Purifying and culturing cells from the central nervous system (CNS) has proved to be an incredibly powerful tool for dissecting fundamental neuron and glial properties, and especially powerful in understanding neuronal–glial interactions. In a series of detailed protocols, we have provided step-by-step instructions for purifying and culturing specific types of neurons, glia, and vascular cells from the CNS by immunopanning. This article discusses common pitfalls and errors as well as important design considerations for the immunopanning procedure.

SOLIDWORKS Image Quality settings have the biggest impact on file size and system performance. We always assumed that it would have an impact on overall performance also. The effect varies widely across different files, so we decided to do SOLIDWORKS

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2D design Despite the great popularity of spatial design programs and their unquestioned advantages, 2D design programs such as AutoCAD are still very popular and useful. One of such programs is DraftSight, which over the years has become very recognizable

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Documentation is always an asset for any of the company or industry. Documenting the right data always ensures the task or job, whatever assigned by the superiors in an industry. Whatever the design we do, at the end of the

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To me, one of the handiest SOLIDWORKS ease-of-use functions hides in plain view: The Command Search. Found at the top of the application, the SOLIDWORKS Search box can be set to several different types of search, but the one I

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This case study seeks to find the answer to a common question: Which way should you affix hooks to your fence so they can properly handle the load of a hanging flower basket? Javelin’s Application Engineer; Colin Murphy CSWE, takes

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This is the first blog post regarding the SOLIDWORKS activation error message. I will briefly try to go through the four most common activation error messages when using a standalone license. I will in a later blog post cover the

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All, As we’ve written before, Dassault Systèmes SOLIDWORKS is committed to helping our customers be the most productive while working from home during this time of social distancing. Right now, it’s not possible to grab a drawing from the plotter or

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Learn how to easily delete a face from a solid or surface body when modeling in SOLIDWORKS. Our #TechTip this week is by Training Manager John Setzer. Video Transcript Hi and welcome to another GSC tech tip. Today we’re going

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CNA Staff, Apr 21, 2020 / 02:19 pm (CNA).- Indigenous rights groups are warning that many families in Bangladesh who have lost jobs and income because of the coronavirus pandemic are not yet receiving aid, and many are facing starvation.

A joint statement from three Bangladeshi indigenous rights groups warns that the vast majority of those living in rural areas are day laborers, and are now out of work.

Bangladesh, a low lying county situated just east of India, has 3,400 confirmed cases of COVID-19 and has recorded 110 deaths. Officials say actual numbers are likely higher due to a lack of testing kits.

Romen Kisku, a Catholic father of five and member of the Santal ethnic group, told UCA News that his family has received a small amount of food aid from a charity, but he worries it will not be enough to feed his 10-member family. He and his family live in the northern region of Dinajpur, and he said he hopes to find work in a neighboring region as a paddy farmer.

“Three men of our family are day laborers and our income pays for everything. Now we cannot go to work and government aid has not reached us. The minimal aid was too little for our family, so we have to go on starving if things don’t improve,” Kisku told UCA News.

Ethnic-minority Santal people live mostly in northwestern Bangladesh as well as parts of India and Nepal. About eight percent are Christian.

Bangladesh has a low percentage of Catholics— as low as 0.3% in some areas— and the Catholic aid group Caritas is struggling to raise funds for the poor.

“We have made an appeal to people in our parishes and areas, so they come up with whatever they can to assist those having hard times during this crisis. The suffering of people will intensify if the crisis lingers, so we need to help people any way possible,” Bishop Sebastian Tudu of Dinajpur told UCA News.

Though the government and charitable organizations are mobilizing to provide relief, the remoteness of the areas where many indigenous people live mean that in some areas, very few families have yet received government assistance.

Though Catholic Relief Services is active in providing aid in refugee camps in Bangladesh, a CRS spokesperson confirmed to CNA that CRS is not currently active in the Dinajpur region.

The branch of Caritas in the southeastern port city of Chittagong has taken two emergency cash handouts of 2,250 taka (US$27) and 1,547 taka to support 950 families on the hills, UCA News reported.

Bangladesh’s government started food relief and subsidized food sales at the end of March, aiming to support about 50 million poor and needy people across the country, UCA News reports.

Bangladesh imposed a nationwide lockdown March 26, with a restriction of no more than five people allowed to congregate in prayer in a mosque, the New York Post reported. Despite this, on April 18 a crowd of some 100,000 people gathered for a funeral in Brahmanbaria, in the eastern part of the country.

Rome Newsroom, Apr 30, 2020 / 10:30 am (CNA).- The coronavirus pandemic is telling us that “our health counts more than the economy’s health and that true human fraternity is more valuable and noble than diplomatic success,” a Taiwanese archbishop has said.

Archbishop John Hung Shan-chuan of Taipei spoke to CNA about how the East Asian state has been at the forefront of efforts to halt COVID-19. 

Taiwan was one of the first countries to warn the international community about the risks of the pandemic, although its appeal was not heeded initially by the World Health Organization. 

The country, which has a population of 24 million, has had only 429 documented coronavirus cases and six deaths as of April 30, according to Johns Hopkins University Coronavirus Resource Center.

Archbishop Hung, who has overseen the archdiocese based in the Taiwanese capital since 2007, explained that the local Church has not faced a crisis because the government has been effective in countering the virus.

Taiwan also sent medical masks and supplies to the Vatican, to be delivered to the poor. Taiwan’s ambassador to the Holy See personally brought 280,000 medical masks to the Vatican and the Italian bishops’ conference, and donated food and supplies to the Papal Almoner.

Although Taipei has been considered a model in countering the pandemic, it has been overshadowed by its powerful neighbor, the People’s Republic of China, which claims Taiwan as part of its own territory.

This shadow also extends to Taiwan-Holy See relations. On September 18, 2018, the Holy See signed a confidential agreement with China on the appointment of bishops. The agreement is scheduled to expire in August, and negotiations for its renewal are underway.

Although the Holy See and Taiwan have had uninterrupted ties for almost 80 years, the Holy See is now keeping a low profile in relation to Taiwan, likely in order not to annoy mainland China.

The Holy See press office issued a statement April 9 thanking two Chinese foundations for providing medical supplies to the Vatican Pharmacy. Despite the considerable commitment of Taiwan, the Holy See has not released any official statement thanking Taiwan.  

The archbishop of Taipei, however, emphasized the broader picture and did not complain about the Vatican’s choice.

“The COVID-19 pandemic is an unprecedented global crisis and represents a danger to all humanity,” he said. “No nation, absolutely no nation, can extirpate it by operating alone without the help of other countries.”

“What the world badly needs now is solidarity in action, not self-interest. And it is good that China can provide material aid to other countries on a very large scale.” 

“Thanks to the donations of supplies from China, the Vatican will be able to help many, many poor people in other countries who are forgotten by the politicians and barely reported by the media.”

Archbishop Hung continued: “The Catholic Church in poor countries is waiting for help. And the Vatican will be thankful for any concrete gesture of solidarity, regardless of the political system of the countries as the Church is entrusted with the mission to proclaim the Good News to all nations promoting at the same time a culture of fraternity and peaceful co-existence.”

Turning to Taiwan, he said: “Thanks to the competence of the government authorities and the remarkable support of the population, the people in Taiwan need not deal with emergencies, such as cluster infection. Understandably, no special contribution in terms of material aid to the local population is solicited from Caritas Taiwan.”

But the archbishop highlighted the plight of migrant workers, who ran a high risk of contracting and spreading the coronavirus.

Caritas Taiwan has been very active, the archbishop reported, distributing face masks to fishermen and and-based migrant workers who would not have them otherwise because of government rationing of masks.

“Furthermore,” he said, “in collaboration with other NGOs, Caritas Taiwan also advocates for the protection of migrant workers who are vulnerable to COVID-19 and asked the government to let them overstay in Taiwan, in case their visa is due.”

Archbishop Hung applauded the government for taking preventive measures to counter the pandemic. He stressed that the local bishops’ conference reacted very quickly and “complied with the recommendations and regulations of the ministry of the interior and decided, one by one, to suspend all the indoor Church gatherings, including Mass celebration on weekdays and Sundays, to avoid at all cost any possible infection that could cause death and the closure of the church premises.”

The Catholic Church in Taiwan has livestreamed Masses, while pastors have multiplied their efforts to be close to the faithful via social media and phone.

The archbishop noted that, although Taiwan has diplomatic ties with very few countries, “the Catholic Church is present in every nation,” and therefore “the Church in Taiwan never feels isolated.” 

“On the contrary,” he said, “thanks to the apostolic nunciature and its representative, we truly feel the communion with the Holy Father and with other local churches.”

Meanwhile, the Church in Taiwan is planning for the future. 

Archbishop Hung said: “Our bishops’ conference has been preparing a national evangelization congress since last year, which was initially scheduled for August this year.”

“The congress will gather clergy and laity that will come together to pray, to reflect and to discuss different issues regarding the future of the Catholic Church in Taiwan. Due to the COVID-19 outbreak, the congress is now postponed to next year.”

He also noted that Taiwan could be a bridge-builder to the Chinese world.

“As a Chinese-speaking bishops’ conference in the Catholic Church, the Church in Taiwan can contribute to the evangelization of the Chinese-speaking people,” he said.

Taiwan’s bishops’ conference collaborates with the dioceses of Hong Kong and Macau to translate the pope’s messages and writings, as well as Vatican documents, into Chinese. 

“Evangelization presupposes inculturation and goes hand in hand with the teaching of the Catholic Faith,” the archbishop said, adding that his bishops’ conference was helping to make “the Catholic Faith relevant to the Chinese-speaking people all over the world.”

As per the normal review of market volatility to ensure adequate collateral coverage, the Chicago Mercantile Exchange Inc., Clearing House Risk Management staff approved the performance bond requirements for the following products listed in the advisory at the link below.

The rates will be effective after the close of business on April 22, 2020.

For the full text of this advisory, please click here.

As per the normal review of market volatility to ensure adequate collateral coverage, the Chicago Mercantile Exchange Inc., Clearing House Risk Management staff approved the performance bond requirements for the following products listed in the advisory at the link below.

The rates will be effective after the close of business on 4/23/2020.

For the full text of this advisory, please click here.

As per the normal review of market volatility to ensure adequate collateral coverage, the Chicago Mercantile Exchange Inc., Clearing House Risk Management staff approved the performance bond requirements for the following products listed in the advisory at the link below.

The rates will be effective after the close of business on 4/28/2020.

Click here for the full text of the advisory

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CME will be updating liquidity parameters for Interest Rate Swap contracts denominated in BRL and MXN. These changes only impact portfolios with larger BRL or MXN exposures. For MXN, the impact to liquidity add-on is approx. 5-10% for portfolios with aggregated DV01 above 100M+ DV01 in local currency. For BRL, the impact to liquidity add-on is 30%+ for portfolios with aggregated DV01 above 10M+ in local currency. Based on current exposures, CME Clearing expects that there will be no impact to existing portfolios at this time. These changes will be available for testing in our New Release environment April 29, 2020, with a production date of May 4, 2020.

Click here for the full text of the advisory

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As per the normal review of market volatility to ensure adequate collateral coverage, the Chicago Mercantile Exchange Inc., Clearing House Risk Management staff approved the performance bond requirements for the following products listed in the advisory at the link below.

The rates will be effective after the close of business on 05/01/2020.

Click here for the full text of the advisory

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The May 2020 Stock, ETF, and Corporate Bond update can be found at www.cmegroup.com/clearing/financial-and-collateral-management.html

Click here for the full text of the advisory

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Before I head to a new assignment, here are some final words on a few topics.

Affected by the dark reality of hopelessness she’s encountered in Madagascar, Caitlin Red prays that God will do miraculous things amongst the Malagasy people.

Starting a Christian micro-finance programme has been a long-time dream for OM Madagascar. In partnership with OM Hong Kong, this will soon become a reality.

Pastor Rene Armand, a pastor from the Androy region of Madagascar, shares how he became a pastor.

OM Madagascar responded to the declining literacy rate in the Androy region by starting a literacy course in Ambovombe. Last month the first class graduated, and the second class began.

An OMers love for people from the Island of Comoros led her to start a Discovery Bible Study for them at a university.

German translation of the BIS Press Release on the presentation of the Annual Report (25 June 2017)

Hungarian children get excited about the Easter message

The impact of baseball as an outreach tool is growing far beyond the first teams that began nine years ago in Érd.

An OMer in Hungary shares how God intervened in the lives of women in the sex industry through the Bus4Life ministry

OMer Gyöngyi served around the world, then the Riverboat led her back home to Hungary.

The African Union Development Agency says countries should think about how they choose the lesser of the two evils because strict lockdowns have severe consequences.

The Bank of England says the UK faces its worst slump in 300 years, but on Thursday held off from any moves on rates or bond buying.

The United Nations on Thursday issued a new appeal for $4.7 billion in funding to "protect millions of lives and stem the spread of coronavirus in fragile countries."

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Slow and inadequate progress in implementing the compromise they reached three years ago threatens to push Zimbabwe’s contending forces into premature elections and undermine political and economic recovery.

A bold approach to the sanctions issue is necessary to refocus efforts on the actions needed to break the political stalemate in Zimbabwe before elections are held that otherwise threaten to be as violent and undemocratic as the 2008 round.

To preserve Southern Africa’s relative peace in the face of rising challenges and threats, Southern African Development Community (SADC) member states must collectively reinforce its peace and security architecture.