Nearly all of the assaults occurred on Thursday along the border of South Williamsburg and Bedford-Stuyvesant between 9:55 and 11:15 a.m., cops said.

Three Minnesota teens have been arrested in connection with a video that shows a woman getting kicked in the head, the latest in a series of incidents targeting Asian Americans since the start of the coronavirus pandemic.

Earth is heating up at a much higher level than expected.

Earth is heating up at a much higher level than expected.

Three Minnesota teens have been arrested in connection with a video that shows a woman getting kicked in the head, the latest in a series of incidents targeting Asian Americans since the start of the coronavirus pandemic.

The gloves — and filters — are off.

With regulations urging residents statewide to wear masks when in public, here is how Southern Utahsn have fared following the recommended guidelines

Unilever is recalling Wall’s Mini Calippo (Orange and Lemon-Lime) multi-packs because they may contain small pieces of metal. The presence of metal makes this product unsafe to eat.

Markus Bailey missed most of his senior season with a knee injury but he started 40 games for the Boilermakers.

Valparaiso Vikings were set up for a tournament run before defection of star guard, who has offers from IU and Purdue.

The road to Indianapolis: Why Colts fell in love with Michael Pittman Jr.

Long-time Slashdot reader blackbearnh writes: Since the COVID-19 pandemic has made Personal Protective Equipment worth it's weight in gold, Makers have been trying to help bridge the gap. While sewn masks have been the most common solution, the 3D printing community has been pitching in as well. The Montana Mask has been one of the most popular designs... Thursday, the group Make the Masks announced that the design files and STLs to print the mask have been released under the GNU General Public License v3, allowing anyone to print, sell, remix or improve the design, as long as they conform to the license. Importantly, the GPLv3 includes an international non-exclusive patent grant, meaning that even if the inventors decide to apply for a patent, it will not restrict anyone from using the design.

Read more of this story at Slashdot.

The road to Indianapolis: Why Colts fell in love with Michael Pittman Jr.

Deaths and hospitalizations related to COVID-19 could increase dramatically in Montreal if social distancing measures are lifted, according to a new study.

Police Federation calls for anyone using coronavirus as a weapon automatically to be remanded in custody.

She created Mother's Day out of love for her own mother but was shocked by how it became commercialised.

As the UK looks to lift restrictions, how do other European countries plan to re-open after lockdown?

The government says it is "stepping up action" to stop the "totally unacceptable" crossings.

Alberta reported 59 new COVID-19 cases on Saturday, bringing the total number of active cases in the province to 1.837.

SuperSport United winger Aubrey Modiba says a conversation with club CEO Stanley Matthews convinced him to stay despite interest from Mamelodi Sundowns.

After six weeks of negotiating, the UN Security Council was close to agreeing on a resolution for a global ceasefire during the Covid-19 pandemic. Seems fair, right? Let's agree to stop killing each other for a while, so we can focus on the virus that's killing us instead?

China proposed that the text explicitly mention a commitment by member nations to support the efforts of the World Health Organization — who Donald Trump has blamed (without evidence) for withholding information on the coronavirus outbreak.

So the US looked at the resolution and said "LOL no," despite last minute efforts to reach a compromise. As The Guardian reports:

On Thursday night, French diplomats thought they had engineered a compromise in which the resolution would mention UN “specialized health agencies” (an indirect, if clear, reference to the WHO).

The Russian mission signaled that it wanted a clause calling for the lifting of sanctions that affected the delivery of medical supplies, a reference to US punitive measures imposed on Iran and Venezuela. However, most security council diplomats believed Moscow would withdraw the objection or abstain in a vote rather than risk isolation as the sole veto on the ceasefire resolution.

While everyone else seemed game to go along with these compromises, the US insisted it was one big Chinese trick. As one diplomat told CNN:  "This discussion has been taken hostage by issues that do not have to do with the real issues at stake. Instead it has been transformed into a fight between the US and China. Read the rest

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The post REPORT: Three Times Joe Biden Was Reportedly Involved with the Russia Collusion Hoax appeared first on Dennis Michael Lynch.

The city says couples can book its Helena Gutteridge Plaza at City Hall for just $85 and bring eight guests to have an outdoor, physically distant wedding ceremony.

18 June 2014

Members Event

Event participants

Professor Joseph S Nye, University Distinguished Service Professor, Harvard Kennedy School of Government
Chair: Gideon Rachman, Chief Foreign Affairs Commentator, Financial Times

geoffiths posted a photo:

Challenge #16 twist something up yourself. Or think of something that is mentally/socially twisted.

Hidden in the flash. posted a photo:

With a lockdown in place it is against the rules for me to go to places I like to shoot, so I though I would try to create a series called Isolated creativity. The series is not intended to be a diary but a way of documenting thoughts and emotions via photography.

I've felt a bit like Pink Rabbit over the passed few day. I'm not fed up and depressed by the lockdown but by the people who think that it's okay to break the rules. By the tabloid media that run stories that convince people it's okay to go out and about, when it Isn't. By the political points scoring that has started to appear in all forms of media.Lastly I fed up with second home owners that have turned up during lockdown and appear to be going out and about most days.

Just like Pink Rabbit I have the blues.

The investigation into the fatal shooting in Brunswick, Georgia, will also look at a neighbor of suspects Gregory and Travis McMichael who recorded video of the incident, authorities said.

Ousted vaccine expert Rick Bright said he is "frustrated at our inability to be heard as scientists."

Nepal protested India's inauguration of a new road to China that passes through territory claimed by Kathmandu on Saturday, with police arresting dozens demonstrating close to India's embassy. Indian defence minister Rajnath Singh on Friday inaugurated via video link the 80-kilometre (50 miles) long road from Ghatiabagarh in northern Uttarakhand state to the Lipu Lekh pass high in the Himalaya. The pass is claimed by Nepal based on an 1816 treaty that defines its western border with India.

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

β-Glucocerebrosidase (GBA) hydrolyzes glucosylceramide (GlcCer) to generate ceramide. Previously, we demonstrated that lysosomal GBA1 and nonlysosomal GBA2 possess not only GlcCer hydrolase activity, but also transglucosylation activity to transfer the glucose residue from GlcCer to cholesterol to form β-cholesterylglucoside (β-GlcChol) in vitro. β-GlcChol is a member of sterylglycosides present in diverse species. How GBA1 and GBA2 mediate β-GlcChol metabolism in the brain is unknown. Here, we purified and characterized sterylglycosides from rodent and fish brains. Although glucose is thought to be the sole carbohydrate component of sterylglycosides in vertebrates, structural analysis of rat brain sterylglycosides revealed the presence of galactosylated cholesterol (β-GalChol), in addition to β-GlcChol. Analyses of brain tissues from GBA2-deficient mice and GBA1- and/or GBA2-deficient Japanese rice fish (Oryzias latipes) revealed that GBA1 and GBA2 are responsible for β-GlcChol degradation and formation, respectively, and that both GBA1 and GBA2 are responsible for β-GalChol formation. Liquid chromatography–tandem MS revealed that β-GlcChol and β-GalChol are present throughout development from embryo to adult in the mouse brain. We found that β-GalChol expression depends on galactosylceramide (GalCer), and developmental onset of β-GalChol biosynthesis appeared to be during myelination. We also found that β-GlcChol and β-GalChol are secreted from neurons and glial cells in association with exosomes. In vitro enzyme assays confirmed that GBA1 and GBA2 have transgalactosylation activity to transfer the galactose residue from GalCer to cholesterol to form β-GalChol. This is the first report of the existence of β-GalChol in vertebrates and how β-GlcChol and β-GalChol are formed in the brain.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Immigration is a prominent part of the United States’ DNA, despite concerns about immigrants’ ability to integrate. An examination of recent immigrant inflows shows newcomers to the United States are integrating well, based on language proficiency, socioeconomic attainment, political participation, residential locale, and social interaction indicators.

Two competing models for selecting economic-stream immigrants are now prevalent in advanced industrialized economies: points-based and employer-led selection. Increasingly, however, hybrid selection systems are being created, implementing best practices from each selection process.

The report examines U.S. immigration and international development policies, which have unique objectives and respond to distinct political and administrative constraints, and points out that international development has never been a U.S. immigration policy objective; nonetheless, it is an unintended consequence.

While aspects of the U.S. immigration system facilitate newcomers’ contributions to economic growth and competitiveness, others undermine them. Reforms are needed to enhance the job-creating power of U.S. employers and strengthen the system’s ability to select effectively from the large pool of foreign workers.

This report explores the migration patterns and demographics of Black African immigrants in the United States, examining their admission channels, human-capital characteristics, and labor market performance. The authors also provide an analysis of these immigrants' integration prospects.

Since 1970, the immigrant populations from Mexico and Central America living in the United States have increased significantly: rising by a factor of 20 even as the total U.S. immigrant population increased four-fold over the period. This demographic report examines the age, educational, and workforce characteristics of these immigrants.

Showcasing joint research by MPI and the European University Institute and funded by the European Commission, this event featured discussion on some of the most promising reform proposals on both sides of the Atlantic. Speakers discuss the project’s comparative research, which draws on MPI’s longstanding experience advising European and North American governments on immigration.

European dominance in U.S. immigration flows has decreased significantly since World War II, a result of economic, demographic, and policy trends on both sides of the Atlantic. Today, migration from European Union Member States to the United States, while small, is characterized by a substantial numbers of European scientists, professionals, and businesspeople.

The United States has historically offered unparalleled economic opportunity to successive generations of immigrants and their children, poised to play an increasing role in the U.S. economy. But the lasting impact of job loss and slower growth over the next decade will translate into fewer opportunities for workers—and immigrants may prove the most vulnerable.

MANCHESTER, England (AP): Paul Pogba and Marcus Rashford are expected to be available for Manchester United whenever the Premier League is allowed to resume after the suspension caused by the coronavirus outbreak. Whether United manager Ole Gunnar...

Immigration from the Caribbean to the United States is a relatively recent phenomenon, beginning largely after 1965. This report provides a demographic profile of the 1.7 million Caribbean immigrants in the United States: their geographic settlement, education and workforce characteristics, earnings, modes of entry, and more.

African immigrants generally fare well on integration indicators, with college completion rates that greatly exceed those for most other immigrant groups and U.S. natives, this report finds. The United States, Canada, and Australia disproportionally attract better-educated African migrants then do the United Kingdom, France, and other European countries.

This report focuses on the development of children of Black immigrants in the United States, comparing against the outcomes for their peers in native-born and other immigrant families. It also compares these U.S. children to those in the United Kingdom, where there is a large Black immigrant population but a notably different policy context of reception.

From 1980 to 2013, the sub-Saharan African immigrant population in the United States increased from 130,000 to 1.5 million, roughly doubling each decade between 1980 and 2010. This profile provides up-to-date demographic information for sub-Saharan immigrants including location, educational attainment, workforce participation, and much more.