Actor and "The Passion of the Christ" director Mel Gibson recently revealed his support for former President Donald Trump and his belief that Vice President Kamala Harris sports both an "appalling track record" and a low IQ.

Known to millions as the drummer of the Grammy Award-winning band Hootie & the Blowfish, Jim “Soni” Sonefeld once appeared to have it all — a successful career, a loving family and a public image that masked his private struggles. Beneath the surface, however, he grappled with inner demons.

While there’s been no shortage of reactions to the recent Joe Rogan interview with former President Donald Trump, filmmaker and Blaze TV host Steve Deace says he believes the podcast will prove to be nothing short of historic. 

Academy Award-winning filmmaker Martin Scorsese is embarking on a spiritual journey through the lives of revered saints, from Joan of Arc to John the Baptist, with his latest docudrama series, “The Saints,” premiering next month.

Grammy Award-winning artist Lauren Daigle has written and recorded a powerful end-title track for the new film “Bonhoeffer: Pastor. Spy. Assassin” declaring obedience to God’s calling, even in the face of death. 

Dallas Jenkins, creator of "The Chosen," shares how his new film, "The Best Christmas Pageant Ever," brings the Gospel story to a mainstream audience.

With the release of Skillet's latest album, "Revolution," lead singer John Cooper is more impassioned than ever, sounding an alarm over what he sees as a pivotal moment in America’s history. 

Hollywood actor Chris Pratt has signed on to “Fighting Spirit: A Combat Chaplain’s Journey” as an executive producer, joining forces with director Rich Hull and former combat chaplain Justin D. Roberts. 

Two Christian activists urged fellow believers to remain active in politics, contending that they have an obligation to become “major influencers” in American society by restoring civility to political discourse. 

The latest entry in the fantasy role-playing franchise 'Dragon Age' allows players to make their character trans-identifying and features a scene where one character is forced to atone for “misgendering” someone by performing a series of push-ups. 

Christmas came early as stars, filmmakers and fans gathered for the red carpet premiere of “The Best Christmas Pageant Ever” — a family-friendly film creators hope will earn a spot among the holiday classics. 

“Full House” alum Candace Cameron Bure has encouraged her followers to “vote like Jesus” on Election Day as the race between former President Donald Trump and Vice President Kamala Harris nears the finish line.

In a world fractured by deep ideological divisions, Nicholas Ma’s new documentary, “Leap of Faith,” seeks to answer the question of whether love can bridge seemingly unbridgeable divides. 

Late night comedy talk show host Jimmy Kimmel was reduced to tears Wednesday as he scolded the voters who elected President-elect Donald Trump to a second term.

The documentarian who recently went viral when Tucker Carlson told him he was "physically mauled" by a demon last year pushed back against those who would dismiss the former Fox News host's claims about spiritual warfare.

The Recording Academy announced nominations for the 67th annual Grammy Awards on Thursday, with Christian and gospel artists such as Bethel Music, Brandon Lake, CeCe Winans and newcomer Forest Frank receiving nods.

Actor John Rhys-Davies narrates a reimagined audio drama of "A Christmas Carol," drawn by the story’s themes of transformation, compassion and redemption, which he likens to the journeys of both Scrooge and his Rings character, Gimli, as they overcome prejudice and open their hearts to others.

As the Herdman kids learn, Christmas celebrates God’s arrival not in a palace but in a humble manger, for the sake of all people, including those we may least expect.

On Dec. 6th, Netflix invites audiences on a compelling journey back to ancient Judea to witness the story of the Nativity through the eyes of Mary of Nazareth, mother of Jesus Christ, in the film “Mary.” 

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 That's how it started: www.facebook.com/photo.php?v=273122249390826&l=3365249543954861233

Have the opportunity to download free sound files in "Products" , but to get the musical material they require registration fen.She required to have at Vip Account whose year value is 50 euros.

Hello Everyone! Today, August 16, 2013 is the day that this site was created and has been edited for the first time ! Here you may find my own compositions and arrangements, many will also have have the chance to hear my performances at the piano. All of this can be found on page "Products" section of this site.I hope is to have fun!

On December 20 in Sofia, Bulgaria held a charity concert for financial assistance of Eleonora-piano teacher suffered from severe physical abuse with many operational quantity treatments for which she did not have enough money.  The concert was held at the Russian Cultural and Information Centre in Sofia and the main organizer and performer of the works of the piano was Pavlin Stanchev, the author of this site and all of it displayed in musical products.

Discover if Cancer and Pisces compatibility creates a strong, harmonious bond. Dive into emotional insights and learn what makes these water signs connect so deeply.

Soccer, football — no matter what you call it, the world's most popular sport has a long and colorful history, from its ancient origins to its modern-day status as a global phenomenon. But when was soccer invented? How did it become the "beautiful game" that captivates millions today?

Thrilling gameplay, dramatic storylines and top-notch talent are all important factors in ensuring a sport's popularity grows and fanbases thrive. The most popular sports in the world are all spectacles of athletic competition that attract large crowds in the stands and around the television.

Discover how the 7th House in astrology shapes love, partnerships, and marriage dynamics, revealing deep insights into relationship compatibility and commitment.

Explore Virgo men's traits, compatibility, and relationship dynamics. Learn what makes a Virgo man unique in love and if he's the right zodiac match for you.

Discover the compatibility between Cancer and Aquarius in love, friendship, and relationships. Learn how their emotional and intellectual traits align or clash.

Explore Leo and Pisces compatibility, uncovering their strengths, weaknesses, and whether these zodiac signs can make their relationship work.

Explore the Aquarius Moon's traits, emotional depth, and how it influences relationships, creativity, and personal growth in astrology. Unlock its mysterious power.

Discover Taurus and Virgo compatibility in love, relationships, and friendship. Explore how these earth signs align in values, trust, and practical connections.

s Virgo and Capricorn compatibility strong? Discover how these earth signs connect in love, friendship, and marriage with shared values, loyalty, and mutual support.

Is Aquarius woman unique? Discover her free-spirited personality, loyalty in friendship, innovative mindset, and how she navigates love and relationships.

Explore Aquarius Season's unique energy, from innovation to social connection. Learn how this zodiac period influences creativity, relationships, and self-expression.

Discover the spiritual meaning of angel number 911. Learn how 911 symbolizes growth, change, and guidance from the universe in life, love, and personal journeys.

Discover Aries and Sagittarius compatibility in love, friendship, and beyond. Learn how these two fire signs create a dynamic, passionate bond full of excitement and adventure.

Discover the meaning of the Ace of Swords reversed in tarot. Explore its insights on clarity, communication, and inner conflicts in love, career, and personal growth.

Is Gemini and Capricorn compatibility strong? Discover the unique dynamics, strengths, and challenges that Gemini and Capricorn face in love, friendship, and beyond.

Explore reversed Pisces and Cancer compatibility! Learn how these water signs bond emotionally, support each other deeply, and thrive together in relationships.

Is Ace of Cups Reversed" reveals insights into blocked emotions and self-reflection in tarot readings, offering guidance on relationships and personal growth.

Explore Cancer and Taurus compatibility, where stability meets sensitivity. Learn how these signs create lasting love through trust, loyalty, and mutual understanding.

Discover the unique traits of each zodiac element—fire, earth, air, and water. Uncover how these elements shape personalities and compatibility in astrology.

Conjugation is the process by which plasmids, including those that carry antibiotic-resistance genes, are mobilized from one bacterium (the donor) to another (the recipient). The conjugation efficiency of IncF-like plasmids relies on the formation of mating-pair stabilization via intimate interactions between outer membrane proteins on the donor (a plasmid-encoded TraN isoform) and recipient bacteria. Conjugation of the R100-1 plasmid into Escherichia coli and Klebsiella pneumoniae (KP) recipients relies on pairing between the plasmid-encoded TraNα in the donor and OmpW in the recipient. Here, the crystal structure of K. pneumoniae OmpW (OmpWKP) is reported at 3.2 Å resolution. OmpWKP forms an eight-stranded β-barrel flanked by extracellular loops. The structures of E. coli OmpW (OmpWEC) and OmpWKP show high conservation despite sequence variability in the extracellular loops.

Protein tyrosine phosphatase 1B (PTP1B) plays important roles in cellular homeostasis and is a highly validated therapeutic target for multiple human ailments, including diabetes, obesity and breast cancer. However, much remains to be learned about how conformational changes may convey information through the structure of PTP1B to enable allosteric regulation by ligands or functional responses to mutations. High-resolution X-ray crystallography can offer unique windows into protein conformational ensembles, but comparison of even high-resolution structures is often complicated by differences between data sets, including non-isomorphism. Here, the highest resolution crystal structure of apo wild-type (WT) PTP1B to date is presented out of a total of ∼350 PTP1B structures in the PDB. This structure is in a crystal form that is rare for PTP1B, with two unique copies of the protein that exhibit distinct patterns of conformational heterogeneity, allowing a controlled comparison of local disorder across the two chains within the same asymmetric unit. The conformational differences between these chains are interrogated in the apo structure and between several recently reported high-resolution ligand-bound structures. Electron-density maps in a high-resolution structure of a recently reported activating double mutant are also examined, and unmodeled alternate conformations in the mutant structure are discovered that coincide with regions of enhanced conformational heterogeneity in the new WT structure. These results validate the notion that these mutations operate by enhancing local dynamics, and suggest a latent susceptibility to such changes in the WT enzyme. Together, these new data and analysis provide a detailed view of the conformational ensemble of PTP1B and highlight the utility of high-resolution crystallography for elucidating conformational heterogeneity with potential relevance for function.

Owing to the difficulties associated with working with carbohydrates, validating glycan 3D structures prior to deposition into the Protein Data Bank has become a staple of the structure-solution pipeline. The Privateer software provides integrative methods for the validation, analysis, refinement and graphical representation of 3D atomic structures of glycans, both as ligands and as protein modifiers. While Privateer is free software, it requires users to install any of the structural biology software suites that support it or to build it from source code. Here, the Privateer web app is presented, which is always up to date and available to be used online (https://privateer.york.ac.uk) without installation. This self-updating tool, which runs locally on the user's machine, will allow structural biologists to simply and quickly analyse carbohydrate ligands and protein glycosylation from a web browser whilst retaining all confidential information on their devices.

Human tRNA (uracil-5-)-methyltransferase 2 homolog A (TRMT2A) is the dedicated enzyme for the methylation of uridine 54 in transfer RNA (tRNA). Human TRMT2A has also been described as a modifier of polyglutamine (polyQ)-derived neuronal toxicity. The corresponding human polyQ pathologies include Huntington's disease and constitute a family of devastating neuro­degenerative diseases. A polyQ tract in the corresponding disease-linked protein causes neuronal death and symptoms such as impaired motor function, as well as cognitive impairment. In polyQ disease models, silencing of TRMT2A reduced polyQ-associated cell death and polyQ protein aggregation, suggesting this protein as a valid drug target against this class of disorders. In this paper, the 1.6 Å resolution crystal structure of the RNA-recognition motif (RRM) from Drosophila melanogaster, which is a homolog of human TRMT2A, is described and analysed.

Sorghum, a short-day tropical plant, has been adapted for temperate grain production, in particular through the selection of variants at the MATURITY loci (Ma1–Ma6) that reduce photoperiod sensitivity. Ma3 encodes phytochrome B (phyB), a red/far-red photochromic biliprotein photoreceptor. The multi-domain gene product, comprising 1178 amino acids, autocatalytically binds the phytochromobilin chromophore to form the photoactive holophytochrome (Sb.phyB). This study describes the development of an efficient heterologous overproduction system which allows the production of large quantities of various holoprotein constructs, along with purification and crystallization procedures. Crystals of the Pr (red-light-absorbing) forms of NPGP, PGP and PG (residues 1–655, 114–655 and 114–458, respectively), each C-terminally tagged with His6, were successfully produced. While NPGP crystals did not diffract, those of PGP and PG diffracted to 6 and 2.1 Å resolution, respectively. Moving the tag to the N-terminus and replacing phytochromobilin with phycocyanobilin as the ligand produced PG crystals that diffracted to 1.8 Å resolution. These results demonstrate that the diffraction quality of challenging protein crystals can be improved by removing flexible regions, shifting fusion tags and altering small-molecule ligands.

The GTPase FlhF, a signal recognition particle (SRP)-type enzyme, is pivotal for spatial–numerical control and bacterial flagella assembly across diverse species, including pathogens. This study presents the X-ray structure of FlhF in its GDP-bound state at a resolution of 2.28 Å. The structure exhibits the classical N- and G-domain fold, consistent with related SRP GTPases such as Ffh and FtsY. Comparative analysis with GTP-loaded FlhF elucidates the conformational changes associated with GTP hydrolysis. These topological reconfigurations are similarly evident in Ffh and FtsY, and play a pivotal role in regulating the functions of these hydrolases.