The Amateur Traveler talks to Erik Smith about his month long visit to New Zealand. We focus in this interview about the South Island of New Zealand. Erick started in Christchurch and then visited The Banks Penninsula, Lake Tekapo, the Dunedin, the Otago Peninsula, the Southern Scenic Route including the Catlins, Fiordland National Park (including cruising both Doubtful Sound and Milford Sound) Queenstown, the rainy west coast, Abel Tasman National Park, Kaikoura, and Picton.

The Amateur Traveler talks to Lee Abbamonte, the youngest American ever to travel to every country in the world, about 3 islands off the coast of Australia: Lord Howe Island, Norfolk Island, Christmas Island.

The Amateur Traveler talks to Jim Lutz from Via Adventures about the Ecuador's Galapagos Islands. "The Galapagos has to be one of the most unique and wonderful wildlife destinations in the world."

Hear about Queensland's Sunshine Coast north of Brisbane Australia from Annabel Candy. Long white sand beachs, hiking trails, a tropical zoo in the tropics.

Hear about travel to Slovenia as the Amateur Traveler talks again to Michael Soncina about his visit to the country. The country is independent, beautiful and deserves to see more tourists than it gets.

Hear about travel to the Isle of Man as the Amateur Traveler talks to writer and film maker Andrew White. The Isle of Man is a small island in between Ireland and England. "It's a fantastic place. It really is. It's often said it's like the British Isles in miniature."

Hear about travel to the Windward Islands of the Lesser Antilles in the Caribbean as the Amateur Traveler  talks again to Gary Arndt of Everything-Everyhwere.com. Gary just finished an island hopping tour that took him to most of the islands in the Caribbean. We will cover the islands of St. Martin, Anguilla, Saba, St. Barts, St. Eustatius, St. Kitts, Nevis, Antigua, Barbuda, and Montserrat on this episode.

Hear about travel to the Island of Shikoku, Japan as the Amateur Traveler talks to Dan from Chicago about his recent trip back to the island. Dan also worked teaching English on Shikoku in the city of Imabari. Shikoku is the 4th largest of the islands in Japan and just across the from Hiroshima. Shikoku is mountainous, more traditional and more rural than the areas of Japan more tourists know.

Hear about travel to the Windward Islands of the Lesser Antilles in the Caribbean as the Amateur Traveler talks to Gary Arndt about his journey to visit many of the islands in the Caribbean on a single trip. In this episode we talk about Dominica, Martinique, Saint Lucia, Saint Vincent and the Grenadines, Grenada, Barbados, and Trinidad. This episode is a continuation of episode 386 which talked about the Leeward Islands.

Hear about travel to the Island of Socotra and Yemen as the Amateur Traveler talks to Earl from WanderingEarl.com about a visit to this isolated island best known for its production of frankincense. The island is quite isolated and a third of its plant life is found nowhere else on the planet. With trees like the umbrella-shaped dragon’s blood tree it has been described as the most alien-looking place on Earth.

Hear about travel to Indonesia as the Amateur Traveler talks to repeat guest Chris Willis about his trip to Indonesia. The main objective of Chris’s trip was to photograph Komodo dragons in the wild. Komodo dragons live on 2-3 islands which are not near any airport so getting there is half the experience.

Hear about travel to Slovakia as the Amateur Traveler talks to Julie Callahan from TheWorldInBetween.com. When Julie's family moved to Bratislava, Slovakia 3 years ago they didn't have a single friend who knew where this central European country was located. 

Hear about travel to the island of Madeira as the Amateur Traveler talks to Jörgen Abrahamsson who is a tour guide from Tue Nordic in this small Portuguese possession.

Hear about hiking the Kings Trail (Kungsleden) in Swedish Lapland as the Amateur Traveler talks to Agata from nullnfull.com about this northern and rugged portion of Sweden. Agata says, “Lapland is all about nature and wilderness”.

Hear about travel to the region around Island of Cebu as the Amateur Traveler talks to Tommo & Megsy from fivedollartraveller.com about their vacation in Visayas, the central group of islands in the Philippines. They visited the islands of Bohol, Cebu and Negros. 

Hear about volunteer travel with the Peace Corps as the Amateur Traveler talks to Peace Corps Director Carrie Hessler-Radelet. Director Hessler-Radelet not only served in the Peace Corps herself in Western Samoa but 4 generations of her family have done so. 

Hear about travel to the Cayman Islands as the Amateur Traveler talks to Kendra Pierre-Louis from kendrawrites.com about her trip to this island paradise.

Hear about travel to Michigan's Upper Peninsula (the UP)  as the Amateur Traveler talks to Jennifer Schuitema from Grand Rapids Michigan about travel to the northern remote part of the Great Lake State.

Hear about travel to the Aeolian Islands as the Amateur Traveler talks to Rick Zullo from rickzullo.com about these historic but lesser known islands off the coast of Sicily.

Hear about travel to the Island of Kauai as the Amateur Traveler talks to Betsy and Pete Wuebker from PassingThru.com about the Garden Island. 

Hear about travel to the "Sea Islands" off the coats of Georgia and South Carolina as the Amateur Traveler talks to Ruby & Peter from ajourneywelove.com about their travels in this archipelago.

Hear about travel to the Lofoten Islands of Norway as the Amateur Traveler talks to David Nikel, author of the Moon guide to Norway, about one of his favorite regions in that beautiful country.

Hear about travel to Providence, Rhode Island as the Amateur Traveler talks to Tamara Gruber from we3travel.com and the Vacation Mavens podcast about travel other home town.

Hear about travel to the Island of Crete in Greece as the Amateur Traveler talks to Elizabeth from compassandfork.com about her travels to this special Greek Island.

Hear about travel to the island of Sumatra in Indonesia as the Amateur Traveler talks to Luke Mackin from wildsumatra.com about this far-flung destination he now calls home.

Hear about travel to the U.S. and British Virgin Islands as the Amateur Traveler talks to Captain Shane McClellan about a sailing trip to the area.

Hear about travel to the Big Island of Hawaii as the Amateur Traveler talks to Justin Barnes from justinandcrystal.com about his island home.

Hear about travel to The Island of Samos in Greece and the vicinity as the Amateur Traveler talks to Dan from the Zipping Around the World podcast about this part of the Greek Islands. 

Hear about travel to Slovenia as Chris Christensen (the Amateur Traveler) talks about his recent solo road trip through western Slovenia as a guest of the Slovenia Tourism board. 

Hear about travel to the Canary Islands as the Amateur Traveler talks to Alice Ford from alicesadventuresonearth.com about the best of the Canary Islands.

A powerful player on Facebook’s new content oversight board infamously mocked Barron Trump during last year’s impeachment hearings, sowing doubt in the social media platform’s promises of unbiased moderation. The selection of Pamela Karlan, a professor at Stanford Law School, was announced Wednesday by Facebook, according to CNBC. Karlan will sit alongside 19 other experts…

The post Academic Who Infamously Mocked Barron Trump Gets Censorship Gig from Facebook appeared first on The Western Journal.

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Achieving a good night’s sleep isn’t always easy. Busy schedules, frequent stress, and bad rest environments can be culprits for sleep troubles. And it’s more common than you think. 

According to the American Sleep Association, 50 to 70 million U.S. adults have a sleep disorder. If your sleepless nights are taking a toll on your health, it might be time for you to buy a new pillow. 

RCMP say a 50-year-old man died and a 60-year-old man is in hospital following a crash involving a CP Rail train Friday morning.

Danny Jordaan says it would be massively complicated to ensure the safety of supporters should the PSL return while Covid-19 is still prevalent in South Africa.

Sundowns coach Pitso Mosimane says the premature return of football, even behind closed doors, poses a number of potential problems.

Signs of Slowing Unemployment

Where is Unemployment Slowing Faster During the...

Signs of Slowing Unemployment

Decision-making can be tough especially when the best choice isn’t glaringly obvious. We’re faced with this process every minute of the day. When do I wake up? What’s for lunch? Do I want to go out on a date with this person? What do I want to do with my life? Can I stay in […]

The post Stop Losing Sleep: 7 Steps for Stress-free Decision-making appeared first on Dumb Little Man.

The DML News App offers the best in news reporting.

The post REPORT: US slams new restrictions on journalists from this country appeared first on Dennis Michael Lynch.

While infection rates remain steady across the region, the Simcoe Muskoka District Health Unit is reporting more than half of all 360 cases have now recovered.

The following article, Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative, was first published on 100PercentFedUp.com.

TDS or Trump Derangement Syndrome brings out the worst in a lot of people. One young Texan girl’s story of her intolerant parents who refuse to attend her wedding because she’s marrying a conservative, however, is a new low. Lawyer and conservative civil rights activist, Rogan O’Handley tweeted a Tik Tok video of a Texan […]

Continue reading: Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative ...

The following article, Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant”, was first published on 100PercentFedUp.com.

Warren Norred, attorney for Shelley Luther, joined Tucker Carlson to discuss the decision by an Obama loyalist Texas judge to jail his client for reopening her salon during the COVID-19 pandemic. Norred said the judge has exposed himself for the “tiny tyrant” that he is. The judge jailed Luther and even fined her $7,000. Texas […]

Continue reading: Attorney for Jailed Dallas Salon Owner Shelley Luther Slams Obama Loyalist Judge as a “Tiny Tyrant” ...

The Mycobacterium tuberculosis virulence factor EsxA and its chaperone EsxB are secreted as a heterodimer (EsxA:B) and are crucial for mycobacterial escape from phagosomes and cytosolic translocation. Current findings support the idea that for EsxA to interact with host membranes, EsxA must dissociate from EsxB at low pH. However, the molecular mechanism by which the EsxA:B heterodimer separates is not clear. In the present study, using liposome-leakage and cytotoxicity assays, LC-MS/MS–based proteomics, and CCF-4 FRET analysis, we obtained evidence that the Nα-acetylation of the Thr-2 residue on EsxA, a post-translational modification that is present in mycobacteria but absent in Escherichia coli, is required for the EsxA:B separation. Substitutions at Thr-2 that precluded Nα-acetylation inhibited the heterodimer separation and hence prevented EsxA from interacting with the host membrane, resulting in attenuated mycobacterial cytosolic translocation and virulence. Molecular dynamics simulations revealed that at low pH, the Nα-acetylated Thr-2 makes direct and frequent “bind-and-release” contacts with EsxB, which generates a force that pulls EsxB away from EsxA. In summary, our findings provide evidence that the Nα-acetylation at Thr-2 of EsxA facilitates dissociation of the EsxA:B heterodimer required for EsxA membrane permeabilization and mycobacterial cytosolic translocation and virulence.

1 September 2016 , Volume 92, Number 5

Yue Chen
May 1, 2007; 6:812-819
Research

Incorporation of ribonucleotides into DNA can severely diminish genome integrity. However, how ribonucleotides instigate DNA damage is poorly understood. In DNA, they can promote replication stress and genomic instability and have been implicated in several diseases. We report here the impact of the ribonucleotide rATP and of its naturally occurring damaged analog 1,N6-ethenoadenosine (1,N6-ϵrA) on translesion synthesis (TLS), mediated by human DNA polymerase η (hpol η), and on RNase H2–mediated incision. Mass spectral analysis revealed that 1,N6-ϵrA in DNA generates extensive frameshifts during TLS, which can lead to genomic instability. Moreover, steady-state kinetic analysis of the TLS process indicated that deoxypurines (i.e. dATP and dGTP) are inserted predominantly opposite 1,N6-ϵrA. We also show that hpol η acts as a reverse transcriptase in the presence of damaged ribonucleotide 1,N6-ϵrA but has poor RNA primer extension activities. Steady-state kinetic analysis of reverse transcription and RNA primer extension showed that hpol η favors the addition of dATP and dGTP opposite 1,N6-ϵrA. We also found that RNase H2 recognizes 1,N6-ϵrA but has limited incision activity across from this lesion, which can lead to the persistence of this detrimental DNA adduct. We conclude that the damaged and unrepaired ribonucleotide 1,N6-ϵrA in DNA exhibits mutagenic potential and can also alter the reading frame in an mRNA transcript because 1,N6-ϵrA is incompletely incised by RNase H2.

7 May 2020 , Volume 96, Number 3

3 June 2013 , Volume 69, Number 3

Dark creations of European authors tell us much about our own world