In a new twist on oral history, University of Alberta paleontologists have discovered that an Argentinian reptile from 95 million years ago developed a type of tooth enamel that is common in humans and other mammals but rare among reptiles.

The Porcupine Health Unit is reporting the fifth COVID-19 related death in its area.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

Just across town from the Library of Congress in Washington, D.C., stands Gallaudet University, an institute for higher learning for the deaf and hard-of-hearing. In 1864, President Abraham Lincoln signed the law that allowed the school to begin issuing college degrees, a milestone for deaf people seeking higher education. Edward M. Gallaudet (right) was the […]

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

Colloidal gold–antibody conjugates are easy to prepare and are an excellent choice for microscopic applications. Colloidal gold is an aqueous suspension of nanometer-sized particles of gold. Typically, chloroauric acid, HAuCl₄, is reduced with dilute solutions of sodium citrate, as described here. This will cause the gold to form small aggregates that will associate with proteins. Gold particles of specific sizes can be isolated and differentiated microscopically, allowing these particles to be used for multiple-label experiments. Colloidal gold-labeled antibodies are widely used in electron microscopy (EM), and can be used for light microscopy but require additional steps (silver enhancement).

There are many uses for antibodies labeled with metal ions. Most of these methods involve first attaching a metal chelator to the antibody molecule. This is achieved using standard cross-linking chemistry and then adding the desired metal at appropriate concentration and pH. The method described here outlines a basic procedure for creating a lanthanide conjugate. Lanthanide conjugates are used for proximity assays, as MRI contrast agents, or for mass cytometry experiments. Different metals and chelators can be substituted, but the basic procedures are similar.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Our ongoing celebration of the Library of Congress National Book Festival continues with crime writer Patricia Cornwell discussing "Red Mist,” her 19th Kay Scarpetta novel, on the Fiction & Mystery stage at the 2012 Festival.

Our ongoing celebration of the Library of Congress National Book Festival continues with historian Michael Beschloss discussing “Presidents of War” on the Main Stage at the 2019 Festival.

Rural educator Frank Cyr had the vision and pull to force the nation to standardize the color of the ubiquitous vehicle

Press release about the Basel Committee publishing consultation paper on revisions to the credit valuation adjustment risk framework, 28 November 2019.

Press release: CPMI report sets out considerations for developers of wholesale digital tokens, 12 December 2019

Press release: Basel Committee invites comments on the design of a prudential treatment for crypto-assets, 12 December 2019.

Press release about the Basel Committee launching the consolidated Basel Framework, 16 December 2019.

Basel Committee Press release "Basel Committee meets to review vulnerabilities and emerging risks, advance supervisory initiatives and promote Basel III implementationl", 27 February 2020.

BIS statement, 16 March 2020.

Press release about Basel Committee assesses members' implementation of the Net Stable Funding Ratio and large exposures framework, 19 March 2020

Press release "Basel Committee coordinates policy and supervisory response to Covid-19", 20 March 2020

Press release "Governors and Heads of Supervision announce deferral of Basel III implementation to increase operational capacity of banks and supervisors to respond to Covid-19", 27 March 2020

BCBS Press release "Basel Committee sets out additional measures to alleviate the impact of Covid-19", 3 April 2020

BCBS Press release "Basel Committee and IOSCO announce deferral of final implementation phases of the margin requirements for non-centrally cleared derivatives", 3 April 2020

BCBS Press release "Basel III monitoring results based on end-June 2019 data published by the Basel Committee", 8 April 2020

BCBS Press release "Basel Committee issues progress report on banks' implementation of the 'Principles for effective risk data aggregation and reporting'", 29 April 2020

A protein shredder that occurs in cell membranes of brain cells apparently also indirectly regulates the fat metabolism.

Acute myeloid leukemia is an aggressive cancer of the blood-forming system. It affects the hematopoietic stem cells, or blood stem cells, of various white blood cells and of the red blood cells and platelets.

With a discovery that could rewrite the immunology textbooks, an international group of scientists, including the teams of Bart Lambrecht, Martin Guilliams, Hamida Hammad, and Charlotte Scott (all from the VIB-UGent Center for Inflammation Research) identified a new type of antigen-presenting immune cell.

Graduation is on hold for Dalhousie University medical student Manveer Bal, so he's decided to spend his newfound free time volunteering at the epicentre of the COVID-19 outbreak in Nova Scotia.

A Dalhousie University scientist and a NSCAD textile professor are teaming up to create a new fabric. It could be used to locally manufacture personal protective equipment like surgical masks. As the CBC's Colleen Jones reports, they have received a COVID-19 grant to study the idea.

People in Truro, N.S., are reeling after the disappearance of three-year-old Dylan Ehler as the community tries to come to grips with another potential tragedy in the area.

Police say efforts to find the driver of an abandoned car in Kings County last week likely saved his life.

Over the 13 hours the mass shooting took place across Nova Scotia on April 18 and 19, the RCMP called in resources from New Brunswick to help. However, the two municipal police forces closest to Portapique were not asked to respond directly at any point, CBC News has learned.

Asylum seekers make up a large portion of the "guardian angels" Quebec Premier François Legault has praised in his daily COVID-19 briefings: the orderlies who have no guarantee they'll be allowed to stay in Canada.

The provincially appointed trustee of the English Montreal School Board says the Quebec government made the “right decision” when it postponed the reopening of Montreal-area elementary schools to May 25.

Kristy-Lyn Kemp can't believe how close she came to potentially starting a COVID-19 outbreak at her new job.

Jean-Talon market has changed during the COVID-19 pandemic. Today, there are controlled entrances, someone making sure you douse your hands with sanitizer and another with a clicker in hand, counting the number of people who enter.

Here is what you should know as Quebec loosens travel restrictions in some regions.

Overcoming scorching heat and little rain, experimental vineyards teach winemakers to cope with climate change

Live professional baseball games could be televised in the United States as early next week, with South Korea's Yonhap News Agency reporting Monday that ESPN and the Korea Baseball Organization are nearing an agreement.

Vowing to return next summer, this year's Little League World Series was cancelled due to the coronavirus pandemic. All seven World Series tournaments and their respective regional qualifying events are off this summer.

Cleveland Indians reliever Emmanuel Clase was suspended 80 games by Major League Baseball on Friday for testing positive for a banned performance-enhancing drug.

Tampa Bay Rays' Blake Snell claimed champion status after defeating Lucas Giolito of the Chicago White Sox in the inaugural MLB The Show player league on Sunday.

"Recovery is not for the faint-hearted," says recovering addict Jeremy Raven. And sometimes, something as simple as a kind word may be the hand up that someone who is struggling needs, he says.

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 

They're not necessarily treating sick patients in hospitals, but a number of Manitoba-based scientists are working long hours and facing incredible pressure to battle the novel coronavirus from their labs and research facilities.

Shelley Luther (date: 5/9/2020 - Rank: 7)