The present disclosure relates to the technical field of degradation of persistent pollutants and discloses a method for efficiently degrading a perfluorinated compound (PFC), through which the problems of harsh reaction conditions and less high defluorination rate existing in prior-art methods for degrading PFCs are solved. In the present disclosure, a 3-indoleacetic acid (IAA) solution is irradiated with 254 nm UV light to generate hydrated electrons, with which the PFC are degraded by reduction under an aerobic condition, where an organo-modified montmorillonite is added to provide a reaction microzone, so the degradation and defluorination effects of the hydrated electrons for the PFC are greatly improved. The method for degrading a PFC according to the present disclosure is not affected by the pH of and the dissolved oxygen in the solution and less affected by the humic substances in a water body, thereby overcoming the defects in existing methods for degrading PFCs with hydrated electrons while the degradation efficiency is ensured. Therefore, the present disclosure is of great application value.

A method for treating contaminated liquids in a flow by ozone, wherein the ozone is fed in gas form into the flow at one point and then is mixed into the flow in stages by mixers following one another in the flow direction, so that an absolute quantity of introduced ozone increases after each mixer until a feed efficiency of more than 95 percent is reached.

Disclosed herein are methods for reducing fouling caused by process water present within a water recycling loop of a pyrolysis plant. The process water includes water, a pygas, and in some cases pygas byproducts. Fouling is caused by phase separation and accumulation of materials from the process water on equipment surfaces. The method includes applying a total of about 5 ppm to 500 ppm of one or more antifouling polymers to the process water to form a treated process water. The one or more antifouling polymers are selected from the group consisting of copolymers of unsaturated fatty acids, primary diamines, and acrylic acid; copolymers of methacrylamidopropyl trimethylammonium chloride with acrylic acid and/or acrylamide; copolymers of ethylene glycol and propylene glycol; and blends of two or more thereof.

A filter for filtering pyrolysis oil and an arrangement. The filter is a split-flow filter (1, 1a, 1b), comprising a filter element (12), comprising plurality of apertures (17) extending through the filter element (12), a receiving channel (13) for receiving a flow (F) of pyrolysis oil to be filtered and for supplying said flow on first side (A) of the filter element (12), a main discharge channel (14) arranged on the first side (A) of the filter element (12) for discharging the portion of the flow (F) of pyrolysis oil having not penetrated through the filter element (12), and a filtrate channel (15) arranged on second side (B) of the filter element (12) for discharging the portion of the flow (F) of pyrolysis oil having penetrated through the filter element (12).

Plant for melting and cleaning of snow and ice, distinguished in that the plant comprises a means for melting using enthalpy of a water source in order to melt snow and ice, and a means for cleaning for cleaning out the pollution from the water phase that contains the melted snow and ice. Method for melting and cleaning of snow and ice, using the plant according to the invention.

5 A liquid cleaning system rests on a surface having a non-porous material covering and a drain. A lower plate layer and an upper plate layer each have runners arranged in a grid. The lower plate layer rests upon the surface and the upper plate layer rests upon the lower plate layer offset at an angle with respect to the grid of the lower plate layer. An upper layer covers the upper plate layer and has a plurality of holes for the passage of fluids into the liquid cleaning system. As liquid enters the liquid cleaning system through the holes, the liquid traverses the grid of the lower plate layer and the grid of the upper plate layer flowing towards the drain, and contaminants within the liquid collect within the grid of the lower plate layer and the grid of the upper plate layer.

Roughly fifty demonstrators marched from Fruitvale BART on Friday night to Oakland City Hall in an anti-police rally called in response to the fatal shooting of Richard Perkins by Oakland police on November 15. But unlike protests earlier this year, police didn’t try to drive demonstrators onto the sidewalks to comply with Oakland Mayor Libby Schaaf’s policy banning nighttime street marches.…

First, a loud siren seared my ears. When I turned, I saw four men draped in bandages standing atop a white hearse.…

'Don’t build technology for Mordor.' On Saturday at Peter Thiel’s San Francisco home overlooking the Bay, protesters demonstrated against a collaboration between Immigration and Customs Enforcement and Palantir Technologies, a data-mining firm that Thiel co-founded. As reported in The Intercept, ICE will be using a new Palantir product called Investigative Case Management to to aid in its expanded deportation mission.…

ACT for America, which the Southern Poverty Law Center has designated as an anti-muslim hate group, showed up on Saturday morning in cities nationwide, dubbing its protest a "March Against Sharia." Berkeley was spared, but the group appeared in nearby Santa Clara, and in Roseville, a suburb of Sacramento.…

The organization sets up at 27th Street and Northgate Avenue and at 6th and Castro streets. Blair Hippolyte and Jannie Rutledge, two Oakland residents who are homeless, sat on the sidewalk of 27th Street.…

Oakland-based Cream Co. Meats announced recently that, for the first time ever, it will start delivering its products on a retail basis. Statewide Covid-19-related shelter-in-place regulations mandated the closure of many restaurants and other businesses that regularly serve and sell Cream Co. products.…

Embodiments of the disclosure encompass methods of amplifying nucleic acid from one or more cells using MALBAC (multiple annealing and looping-based amplification cycles) primers. In particular embodiments, the nucleic acid is amplified as amplicons in a linear manner. Specific embodiments include the removal or effective destruction of nonlinearly produced amplicons.

Provided herein are methods of reliably determining whether or not an individual is a user of tobacco.

The invention concerns nucleic acids coding for mutated or truncated forms of the human parkin gene, or forms comprising multiplication of exons, and the corresponding proteins and antibodies. The invention also concerns methods and kits for identifying mutations of the parkin gene, and for studying compounds for therapeutic purposes.

The present invention relates to methods for predicting the survival time of patients suffering from cancer. Said methods are based on the quantification and analysis of the cell free nucleic acids that are present in a sample from the patient and typically include the determination of the level of the mutant nucleic acid which contains a mutation of interest, the calculation of the mutation load for said mutation of interest, the calculation of the DNA integrity index or a combination thereof.

Disclosed are methods and compositions for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (RT-PCR) and multiplex polymerase chain reaction techniques as well as Template Exchange Extension Reaction (TEER) to detect the presence of point mutations, truncations, or fusions of anaplastic lymphoma kinase.

A method of detecting a cancer progression observation index gene group, comprising: a) preparing a plurality of AKR/J mice in which an oncogene is inserted into a thymocyte; b) dividing the AKR/J mice into three groups, raising the first group of the AKR/J mice after high-dose ionizing radiation of 0.8 Gy/min, raising the second group of the AKR/J mice after low-dose ionizing radiation of 0.7 mGy/hr, and raising the third group of the AKR/J mice in a general environment; c) obtaining a thymus of a dead mouse of the first or second group of the AKR/J mice throughout the b) operation and diagnosing cancer when a weight of the thymus is increased to twice or more than before radiation; d) extracting thymuses by sacrificing the first to third groups of the AKR/J mice at the time at which the third group of the AKR/J mice initially die; and e) selecting only a thymus having no change in weight compared to the organs of the same-aged non-irradiated AKR/J mice from the organs extracted in d) and detecting a gene of the organ whose weight is increased or decreased by a factor of two or more through gene analysis.

Compositions and methods for detecting Trichomonas vaginalis are provided.

In some embodiments, the present disclosure pertains to a method for retrieving at least one molecular recognition element in a fixed tissue. In some embodiments the method comprises preparing a solution comprising at least one aldehyde-scavenging agent. In some embodiments, the method comprises contacting the fixed tissue with the solution. In some embodiments, the tissue is fixed with an aldehyde-based cross-linking agent. In some embodiments, a reaction of the aldehyde-scavenging agent with the aldehydes comprising the cross-linking agent retrieves the at least one molecular recognition t. In some embodiments, the at least one rolecular recognition element comprises of amino acids, peptides, proteins, nucleic acids, carbohydrates, lipids, or a combination thereof. In some embodiments, the at least one aldehyde-scavenging agent comprises of beta-dicarbonyl compounds, mono or di-amide scavengers, ethyl alcohols, sulfur containing compounds, mercaptoethylamines, mercaptoethanols, hydrazines, ethanolamines, hydroxylamines, anilines, variation of amines, activated charcoal, phenols, or mixtures and combinations thereof.

The present disclosure relates to use of high-concentration aldehyde-based fixatives to fix tissue samples. Aldehyde concentrations are selected that significantly increase rate of diffusion of the fixative solution into the tissue. When combined in the cold-temperature step of a two-temperature fixation, a substantial improvement in the preservation of post-translationally modified proteins is achieved.

In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.

Aspects of the present disclosure include methods for processing a biological sample. Methods according to certain embodiments include contacting a biological sample having cells with an assay reagent that includes one or more analyte-specific binding members to produce a biological sample assay composition and introducing the biological assay composition into an inlet of a flow cytometer having an integrated acoustic separator. Systems, including a flow cytometer with integrated acoustic separator having one or more acoustic concentrator devices suitable for practicing the subject methods are also described.

The invention is drawn to novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors, conduits for fluids, and electronic conductors. The nanostructures can be used in microfluidic devices, for transporting fluids between devices and structures in analytical devices, for conducting electrical currents between devices and structure in analytical devices, and for conducting electrical currents between biological molecules and electronic devices, such as bio-microchips.

The present invention provides methods of determining cell sensitivity to a therapeutic agent.

Light harvesting luminescent multichromophores that are configured upon excitation to transfer energy to, and amplify the emission from, an acceptor signaling chromophore in energy-receiving proximity therewith are provided. Also provided are compositions for labelling a target. The labelling composition may include a donor light harvesting multichromophore and an acceptor signaling chromophore in energy-receiving proximity to the donor light harvesting multichromophore. Also provided is an aqueous composition for labelling a target, including: a donor light harvesting multichromophore; an acceptor signaling chromophore in energy-receiving proximity therewith; and a sensor biomolecule. Methods for using the subject compositions are also provided.

The present invention relates to a novel method for detecting a detection object in a sample, and a detection device using the same. The detecting method of the present invention uses a “bridge composite” in which gold nanoparticles and an antibody specific to a detection object are coupled in order to induce a sufficient coupling reaction between the antibody and the detection object, thereby improving reactivity. Accordingly, since excellent resolution is provided, the method of the present invention has advantages of enabling accurate concentration measurement of a detection object in a sample, and amplifying a measurement signal. In addition, the method of the present invention can effectively detect small molecules such as hormones, vitamins, etc. having a small molecular weight.

Methods of screening for expression of an RNA associated with a post-kidney transplant complication include collecting vesicles from urine, isolating vesicle-associated RNA, and analyzing expression patterns. In particular, AIF1, BTN3A3, CCL5, CD48, HAVCR1, or SLC6A6 mRNA expression patterns are analyzed.

Methods and compositions are described for categorizing and treating autoimmune disease, using single cell network profiling (SCNP), where activation levels of one or more activatable elements are determined in single cells, with or without modulation, to categorize or determine treatment for the autoimmune disease.

The present invention relates to in vitro methods of assessing the susceptibility or responsiveness of a subject to the treatment with an epidermal growth factor receptor (EGFR) inhibitor/antagonist, wherein the subject has been diagnosed or suspected of suffering from inflammation-associated. These methods comprise determining the level of expression of EGFR in myeloid cells in a sample from the subject, wherein an expression of EGFR in the myeloid cells is indicative of the subject being susceptible to the treatment with an EGFR inhibitor/antagonist. The invention also relates to EGFR inhibitors/antagonists for use in the treatment or amelioration of inflammation-associated cancer. The invention furthermore provides in vitro methods of prognosing the survival time, progression-free survival time or disease course of a subject that has been diagnosed or suspected of suffering from from inflammation-associated cancer. In addition thereto, the invention relates to in vitro diagnostic methods of assessing the proneness of a subject to develop inflammation-associated cancer in which the expression of EGFR is determined in myeloid cells from the subject.

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

The present invention relates to an in vitro method of diagnosing chronic myelomonocytic leukemia (CMML) in a subject, said method comprising the steps of: a) Detecting a monocyte population in a biological sample from said subject; b) Quantifying the CD14+/CD16− monocytes in said biological sample; c) Comparing the value of step b) to a reference value; and d) Diagnosing CMML based on said comparison. Preferably, said detecting step a) is performed by an exclusion gating strategy by flow cytometry.

A method to detect prostate cancer comprising contacting a sample of prostate cells from the patient with a set of detectably labeled probes under hybridization conditions and determining the presence of chromosomal abnormalities in prostate tumor tissue, PIN (intra-epithelial neoplasia), histologically benign tissue and benign prostatic hyperplasia (BPH); a method to combine immunofluorescence and FISH (IF-FISH) to facilitate the assessment of chromosomal abnormalities; a set of probes; and a kit comprising the set of probes and instructions for diagnosing prostate cancer in a patient.

We have discovered a protein in humans, herein referred to as collagen like gene (CLG) product (SEQ ID NOS: 12 and 13), that is expressed in human prostate cancer and breast cancer cell lines but not in normal adult, placenta, lung, liver, skeletal muscle, kidney or pancreas tissues. We have also discovered that the level of CLG mRNA expression correlates positively with the metastatic potential of the cancer cell lines tested.

The present invention provides detection methods for detecting a pre-cancerous epithelial cell signature. The present invention further provides reagents for use in the detection methods. A subject detection method is useful in various imaging, diagnostic, prognostic, and patient monitoring methods, which are also provided.

The diagnosis of male infertility is based predominantly on the results of standard semen analysis for concentration, total motility, progressive motility, volume, pH, viscosity and/or morphology. When sperm enter the female reproductive tract, they must undergo a series of physiological changes, known as capacitation, in order to fertilize an egg. This process involves plasma membrane changes that occur in response to stimuli within the female tract. These changes include removal of sterols and redistribution of the ganglioside GM1. Semen analysis identifies only half the cases of male infertility due to standard semen analysis providing little information on sperm functional competence. Previous data demonstrated that localization of the ganglioside, GM1, identifies sub-populations of sperm capable of undergoing the functional maturation process known as capacitation and tracks strongly with fertility.

Methods for multiplexed delivery of agents to a solid tissue in vivo followed by assessment of efficacy with mass spectrometry are described.

A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers.

The present invention relates to methods of determining if a subject has an increased risk of suffering from memory impairment. The methods comprise analyzing at least one sample from the subject to determine a value of the subject's exosomal profile or combined biomarker profile (lipids plus exosomal cargo) and comparing the value of the subject's exosomal or combined biomarker profile with the value of a normal exosomal or biomarker profile, respectively. A change in the value of the subject's exosomal or combined biomarker profile, including a change in the subject's exosomal or combined biomarker profile, over normal values is indicative that the subject has an increased risk of suffering from memory impairment compared to a normal individual.

In sporadic Alzheimer's disease, neurofibrillary lesion formation is preceded by extensive post-translational modification of the microtubule associated protein tau. Immunoassays have been developed recently that detect tau in biological specimens, thus providing a means for pre-mortem diagnosis of Alzheimer's disease, which has remained elusive. These assays have been improved by the analysis of relevant post-translational modifications, such as phosphorylation, however opportunity for improvement remains. The present invention addresses this issue by disclosing synthetic methylated peptides derived from the tau protein of paired helical filaments and non-diseased control brain. Alzheimer's disease specificity is provided by the presence or absence of methyl moieties on lysine residues and differences between mono-, di-, and tri-methylation. The methylated peptide is useful as an antigen and a binding partner for identifying compounds that interact with the peptide and the methylated tau protein, including antibodies that can distinguish non-diseased brain from that affected by Alzheimer's disease. The resulting antibodies are useful diagnostically and therapeutically. The compounds that specifically bind to methylated tau proteins are useful for eliminating abnormally methylated tau.

Provided are a detection agent for detecting hydroxy vitamin D, preparation method thereof, and use thereof in 25-hydroxy vitamin D immunological detection. The detection agent comprises a conjugate formed by a 25-hydroxy vitamin D antigen derivative and protein carrier, and magnetic spheres coated by the conjugate. Also provided is a 25-hydroxy vitamin D detection kit comprising the detection agent.

A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

Oakland's alaMar Kitchen & Bar is about to start serving 1,500 free meals per week to restaurant workers who have been displaced and derailed by the coronavirus outbreak.

Located at 100 Grand Ave., chef Nelson German's popular seafood restaurant has just issued this announcement:

"We are here for you, Oakland! ...…

Off the Grid will stage a two-part free-meal giveaway today, April 8, from 12 noon to 1 p.m. at Nob Hill Foods Alameda and from 2:30 p.m. to 3:30 p.m. at Pak 'N Save Emeryville.

It's part of the Grocery Employees Meal Fund, which OTG recently launched to help creators feed grocery-store employees and their family members throughout the Covd-19 crisis.…

The owners of Oakland's Homestead Restaurant have just debuted a new business concept that is meant to outlast the Covid-19 crisis.

Currently operating at the restaurant's 4029 Piedmont Ave. space Tuesdays through Saturdays from 10 a.m. to 6 p.m. via curbside pickup and Caviar or Doordash delivery, The Humble Sandwich comprises a selection of classic deli sandos made with the same high-quality ingredients for which the seven-year-old restaurant is revered.…

The owners of Oakland's Homestead Restaurant have debuted a new concept meant to outlast the COVID-19 crisis.

Now through May 5, healthcare workers and first responders can collect free "Thank You Meals" at any McDonald's location nationwide.

The promotion began on April 22.…

Stories you shouldn’t miss:

1. Alameda County Public Health Director Muntu Davis contends that gentrification should be examined in terms of health impacts because of the displacement and stress it causes for low-income residents, the Bay Area News Group$ reports.…

The city's 911 system can leave callers waiting for more than 120 seconds before an operator ever answers. Seconds of delay in an emergency can lead to death. As a result, 911 calls are supposed to be answered quickly.…

The present invention relates to a process for producing an electrolytic capacitor, the process comprising process steps i) ii): i) providing a capacitor body (1) that comprises an electrode body (2) of an electrode material,a dielectric (3) which at least partially covers the surface of this electrode material, anda solid electrolyte (4) at least comprising an electrically conductive material which at least partially covers the dielectric surface; ii) applying a primer solution e) to the capacitor body (1), followed by an application of a solution or dispersion a) comprising a conjugated polymer b) and a solvent or dispersant d), followed by an at least partial removal of the solvent or dispersant d) for the formation of the polymeric outer layer (5) that is formed onto the capacitor body (1); wherein the primer solution e) comprises at least one monofunctional amine and at least one carboxylic acid.