Independent MHA Eddie Joyce is suing four people, including the premier, for defamation over the handling of a harassment complaint that saw him turfed from the Liberal cabinet and caucus in 2018.

A man is in police custody following an incident that briefly put the Burin Peninsula community of Lawn on high alert on Friday morning. 

Long-term care homes in Eastern Health's jurisdiction are allowing for people to again visit their relatives by windows, just in time for Mother's Day. 

Green Depots will begin to reopen across the province on Monday, according to the Multi-Materials Stewardship Board, though dates and hours of operation may vary between sites. 

Demand for service is not expected to recover in the coming weeks, says Marine Atlantic.

The RCMP say criminal charges are pending after a report came in about an arson Friday morning, in which everyone inside was able to escape with no injuries.

Every day around the province the Here and Now team pull out all the stops to cover your news and weather

The province is still on track to move to a lower alert level Monday, which would ease some of the public health measures in place aimed at curbing COVID-19.

64-year-old John Neville, in agony and needing his wisdom tooth extracted, has been navigating a complex tangle of COVID-19 restrictions in an unsuccessful attempt at getting emergency dental care.

Philip Pynn is being released from federal prison for a second time, after breaching conditions landed him back behind bars last year — but it's not clear where he can live.

The COVID-19 briefings started out as an urgent response to a public health crisis. As John Gushue writes, Premier Dwight Ball has grown comfortable in the role of hosting a program focused on government programs.

COVID-19 is not an equal opportunity pandemic. As Memorial president Vianne Timmons writes in this guest column, women are often in harm's way because of their work.

Staff halted $100 service over pandemic concerns, forcing homeowners to private plumbers much more, and Danny Breen is urging them to contact the city.

There are 14 active cases of COVID-19 remaining in the province.

"If you are starting out on the language and culture learning journey or if you have been at it a while and are feeling tired and despondent, I encourage you to persevere. To stick with it and pray for strength to continue. Keep asking questions and keep learning about the culture," urges Beth.

A South East Asian OM worker shared about how her relationship with Jesus has changed her life from one of fear to leading others and modeling Christ's love.

Noy shares her journey of experiencing God's love for herself and forgiving the community that persecuted her family.

Service Team members share why they enjoy being onsite during Setup Week, when the vision of TeenStreet becomes a reality for them, and what part of the upcoming conference they are most excited for.

God uses Janet to reach people through one-on-one encounters at a bookshop in a closed country.

The Main Hall session focused on wisdom, a word and concept used very much in everyday life. The truth is that God gives us wisdom, and He invites us to desire and search for it as we would search for treasure.

On Day 5, we looked at 'Power.' Our words have the power to hurt and destroy as well as encourage and build up. Jesus' words give joy, life, peace, hope and purpose to those who believe Him.

For Roberto Ramirez, serving God in missions has been one step of faith after another.

Tinashe disciples and shows Zimbabweans that they, too, are called to serve; everyone can share their own stories with others to show the love of Christ. 

Mar del Plata, Argentina :: A church congregation learns about nations without access to the gospel.

Mar del Plata, Argentinien :: Eine Gemeinde hört von Ländern, die keinen Zugang zum Evangelium haben

Mar del Plata, Argentina :: Crew work alongside a pastor who has plans to improve children's futures.

In einer Kleinstadt im Kaukasus finden Kinder neue Hoffnung.

When Elizabeth May, parliamentary leader of the federal Green Party, proclaimed on Tuesday that "oil is dead," she was correct in a philosophical sense. But not in a practical, real world sense.

Take a listen to Episode 9 of CBC Edmonton's new podcast, The Loop.

Residents are cleaning up damage to hundreds of homes and businesses caused by floods in the northern Alberta hamlet of Fort Vermilion.

A longstanding battle between an Alberta entrepreneur and a $50-million lottery winner is still raging after a new legal judgment, a securities investigation, allegations of harassment and even duelling ebooks.

In a new twist on oral history, University of Alberta paleontologists have discovered that an Argentinian reptile from 95 million years ago developed a type of tooth enamel that is common in humans and other mammals but rare among reptiles.

The NDP health critic says comments Premier Doug Ford made this week calling out medical officers of health for not getting enough COVID-19 testing done are uncalled for.

Before COVID-19 hit, Hemliss Eloïse Konan had plans for how she'd spend her summer in Sudbury. After finishing her first year at Laurentian University, Konan planned to stay in residence, and get a job for the summer.

A new study facilitated by the Workplace Safety and Insurance Board of Ontario states miners who were forced to inhale an aluminum powder were found to have a higher risk of Parkinson’s disease.

As the province of Ontario starts to relax some COVID-19 restrictions, one Sudbury business owner says it feels great to be opening back up.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

A former miner who was forced to breathe in a fine aluminum dust before each shift says he’s not surprised a new report links that dust and a higher risk of Parkinson’s disease.

Those in Sudbury may have had a few choice words when waking up Friday morning after a snowfall overnight.

Provincial surveillance testing has returned a positive case of COVID-19 in a resident of Wikwemikong Nursing Home on Manitoulin Island. Ogimaa Duke Peltier says every staff member and resident underwent tests Tuesday and Wednesday of this week and the results are starting to come in.

The head of a Sudbury-based book publishing company says she’s shifting launches online due to COVID-19.

The following is a guest post by Martha H. Kennedy, Curator of Popular & Applied Graphic Art, Prints & Photographs Division. During Will Eisner Week, March 1-7, 2020, the Library of Congress joins art schools, libraries, universities, and museums in a global celebration of this legendary creator’s contributions to the world of comic art and […]

Spring has arrived. While some of us may have an opportunity to carefully step outside and view blossoms in our own neighborhood, others may not. Wherever you are, you can take a virtual stroll among the shoots and blossoms planted among the collections of the Prints & Photographs Division. Many images of gardens can be […]

The following is a guest post by Katherine Blood, Curator of Fine Prints, Prints & Photographs Division. As the Library of Congress marks its 220th year of serving the nation, the publication of a new guide tells two stories: how staff have for decades worked with art professionals to build the collections and how by […]

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H₂O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.