Republicano perdeu seu principal discurso, os bons números econômicos, seis meses antes da eleição. Agora, luta para ser visto como bom gestor da crise.

Austin Burton announced last week he's transferring from UCLA to Purdue. He'll be a graduate transfer with two years of eligibility.

The Duke of Sussex sent a special video message to mark what would have been the opening ceremony of the 2020 Invictus Games.

In the latest MOTD podcast the experts discussed the top 10 Ballon d'Or winners of the Premier League era - and here's how you ranked them.

Filed under: Food and Drink, North America, United States

• Regardless of the area's suitability for growing grapes, plop down a vineyard or winery and travelers will come for a taste and buy a sympathy bottle (pro tip: go for the ice wine as it's harder to mess up)
• Ski resorts looking to attract off-season dollars or stale attractions looking to draw media coverage and visitors hook up a zipline

Continue reading Vineyard and Zipline Trends Collide at the Pinot Express

Vineyard and Zipline Trends Collide at the Pinot Express originally appeared on Gadling on Wed, 11 Dec 2013 16:54:00 EST. Please see our terms for use of feeds.

Hairdressers have been forced to go underground as lockdown regulations stifle their livelihood and job security. For some, bootlegging has been 'life-saving'.

A man is dead after a single-vehicle motorcycle crash in southeast Calgary.

Mercedes' Lewis Hamilton has said that holding races without any fans will feel "even worse than a test day".

Former New Zealand head coach and current England defence chief John Mitchell believes some good may come for rugby union from the coronavirus.

Fear is ripe soil for the unscrupulous. With so much uncertainty and concern over our health and the broader world economy, cybercriminals have been playing on that fear to steal a few extra dollars out of the most scared and vulnerable. The U.S. Secret Service warned that phishing attacks were up significantly and scams over fake COVID-19 treatments have led to seizures and arrests.

From companies and organizations to individuals, it’s never been more important for everyone to have their cybersecurity measures on high alert. And whether you’re looking to protect your own assets or you’ve been tasked with safeguarding a company and all its workers, the vital work of white hat hackers is absolutely essential these days.

The training in The Ultimate 2020 White Hat Hacker Certification Bundle can put you in a position to understand all aspects of maintaining cybersecurity for a communication system of virtually any size, a lucrative career that can earn you a six-figure income.

The four-part Complete Cyber Security Course (taught by cybersecurity expert and noted consultant Nathan House) is a 360-degree starting point for any cybersecurity career. Starting at the beginning, each part of this multi-pronged introduction will help guide you through vital knowledge, from network hacking techniques and vulnerability scanning to all the defense methods that assure every laptop, desktop, smartphone and tablet in your network remain secure.

The hacker training continues with the rest of the courses in the collection, including building security analysis tools using Python and learning how to analyze web app security vulnerabilities and solutions using frameworks like Ruby on Rails and PHP. Read the rest

The DML News App offers the best in news reporting.

The post REPORT: Here’s What Comey Told Congress About Whether Flynn Lied To FBI: Transcript appeared first on Dennis Michael Lynch.

As Quebec begins to reopen schools and businesses, Prime Minister Justin Trudeau said he’s ‘worried’ about the province’s deconfinement plans, particularly in Montreal.

Hospitals in British Columbia and Ontario are beginning to address major backlogs in surgeries after the COVID-19 pandemic forced thousands of cancellations that could take well over a year to address.

The following article, Mark Levin on Michael Flynn Bombshell Documents: This Is “Barack Obama’s Blue Dress” Without The DNA, was first published on 100PercentFedUp.com.

Mark Levin nailed the importance of the newly released documents that cleared Michael Flynn and pinned Obama to the wall. He says that the documents are “Barack Obama’s Blue Dress” without the “DNA” alluding to the blue dress from Monica Lewinsky that proved Bill Clinton’s guilt. Levin begins by praising AG Bill Barr and then […]

Continue reading: Mark Levin on Michael Flynn Bombshell Documents: This Is “Barack Obama’s Blue Dress” Without The DNA ...

The following article, Professor Who Mocked Barron Trump During Senate Hearings Gets Censorship Position at Facebook, was first published on 100PercentFedUp.com.

Facebook just announced a 20 person board of oversight that will assist with content moderation. One of the people selected for the board, a professor at Stanford Law School, was announced as a member of the board and is raising eyebrows because of her snarky comment about Barron Trump during Senate Impeachment Hearings. Pamela Karlan, […]

Continue reading: Professor Who Mocked Barron Trump During Senate Hearings Gets Censorship Position at Facebook ...

The following article, Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “, was first published on 100PercentFedUp.com.

Now that Michael Caputo, who was previously a target of the Mueller investigation, has been cleared, he has released some pretty damning text messages from the virulently anti-Trump lawmaker from California, Rep. Eric Swalwell (D). The text messages appear to prove that Swalwell, who has spent the last 3 1/2 years calling for Trump’s impeachment, […]

Continue reading: Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “ ...

Members Event

Event participants

Richard Heald, CEO, UK India Business Council (UKIBC)
Manoj Ladwa, Communications Director, Narendra Modi for Prime Minister Campaign
Dr Gareth Price, Senior Research Fellow, Asia Programme, Chatham House

Chair: Adam Roberts, South Asia Bureau Chief, Delhi, The Economist

Research Event

Event participants

Dr Doyin Okupe, Senior Special Assistant on Public Affairs to the President of Nigeria 

Research Event

Event participants

Jeremy Gordon, Director, China Business Services
Chair: Roderic Wye, Associate Fellow, Asia Programme, Chatham House

12 November 2014 , Volume 90, Number 6

23 February 2015

Elizabeth Spiers wrote about an incident where Jared Kushner used the memorial of an employee to congratulate himself for success.

Bacterial products such as lipopolysaccharides (or endotoxin) cause systemic inflammation, resulting in a substantial global health burden. The onset, progression, and resolution of the inflammatory response to endotoxin are usually tightly controlled to avoid chronic inflammation. Members of the NF-κB family of transcription factors are key drivers of inflammation that activate sets of genes in response to inflammatory signals. Such responses are typically short-lived and can be suppressed by proteins that act post-translationally, such as the SOCS (suppressor of cytokine signaling) family. Less is known about direct transcriptional regulation of these responses, however. Here, using a combination of in vitro approaches and in vivo animal models, we show that endotoxin treatment induced expression of the well-characterized transcriptional repressor Krüppel-like factor 3 (KLF3), which, in turn, directly repressed the expression of the NF-κB family member RELA/p65. We also observed that KLF3-deficient mice were hypersensitive to endotoxin and exhibited elevated levels of circulating Ly6C+ monocytes and macrophage-derived inflammatory cytokines. These findings reveal that KLF3 is a fundamental suppressor that operates as a feedback inhibitor of RELA/p65 and may be important in facilitating the resolution of inflammation.

Prevention of aberrant cutaneous wound repair and appropriate regeneration of an intact and functional integument require the coordinated timing of fibroblast and keratinocyte migration. Here, we identified a mechanism whereby opposing cell-specific motogenic functions of a multifunctional intracellular and extracellular protein, the receptor for hyaluronan-mediated motility (RHAMM), coordinates fibroblast and keratinocyte migration speed and ensures appropriate timing of excisional wound closure. We found that, unlike in WT mice, in Rhamm-null mice, keratinocyte migration initiates prematurely in the excisional wounds, resulting in wounds that have re-surfaced before the formation of normal granulation tissue, leading to a defective epidermal architecture. We also noted aberrant keratinocyte and fibroblast migration in the Rhamm-null mice, indicating that RHAMM suppresses keratinocyte motility but increases fibroblast motility. This cell context–dependent effect resulted from cell-specific regulation of extracellular signal-regulated kinase 1/2 (ERK1/2) activation and expression of a RHAMM target gene encoding matrix metalloprotease 9 (MMP-9). In fibroblasts, RHAMM promoted ERK1/2 activation and MMP-9 expression, whereas in keratinocytes, RHAMM suppressed these activities. In keratinocytes, loss of RHAMM function or expression promoted epidermal growth factor receptor–regulated MMP-9 expression via ERK1/2, which resulted in cleavage of the ectodomain of the RHAMM partner protein CD44 and thereby increased keratinocyte motility. These results identify RHAMM as a key factor that integrates the timing of wound repair by controlling cell migration.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

Endorepellin, the C-terminal fragment of the heparan sulfate proteoglycan perlecan, influences various signaling pathways in endothelial cells by binding to VEGFR2. In this study, we discovered that soluble endorepellin activates the canonical stress signaling pathway consisting of PERK, eIF2α, ATF4, and GADD45α. Specifically, endorepellin evoked transient activation of VEGFR2, which, in turn, phosphorylated PERK at Thr980. Subsequently, PERK phosphorylated eIF2α at Ser51, upregulating its downstream effector proteins ATF4 and GADD45α. RNAi-mediated knockdown of PERK or eIF2α abrogated the endorepellin-mediated up-regulation of GADD45α, the ultimate effector protein of this stress signaling cascade. To functionally validate these findings, we utilized an ex vivo model of angiogenesis. Exposure of the aortic rings embedded in 3D fibrillar collagen to recombinant endorepellin for 2–4 h activated PERK and induced GADD45α vis à vis vehicle-treated counterparts. Similar effects were obtained with the established cellular stress inducer tunicamycin. Notably, chronic exposure of aortic rings to endorepellin for 7–9 days markedly suppressed vessel sprouting, an angiostatic effect that was rescued by blocking PERK kinase activity. Our findings unravel a mechanism by which an extracellular matrix protein evokes stress signaling in endothelial cells, which leads to angiostasis.

While aspects of the U.S. immigration system facilitate newcomers’ contributions to economic growth and competitiveness, others undermine them. Reforms are needed to enhance the job-creating power of U.S. employers and strengthen the system’s ability to select effectively from the large pool of foreign workers.

Showcasing joint research by MPI and the European University Institute and funded by the European Commission, this event featured discussion on some of the most promising reform proposals on both sides of the Atlantic. Speakers discuss the project’s comparative research, which draws on MPI’s longstanding experience advising European and North American governments on immigration.

At least 10 stores inside the Portmore Mall have fallen casualty to the economic chokehold brought on by the COVID-19 pandemic, with several others struggling to stay afloat as St Catherine businesses grapple with revenue losses. And with a 14-day...

This report draws on a six-year longitudinal study of Palm Beach County, FL, examining parenting, child care enrollment, and other factors that encourage early school success. The authors find kindergarten-age children of Black immigrants have significantly higher odds of being ready for school than children of Latina immigrant or Black U.S.-born mothers.

Using a nationally representative U.S. birth-cohort study, this report examines levels of school readiness among young children by race/ethnicity and nativity. The authors identify the contextual factors — such as family circumstances, parenting practices, and enrollment in center-based child care — that encourage early school success.

ADVISORY COLUMN: SMALL BUSINESS On Thursday, May 7, the RJR/GLEANER Communications group staged a virtual town hall meeting on Television Jamaica titled “COVID-19...

RISKS...

Uber lost US$2.9 billion in the first quarter as its overseas investments were hammered by the coronavirus pandemic, but the company is looking to its growing food delivery business and aggressive cost-cutting to ease the pain. The ride-hailing...

Recent calls by the Jamaica Manufacturing and Exporters Association, JMEA, for more targeted concessions on raw materials that are processed for re-export sent Jamaica Agricultural Commodities Regulatory Authority, JACRA, back to the drawing board...

Lady Allen – wife of Jamaica’s Governor General Sir Patrick Allen – says Jamaican women are among the strongest and most resilient in the world, and despite many bearing the full brunt of the coronavirus pandemic as breadwinners for their families...

This book reflects the effort of the Transatlantic Council on Migration to map how profound demographic change is likely to affect the size and character of global migration flows; and how governments can shape immigration policy in a world increasingly attuned to the hunt for talent.

This edited volume addresses the impact of the economic crisis in seven major immigrant-receiving countries: the United States, Germany, Ireland, Portugal, Spain, Sweden, and the United Kingdom. 

This edited volume showcases approaches toward border management in Europe, Central America, and North America, and reflects on the challenges that countries in these regions face in managing their borders. The book brings together perspectives from both sides of the Atlantic on what border security means in practice, the challenges that continue to evade policymakers, and what policies have been the most (and least) successful in achieving “secure” borders.

The enactment of President Clinton’s Limited English Proficiency (LEP) Executive Order, issued in 2000, triggered a proliferation of efforts to provide services to individuals who cannot speak, understand, read, or write English fluently. With increased service provision, state and local government agencies have expressed a strong and growing interest in assuring the quality and cost-effectiveness of language access services. This paper attempts to catalog and describe some of those tools and practices.

This report provides an overview of several commonly used translation and interpretation technologies. It aims to assist language access practitioners in understanding and identifying which systems would best meet their agency’s language access needs.

This interactive language access webinar, one in a series offered by the Migration Policy Institute's National Center on Immigrant Integration Policy, examines how New York and Illinois have broken down some of these barriers to proactively engage LEP communities to obtain workforce services.

This webinar examines how New York and Illinois have proactively engaged Limited English Proficient (LEP) communities to obtain workforce services.

In this webinar, experts discuss barriers immigrant and LEP individuals face in accessing the WIA system, how a revitalized WIA could address these barriers, and the extent to which the current Senate Health, Education, Labor, and Pensions (HELP) Committee's WIA reauthorization proposal addresses these barriers.

This is the latest in NCIIP’s language access webinar series exploring the policy and program implementation imperatives for government and community agencies serving Limited English Proficient (LEP) populations.

This webinar from the MPI’s National Center on Immigrant Integration Policy and the U.S. Conference of Catholic Bishops’ Bridging Refugee Youth & Children’s Services program explores federal requirements for providing interpretation and translation in schools and how select school districts in Minnesota and Colorado have managed these requirements.