Björn Eismann, Teresa G. Krieger, Jürgen Beneke, Ruben Bulkescher, Lukas Adam, Holger Erfle, Carl Herrmann, Roland Eils, and Christian Conrad

3D cell cultures enable the in vitro study of dynamic biological processes such as the cell cycle, but their use in high-throughput screens remains impractical with conventional fluorescent microscopy. Here, we present a screening workflow for the automated evaluation of mitotic phenotypes in 3D cell cultures by light-sheet microscopy. After sample preparation by a liquid handling robot, cell spheroids are imaged for 24 hours in toto with a dual-view inverted selective plane illumination microscope (diSPIM) with a much improved signal-to-noise ratio, higher imaging speed, isotropic resolution and reduced light exposure compared to a spinning disc confocal microscope. A dedicated high-content image processing pipeline implements convolutional neural network based phenotype classification. We illustrate the potential of our approach by siRNA knock-down and epigenetic modification of 28 mitotic target genes for assessing their phenotypic role in mitosis. By rendering light-sheet microscopy operational for high-throughput screening applications, this workflow enables target gene characterization or drug candidate evaluation in tissue-like 3D cell culture models.

The Hope Diamond’s red glow has long been considered a unique property of that stone. Most blue diamonds produce a bluish-white phosphorescence if exposed to ultraviolet light. The few other diamonds known to emit red phosphorescence were commonly assumed to have been from the even larger original stone from which the Hope was cut.

The post Bombarded with ultraviolet light, the blue Hope diamond glows red appeared first on Smithsonian Insider.

The prehistoric Xenicibis used its wings like two clubs hinged at the wrist joint in order to swing at and attack one another.

The post Prehistoric bird able to yield extreme fighting force with club-like wings appeared first on Smithsonian Insider.

A short history of the Harvard-Smithsonian Center for Astrophysics

The post Lighthouse of the skies, the Harvard-Smithsonian Center for Astrophysics appeared first on Smithsonian Insider.

One of Italy’s greatest treasures, Leonardo da Vinci’s Codex on the Flight of Birds, will be exhibited at the National Air and Space Museum from […]

The post da Vinci’s “Codex on the Flight of Birds” will be on view at Air and Space Museum, Sept. 13-Oct. 22. appeared first on Smithsonian Insider.

Click photo to take a tour of the 1903 Wright Flyer with Smithsonian X 3D Explorer. The Smithsonian X 3D Collection features objects from the […]

The post Take a 3D tour of the 1903 Wright Flyer! appeared first on Smithsonian Insider.

The National Air and Space Museum’s 2014 Trophy Award Winner for Current Achievement goes to the Dawn Flight Team. The Dawn team has successfully performed […]

The post The Dawn Flight Team – 2014 NASM Trophy Winner appeared first on Smithsonian Insider.

Each year, a mysterious group of night herons flock to Smithsonian’s National Zoo. Then, they vanish. In episode three of our series, we go behind […]

The post The great night heron mystery at the National Zoo appeared first on Smithsonian Insider.

This video is featured in the exhibition Beauty—Cooper Hewitt Design Triennial, on view at the museum February – August 2016. About: Aaron Koblin collaborates with […]

The post Aaron Koblin and Ben Tricklebank-Light Echoes appeared first on Smithsonian Insider.

The National Air and Space Museum will reopen the “Boeing Milestones of Flight Hall” July 1 in conjunction with the museum’s 40th anniversary. The two-year […]

The post Air and Space Museum’s “Boeing Milestones of Flight Hall” Reopens July 1! appeared first on Smithsonian Insider.

Timelapse video of Flight operations aboard the USS Dwight D. Eisenhower brought to you by the Smithsonian’s National Air and Space Museum.

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The contamination of Foster Farms chickens has provided insight into food regulation.

Steve Julian: Business analyst Mark Lacter, had we been paying attention before this happened?

Mark Lacter: You know, Steve, we often have an out of sight, out of mind attitude when it comes to food safety, and - as we're seeing with this episode - the government has a way of enabling that attitude.  What stands out, first of all, is that people started getting sick from salmonella-contaminated chicken back in March, and yet, it wasn't until the past few weeks that news stories began appearing about the seriousness of the problems.

Julian: At last check, more than 400 people have been infected, with most of them in California...

Lacter: Right, and Foster Farms, which is based in Merced County, controls two-thirds of the poultry market along the West Coast.  No fatalities so far, but many of the people who became sick had to be hospitalized - and that leads to still more concerns that the salmonella strains were resistant to antibiotics.  Now, why it took this long for consumers to be made aware that there was a problem tells you something about the way the federal government regulates poultry plants.  It was only last Friday, after the company had seen a 25 percent drop in sales, when the president of Foster Farms decided to go public.  He said he was embarrassed by the outbreak, and promised to change the company's processing facilities so that salmonella can be better identified.

Julian: Where was the US government in this?

Lacter: Apparently, the Department of Agriculture only requires testing for levels of salmonella at the time of slaughter - not later on, after the poultry is cut into parts.  Foster Farms now says it will do retesting at that later stage.  What's also interesting is that Foster Farms was not asked to recall any of its products because the chicken is considered safe as long as it's handled properly and then cooked to the right temperature, which is at least 165 degrees.  That's why some supermarkets have kept carrying the brand.

Julian: Can the government even order a recall?

Lacter: Not in a case like this - and that's because of a court case in the 1990s involving a Texas meat producer that federal inspectors were ready to shut down due to a salmonella outbreak involving ground beef.  The company sued the government, arguing that salmonella is naturally occurring, and therefore, not an adulterant subject to government regulation.  And the courts agreed.  Foster Farms has been using much the same argument.

Julian: Why isn't there more public outrage over this?

Lacter: Well, again, we go back to out of sight, out of mind.  Slaughterhouses are not exactly fun places, and they're usually not well covered by the news media until something bad happens, like the Foster Farms situation.

Julian: Chino comes to mind - a story we covered.

Lacter: That's when an animal rights group used a hidden camera to record inhumane treatment of cattle at a meat processing plant.  That company was forced into bankruptcy.  Another reason coverage is spotty is because it's not always easy to trace someone's illness to a contaminated piece of meat or chicken.  And, that leads to lots of misinformation.  The broader issue is figuring out a way to monitor these facilities without the process becoming cost prohibitive.  The Agriculture Department has been pushing a pilot program that would allow plants to speed up processing lines, and replace government inspectors with employees from the poultry companies themselves.

Julian: The idea being?

Lacter: The idea being to establish safeguards that can prevent problems before they get out of hand.  But, this is pretty controversial stuff, and advocacy groups representing poultry workers say that processing lines need to be slowed down, not speeded up.  So, you have this ongoing back and forth involving industry, government, consumer groups, and labor organizations.  And unfortunately, most of us tend to move on after one of these outbreaks gets cleared up.

Mark Lacter writes for Los Angeles Magazine and pens the business blog at LA Observed.com.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Obsessive in his collecting and emotionally invested in contributing to science and perpetuating knowledge, Palmer lived the adventurous yet nomadic life of a collector.

The post Remarkable ethnobotany collections of Edward Palmer highlighted in new Smithsonian Website appeared first on Smithsonian Insider.

Smooth and shiny, the tough body of the South African beetle Pedinorrhina trivittata, a flower eater, appears to be a non-inviting surface for pollen grains […]

The post Caught on camera: Despite hard shells pollen sticks to South African beetles appeared first on Smithsonian Insider.

Shallow-water seagrasses can’t survive without enough light. And fish, shrimp, crabs and other creatures we rely on for food can’t survive without seagrasses. Smithsonian biologist […]

The post Ecosystems on the Edge: Underwater Light and Seagrass appeared first on Smithsonian Insider.

By simulating the environment when corn was first exploited by people and then domesticated, Smithsonian scientists discovered that corn’s ancestor; a wild grass called teosinte, […]

The post Greenhouse “time machine” sheds light on corn domestication appeared first on Smithsonian Insider.

In a study published Sept. 28 in the journal Nature Plants, a team led by Smithsonian Conservation Biology Institute scientists found that bigger trees suffer […]

The post Forest Giants Suffer Most During Droughts appeared first on Smithsonian Insider.

Skinny lines of ants snake through the rainforest carrying leaves and flowers above their heads—fertilizer for industrial-scale, underground fungus farms. Soon after the dinosaur extinctions […]

The post Some ants still trying to get crop domestication right appeared first on Smithsonian Insider.

The Smithsonian is celebrating Earth Day this month by hosting the first Earth Optimism Summit from April 21 to 23 in Washington, D.C. Its goal […]

The post The Smithsonian’s history is right in line with Earth Optimism appeared first on Smithsonian Insider.

Visitors to the tropics are amazed by the huge variety of colorful, complex and sometimes ferocious creatures living near the equator. Smithsonian scientists and colleagues […]

The post Is This the Long-Sought Answer to the Question of Tropical Biodiversity? appeared first on Smithsonian Insider.

Hidden in plain sight–that’s how researchers describe their discovery of a new genus of large forest tree commonly found, yet previously scientifically unknown, in the […]

The post Remarkable new tree species was “hidden in plain sight” in the Andes appeared first on Smithsonian Insider.

According to a new study by biologists at the Smithsonian Migratory Bird Center and Virginia Tech the offspring of a certain songbird, the wood thrush, […]

The post Study: Large shady forest plots essential to survival of post-fledgling songbirds during drought appeared first on Smithsonian Insider.

Camouflage is a valuable survival strategy—just ask a chameleon. Scientists have just discovered a new form of mimicry camouflage: beetles that hide by chewing beetle-shaped […]

The post For millions of years these tiny beetles have chewed their way out of sight appeared first on Smithsonian Insider.

When creating a System Composer architecture model, if the following functions are called with an invalid or missing stereotype, it can cause the model to get corrupted and might lead to a MATLAB crash:

• addComponent
• addPort
• connect

Simulink might produce an incorrect sorted order for a model that meets all of the following conditions:

• The model contains blocks from the SoC Blockset
• The Signal logging option is selected in the model configuration set
• Signals using asynchronous sample time are configured for logging

The Open Banking Report 2019 clarifies the role of key key-players in a post-September 14th world and assesses how the landscape has shifted within Europe and beyond.

(The Paypers) CredoLab has partnered with iovation to integrate

(The Paypers) AML data and tech company Vital4 has revealed its partnership with UK-based

(The Paypers)

Yaya Wang
Apr 7, 2020; 133:jcs228072-jcs228072
REVIEW

Björn Eismann
Apr 15, 2020; 0:jcs.245043v1-jcs.245043
TOOLS AND RESOURCES

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Earth's magnetic field seems steady and true -- reliable enough to navigate by. Yet, largely hidden from daily life, the field drifts, waxes and wanes. The magnetic North Pole is currently shifting toward Siberia, forcing the Global Positioning System that underlies modern navigation to update its software sooner than expected. Every several hundred thousand years, the magnetic field dramatically shifts and reverses its polarity. Magnetic north flips to the geographic South Pole and, eventually, back again. This reversal has happened countless times over Earth's history, but scientists' understanding of why and how the field reverses is limited. The researchers find that the most recent field reversal 770,000 years ago took at least 22,000 years to complete, several times longer than previously thought. The results call into question controversial findings that some reversals could occur within a human lifetime.

Image credit: Brad Singer

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The Maya of Central America are thought to have been a kinder, gentler civilization, especially compared to the Aztecs of Mexico. At the peak of Mayan culture some 1,500 years ago, warfare seemed ritualistic, designed to extort ransom for captive royalty or to subjugate rival dynasties, with limited impact on the surrounding population. Only later, archeologists thought, did increasing drought and climate change lead to total warfare -- cities and dynasties were wiped off the map in so-called termination events -- and the collapse of the lowland Maya civilization around 1,000 A.D. (or C.E., current era). New evidence unearthed by National Science Foundation-funded researchers call all this into question, suggesting that the Maya engaged in scorched-earth military campaigns -- a strategy that aims to destroy anything of use, including cropland -- even at the height of their civilization, a time of prosperity and artistic sophistication. The finding also indicates that this increase in warfare, possibly associated with climate change and resource scarcity, was not the cause of the disintegration of the lowland Maya civilization.

Image credit: Francisco Estrada-Belli/Tulane

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National Science Foundation (NSF)-funded researchers from North Carolina State and Elon universities have developed a technique that allows them to remotely control the movement of soft robots, lock them into position for as long as needed and later reconfigure the robots into new shapes. The technique relies on light and magnetic fields. "By engineering the properties of the material, we can control the soft robot's movement remotely; we can get it to hold a given shape; we can then return the robot to its original shape or further modify its movement; and we can do this repeatedly. All of those things are valuable, in terms of this technology's utility in biomedical or aerospace applications," says Joe Tracy, a professor of materials science and engineering at NC State and corresponding author of a paper on the work. In experimental testing, the researchers demonstrated that the soft robots could be used to form "grabbers" for lifting and transporting objects. The soft robots could also be used as cantilevers or folded into "flowers" with petals that bend in different directions. "We are not limited to binary configurations, such as a grabber being either open or closed," says Jessica Liu, first author of the paper and a Ph.D. student at NC State. "We can control the light to ensure that a robot will hold its shape at any point."

Image credit: Jessica A.C. Liu

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How do galaxies such as our Milky Way come into existence? How do they grow and change over time? The science behind galaxy formation has long been a puzzle, but a University of Arizona-led team of scientists is one step closer to finding answers, thanks to supercomputer simulations. Observing real galaxies in space can only provide snapshots in time, so researchers who study how galaxies evolve over billions of years need to use computer simulations. Traditionally, astronomers have used simulations to invent theories of galaxy formation and test them, but they have had to proceed one galaxy at a time. Peter Behroozi of the university's Steward Observatory and colleagues overcame this hurdle by generating millions of different universes on a supercomputer, each according to different physical theories for how galaxies form. The findings challenge fundamental ideas about the role dark matter plays in galaxy formation, the evolution of galaxies over time and the birth of stars. The study is the first to create self-consistent universes that are exact replicas of the real ones -- computer simulations that each represent a sizeable chunk of the actual cosmos, containing 12 million galaxies and spanning the time from 400 million years after the Big Bang to the present day. The results from the "UniverseMachine," as the authors call their approach, have helped resolve the long-standing paradox of why galaxies cease to form new stars even when they retain plenty of hydrogen gas, the raw material from which stars are forged. The research is partially funded by NSF's Division of Physics through grants to UC Santa Barbara's Kavli Institute for Theoretical Physics and the Aspen Center for Physics.

Image credit: NASA/ESA/J. Lotz and the HFF Team/STScI

A bold national initiative is needed to reduce the enormous health burden of sexually transmitted diseases (STDs) in the United States, according to a new report from a committee of the Institute of Medicine.

More targeted aircraft testing and simulation should be conducted to uncover design characteristics in new flight control systems that -- in rare circumstances -- may mislead pilots and result in unstable or dangerous flight conditions, says a new report by a National Research Council committee.