
DOI: 10.1039/D3LC01075A, Paper

Open Access

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Wannes Verbist, Jolien Breukers, Sapna Sharma, Iene Rutten, Hans Gerstmans, Lotte Coelmont, Francesco Dal Dosso, Kai Dallmeier, Jeroen Lammertyn
A novel platform, called SeParate, enabling accurate multiplex droplet sorting by integrating serial and parallel sorting principles for three model systems with increasing complexity and intra-subpopulation variation in fluorescence intensities.
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Lab Chip, 2024, 24,2039-2048
DOI: 10.1039/D4LC00073K, Paper

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Georgeta Vulpe, Guoyi Liu, Sam Oakley, Guanghao Yang, Arjun Ajith Mohan, Mark Waldron, Sanjiv Sharma
Development of wearable sensing devices for minimally invasive and real time monitoring of physiological information in ambulatory conditions.
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Lab Chip, 2024, 24,2080-2093
DOI: 10.1039/D3LC00838J, Paper

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Katharina Schlünder, Madalena Cipriano, Aline Zbinden, Stefanie Fuchs, Torsten Mayr, Katja Schenke-Layland, Peter Loskill
Pancreatic in vitro models are crucial for research on diseases such as diabetes mellitus. A novel thermoplastic organ-on-chip with integrated real-time read-outs models the complex microphysiological structure and function of the endocrine pancreas.
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Lab Chip, 2024, 24,2025-2038
DOI: 10.1039/D3LC01044A, Paper

Qiyue Luan, Ines Pulido, Angelique Isagirre, Julian Carretero, Jian Zhou, Takeshi Shimamura, Ian Papautsky
Agarose microwell platform for modeling lung carcinoma using patient-derived tissues to decipher cancer associated fibroblast-induced drug resistance.
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Lab Chip, 2024, 24,2069-2079
DOI: 10.1039/D3LC00830D, Paper

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Chaeeun Lee, Sumit Kumar, Juhee Park, Yongjun Choi, Elizabeth Maria Clarissa, Yoon-Kyoung Cho
Tonicity-induced cargo loading into extracellular vesicles (EVs) is achieved through controlled membrane permeability, ensuring cargo incorporation without causing damage to the EV membrane.
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Lab Chip, 2024, 24,1833-1866
DOI: 10.1039/D4LC00089G, Critical Review

Sasikala Apoorva, Nam-Trung Nguyen, Kamalalayam Rajan Sreejith
Wearable devices are increasingly popular in health monitoring, diagnosis, and drug delivery. Advances allow real-time analysis of biofluids like sweat, tears, saliva, wound fluid, and urine.
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Lab Chip, 2024, 24,1947-1956
DOI: 10.1039/D3LC00656E, Paper

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Klaudia Staskiewicz, Maria Dabrowska-Zawada, Lukasz Kozon, Zofia Olszewska, Lukasz Drewniak, Tomasz S. Kaminski
Novel microfluidic technique for functional selection of biosurfactant-producing microorganisms. Single bacterial cells are encapsulated into picoliter droplets for clonal cultivation and passively sorted at high throughput by interfacial tension.
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Lab Chip, 2024, 24,1867-1874
DOI: 10.1039/D3LC00796K, Perspective

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Umut A. Gurkan, David K. Wood, Dorn Carranza, Luke H. Herbertson, Scott L. Diamond, E. Du, Suvajyoti Guha, Jorge Di Paola, Patrick C. Hines, Ian Papautsky, Sergey S. Shevkoplyas, Nathan J. Sniadecki, Vamsee K. Pamula, Prithu Sundd, Asif Rizwan, Pankaj Qasba, Wilbur A. Lam
In this perspective article, we present the state of the microfluidic field regarding current limitations and propose future directions and new approaches for the field to advance lab-on-a-chip technologies closer to translation and clinical use.
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Lab Chip, 2024, 24,2094-2106
DOI: 10.1039/D3LC01082A, Paper

Open Access

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Claudia Olaizola-Rodrigo, Sujey Palma-Florez, Teodora Ranđelović, Clara Bayona, Mehran Ashrafi, Josep Samitier, Anna Lagunas, Mònica Mir, Manuel Doblaré, Ignacio Ochoa, Rosa Monge, Sara Oliván
A novel methodology utilizing plasma surface treatment enables the construction of cell culture chambers featuring abutment-free patterns, facilitating the precise distribution of shear stress.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00006D, Paper

Seyedaydin Jalali, Ponnambalam Ravi Selvaganapathy
A scalable method for creating perfusable 3D tissue constructs using self-assembly is introduced. Unlike conventional cell injection methods, here we leverage cell migration to form tissue barriers of low permeability, mimicking in vivo environments.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC00743J, Tutorial Review

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Olivia M. Young, Xin Xu, Sunandita Sarker, Ryan D. Sochol
This Tutorial Review highlights strategies for leveraging the micron-to-submicron-scale additive manufacturing technique, “direct laser writing”, to enable 3D microfluidic technologies.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC00891F, Paper

Yu-Hsiang Hsu, Wen-Chih Yang, Yi-Ting Chen, Che-Yu Lin, Chiou-Fong Yang, Wei-Wen Liu, Subhashree Shivani, Pai-Chi Li
The DC-MPS device uses a velocity V-clamp to create a partition with a steep concentration gradient for simultaneously developing vasculogenic vessels and a tumor. It enables vessel–tumor interaction to develop a tumor model with angiogenic vessels.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC00933E, Paper

Open Access

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Huynh Quoc Nguyen, Van Dan Nguyen, Vu Minh Phan, Tae Seok Seo
The COVID-19 pandemic greatly impacted the in vitro diagnostic market, leading to the development of new technologies such as point-of-care testing (POCT), multiplex testing, and digital health platforms.
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D4LC00118D, Paper

Open Access

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Jiaheng Li, Lok Ting CHU, Hogi Hartanto, Guihuan Guo, Lu LIU, Jianpeng WU, Minghui Wu, Chenyu Cui, Gaobo Wang, Wengang LIU, Hoi Kwan Kwong, Siying WU, Ting-Hsuan Chen
Mucosal antibodies at the upper respiratory tract are the earliest and most critical responders to prevent respiratory infections, providing an indication to rapidly evaluate immune protection. Here, we report a...
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00131A, Paper

Fahima Akther, Hedieh Fallahi, Jun Zhang, Nam-Trung Nguyen, Hang Thu Ta
An atherothrombosis-on-chip model to evaluate thrombosis risk and patient-specific treatment strategy.
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D3LC01062G, Paper

Open Access

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Steven A. Soper, Chathurika Rathnayaka, Indu Chandrasoma, Junseo Choi, Katie Childers, Maximillian Chibuike, Khurshed Akabirov, Farhad Shiri, Adam R Hall, Collin J McKinney, Matthew David Verber, Sunggook Park
We report the generation of ~8 nm dual in-plane pores fabricated in a thermoplastic via nanoimprint lithography (NIL). These pores were connected in series with nanochannels, one of which served...
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC01061A, Paper

Open Access

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Felix Reichel, Ruchi Goswami, Salvatore Girardo, Jochen Guck
We use hyperbolic microchannels to create a flow field with linearly increasing velocity, leading to a constant tensile stress to probe the viscoelastic properties of cells. We verify our approach on oil droplets and polyacrylamide beads.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC01101A, Paper

Wei Wang, Jaime de la Fuente Diez, Nicolas Delsuc, Juan Peng, Riccardo Spezia, Rodolphe Vuilleumier, Yong Chen
A new method uses piezoelectric and microfluidic tuning to study vibrational polaritons in liquids under flow. This enables modifying cavity length adjustments and flexibility in experimental setups.
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D3LC01066J, Paper

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Artur Davletshin, Wen Song
Fluid-solid interactions in nanoporous materials underlie processes fundamental to nature and the engineered environment, including the thermochemical transformation of argillaceous materials during high-level nuclear waste disposal. Operando fluid-solid resolution at...
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D3LC00854A, Paper

Lu Xin, Xi Xiao, Wen Xiao, Ran Peng, Hao Wang, Feng Pan
The incidence of urothelial carcinoma continue to rise annually, particularly among the elderly. Prompt diagnosis and treatment can significantly enhance patient survival and quality of life. Urine cytology remains a...
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00165F, Paper

Xiaoming Chen, Shun Liu, Mo Shen, Jishun Shi, Chungang Wu, Zhipeng Song, Yong Zhao
We develop a microfluidic device with closed right-angle bipolar electrodes to realize dielectrophoretic characterization and selection of non-spherical flagellate algae.
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Lab Chip, 2024, 24,2176-2192
DOI: 10.1039/D3LC00912B, Paper

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Priscilla Delgado, C. Alessandra Luna, Anjana Dissanayaka, Oluwamayokun Oshinowo, Jesse J. Waggoner, Sara Schley, Todd Fernandez, David R. Myers
This activity enables students to construct geometrically complex microfluidics at their desk with no specialized equipment. It can be easily transported, and helped students significantly improve their understanding of miniaturization science.
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Lab Chip, 2024, 24,2224-2236
DOI: 10.1039/D3LC00714F, Paper

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Simon Seidel, Katja F. Winkler, Anke Kurreck, Mariano Nicolas Cruz-Bournazou, Katharina Paulick, Sebastian Groß, Peter Neubauer
This publication introduces a thermal segment microwell plate control device, integrating smart sensor technology and standardized well-plate geometry for automated biolab setups. It is tested in a high-throughput enzymatic activity assay.
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Lab Chip, 2024, 24,2193-2201
DOI: 10.1039/D3LC00979C, Paper

Bingbing Zhang, Jinxin Fu, Maohua Du, Kai Jin, Qi Huang, Jiahao Li, Dongping Wang, Siyi Hu, Jinhua Li, Hanbin Ma
Advancing active-matrix digital microfluidics through polar coordinates for precise concentration gradients. Demonstrated with chip photography, concentration gradient solution demo, and efficiency comparison.
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Lab Chip, 2024, 24,2253-2261
DOI: 10.1039/D3LC01053H, Paper

Ziran Ye, Ping Lu, Yiben Chen, Zhixian Xu, Haixia Huang, Mingjia Zhi, Zi Ang Chen, Bo Yan
We present an efficient approach for the consecutive synthesis of Au–TiO₂ nanocomposites with controlled morphologies in a microfluidic chip.
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Lab Chip, 2024, 24,2237-2252
DOI: 10.1039/D3LC00694H, Paper

Open Access

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Nilay Vora, Prashant Shekar, Taras Hanulia, Michael Esmail, Abani Patra, Irene Georgakoudi
We present a deep-learning enabled, label-free flow cytometry platform for identifying circulating tumor cell clusters in whole blood based on the endogenous scattering detected at three wavelengths. The method has potential for in vivo translation.
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Lab Chip, 2024, 24,2280-2286
DOI: 10.1039/D3LC00822C, Paper

Mingwei Zhao, Jing Yang, Zhenqing Li, Yuan Zeng, Chunxian Tao, Bo Dai, Dawei Zhang, Yoshinori Yamaguchi
Concentration gradient generation and mixed combinations of multiple solutions are of great value in the field of biomedical research.
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Lab Chip, 2024, 24,2262-2271
DOI: 10.1039/D4LC00055B, Paper

Kawin Khachornsakkul, Ruben Del-Rio-Ruiz, Lita Chheang, Wenxin Zeng, Sameer Sonkusale
A carbon dots and dopamine-imprinted polymer was immobilized onto a distance-based paper channel for simultaneous detection of three cytokine biomarkers without requiring expensive reagents and instruments.
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Lab Chip, 2024, 24,2202-2207
DOI: 10.1039/D4LC00014E, Paper

Matthew S. Viglione, Aubrianna Saxton, Dawson Downs, Adam T. Woolley, Kenneth A. Christensen, Pam M. Van Ry, Gregory P. Nordin
A new 3D printing method allows for fabrication of porous membranes with 7 μm pores. A device mimicking an organ-on-a-chip design is fabricated and seeded with living cells, demonstrating potential for diverse biological and mechanical applications.
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Lab Chip, 2024, 24,2146-2175
DOI: 10.1039/D3LC01086D, Critical Review

Uditha Roshan, Amith Mudugamuwa, Haotian Cha, Samith Hettiarachchi, Jun Zhang, Nam-Trung Nguyen
This review paper provides a comprehensive overview of the state-of-the-art actuation mechanisms for flexible and stretchable microdevices.
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Lab Chip, 2024, 24,2317-2326
DOI: 10.1039/D4LC00151F, Paper

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Amirreza Gholivand, Olivera Korculanin, Knut Dahlhoff, Mehrnaz Babaki, Timo Dickscheid, Minne Paul Lettinga
Taking microfluidics to the third dimension causes scrambling of memory effects in blood flow due to out-of-plane vessel structures.
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Lab Chip, 2024, 24,2358-2359
DOI: 10.1039/D4LC90026J, Correction

Open Access

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Lei Mou, Kalpana Mandal, Marvin Magan Mecwan, Ana Lopez Hernandez, Surjendu Maity, Saurabh Sharma, Rondinelli Donizetti Herculano, Satoru Kawakita, Vadim Jucaud, Mehmet Remzi Dokmeci, Ali Khademhosseini
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Lab Chip, 2024, 24,2272-2279
DOI: 10.1039/D3LC01079A, Paper

Huilan Hu, Jiaqi Tian, Rui Shu, Huihui Liu, Shaochi Wang, Xuechi Yin, Jianlong Wang, Daohong Zhang
A lateral flow immunoassay (LFIA) is developed for enzyme-catalyzed double-reading determination of clenbuterol (CLE) through the direct electrostatic adsorption of ultra-small copper–gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies.
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Lab Chip, 2024, 24,2287-2297
DOI: 10.1039/D3LC00866E, Paper

Arata Tsuchida, Taikopaul Kaneko, Kaori Nishikawa, Mayu Kawasaki, Ryuji Yokokawa, Hirofumi Shintaku
We introduce a simple integrated analysis method that links cellular phenotypic behaviour with single-cell RNA sequencing by utilizing a combination of optical indices from cells and hydrogel beads.
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Lab Chip, 2024, 24,2347-2357
DOI: 10.1039/D3LC00834G, Paper

Austin Lai, Adam Hawke, Mokhaled Mohammed, Peter Thurgood, Gianmarco Concilia, Karlheinz Peter, Khashayar Khoshmanesh, Sara Baratchi
We created a versatile microfluidic system for studying the effect of arrhythmic flow patterns on endothelial cells in vitro. This system provides unique opportunities to identify the molecular machineries that control endothelial biology in response to arrhythmia.
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Lab Chip, 2024, 24,2327-2334
DOI: 10.1039/D4LC00176A, Paper

Open Access

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Aravind M, Sajan D. George
Adhesive contrast contact lens fabricated by selective lubricant grafting splits tear film on specified zone by secretion and blinking. Integrating the contact lens with the spectroscopic technique offers an efficient platform for biomolecule sensing.
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Lab Chip, 2024, 24,2298-2305
DOI: 10.1039/D3LC00730H, Paper

Mahdi Samadi Khezri, Mohammad Reza Housaindokht, Mojtaba Firouzi
A volumetric bar-chart chip (V-chip) is a microfluidic device based on distance-based quantitative measurement that visualizes analyte concentration without the need for apparatus or data processing.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC00931A, Perspective

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Fu Der Chen, Ankita Sharma, David A. Roszko, Tianyuan Xue, Xin Mu, Xianshu Luo, Hongyao Chua, Patrick Guo-Qiang Lo, Wesley D. Sacher, Joyce K. S. Poon
We explore the development of multifunctional nanophotonic neural probes, integrating microelectrodes, optical emitters, and microfluidic channels for multimodal brain mapping.
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Lab Chip, 2024, 24,2306-2316
DOI: 10.1039/D3LC01045G, Paper

Sabah H. Al-Jaf, Sameera Sh. Mohammed Ameen, Khalid M. Omer
On-site monitoring of ions in drinking water is essential for safeguarding public health, ensuring high water quality, and preserving the ecological balance of aquatic ecosystems.
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Lab Chip, 2024, 24,2208-2223
DOI: 10.1039/D3LC01055D, Paper

Open Access

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Jungseub Lee, Sangmin Jung, Hye Kyoung Hong, Hyeonsu Jo, Stephen Rhee, Ye-Lin Jeong, Jihoon Ko, Yong Beom Cho, Noo Li Jeon
VT-MAP, a novel in vitro platform, offers better physiological reflection through the vascularized organoid. This advancement supports drug assessment with deeper insights into cancer biology through correlation between morphology and drug effects.
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Lab Chip, 2024, 24,2335-2346
DOI: 10.1039/D4LC00032C, Paper

Rodin Chermat, Elena Refet-Mollof, Yuji Kamio, Jean-François Carrier, Philip Wong, Thomas Gervais
We introduce the first brachytherapy-on-chip device for in vitro testing of brachytherapy on naturally hypoxic tumor spheroids, for translational research and drug development.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00087K, Paper

Mohammad Mahdi Mardanpour, Ayyappasamy Sudalaiyadum Perumal, Zahra Mahmoodi, Karine Baassiri, Gala Montiel-Rubies, Kenneth M. LeDez, Dan V. Nicolau
This study conducted in vitro experimentation using microvascular tissue-like structures to examine the dynamics of interfacial forces leading to gas bubble cessation, with the objective of providing a medical guideline for preventing gas embolism.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00012A, Paper

Anna L. Butterworth, Matin Golozar, Zachary Estlack, Jeremy McCauley, Richard A. Mathies, Jungkyu Kim
This work presents the design, fabrication, and automation of our space flight-format microfabricated sample processing and capillary electrophoresis analysis instrument.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC01052J, Paper

Wenxin Wang, Yiyuan Liu, Zhikai Yao, Dengbo Chen, Yue Tang, Jingwei Cui, Jiangjiang Zhang, Hong Liu, Zikai Hao
A depression-on-gut-chip (DoGC) is prepared and enables extended co-culture of aerobic human intestinal epithelial cells and anaerobic gut microbiota, and allows direct study of interactions between human gut microbiota and depression.
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D4LC00159A, Paper

Lixue Liang, Xueying Wang, Dong Chen, Palaniappan Sethu, Guruprasad A. Giridharan, Yanxia Wang, Yu Wang, Kai-Rong Qin
The best pulsation frequency mode of the RBP would be one that changes every 2–3 times of the cardiac cycle. The proposed in vitro microfluidic model could provide an effective platform to select the best working mode of the RBP for heart failure.
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D4LC00291A, Paper

Nana Jin, Fan Jiang, Fengzhen Yang, Ying Ding, Ming Liao, Yanbin Li, Jianhan Lin
A colorimetric biosensor was elaboratively designed for fast, sensitive and multiplex bacterial detection on a single microfluidic chip using immune magnetic nanobeads for specific bacterial separation, immune gold@platinum palladium nanoparticles...
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC01116J, Paper

Duomei Tian, Zheng Mao, Li Wang, Xiaochen Huang, Wei Wang, Haoyue Luo, Juan Peng, Yong Chen
Tumor spheroids are cultured in hydrogel microwells with a gravity driven medium flow underneath the membrane on-a-chip. The rocking system is pumpless, powered with a rechargeable battery, and programmable with a built-in microprocess.
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D3LC00941F, Paper

Shizheng Zhou, Tianhui Chen, Edgar S. Fu, Liuyong Shi, Teng Zhou, Hong YAN
The composition of species and the physiological status of microalgal cells serve as significant indicators for monitoring marine environments. Symbiotic with corals, Symbiodiniacea are more sensitive to the environmental response....
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Lab Chip, 2024, Accepted Manuscript
DOI: 10.1039/D4LC00105B, Critical Review

Shanqing Huang, Weixiong Shi, Shiyu Li, Qian Fan, Chaoyong Yang, Jiao Cao, Lingling Wu
Cells are the fundamental building blocks of living systems, exhibiting significant heterogeneity. The transcriptome connects the cellular genotype and phenotype, and profiling single-cell transcriptome is critical for uncovering distinct cell...
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Lab Chip, 2024, Advance Article
DOI: 10.1039/D3LC00985H, Paper

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Nan Jia, Tianyang Deng, Charles Larouche, Tigran Galstian, André Bégin-Drolet, Jesse Greener
We report a flow sensitive birefringent in-channel chitosan micromembrane. Using a simple cross-polarizer and a calibration curve, the membrane functions as a flow rate meter, which also works as a feedback element for a flow control system.
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