In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

FSI Briefs No 3, April 2020. In response to the 2007-09 Great Financial Crisis (GFC), accounting standard setters introduced a new methodology to value loans based on expected credit losses (ECL). The previous approach, based on incurred losses, was viewed as procyclical and inconsistent with prudential objectives. In the wake of the Covid-19 pandemic, several prudential authorities and the Basel Committee on Banking Supervision (BCBS), introduced a series of measures to clarify how banks should consider various public and private debt relief programmes in their ECL estimates and in their calculation of regulatory capital. These measures are intended to incentivise banks to continue supporting the real economy, while reducing pressure on banks' ECL provisions, earnings and regulatory capital. Supervisory initiatives that provide capital relief should be augmented by severe constraints on the payment of dividends, bonuses and share buybacks. These joint actions will simultaneously expand banks' lending capacity and enhance their ability to absorb losses. Prudential authorities face difficult trade-offs as they confront the most severe economic crisis in modern times. Encouraging the use of flexibility in applicable accounting standards, while preserving market trust and transparency in the reported financial statements of banks, will be key in fostering both economic and financial stability.

This paper reviews post-crisis financial regulatory reforms, examines how they fit together and identifies open issues. Specifically, it takes stock of the salient new features of bank and CCP international standards within a unified analytical framework.

BIS Bulletin No 9, April 2020. By allowing banks to run down some of their buffers, policymakers are sending a strong signal about their resolve to lessen the economic fallout from the pandemic. Such prudential measures complement the main policy levers: monetary and fiscal instruments. To avoid a reduction in credit to the real economy, authorities need to ensure that banks have the capacity and willingness to make use of the flexibility afforded by the buffer release. Payout restrictions on banks and risk-sharing between banks and the public sector will be key. For banks to continue playing a positive role in the supply of funding during the recovery, they should maintain usable buffers for a long period, as losses from a severe recession will take time to materialise.

The National Book Festival Presents series was created to provide a book festival experience to lovers of the event on a year-round basis. Because these programs can no longer be held at the Library, we are offering a virtual multipart series, with authors who have written books about widespread diseases and the worldwide response to them.

Architects and planners from the Netherlands are advising coastal cities worldwide on how to live with water

Press release: Basel Committee invites comments on the design of a prudential treatment for crypto-assets, 12 December 2019.

No global real estate market despite higher price synchronisation and growing role of international investors, central banks find (Press release, 18 February 2020)

Basel Committee Press release "Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementationl", 27 February 2020.

CPMI Press release "How fintech can promote financial inclusion - a new report on the opportunities and challenges", 14 April 2020

Press release "Saudi G20 Presidency and the Bank for International Settlements (BIS) Innovation Hub invite global innovators to solve RegTech and SupTech challenges", 27 April 2020

BCBS Press release "Basel Committee issues progress report on banks' implementation of the 'Principles for effective risk data aggregation and reporting'", 29 April 2020

Rutgers' RUCDR Infinite Biologics received an amended emergency use authorization from the FDA late Thursday for the first SARS-CoV-2 coronavirus test that will allow people to collect their own saliva at home and send to a lab for results.

The American Geriatrics Society annually honors researchers, clinicians, educators, and emerging health professionals who have made outstanding contributions to high-quality, person-centered care for older people.

A protein shredder that occurs in cell membranes of brain cells apparently also indirectly regulates the fat metabolism.

With a discovery that could rewrite the immunology textbooks, an international group of scientists, including the teams of Bart Lambrecht, Martin Guilliams, Hamida Hammad, and Charlotte Scott (all from the VIB-UGent Center for Inflammation Research) identified a new type of antigen-presenting immune cell.

Will a nationalist president be undone by his underreaction to a foreign threat?

Fifty thousand jobs were lost in Nova Scotia in April, reflecting the devastating economic impact of the first full month of public health orders to prevent the spread of COVID-19.

Carmen Figueroa Sotelo has been interpreting all the news conferences for English audiences since the beginning of the pandemic in Quebec

The current deconfinement plan in the Montreal area could lead to a rapid increase in deaths in the city, according to findings by Quebec's public health institute. 

Overcoming scorching heat and little rain, experimental vineyards teach winemakers to cope with climate change

Earlier this week, Manitoba's premier announced $200 cheques for seniors to help pay for increased costs during the COVID-19 pandemic. But some wonder if there is a better way to help those in need.

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 

With something like 380 websites being created every minute, building an e-commerce shop can be a daunting task. It's easy to look at all of the success stories out there and think, "How could I possibly be that lucky?" However, the opportunity for growth in e-commerce is not slowing down. E-commerce sales are projected to grow 85 percent from their 2019 totals by 2022.

Merchants soon will be able to sell products on Google Shopping at no charge. Previously, they had to pay per click, but the cost was not fixed. There was no minimum, but they had to set a maximum for ad spend and Google would stop displaying their ads once the maximum was reached. Starting next week, search results on the Google Shopping tab will consist primarily of free product listings.

Sign makers in P.E.I. have been busy since the province announced its plans to ease back COVID-19 restrictions, as businesses are ordering signs and decals ahead of reopening.

Prices on P.E.I. for gas, heating oil and diesel all took a big jump upwards Friday.

With opposition parties continuing to call for the legislature to be convened, P.E.I. Premier Dennis King says that opportunity for them to examine the government’s spending is coming.

P.E.I. had the lowest unemployment rate in the country in April, but behind that seemingly strong showing are hiding problems in the labour market.

Officials with Maritime Pride Eggs say their Summerside, P.E.I., egg facility will cease processing operations on June 5.

All of P.E.I.'s 27 confirmed cases of COVID-19 are now considered recovered, said Chief Public Health Officer Dr. Heather Morrison.

Artists from Michigan and around the world are painting 50 murals in Flint to refocus the city's image on art rather than the lead-tainted water crisis.

Authorities investigating Flint's water crisis have used search warrants to seize from storage the state-owned mobile devices of former Gov. Rick Snyder and 65 other current or former officials, The Associated Press has learned.

Former Gov. Rick Snyder says news coverage about search warrants being used to get his state-issued cellphone and computer from government storage in the Flint water investigation is "very sloppy and misleading."

Prosecutors who dropped charges against eight people in the Flint water scandal explained their decision in a public forum Friday night, telling frustrated, shocked and saddened residents they must look at hundreds of mobile devices and millions of documents that a previous investigative team never reviewed.

A former FBI agent who investigated the Flint water scandal says the team was moving toward new charges when new prosecutors took over.

The Flint Water Crisis captured national attention about the dangers of lead contamination in public water supplies. Now, nearly six years after the problem was first detected, health officials are reporting on the well-being of children in Flint.

Let’s pick someone who doesn’t keep us awake nights.

Global real residential property prices rose 1.4% year/year in aggregate in Q3 2019, reflecting subdued developments both in advanced (+ 1.5%) and emerging market economies (+ 1.3%).

Inflation pressure could be weak even after consumer demand for non-discretionary goods and services begins to grow as the economy gets back on its feet.

Gold and silver prices often move in tandem, but the gap between them widened by 31% January through April in line with a trend that began nine years ago.

Speech by Mr Fehmi Mehmeti, Governor of the Central Bank of the Republic of Kosovo, at the press conference where the details of the measures taken by the CBK for maintaining health in the economy were given, Pristina, 3 April 2020.

Interview with Mr Luis de Guindos, Vice-President of the European Central Bank, and Expresso, conducted by Mr João Silvestre on 15 April 2020.

Schools in western Quebec are getting ready to welcome students back next week, but things will look a lot different — and classrooms will likely be far from full. 

Todd Colvin, Ambrosino Brothers

Larry Shover, Efficient Advisors

Bob Iaccino, Path Trading Partners

Dan Deming, KKM Financial

Todd Colvin, Ambrosino Brothers