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One of the main mechanisms of azole resistance of Aspergillus fumigatus is thought to be a reduction in the drug’s affinity for the target molecule, Cyp51A, due to its amino acid mutation(s). It is known that the azole resistance pattern is closely related to the mutation site(s) of the molecule. In this study, we tried to develop a simple and rapid detection method for cyp51A mutations using the endonuclease Surveyor nuclease. The Surveyor nuclease assay was verified using several azole-resistant strains of A. fumigatus that possess point mutations in Cyp51A. For validation of the Surveyor nuclease assay, blind tests were conducted using 48 strains of A. fumigatus (17 azole-resistant and 31 azole-susceptible strains). The Surveyor nuclease assay could rapidly detect cyp51A mutations with one primer set. Also, all the tested strains harboring different cyp51A single point mutations could be clearly distinguished from the wild type. The Surveyor nuclease assay is a simple method that can detect cyp51A mutations rapidly.

FSI Briefs No 5, April 2020. In response to the Covid-19 pandemic, governments have launched guarantee programmes to support bank lending to companies, especially small and medium-sized enterprises. This is essential to avoid a sharp contraction in bank credit that would exacerbate the pandemic's adverse impact. The design of such programmes needs to strike a difficult balance between responding promptly to the pandemic and maintaining a sufficient level of prudence. Key features of a sample of programmes (eg target beneficiaries, coverage of the guarantee, loan terms, length of the programme) reflect this tension. Incentives were created for the banks to join these programmes by exploiting flexibility in existing prudential requirements, while central banks have often provided liquidity support. Programmes are, however, subject to operational challenges and, ultimately, fiscal capacity limits.

Close to 300 series at various frequencies for 60 countries have been updated.

Monthly and annual data have been updated for 60 countries. The average length of the monthly series is more than to 55 years. Some annual series go back to the middle of the 19th century - or even earlier for several countries.

Advanced preliminary release for Q4 2019 quarterly data on debt service ratios (DSRs) for the private non-financial sector (PNFS) for 32 countries have been updated. DSRs for PNFS breakdown sectors, ie for households and for non-financial corporations are also available for 17 countries.

The BIS's data set on consumer prices contains long monthly and annual time series for 60 countries.

Remarks by Mr Stephen S Poloz, Governor of the Bank of Canada, at the Ivey Business School, London, Ontario, 30 April 2020.

Introductory remarks (by videoconference) by Mr Luis de Guindos, Vice-President of the European Central Bank, to the Committee on Economic and Monetary Affairs of the European Parliament, Frankfurt am Main, 7 May 2020.

Notional amounts of OTC derivatives rose from $544 trillion at end-December 2018 to $559 trillion at end-December 2019. This, however, corresponds to a significant fall over the last six months, which could be at least partly attributed to seasonal factors. The seasonal pattern in the data, whereby end-June values tend typically to be larger than end-of-year values, appears strongest in positions vis-à-vis central counterparties. Such end-of-year contractions can occur if dealer banks and/or their clients shrink their outstanding notional derivative positions for regulatory and financial reporting purposes. Contracts denominated in non-G4 currencies (ie excluding USD, EUR, JPY and GBP) have grown in size for both interest rate and foreign exchange derivatives, even though those denominated in US dollars remain the largest segment.

Daily data on effective exchange rate in nominal terms have been updated. Broad indices cover 60 economies, with data from 1996. Narrow indices cover 26 economies with data from 1983.

Daily data on monetary policy rates have been updated. This covers 38 central banks with the earliest data starting in 1946.

Long-term interest rates have remained low despite a surge in the issuance of sovereign debt to mitigate the economic impact of the pandemic.

Inflation pressure could be weak even after consumer demand for non-discretionary goods and services begins to grow as the economy gets back on its feet.

At a time of conflicting narratives, a range of markets from equities to energy to gold may persist in volatility until one narrative gains the upper hand.

Gold and silver prices often move in tandem, but the gap between them widened by 31% January through April in line with a trend that began nine years ago.

A look at support and regenerative policy initiatives by the Federal Reserve and the Government as more than 20 million jobs were lost in April 2020.

Bank of Italy Working Papers by Luca Metelli, Filippo Natoli and Luca Rossi

Bank of Italy Working Papers by Andrea Gazzani and Alejandro Vicondoa

Bank for International Settlements BIS Working Papers by David Cook and Nikhil Patel

Bank of England Working Papers by Aydan Dogan and Ida Hjortsoe

Bank of England Working Papers by Cristiano Cantore and Lukas Freund

Bank of England Working Papers by Cristiano Cantore, Filippo Ferroni and Miguel León-Ledesma

Netherlands Bank DNB Occasional Studies by Peter Wierts and Harro Boven

European Central Bank Working Papers by Marien Ferdinandusse, Maximilian Freier and Annukka Ristiniemi

European Systemic Risk Board Occasional Papers by Greg Ros

Bank of Spain Working Papers by Henrique S. Basso and Juan F. Jimeno

Central Bank of Chile Working Papers by Andrés Sagner

Central Bank of Chile Working Papers by Benjamín García and Juan Guerra-Salas

Central Bank of Chile Working Papers by Tomás Gómez, Alejandro Jara and David Moreno

Central Bank of Chile Working Papers by Gee Hee Hong, Matthew Klepacz, Ernesto Pasten and Raphael Schoenle

More suffering is ahead for the developing world.

These causes will make great use of your money or your time.

Ready to "switch" things up on your desktop? A mechanical keyboard can make everything click again. Here's a guide to the most common mechanical key types, as well as the top performers in our testing.

Cord cutters have more choices than just Netflix and Hulu. We break down the best video streaming services for your money, whether you're looking to completely replace cable or watch the latest original content on-demand.

Filmic Pro is a mobile app designed to turn your smartphone into a professional video camera. It comes impressively close.

Frankly, Trump doesn’t give a damn.

In this nightmarish moment, we’re feeling warm and fuzzy about the cold and calculating Andrew Cuomo.

As college campuses closed and the COVID-19 virus began casting a larger shadow in south central Pennsylvania, members of the nursing and physical therapist assistant programs at Penn State Mont Alto took stock of all the medical personal protection equipment (PPE) items in their labs. The two programs were supplied with hundreds of gloves and dozens of medical gowns and masks, plus other items such as disposable stethoscopes, shoe covers and safety glasses — items no longer needed as the campus transitioned to remote instruction.

Penn State Mont Alto recognizes and honors winners during awards ceremony

Authoritarian rule may be just around the corner.

Billions for oil, nothing for nurses and teachers.

The only fiscal thing to fear is deficit fear itself.

Why won’t Republicans help Americans losing their jobs?

The College of Health and Human Development will host M. Daniele Fallin, Sylvia and Harold Halpert Professor in Mental Health and chair of the Department of Mental Health at the Johns Hopkins Bloomberg School of Public Health, at 4 p.m. via Zoom Webinar on Thursday, May 7, for the next presentation in its Dean’s Lecture Series: Perspectives on the Pandemic. This presentation, “Mental Health and the COVID Pandemic,” will summarize recent findings on the psychological effects of the pandemic, as well as offer some strategies for prevention and intervention as the pandemic, and its after-effects, continue.

The 2020 Special Olympics Summer Games, slated for June 11 to 13, are being canceled due to concerns related to coronavirus. The games will shift to virtual events, with details to be announced in the near future.

Apple's latest iOS 13.5 beta release includes a new Health app feature that allows iPhone and Apple Watch users to automatically send Medical ID information to first responders.