In the late 1300s, tea was introduced to Japan from mainland China, transported in delicate jars. Over the years, as drinking tea became a prized activity in Japan, so too did the jars in which it was stored

Henry Ford spent the majority of the war as a pacifist. By 1917, however, his state-of-the-art assembly line was churning out vital engine parts to feed the war machine.

A contemporary weapons expert is about to test a medieval war hammer on a steel breastplate from that era. The aim is to find out how much damage this fearsome instrument of death could deal.

The dominant bear in the swamp is fending off another challenge to his authority by an intruder. It's a clash of titans with mating rights on the line.

Highway 8, otherwise known as the Dempster Highway, will be closed to all traffic at 5 p.m. on Friday, according to the government of the Northwest Territories.

Yellowknife RCMP confirmed the death of the missing 22-year-old woman on Friday and charged 27-year-old Devon Larabie in connection with her death.

The N.W.T. and Nunavut Workers’ Safety and Compensation Commission says it filed multiple charges in the Nunavut Court of Justice under the Nunavut Safety Act on May 1.

The remote cabin could be the difference between life and death for travellers stuck on the territory’s long winter road. It was built to remember Stella Barnaby, who would have been 55 this Saturday.

Darin Isaac was elected on Wednesday as the new chief of the Selkirk First Nation in Yukon. Isaac also held the position for two terms from 2005 to 2011. He has also served as a councillor for three terms.

Schools were closed March 13 to reduce the risk of spread of the coronavirus, and there is no plan to reopen them by the end of the current school year in June.

It looks like New Brunswickers will see more snow Saturday with a snowfall warning issued for central and northern parts of the province. 

Canadians helped liberate the Netherlands 75 years ago this week.

A spring storm is set to slam parts of New Brunswick this weekend, bringing up to 30 cm of snow. 

Public Health has recorded no new cases of COVID-19 on Saturday, said a news release from the government. 

Closing arguments were presented at the trial of Phillip Lionel Levac on Friday at Regina Court of Queen's Bench.

Police used their conductive energy weapons four times during the arrest of a 31-year-old man who they say was smashing windows with a piece of rebar.

Two men are charged in connection with an illegal drug distribution operation after Winnipeg police seized more than $2.2 million worth of methamphetamine, cocaine and ecstasy.

The premier’s office confirmed the news in an email statement, blaming visibility issues under "very specific lighting conditions."

A virus that spreads fear and stigma, as well as disease. It’s the story of HIV/AIDS as well as COVID-19. The former executive director of AIDS Thunder Bay reflects on the similarities he sees between HIV 35 years ago, and the coronavirus now.

Police in Thunder Bay have arrested and charged a man from the northwestern Ontario city following an investigation into allegations of child luring.

The Northwoods League announced Thursday that its 2020 season will not begin on May 26, as originally scheduled, due to the COVID-19 pandemic.

The Thunder Bay District Health Unit (TBDHU) confirmed one new case of COVID-19 in its catchment area Thursday, bringing the total in the area to 77, with 65 of those listed as resolved.

It's been more than a week since there were any new or active cases of COVID-19 in the catchment area for the Northwestern Health Unit (NWHU), and staff can not focus increasing testing.

A 40-year-old man has been arrested and charged in connection with two break-ins at the Thunder Bay District Health unit offices, the Thunder Bay Police Service announced in a written release Friday.

<span class="topsy_trackback_comment"><span class="topsy_twitter_username"><span class="topsy_trackback_content">FCC Launches New Round of Audits of Radio Station EEO Performance http://bit.ly/anoP3q</span></span>

Job of The Week: CEO – Nordoff Robbins Music Therapy http://bit.ly/aFIMNM

Crombie REIT, which owns the Avalon Mall, is awaiting details before applying for government's commercial rent relief.

Newfoundland and Labrador's already fragile economy suffered another gut-punch in April, with Statistics Canada reporting Friday that 29,000 jobs were lost in April alone.

COVID-19 is not an equal opportunity pandemic. As Memorial president Vianne Timmons writes in this guest column, women are often in harm's way because of their work.

Doron's experience on Logos Hope shows him God's faithfulness and uncovers leadership abilities he is using today in a new role.

"Since my childhood, I have been anxious about missing out. I remember not wanting to sleep whenever I heard the adults chatting in the night. I wanted to be part of it all. Later on, in high school, I said “yes” to every event and outing, which ended up crashing so many times. I couldn’t choose. I wanted to be there to celebrate all the fun moments but also share all the tears in the low moments," says Ivy. "However, this lifestyle of being afraid of missing out could not continue when I joined missions. I have had to learn how to let go when I miss out on opportunities to create precious memories with family and friends in my home country."

Police are investigating a report of a suspicious package in north Edmonton.

Tensions are rising on Manitoulin Island because a First Nation is stopping travellers on provincial highways that go through the community. But opinions on M'Chigeeng's attempt to protect its people from COVID-19 are not divided along racial lines. 

A new study facilitated by the Workplace Safety and Insurance Board of Ontario states miners who were forced to inhale an aluminum powder were found to have a higher risk of Parkinson’s disease.

Provincial surveillance testing has returned a positive case of COVID-19 in a resident of Wikwemikong Nursing Home on Manitoulin Island. Ogimaa Duke Peltier says every staff member and resident underwent tests Tuesday and Wednesday of this week and the results are starting to come in.

The following is a guest post by Martha H. Kennedy, Curator of Popular & Applied Graphic Art, Prints & Photographs Division. During Will Eisner Week, March 1-7, 2020, the Library of Congress joins art schools, libraries, universities, and museums in a global celebration of this legendary creator’s contributions to the world of comic art and […]

The following is a guest post by Hanna Soltys, Reference Librarian, Prints & Photographs Division. The post was written with the help of Sara W. Duke, Curator of Popular and Applied Graphic Art.   While professional baseball’s Opening Day will take place at a later date, the spirit and excitement of the day still live […]

Spring has arrived. While some of us may have an opportunity to carefully step outside and view blossoms in our own neighborhood, others may not. Wherever you are, you can take a virtual stroll among the shoots and blossoms planted among the collections of the Prints & Photographs Division. Many images of gardens can be […]

Visitors to the 1900 Paris Exposition would have had the opportunity to view an extraordinary display of photographs, charts, publications and other items meant to demonstrate the progress and resilience of African Americans in the United States, only a few decades after the abolition of slavery. The materials were assembled by African American intellectuals Thomas J. […]

The following is a guest post by Katherine Blood, Curator of Fine Prints, Prints & Photographs Division. As the Library of Congress marks its 220th year of serving the nation, the publication of a new guide tells two stories: how staff have for decades worked with art professionals to build the collections and how by […]

Below is an interview with Elizabeth Lindqwister, the summer 2019 Liljenquist Family Fellow, and Prints & Photographs Division staff members, Karen Chittenden and Micah Messenheimer, about creating a Story Map focusing on the Civil War experience of Susie King Taylor. Many courageous people are pulling double and triple duty in this time of quarantine for […]

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.

The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.

Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

Mice, rats, or hamsters are immunized by giving biweekly injections of a purified antigen, cultured cells, or cDNA. For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50–100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100–200 µg is sufficient. Lower doses can be used for antigens with higher immunogenicity. Adjuvants (Freund's, Ribi, Hunter's TiterMax, ImmunEasy, or Alum) should be mixed with the immunizing antigen for the first two immunizations only; Complete Freund's adjuvant is only used with the first immunization. Subsequent immunizations are performed in phosphate-buffered saline (PBS) or normal saline, with or without Incomplete Freund's adjuvant. The choice of adjuvant is dependent on the subclass of immunoglobulin required. Over the course of the 6-wk immunization schedule, each animal usually receives a total of six injections (three subcutaneous and three intraperitoneal). Once a good titer has developed against the antigen of interest, regular boosts and bleeds are performed to collect the maximum amount of serum. For rats and hamsters, boosts should be spaced every 2–3 wk, and serum samples of 400–500 µL should be collected 10–12 d after each boost. For mice, boosts should be spaced every 2–3 wk, and serum samples of 200–300 µL should be collected 10–12 d after each boost.