When the heat and humidity of the Washington, D.C. summer sends its residents scrambling for air conditioning and iced coffee, the animal care specialists at […]

The post How do National Zoo animals beat the heat? Bloodsicles and other frozen delicacies appeared first on Smithsonian Insider.

Death-associated protein kinase 1 (DAPK1) is a serine/threonine protein kinase that regulates apoptosis and autophagy. DAPK1 is considered to be a therapeutic target for amyloid-β deposition, endometrial adenocarcinomas and acute ischemic stroke. Here, the potent inhibitory activity of the natural anthraquinone purpurin against DAPK1 phosphorylation is shown. Thermodynamic analysis revealed that while the binding affinity of purpurin is similar to that of CPR005231, which is a DAPK1 inhibitor with an imidazopyridazine moiety, the binding of purpurin was more enthalpically favorable. In addition, the inhibition potencies were correlated with the enthalpic changes but not with the binding affinities. Crystallographic analysis of the DAPK1–purpurin complex revealed that the formation of a hydrogen-bond network is likely to contribute to the favorable enthalpic changes and that stabilization of the glycine-rich loop may cause less favorable entropic changes. The present findings indicate that purpurin may be a good lead compound for the discovery of inhibitors of DAPK1, and the observation of enthalpic changes could provide important clues for drug development.

Between the world wars of the early Twentieth Century, an age of adventure travel and cultural exploration flourished when newly developed transport and recording technologies–particularly […]

The post New Book: “Recreating First Contact: Expeditions, Anthropology, and Popular Culture” appeared first on Smithsonian Insider.

Rock ’n’ roll musicians live forever in the mind’s eye thanks to iconic photos of them in their element, playing live: Chuck Berry and his […]

The post What makes a great rock ’n’ roll photo? appeared first on Smithsonian Insider.

Xian Hu, Salma Jalal, Michael Sheetz, Oddmund Bakke, and Felix Margadant

Despite progress made in confocal microscopy, even fast systems still have insufficient temporal resolution for detailed live cell volume imaging, such as tracking rapid movement of membrane vesicles in three-dimensional space. Depending on the shortfall, this may result in undersampling and/or motion artifacts that ultimately limit the quality of the imaging data. By sacrificing detailed information in the Z-direction, we propose a new imaging modality that involves capturing fast "projections" from the field of depth which shortens imaging time by approximately an order of magnitude as compared to standard volumetric confocal imaging. With faster imaging, radiation exposure to the sample is reduced, resulting in less fluorophore photobleaching and potential photodamage. The implementation minimally requires two synchronized control signals that drive a piezo stage and trigger the camera exposure. The device generating the signals has been tested on spinning disk confocals and instant structured-illumination-microscopy (iSIM) microscopes. Our calibration images show that the approach provides highly repeatable and stable imaging conditions that enable photometric measurements of the acquired data, in both standard live imaging and super-resolution modes.

Kasun Buddika, Ishara S. Ariyapala, Mary A. Hazuga, Derek Riffert, and Nicholas S. Sokol

Stressed cells downregulate translation initiation and assemble membrane-less foci termed stress granules (SGs). Extensively characterized in cultured cells, the existence of such structures in stressed adult stem cell pools remain poorly characterized. Here we report that Drosophila orthologs of mammalian SG components AGO1, ATX2, CAPRIN, eIF4E, FMRP, G3BP, LIN-28, PABP, and TIAR are enriched in adult intestinal progenitor cells where they accumulate in small cytoplasmic messenger ribonucleoprotein complexes (mRNPs). Treatment with sodium arsenite or rapamycin reorganized these mRNPs into large cytoplasmic granules. Formation of these intestinal progenitor stress granules (IPSGs) depended on polysome disassembly, led to translational downregulation, and was reversible. While canonical SG nucleators ATX2 and G3BP were sufficient for IPSG formation in the absence of stress, neither of them, nor TIAR, either individually or collectively, were required for stress-induced IPSG formation. This work therefore finds that IPSGs do not assemble via a canonical mechanism, raising the possibility that other stem cell populations employ a similar stress-response mechanism.

Julian Chollet, Alexander Dünkler, Anne Bäuerle, Laura Vivero-Pol, Medhanie A. Mulaw, Thomas Gronemeyer, and Nils Johnsson

Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of the septins to this prospective bud site is one of the critical events in a complex assembly pathway that culminates in the outgrowth of a new daughter cell. Hereby, the septin-rods follow the high concentration of Cdc42_GTP that is generated by the focused localization of its GEF Cdc24. We show that shortly before budding Cdc24 not only activates Cdc42 but also transiently interacts with Cdc11, the septin subunit that caps both ends of the septin rods. Mutations in Cdc24 reducing the affinity to Cdc11 impair septin recruitment and decrease the stability of the polarity patch. The interaction between septins and Cdc24 thus reinforces bud assembly at sites where septin structures are formed. Once the septins polymerize into the ring, Cdc24 is found at the cortex of the bud and directs its further outgrowth from this position.

Formed some 250,000 years ago when the Nile River pushed through a low channel near Wadi Tushka, it flooded the eastern Sahara, creating a lake that at its highest level covered more than 42,000 square miles.

The post Ancient megalake discovered beneath Sahara Desert appeared first on Smithsonian Insider.

John Ososky, preparator in the Osteology Laboratory at the Smithsonian's National Museum of Natural History in Washington, D.C., explains how skeletons of animal specimens are prepared for exhibtion and for study—with the assistance of nearly 1 million flesh-eating beetles.

The post Flesh Eating beetles help prepare skeletons for study at the Smithsonian’s Natural History Museum appeared first on Smithsonian Insider.

"The Antarctic Treaty Summit: Science-Policy Interactions in International Governance" will be convened at the Smithsonian Institution, National Museum of Natural History in Washington, DC from Nov. 30 to Dec. 3 2009. More information on this event can be found at the Web site: www.atsummit50.aq/

To assess legacy lessons about managing nearly 10% of the Earth "for peaceful purposes only"

Find out how you can be involved:

www.atsummit50.aq

The post Antarctic Treaty Summit, Nov. 30-Dec. 3, 2009, Smithsonian, Washington, D.C. appeared first on Smithsonian Insider.

What does it take to care for a pride of lions? Go behind the scenes with Great Cats Curator Craig Saffoe as he works with animal keepers and veterinarians to prepare the National Zoo's frisky lion cubs for their public debut. For more about the Zoo's growing pride and to watch them on live webcams: http://nationalzoo.si.edu/Animals/GreatCats/default.cfm?cam=LC4

The post Great Cats curator Craig Saffoe discusses his work caring for the National Zoo’s seven frisky lion cubs appeared first on Smithsonian Insider.

The post “How Soil Savvy are You?, is featured in the exhibition “Dig It! The Secrets of Soil,” from the Smithsonian Traveling Exhibition Service appeared first on Smithsonian Insider.

The post Creating a dictionary: Gabriela Pérez Báez, Curator of Linguistics appeared first on Smithsonian Insider.

The post Smithsonian conservators open 2001 Jim Thorpe Wheaties Box from the Smithsonian’s National Museum of the American Indian appeared first on Smithsonian Insider.

Meet Rachel Page, a Smithsonian scientist in Panama who studies frog-eating bats (fringe-lipped bats), among other topics. Her current research focuses on learning and memory in neotropical bats, combining field studies with laboratory experiments to learn about predator cognition and its effects on the evolution of their prey.

The post Meet our Scientist Rachel Page. She studies frog-eating bats, and other animals, in Panama appeared first on Smithsonian Insider.

The post Short video featuring the fieldwork of Smithsonian scientists created in 2000, posted by the Smithsonian Archives appeared first on Smithsonian Insider.

The post What will invasive round goby fish do to Great Lakes streams? Join SERC post doc Matt Kornis and find out. appeared first on Smithsonian Insider.

What is it like to be the curator of Great Cats at the National Zoo? We interviewed Craig Saffoe to hear firsthand.

The post What is it like to be curator of Great Cats at the National Zoo? appeared first on Smithsonian Insider.

Each year, a mysterious group of night herons flock to Smithsonian’s National Zoo. Then, they vanish. In episode three of our series, we go behind […]

The post The great night heron mystery at the National Zoo appeared first on Smithsonian Insider.

Ever wonder why only male peacocks have such extravagant plumage? We ask caretaker Gwendolyn Cooper at Smithsonian’s National Zoo to explain.

The post Why are male peacock feathers more colorful appeared first on Smithsonian Insider.

  Frances Glessner Lee created dollhouses with dead dolls. Her creations are now on exhibit at the Renwick Gallery of the Smithsonian American Art Museum.

The post The dollhouses of death that changed forensic science appeared first on Smithsonian Insider.

At the Smithsonian’s National Museum of Natural History, curator of arachnids and myriapods Hannah Wood has examined and analyzed hundreds of pelican spiders both in the field […]

The post Pelican spiders: Ancient assassins that eat their own kind appeared first on Smithsonian Insider.

Latinus 9333 is the Latin translation of the so-called Tacuinum sanitatis, a medieval handbook on wellness written in Arabic by the 11th-century physician ibn Butlan. It deals with factors influencing human health: from the air, the environment and food, to physical exercise and sexual activity.

The post Medieval book is important resource for how plants were once collected, treated and used appeared first on Smithsonian Insider.

Invasive European earthworms could prevent roughly half a North American forest’s orchid seeds from even germinating, ecologists from Smithsonian Environmental Research Center and Johns Hopkins […]

The post Invasive earthworms threaten wild American orchids appeared first on Smithsonian Insider.

South American rainforests thrived during three extreme global warming events in the past, say paleontologists at the Smithsonian Tropical Research Institute in a new report […]

The post Smithsonian scientists discover that rainforests take the heat appeared first on Smithsonian Insider.

Mexico is the fourth largest honey producer and fifth largest honey exporter in the world. A Smithsonian researcher and colleagues helped rural farmers in Mexico […]

The post Genetically modified soybean pollen threatens Mexican honey sales appeared first on Smithsonian Insider.

Carnivorous plants are a fascinating example of nature at its best. Living in habitats with nutrient-poor soil, carnivorous plants evolved to attract some insects as […]

The post How Carnivorous Plants avoid eating their pollinating insect friends appeared first on Smithsonian Insider.

How much is a wetland worth? It’s a question that has plagued policymakers, scientists and other leaders looking to protect their communities and slow down […]

The post New report enables creation of carbon credits for restored wetlands appeared first on Smithsonian Insider.

By now, most of the United States has started to feel the first cool caresses of winter. Everything is pumpkin spiced, and the last crickets […]

The post Feathered diplomats unite pupils in North and Central America appeared first on Smithsonian Insider.

Dead zones affect dozens of coral reefs around the world and threaten hundreds more according to a new study by Smithsonian scientists published in the […]

The post Dead Zones May Threaten Coral Reefs Worldwide appeared first on Smithsonian Insider.

A tiny invasive insect from Asia might have an effect on Chesapeake Bay waters. The emerald ash borer is killing millions of ash trees in […]

The post Invasive ash borer found in Smithsonian Environmental Research Center forest; ash deaths may impact Chesapeake waters appeared first on Smithsonian Insider.

File: (L-R) "The Sarah Silverman Program" writer Harris Wittels, comedian Sarah Silverman, executive producer/head writer Dan Sterling and actress Laura Silverman, arrive at Comedy Central's Emmy Awards party at the STK restaurant Sept. 21, 2008 in Los Angeles.; Credit: Ethan Miller/Getty Images

Harris Wittels, a comedy writer who worked on "Parks & Recreation," has died at 30, the Los Angeles Police Department's Jane Kim tells KPCC.

Wittels was discovered by his assistant around 12 p.m., Kim said, and was already dead. Kim said that Wittels' death was a possible overdose, but that the Coroner's Office would determine the cause of death. Wittels had attended drug rehab twice.

Comedy Central, where Wittels worked on "The Sarah Silverman Program" and "Secret Girlfriend," confirmed Wittels' death, as did the comedy show he appeared at Wednesday night.

Comedy Central tweet

Meltdown Show tweet

Wittels was also well known for his @Humblebrag Twitter account and later book, helping to popularize the idea online of the false modesty of bragging while trying not to look like you're bragging.

Wittels had spoken about his struggles with addiction in places including Pete Holmes's podcast "You Made It Weird" in a November episode.

"I just really stopped caring about my life," Wittels said on "You Made It Weird," explaining how he got into doing drugs. "I just really started to think, well, if I'm only here for 80 years, then who cares if I spend it high or not?"

Wittels received his first big break when Sarah Silverman saw him performing comedy and gave him a job writing for her Comedy Central show.

Wittels also wrote for HBO's "Eastbound & Down," several MTV awards shows and the American Music Awards. He had a recurring role on "Parks & Recreation" and was a regular guest on the "Comedy Bang Bang" podcast.

Comedians, actors and fans mourned Wittels' death online.

Harris Wittels Storify

See Wittels in a scene from "Parks & Recreation":

Wittels on Parks & Recreation

Listen to Wittels on "Comedy Bang Bang":

Wittels on Comedy Bang Bang

This story has been updated.

This content is from Southern California Public Radio. View the original story at SCPR.org.

A Nintendo Entertainment System.; Credit: Mark Ramsay/Flickr Creative Commons

There have been plenty of video game movies over the years, but there have been far fewer actually good ones. "Ready Player One," based on the 2011 video game-inspired novel, has the chance to be a great one thanks to the announcement that Steven Spielberg has signed on to direct.

That book was inspired by classic video games, and was written using classic video game cheats to play parts of classic games and write them into his book, author Ernest Cline said in a recent talk. That got us thinking about the classic video game cheats and secrets that stuck with us from our younger days playing classic video games — here's our top 11.

1. The Konami Code

Up up down down left right left right B A start! This code became such a part of video game culture that it got its own name. It was popularized in various games made by Konami, particularly Contra, leading to it also being known as the "Contra Code" for its ability to give you 30 lives in the game. Before the Internet, it was spread through gaming magazines and word of mouth — it was so influential that there are still developers who put it in their games. (There's even an entire Wikipedia page of games, both from Konami and others, that use the Konami Code. It's even been used on some websites.)

What is the Konami Code

2. Street Figher II Turbo's turbo

The game that I actually used a code for the most as a kid, the Super Nintendo code down, R, up, L, Y, B on the second controller didn't give you any advantages — it just kicked the speed up. By default, you had a few selections for how fast the game would be, but you could multiple that several-fold with this code, letting you and your friends battle at what at the time felt unbelievably fast.

3. Super Mario Bros.'s Minus World

This one doesn't involve a code, but players managed to discover what was deemed a glitch in the game that put you into a messed up version of another level, dubbed by fans the Minus World due to just "-1" instead of a full level number appearing at the top of the screen. There was no way to escape the glitched level, no matter how hard you might try, sending you to play it over and over again until your time ran out or you were killed by enemies. Still, modern players have found that you can go on thanks to various computer emulators and the like; see some of the worlds beyond below:

Minus World video

4. Metroid and Justin Bailey

Fans early on discovered that the password JUSTIN BAILEY allowed you to start with all of the available weapons along with plenty of life and ammo. Fans didn't know whether Justin Bailey was a reference to an actual person, just a code coincidence or something else, but that didn't stop them from eagerly playing through with this code. It also removed lead character Samus Aran from her armor, allowing players to discover that the game's star was an early female lead character, even if her armor didn't clue players in before the end of the game without the code.

Justin Bailey video

5. Doom's God mode

By typing the letters iddqd in PC game Doom, players could enable God mode, making them essentially invincible and letting them power through the early first-person shooter. The code had been available in developer id's earlier game Wolfenstein, but hadn't been quite as easily accessible. So, if you ever need a power boost when you're fighting on Mars, Doom has the answer. (Unfortunately, we don't believe this provides any extra protection for NASA's Mars rovers.)

6. Mortal Kombat's Reptile

The developers of Mortal Kombat made a battle against Reptile unbelievably hard for Mortal Kombat fans, including putting some randomness into whether doing what you were supposed to do to unlock the character would even work. Still, players happily pumped in extra quarters for the chance to face off against Reptile, a character with a look that mirrored that of characters Sub-Zero and Scorpion, just with a different color. Players had to achieve a Double Flawless victory on the Pit level, finish the match using their fatality move, and there also had to be a silhouette flying past the moon in the background — which only happened every sixth game.

Mortal Kombat: Reptile

7. The Legend of Zelda's Second Quest

The Legend of Zelda was a pretty challenging early adventure game, one of the first releases for the Nintendo home video game console. When you beat the game, you were given the option to go on a "Second Quest," which was a tweaked version of the game you just played except waaaaay harder. However, if you thought you were the coolest kid on the block and were so awesome you didn't need a warmup, you could name your character "ZELDA" (in one of the more obvious cheats in video game history) and skip ahead to that Second Quest from the start. You would then likely cry from how hard it was and start another new game with a different name.

8. NBA Jam's celebrity secret characters

A variety of celebrities from sports, music, and even politics were available by putting in various initials combined with buttons on the controller. These included then-President Bill Clinton, Al Gore, Warren Moon and more. That tradition has been continued in more recent NBA Jam games, with President Obama and others available for your video gaming pleasure.

9. GoldenEye's extra modes

There were a wide variety of cheat codes for GoldenEye, widely considered one of the all-time great first-person shooters. Sure, you could use codes to unlock different levels, but the reason these codes are remembered is because it gave you all sorts of new ways to play against your friends. The game also had an actual cheat menu that would appear if you accomplished one of a variety of goals, and from that menu you could cheat extra hard by using your controller to unlock things like a paintball mode, turbo mode, modes with both giant heads and teeny-tiny James Bonds and more.

10. Sonic The Hedgehog 2's Debug Mode

The Sonic debug mode is the perfect example of why so many games included cheats back in the day: They were often for the developers to be able to more easily play the games while looking for bugs and doing other testing. Sonic 2 let you get to an actual debug mode by playing various sounds from the level select screen (1, 9, 9, 2, 1, 1, 2, 4, for your reference), then pressing start and holding the A button. Developers later put them in for fun and intended them for the players to discover, but some of the early cheat codes were just meant for developers — but players proved more intrepid than they may have anticipated.

Sonic debug mode

11. Mike Tyson's Punch-Out: Go straight to Mike Tyson

There was actually a password mode in this game — before games had the option to save, plenty of games gave you codes that let you get back to where you were before. This is one of the examples from that darker time, where whether you played through opponents like Glass Joe and Bald Bull or not, you could try your changes against Lightning Mike (at least until the video game's license ran out and he was replaced in future editions with the way less exciting "Mr. Dream"). You better have had a pen and paper ready when your friend started yelling at you 007 373 5963 for you to use on your own copy of the game — no sharing. The game is hard enough that even Mike Tyson had some trouble fighting himself:

Mike Tyson vs. Mike Tyson

Let us know in the comments the classic video games — and the classic cheat codes and secrets — that inspired you.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Full Text:

Needle pricks not your thing? A team of National Science Foundation-funded scientists is developing wearable skin sensors that can detect what's in your sweat. They hope that one day, monitoring perspiration could bypass the need for more invasive procedures like blood draws, and provide real-time updates on health problems such as dehydration or fatigue. In a new paper, the team describes a new sensor design that can be rapidly manufactured using a "roll-to-roll" processing technique that essentially prints the sensors onto a sheet of plastic like words on a newspaper. They used the sensors to monitor the sweat rate, and the electrolytes and metabolites in sweat, from volunteers who were exercising, and others who were experiencing chemically induced perspiration. The new sensors contain a spiraling microscopic tube, or microfluidic, that wicks sweat from the skin. By tracking how fast the sweat moves through the microfluidic, the sensors can report how much a person is sweating, or their sweat rate. The microfluidics are also outfitted with chemical sensors that can detect concentrations of electrolytes like potassium and sodium, and metabolites like glucose.

Image credit: Bizen Maskey/Sunchon National University

Full Text:

What an animal eats is a fundamental aspect of its biology, but surprisingly, the evolution of diet had not been studied across the animal kingdom until now. Scientists at the University of Arizona report several unexpected findings from taking a deep dive into the evolutionary history of more than one million animal species and going back 800 million years, when the first animals appeared on our planet. The study revealed several surprising key insights: Many species living today that are carnivorous, meaning they eat other animals, can trace this diet back to a common ancestor more than 800 million years ago; A plant-based, or herbivorous, diet is not the evolutionary driver for new species that it was believed to be; Closely related animals tend to share the same dietary category -- plant-eating, meat-eating, or both. This finding implies that switching between dietary lifestyles is not something that happens easily and often over the course of evolution.

Image credit: Daniel Stolte/UANews

Source: Streetwise Reports   05/07/2020

In a May 5 research note, analyst John Freeman reported that Raymond James increased its target price on Parsley Energy, Inc. (PE:NYSE) after it posted its Q1/20 numbers.

Raymond James' new target price on Parsley is $12 per share, up from $11. The Texas-based energy company's stock is trading now at about $9.38 per share.

Freeman reviewed and commented on Parsley's Q1/20 results. The company "delivered modest EBITDA and earnings per share beats relative to the Street" due to oil pricing," Freeman pointed out.

Production was relatively in line at 126,600,000 barrels of oil per day (126.6 MMbbl/d), which was 1% higher than consensus' forecast but 1% below Raymond James' estimate. Total production was 1% above the Street's projection but 3% below Raymond James' forecast.

"The performance on the quarter was encouraging, however, the highlight from earnings was the significant reduction in 2020 capex (down from about $1 billion to less than $700 million)," Freeman commented.

Capex, "a welcome surprise," Freeman wrote, came in 5% and 7% lower than the investment bank and the Street's estimates, respectively. Opex was 3% under Raymond James' projection

Moreover, Parsley's related maintenance capital needs were greatly below expectations as well, indicating that Parsley made capital efficiency gains during the period.

"We were pleasantly surprised that Parsley is able to maintain in line Q4/20 oil volumes (about 115 MMbbl/d) on a capital program that's about $300 million/30% below the Street," added Freeman.

Looking forward, Raymond James modeled a base case, or stable scenario for Parsley, that implies a West Texas Intermediate oil price of about $30 a barrel and Parsley having four to five rigs and one to two crews operating. In that scenario, Parsley would produce about 117 MMbbl/d in 2020 and 115 MMbbl/d in 2021.

Capex would amount to about $678 million in 2020, dropping to $598 million in 2021.

Free cash flow would be about $300 million in 2020, which coincides with Parsley's guidance of $300M plus, and increasing to $370 million in 2021.

Raymond James has an Outperform rating on Parsley Energy.

Disclosure:
1) Doresa Banning compiled this article for Streetwise Reports LLC and provides services to Streetwise Reports as an independent contractor. She or members of her household own securities of the following companies mentioned in the article: None. She or members of her household are paid by the following companies mentioned in this article: None.
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3) Comments and opinions expressed are those of the specific experts and not of Streetwise Reports or its officers. The information provided above is for informational purposes only and is not a recommendation to buy or sell any security.
4) The article does not constitute investment advice. Each reader is encouraged to consult with his or her individual financial professional and any action a reader takes as a result of information presented here is his or her own responsibility. By opening this page, each reader accepts and agrees to Streetwise Reports' terms of use and full legal disclaimer. This article is not a solicitation for investment. Streetwise Reports does not render general or specific investment advice and the information on Streetwise Reports should not be considered a recommendation to buy or sell any security. Streetwise Reports does not endorse or recommend the business, products, services or securities of any company mentioned on Streetwise Reports.
5) From time to time, Streetwise Reports LLC and its directors, officers, employees or members of their families, as well as persons interviewed for articles and interviews on the site, may have a long or short position in securities mentioned. Directors, officers, employees or members of their immediate families are prohibited from making purchases and/or sales of those securities in the open market or otherwise from the time of the interview or the decision to write an article until three business days after the publication of the interview or article. The foregoing prohibition does not apply to articles that in substance only restate previously published company releases.

Disclosures from Raymond James, Parsley Energy Inc, May 5, 2020

ANALYST INFORMATION

Analysts Holdings and Compensation: Equity analysts and their staffs at Raymond James are compensated based on a salary and bonus system. Several factors enter into the bonus determination, including quality and performance of research product, the analyst's success in rating stocks versus an industry index, and support effectiveness to trading and the retail and institutional sales forces. Other factors may include but are not limited to: overall ratings from internal (other than investment banking) or external parties and the general productivity and revenue generated in covered stocks.

The analyst John Freeman, primarily responsible for the preparation of this research report, attests to the following: (1) that the views and opinions rendered in this research report reflect his or her personal views about the subject companies or issuers and (2) that no part of the research analyst's compensation was, is, or will be directly or indirectly related to the specific recommendations or views in this research report. In addition, said analyst(s) has not received compensation from any subject company in the last 12 months.

RAYMOND JAMES RELATIONSHIP DISCLOSURES
Certain affiliates of the RJ Group expect to receive or intend to seek compensation for investment banking services from all companies under research coverage within the next three months.

Raymond James & Associates, Inc. makes a market in the shares of Parsley Energy, Inc.

Additional Risk and Disclosure information, as well as more information on the Raymond James rating system and suitability categories, is available here.