Introduced in 1934, the federal duck stamp contest has raised more than $1.2 billion and protected at least 6.5 million acres across the nation. Now, an art exhibition at Connecticut’s Bruce Museum honors the competition’s history

The species may have been carried to the Sacramento–San Joaquin Delta in ballast water on ships

The Manhattan district attorney's office has obtained an arrest warrant for Edoardo Almagià, who has been accused of working with looters and dealing stolen artifacts for years

When the war ended, Allied soldiers tracked down Nicolas de Largillierre's "Portrait de femme à mi-corps" with the help of a savvy French curator who had been working for the resistance

Steve Lazarides' personal collection of prints, original works, handwritten press releases and burner phones sold at auction for around $1.4 million

Lula the bear and Simba the lion have been evacuated to a wildlife shelter in Jordan

An increasing number of people in France espouse a clothing-free lifestyle

This story and others have come to light during a project to translate and digitize a series of texts about archbishops in York, England

Sharks have been losing teeth for 400 million years. Here’s a guide to uncovering some of these plentiful fossils across the country

Michelle Frankfurter tells the stories of these young migrants and also those of the thousands who jump aboard “the death train”

Police have reported that a man was fatally electrocuted in Torbay on Saturday evening. Two others are being treated for injuries.

Bombardier control box recovered during second dive to a World War 2 bomber at the bottom of Gander Lake.

Kate MacEachern is a Canadian veteran from Ballantynes Cove, N.S., but her service years are far from over. She has spent about two years living in Ukraine, delivering humanitarian aid to communities across the country.

Dominic April fell in love with forging after his career in the military came to an end in 2015. Now operating a blacksmith studio near Quebec City, he hopes to help other veterans dealing with PTSD find creativity and purpose.

To understand the circuitry of the brain, it is often advantageous to visualize the processes of a single neuron or population of neurons. Identifying sites where a neuron, or neurons, originates and where it projects can allow a researcher to begin to map the circuitry underlying various processes, including sensory-guided behaviors. Furthermore, neural tracing allows one to map locations where processes terminate onto regions of the brain that may have known functions and sometimes to identify candidate upstream or downstream connections, based on proximity. Many methods of neural tracing are available; here, we focus on loading fluorescent dyes into a neuron (fluorescent dye filling). Different options for dyes exist to label neurites. Among the most versatile and easy to use are dextran amine–conjugated dyes. They fill neurons bidirectionally, not discriminating between anterograde or retrograde loading direction. Dye filling must be done in unfixed tissue, as the dye needs to move through the neurons; however, dextran amine conjugates are aldehyde-fixable and once cells have been fully loaded with dye the tissue can be fixed and subjected to immunostaining. Coupling neural tracing with immunofluorescence is a useful way to determine specific brain or ventral nerve cord (VNC) regions where a neuron projects. This protocol describes methods for loading dextran amine conjugated dyes into a sensory tissue in the mosquito to visualize sites of sensory neuron innervation in the central nervous system, as well as efferent projections to these structures. This protocol is described for Aedes aegypti, for which it was optimized, but it also works across a variety of insects.

All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is collected and the extraction of DNA. As soon as a mosquito ingests blood from a host animal, digestion of host cells and cellular components within the blood meal by enzymes in the mosquito midgut begins to degrade the host DNA templates that are the targets of polymerase chain reaction (PCR) amplification. Without effective preservation, host DNA is typically undetectable by PCR 48 h after feeding, because of digestion. Preservation methods for mosquito blood meals vary in their efficacy, and the logistics of fieldwork can limit the options for preservation of blood meals and maintenance of the integrity of host DNA. This protocol describes a method of blood meal preservation that is effective, convenient, and amenable to fieldwork in remote locations where cryopreservation at –20°C or –80°C may not be feasible. It uses a Flinders Technology Associates (FTA) card, which is a chemically treated card that lyses cells and allows nucleic acids to be preserved. This method is also expected to preserve the DNA or RNA of pathogens present within the engorged mosquito abdomen, including RNA viruses.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

Hay River RCMP say they believe two weekend incidents, including one in which a person was shot, are linked to drugs and organized crime.

A group of volunteers have been providing emergency services to West Dawson residents during the periods of freeze up and break up, when there is no reliable access across the Yukon River to town.

Historians say that Sarah Emma Edmonds exaggerated many aspects of her wartime experiences. Still, she bravely served in the Union Army, becoming one of hundreds of women who fought in the conflict in secret

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

An exhibition at the Huntington Library, Art Museum and Botanical Gardens explores how Western intellectuals viewed the climate crisis between 1780 and 1930

Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

Trevor Lewis was a corporal in the Canadian Armed Forces who tried to bury the memories from his deployment in Afghanistan. They all came back in 2021 after the fall of Kabul. This story is how he made peace with his time in that country.

A retired lawyer from Sault Ste. Marie has written a book that celebrates the working-class neighbourhood in which he grew up. The West End: A Magical Place Created by Giants is by Frank Sarlo, whose grandfather emigrated to Chicago from Italy, but ended up in Sault Ste. Marie with the promise of work on the railroad.

An 18-year-old man charged with attempted murder in a small northern Ontario community made a brief court appearance today by video, and remains in custody. Meanwhile, community members gathered outside the courthouse for a rally.

Benjamin Mora, a finalist for Canadian Premier League coach of the year, has left York United FC after five months and 22 matches at the helm.

In Foxboro Green, a small retirement community in Baden, Ont., 30 women came together to create a 15-metre-long banner covered in small handmade poppies. CBC Kitchener-Waterloo's Cameron Mahler went to where they've displayed the banner ahead of Remembrance Day to find out more.

A seed company based in Regina names all new plant varieties after veterans —something a 102-year-old Second World War veteran says is "a great honour."

The final election results are in for the 2024 Saskatchewan election, which received a higher vote turnout than 2020. The NDP's April ChiefCalf won Saskatoon-Westview. And Saskatchewan Party’s Ken Cheveldayoff was re-elected in Saskatoon-Willowgrove.

A Remembrance Day ceremony in Sarnia, Ont., was disrupted Monday, police say, by the presence and arrest of multiple alleged members of an outlaw motorcycle gang.  

According to investigators, a suspect went into a convenience store in the 300 block of Shepherd Street West just before 8 p.m. and showed a handgun, demanded money from the cashier and left with both cash and items. 

Kamloops RCMP say they are investigating the fire that destroyed the Red Bridge Thursday as an arson.

A handful of voting stations were temporarily closed due to power outages on Saturday morning during the final day of B.C.’s provincial election, while the only station in Dease Lake was fully closed because an election official had travel difficulties.

The re-elected chief of the Tk'emlúps te Secwépemc First Nation says she's hoping to forge connections within her community as she looks ahead to her next term.

Nikolaj Ehlers scored the winning goal 1:26 into overtime, and the Winnipeg Jets remained unbeaten this season with a 4-3 win over the Seattle Kraken on Thursday night.

What would you do if you'd just been dealt a devastating, extremely public defeat? If your answer is "drink wine in leggings," then Vice-President and former Democratic U.S. presidential candidate Kamala Harris can do you one better: Drink wine in leggings, sporting a messy bun, while playing Connect 4.

Statistics Canada data reviewed by CBC Toronto shows a dramatic shift in criminal outcomes in Ontario over the last decade. The majority of criminal cases in the province have ended with charges being withdrawn, stayed, dismissed or discharged before a decision at trial since 2020.

The Toronto Police Services Board unanimously approved a new five-year hiring plan for the force on Tuesday.

The provincial government is seeking contractors to restore hundreds of kilometres of land where fireguards were created during the 2023 wildfire season.

Charlottetown-born curler Brett Gallant says competing in the Olympics will be a dream come true for him and his fiancée, Jocelyn Peterman, even if COVID-19 protocols will make it difficult for them to see each other.

It's not a complete surprise that curling and other sports have been cancelled because of the COVID-19 pandemic, but it doesn't make it any easier on teams that had their hopes of representing Northern Ontario at a national curling level dashed.

Curling Canada, with the assistance of Own the Podium and the Canadian Olympic Committee, announced Rachel Homan and John Morris as the mixed doubles team for Canada competing at the Beijing Olympics.

Record revenue of $1.6 billion Record quarterly ADV of 28.3 million contracts - the second consecutive quarter of volume, open interest growth across every asset class CHICAGO, Oct. 23, 2024...

A researcher may have many reasons for wanting to establish new laboratory colonies from field-collected mosquitoes. In particular, the ability to study the diversity found within and among natural populations in a controlled laboratory environment opens up a wide range of possibilities for understanding how and why burdens of vector-borne disease vary over space and time. However, field-collected mosquitoes are often more difficult to work with than established laboratory strains, and considerable logistical challenges are involved in safely transporting field-collected mosquitoes into the laboratory. Here, we provide advice for researchers working with Aedes aegypti, Anopheles gambiae, and Culex pipiens, as well as notes on other closely related species. We provide guidance on each stage of the life cycle and highlight the life stages for which it is easiest to initiate new laboratory colonies for each species. In accompanying protocols, we provide methods detailing Ae. aegypti egg collection and hatching as well as how to transport larvae and pupae from the field.

Transposon-mediated transgenesis of mosquito vectors of disease pathogens followed the early success of transgenesis in the vinegar fly, Drosophila melanogaster. The P transposable element used in Drosophila does not function canonically in mosquitoes, and repeatable, routine transgenesis in mosquitoes was not accomplished until new transposable elements were discovered and validated. A number of distinct transposons were subsequently identified that mediate the introduction of exogenous DNA in a stable and heritable manner in mosquito species, including members of the genera Aedes, Anopheles, and Culex. The most versatile element, piggyBac, is functional in all of these mosquito genera, as well as in many other insects in diverse orders, and has been used extensively outside the class. Transposon-mediated transgenesis of recessive and dominant marker genes and reporter systems has been used to define functional fragments of gene control sequences, introduce exogenous DNA encoding products beneficial to medical interests, and act as "enhancer traps" to identify endogenous genes with specific expression characteristics.

Quick design turnaround and easy documentation process help UK-based company move products to market faster