The new service will join others at the existing 811 HealthLine, helping connect people with their local food services and offering answers for those without access to online services.

Independent MHA Eddie Joyce is suing four people, including the premier, for defamation over the handling of a harassment complaint that saw him turfed from the Liberal cabinet and caucus in 2018.

Long-term care homes in Eastern Health's jurisdiction are allowing for people to again visit their relatives by windows, just in time for Mother's Day. 

The province is still on track to move to a lower alert level Monday, which would ease some of the public health measures in place aimed at curbing COVID-19.

There are 14 active cases of COVID-19 remaining in the province.

Buenos Aires, Argentina :: Logos Hope's crew joins Youth With A Mission to show God's love to elderly people.

An OM worker in Cambodia shares about how a new training she is attending is transforming the way she does ministry.

The Art Zone is a place where teens can creatively express themselves and their worship for God. For Saskia, art is a way to quiet her mind and let God speak.

Mar del Plata, Argentina :: Crew work alongside a pastor who has plans to improve children's futures.

People and businesses are rallying to support the Muslim community in Fort McMurray recover after widespread flooding that damaged many neighbourhoods.

Residents are cleaning up damage to hundreds of homes and businesses caused by floods in the northern Alberta hamlet of Fort Vermilion.

The NDP health critic says comments Premier Doug Ford made this week calling out medical officers of health for not getting enough COVID-19 testing done are uncalled for.

Tensions are rising on Manitoulin Island because a First Nation is stopping travellers on provincial highways that go through the community. But opinions on M'Chigeeng's attempt to protect its people from COVID-19 are not divided along racial lines. 

Three residents at a long-term care facility in Sault Ste. Marie have tested positive for COVID-19, prompting officials to declare an outbreak at Extendicare Maple View.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

The Porcupine Health Unit is declaring a COVID-19 outbreak at a long-term care home in Iroquois Falls.

Provincial surveillance testing has returned a positive case of COVID-19 in a resident of Wikwemikong Nursing Home on Manitoulin Island. Ogimaa Duke Peltier says every staff member and resident underwent tests Tuesday and Wednesday of this week and the results are starting to come in.

The following is a post by Kristi Finefield, Reference Specialist in the Prints & Photographs Division, and member of the Picture This blog team. Images have a way of opening our eyes to new aspects of a well-known story. When I think of singer Marian Anderson, an image of her performing at the Lincoln Memorial […]

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

Over many years, the Xenopus laevis embryo has provided a powerful model system to investigate how mechanical forces regulate cellular function. Here, we describe a system to apply reproducible tensile and compressive force to X. laevis animal cap tissue explants and to simultaneously assess cellular behavior using live confocal imaging.

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H₂O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

This paper reviews post-crisis financial regulatory reforms, examines how they fit together and identifies open issues. Specifically, it takes stock of the salient new features of bank and CCP international standards within a unified analytical framework.

Even celebrity chef Julia Child said that the sleek appliance made mixing 'marvelous'

The graphic designer is receiving a Lifetime Achievement Award from Cooper Hewitt for her recognizable computer icons, typefaces and graphics

Press release: CPMI report sets out considerations for developers of wholesale digital tokens, 12 December 2019

Press release: Basel Committee invites comments on the design of a prudential treatment for crypto-assets, 12 December 2019.

The Bank of Canada, the Bank of England, the Bank of Japan, the European Central Bank, the Sveriges Riksbank and the Swiss National Bank, together with the Bank for International Settlements (BIS), have created a group to share experiences as they assess the potential case for central bank digital currency (CBDC) in their home jurisdictions. (Press release, 21 January 2020)

Markets Committee calls for wider adoption of global code of conduct for foreign exchange markets (Press release, 30 January 2020)

BCBS Press release "Basel Committee and IOSCO announce deferral of final implementation phases of the margin requirements for non-centrally cleared derivatives", 3 April 2020

CPMI Press release "Jurisdictions move towards full implementation of standards for financial market infrastructures", 8 April 2020.

Press release "Saudi G20 Presidency and the Bank for International Settlements (BIS) Innovation Hub invite global innovators to solve RegTech and SupTech challenges", 27 April 2020

BCBS Press release "Basel Committee issues progress report on banks' implementation of the 'Principles for effective risk data aggregation and reporting'", 29 April 2020

Rutgers' RUCDR Infinite Biologics received an amended emergency use authorization from the FDA late Thursday for the first SARS-CoV-2 coronavirus test that will allow people to collect their own saliva at home and send to a lab for results.

A randomized, controlled clinical trial evaluating the safety and efficacy of a treatment regimen of the investigational antiviral remdesivir plus the anti-inflammatory drug baricitinib for coronavirus disease 2019 (COVID-19) has begun.

Will a nationalist president be undone by his underreaction to a foreign threat?

When the province eventually conducts its review on the response to COVID-19, the president of the Nova Scotia Nurses’ Union can think of one thing in particular that needs to be discussed.

People in Truro, N.S., are reeling after the disappearance of three-year-old Dylan Ehler as the community tries to come to grips with another potential tragedy in the area.

Some school travel groups in Cape Breton that had trips cancelled in March due to COVID-19 are still waiting to get their money back.

Police say efforts to find the driver of an abandoned car in Kings County last week likely saved his life.

As Nova Scotians gazed up at the Canadian Forces Snowbirds soaring through the clouds last weekend, Matt MacKenzie was looking down and feeling the weight of successive tragedies that had enveloped his home province. 

For those working in CHSLDs — the province's long-term care homes which are the site of the most serious outbreaks — the bonus will amount to as much as $1,000 per month.

Quebec's public health director, Dr. Horacio Arruda, says grandparents under 70 years old are allowed to see and even care for their grandchildren. But the recommendation comes with cautions.

Jean-Talon market has changed during the COVID-19 pandemic. Today, there are controlled entrances, someone making sure you douse your hands with sanitizer and another with a clicker in hand, counting the number of people who enter.

Sheltering in place doesn’t mean you have to give up the best of wine country's offerings

The COVID-19 pandemic has created a surge in nationwide shipments of specialities from legendary restaurants

The Los Angeles Dodgers are being sued for negligence by a man who was attacked in the parking lot of Dodger Stadium and left with brain damage, his lawyers announced Friday.