ted velocityconf: RT @suzaxtell: #WomeninTech You're invited to a women's meetup on Tues May 28 in SF w/ @courtneynash @mjawili, more http://t.co/MsMZ0IK8L2 By feedproxy.google.com Published On :: Tue, 21 May 2013 20:27:27 +0000 velocityconf: RT @suzaxtell: #WomeninTech You're invited to a women's meetup on Tues May 28 in SF w/ @courtneynash @mjawili, more http://t.co/MsMZ0IK8L2 Full Article
ted News24.com | Suspected zama zamas arrested while allegedly processing gold in Limpopo By www.news24.com Published On :: Sat, 09 May 2020 12:47:47 +0200 Eight suspected illegal miners, known as zama zamas, have been arrested in Limpopo, following a Hawks investigation. Full Article
ted News24.com | UPDATED: CORONAVIRUS FAQs | All your questions answered By www.news24.com Published On :: Wed, 06 May 2020 06:36:15 +0200 News24 answers some of the most frequently asked questions on coronavirus and level 4 of the lockdown in South Africa. Full Article
ted Channel24.co.za | SABC granted a local TV content quota reprieve due to Covid-19 By www.channel24.co.za Published On :: Thu, 07 May 2020 10:10:37 +0200 South Africa's broadcasting regulator has approved an application from the SABC for its annual local TV content quotas to be waived because of the Covid-19 coronavirus pandemic. Full Article
ted News24.com | Man arrested for spate of lockdown robberies in Tshwane, 'accomplices' killed in shootout with cops By www.news24.com Published On :: Sat, 09 May 2020 11:25:51 +0200 An alleged robber, who was wounded while his two suspected accomplices were killed in a shootout with police, has been arrested. Full Article
ted Tips for Getting Started with a Business Growth Framework By feedproxy.google.com Published On :: Tue, 26 Mar 2019 00:00:00 +0000 Growth is top of mind for most companies, but it can be tricky to get started with developing a growth framework. For one thing, most companies aren’t organized around growth, so thinking about growth requires a lot of collaboration between teams. Additionally, companies often don’t have easy access to all of the data they need to understand growth. Full Article
ted SBA Disaster Loans Capped at $150K, Limited to Agriculture By feedproxy.google.com Published On :: Fri, 08 May 2020 00:00:00 -0500 The Economic Injury Disaster Loan program is now limiting both the amount and recipients it will consider for the emergency loans. Full Article
ted AT#226 - Travel to Island of Maui, Hawaii revisited part 1 By traffic.libsyn.com Published On :: Sat, 03 Apr 2010 18:10:00 +0000 The Amateur Traveler talks about a recent trip to Maui as the guest of the Maui Convention and Visitors Bureau. In this first half of a two part episode Chris gives an overview of the island and talks about the Hawai’i Nature Center, the beaches and the road to Hana. Learn about swimming or hiking in lava tubes, hidden beaches and some of the naive plants of Maui.Show NotesVisit Maui BlogRainfall map of MauiWailea Beach Marriott Resort & SpaThe Ritz-Carlton, KapaluaKamehameha IHawaiian LanguageBeaches: * Makena (big beach) * Chang’s Beach * ‘Ahihi Bay * Maluaka Beach * Wai’ anapanapa State Park * Ka’eleku Caverns Full Article
ted AT#226 - Travel to the Island of Maui, Hawaii revisited part 2 By pacific.amateurtraveler.com Published On :: Thu, 08 Apr 2010 07:13:45 +0000 The Amateur Traveler talks about a recent trip to Maui as the guest of the Maui Convention and Visitors Bureau. In this second half of a two part episode Chris talks about some of the local culture, food and festivals. He had a chance to explore his rancher roots at one of Maui’s cattle ranches, dine on local produce at some high end restaurants, mix with the locals at the Maui Ag Festival and learn more of Maui’s culture at the Celebration of the Arts. Full Article
ted AT#513 - Travel to the United Arab Emirates By middleeast.amateurtraveler.com Published On :: Sat, 07 May 2016 11:00:00 +0000 Hear about travel to United Arab Emirates as the Amateur Traveler talks to travel writer Zora O'Neill about her recent trip to these small states on the Persian / Arabian Gulf. Full Article
ted AT#480 - United States UNESCO World Heritage Sites (replay) By usa.amateurtraveler.com Published On :: Sat, 23 Dec 2017 15:00:00 +0000 Hear about the United States UNESCO World Heritage Sites as the Amateur Traveler talks to Gary Arndt from Everything-Everywhere.com and The Global Travel Conspiracy podcast about these best of the best sites. Full Article
ted AT#646 - Travel to the United Arab Emirates (UAE) By amateurtraveler.com Published On :: Sat, 23 Feb 2019 15:00:00 +0000 Hear about travel to the United Arab Emirates (UAE) as the Amateur Traveler talks to Keri Hedrick from ourglobetrotters.com and familytravel-middleeast.com about her adopted country. Full Article
ted 32 Years After Teen’s Body Found Strangled on Side of Road, Suspect Finally Arrested By www.westernjournal.com Published On :: Fri, 08 May 2020 21:12:25 +0000 After three decades of mystery, the cold case murder of a 17-year-old Ohio teen named Barbara Blatnik has been solved thanks in part to DNA genealogical research. The last time anyone saw Blatnik alive was around midnight on Dec. 19, 1987, when a friend dropped her off in Garfield Heights, Ohio. The following day, Blatnik,… The post 32 Years After Teen’s Body Found Strangled on Side of Road, Suspect Finally Arrested appeared first on The Western Journal. Full Article Lifestyle crime Justice murder Ohio police Rape sexual assault
ted Giant Asian ‘Murder Hornets’ Spotted in US for First Time By www.westernjournal.com Published On :: Sat, 09 May 2020 15:16:55 +0000 What one expert called “something out a monster cartoon” has now arrived in the United States. The Asian giant hornet, which can decimate bee colonies and is responsible for 50 deaths a year in Japan, is now in Washington state, according to The New York Times. A new threat reaches the United States: A massive… The post Giant Asian ‘Murder Hornets’ Spotted in US for First Time appeared first on The Western Journal. Full Article News Animals nature US News Washington State
ted Ted Cruz Stops by Shelly Luther’s Salon for a Trim, ‘True Authenticity’ Leaves Her Sobbing By www.westernjournal.com Published On :: Sat, 09 May 2020 17:26:32 +0000 Sen. Ted Cruz was apparently about to get into mullet territory. He hadn’t had a haircut in three months. Things were looking grim for the Texas senator. Thankfully, Shelley Luther is able to do something about that. Luther is both famous and free after a viral clip of her standing up to a judge who… The post Ted Cruz Stops by Shelly Luther’s Salon for a Trim, ‘True Authenticity’ Leaves Her Sobbing appeared first on The Western Journal. Full Article Commentary Court Dallas Greg Abbott Heidi Cruz law Ted Cruz Texas
ted Has the U.S. Really Shifted on Deportations? By feedproxy.google.com Published On :: Tue, 20 Oct 2015 19:41:50 EST A year after the Obama administration changed its policy on which undocumented immigrants it would target for deportation, it's not clear who is being sent back. Full Article
ted 17 Indicted in Bust of $32 Million Online Gambling Ring By feedproxy.google.com Published On :: Wed, 28 Oct 2015 18:40:11 EST The online gambling ring allegedly used an offshore website to help book $32 million in illegal sports wagers placed by more than 2,000 bettors in the United States. Full Article
ted It’s an unprecedented crisis: 8 things to do right now By feedproxy.google.com Published On :: Wed, 18 Mar 2020 10:00:00 +0000 Even with a stellar crisis plan, the COVID-19 pandemic presents a set of challenges unprecedented in our lifetimes. We don’t know what’s going to happen, and we’re dealing with something growing exponentially, creating uncertainty on a global scale. I managed a team of 40 in Singapore during SARS. That crisis was different, hitting Singapore and […] Full Article Future of the Firm Deep Dive
ted Boil water advisory may be lifted sooner than anticipated: RM of Wood Buffalo By edmonton.ctvnews.ca Published On :: Sat, 9 May 2020 11:13:29 -0600 The flood stricken Regional Municipality of Wood Buffalo says it is on track to lift its boil water advisory sooner than it was originally projected. Full Article
ted 2 Morley, Alta. residents arrested following drug and firearm seizure By calgary.ctvnews.ca Published On :: Sat, 9 May 2020 09:18:00 -0600 Cochrane RCMP say charges have been laid against two individuals in connection with an investigation that began with a firearms complaint. Full Article
ted Sport24.co.za | Nadal believes Djokovic will have to be vaccinated By www.sport24.co.za Published On :: Sat, 09 May 2020 12:28:07 +0200 Rafael Nadal insists all players, including Novak Djokovic, will need to take a coronavirus vaccine should one become available. Full Article
ted Sport24.co.za | Man United turned to Igalo after Rondon move was blocked By www.sport24.co.za Published On :: Sat, 09 May 2020 16:25:43 +0200 Salomon Rondon says he was on his way to joining Manchester United in January only for Dalian Professional to block the move. Full Article
ted Sport24.co.za | Jordaan admits PSL decision is complicated By www.sport24.co.za Published On :: Sat, 09 May 2020 13:22:00 +0200 Danny Jordaan says it would be massively complicated to ensure the safety of supporters should the PSL return while Covid-19 is still prevalent in South Africa. Full Article
ted How to Find Online Casino Bonuses Suited for You By feedproxy.google.com Published On :: Fri, 01 May 2020 13:55:28 +0000 Online casinos are famous for their bonuses and promotions. These offers are one of the key advantages that online casinos hold over their land-based equivalents. But there are a lot of different types of online casino bonuses. Anyone who is quite new to gambling online might be confused about how they work in practice. As […] The post How to Find Online Casino Bonuses Suited for You appeared first on Dumb Little Man. Full Article How To Money
ted Interested in Building a Food Delivery App? By feedproxy.google.com Published On :: Tue, 05 May 2020 11:30:11 +0000 Today, there is a mobile application for every task we do daily. For this reason, the mobile app industry has witnessed enormous growth over the last couple of years, with billions of apps downloaded every year. Businesses of all kinds have certainly benefited from the emergence of mobile applications. One of the apps that’s seen […] The post Interested in Building a Food Delivery App? appeared first on Dumb Little Man. Full Article Food Mobile Technology
ted Newsroom: US Spotify Listeners Surpassed Pandora Listeners in 2019, Sooner than Expected By www.emarketer.com Published On :: Tue, 25 Feb 2020 04:01:00 GMT Spotify will gain more than 10 million US listeners in 2020   February 25, 2020 (New York, NY)Â – Pandora is no longer the most popular music streaming service in the […] Full Article
ted Woman, 70, airlifted in serious condition after car collides with transport truck in Huntsville By barrie.ctvnews.ca Published On :: Thu, 7 May 2020 12:35:00 -0400 Full Article
ted 'Everybody around here is dying,' Shelburne retirement home devastated by outbreak By barrie.ctvnews.ca Published On :: Thu, 7 May 2020 17:17:00 -0400 The Shelburne Retirement Residence has been devastated by an outbreak of COVID-19, with 90 per cent of its residents becoming infected. Full Article
ted BREAKING: Sen Marsha Blackburn Introduces Stop COVID Act…Allowing US Citizens To Sue Communist China For Damage They’ve Inflicted On Our Nation By 100percentfedup.com Published On :: Wed, 06 May 2020 16:56:18 +0000 The following article, BREAKING: Sen Marsha Blackburn Introduces Stop COVID Act…Allowing US Citizens To Sue Communist China For Damage They’ve Inflicted On Our Nation, was first published on 100PercentFedUp.com. Yesterday, Senator Marsha Blackburn (R-TN), along with Senator Martha McSally (R-AZ) introduced the Stop COVID Act, giving Americans the ability to sue Communist China for the damage they’ve inflicted on our nation. Senator Blackburn appeared on Fox News with host Judge Jeanine where she explained the act to Jeanine Pirro. Blackburn told the Fox News […] Continue reading: BREAKING: Sen Marsha Blackburn Introduces Stop COVID Act…Allowing US Citizens To Sue Communist China For Damage They’ve Inflicted On Our Nation ... Full Article Featured Politics
ted BREAKING: MI Lawmakers File Lawsuit Challenging Governor’s “Improper” and “Invalid” Emergency Orders: “We’ve attempted to partner with our governor, but she’s rejected” By 100percentfedup.com Published On :: Wed, 06 May 2020 20:54:20 +0000 The following article, BREAKING: MI Lawmakers File Lawsuit Challenging Governor’s “Improper” and “Invalid” Emergency Orders: “We’ve attempted to partner with our governor, but she’s rejected”, was first published on 100PercentFedUp.com. In addition to crushing Michigan's economy, the governor's reckless, one-size-fits-all executive orders are harming an untold number of Michigan citizens. Continue reading: BREAKING: MI Lawmakers File Lawsuit Challenging Governor’s “Improper” and “Invalid” Emergency Orders: “We’ve attempted to partner with our governor, but she’s rejected” ... Full Article Featured Politics
ted NY Shame: Workers Who Tested Positive For COVID-19 Were Allowed To Remain On The Job At Nursing Homes, As Death Toll For Nursing Home Patients Exceeds 3,000 By 100percentfedup.com Published On :: Wed, 06 May 2020 21:44:54 +0000 The following article, NY Shame: Workers Who Tested Positive For COVID-19 Were Allowed To Remain On The Job At Nursing Homes, As Death Toll For Nursing Home Patients Exceeds 3,000, was first published on 100PercentFedUp.com. The coronavirus crisis at New York’s nursing homes is even worse than previously thought. Monday night, the state Department of Health issued new data, adding more than 1,600 people who were presumed to have died of the virus in nursing homes, but did not have a confirmed diagnosis, to the official toll. As of May […] Continue reading: NY Shame: Workers Who Tested Positive For COVID-19 Were Allowed To Remain On The Job At Nursing Homes, As Death Toll For Nursing Home Patients Exceeds 3,000 ... Full Article Featured Politics
ted BREAKING: Ex-Cop Father and Son Arrested and Charged With Murder of Black Man Jogging In Neighborhood…President Trump Responds [VIDEO] By 100percentfedup.com Published On :: Fri, 08 May 2020 02:55:40 +0000 The following article, BREAKING: Ex-Cop Father and Son Arrested and Charged With Murder of Black Man Jogging In Neighborhood…President Trump Responds [VIDEO], was first published on 100PercentFedUp.com. The Georgia Bureau of Investigations has arrested a father and son duo, 64-year-old ex-cop, Gregory McMichael, and his son, 34-year-old Travis McMichael for the February murder of 24-year-old Ahmaud Arbery, a black man who was jogging through their neighborhood when they jumped in their truck and pursued him. Yashar Ali shared the news of the […] Continue reading: BREAKING: Ex-Cop Father and Son Arrested and Charged With Murder of Black Man Jogging In Neighborhood…President Trump Responds [VIDEO] ... Full Article Featured
ted Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad By 100percentfedup.com Published On :: Sat, 09 May 2020 01:45:58 +0000 The following article, Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad, was first published on 100PercentFedUp.com. Last night, Jimmy Kimmel, host of the low-rated, late-night Jimmy Kimmel Show, shared a deceptively edited video clip of Vice President Pence delivering PPE to a nursing home. Today, liberal activist Matt McDermott tweeted the videotaped segment on VP Pence that was edited to make the vice president look like he was faking a delivery […] Continue reading: Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad ... Full Article Featured Politics
ted Video: Dallas Salon Owner Shelley Luther Gets Surprise Visit from Sen Ted Cruz for a Celebratory Haircut By 100percentfedup.com Published On :: Sat, 09 May 2020 13:19:16 +0000 The following article, Video: Dallas Salon Owner Shelley Luther Gets Surprise Visit from Sen Ted Cruz for a Celebratory Haircut, was first published on 100PercentFedUp.com. When Dallas, Texas salon owner Shelley Luther opened her salon in defiance of the lockdown order in Texas, she was visited numerous times by the local police and then sentenced to 7 days in jail with a fine of $7,000. After public outrage at her punishment, the Texas Supreme Court stepped in to demand her […] Continue reading: Video: Dallas Salon Owner Shelley Luther Gets Surprise Visit from Sen Ted Cruz for a Celebratory Haircut ... Full Article Breaking Featured Government News Politics
ted Traffic stop in Windsor leads to multiple charges and discovery of homemade conducted energy weapon By windsor.ctvnews.ca Published On :: Fri, 8 May 2020 15:00:00 -0400 After being pulled over for what started as a traffic violation, two Windsor men were arrested and face multiple drug, property, and weapon related charges. Full Article
ted OPP release composite drawing of man who allegedly impersonated an officer By windsor.ctvnews.ca Published On :: Fri, 8 May 2020 12:53:00 -0400 Essex County OPP have released a composite drawing of a suspect reported to be impersonating a police officer in Lakeshore. Full Article
ted Overnight snow expected in areas of Southern Manitoba By winnipeg.ctvnews.ca Published On :: Fri, 8 May 2020 17:07:00 -0600 Environment Canada has issued a special weather statement for the southwestern area of Manitoba. Full Article
ted ER stress increases store-operated Ca2+ entry (SOCE) and augments basal insulin secretion in pancreatic beta cells [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-04-24T06:08:45-07:00 Type 2 diabetes mellitus (T2DM) is characterized by impaired glucose-stimulated insulin secretion and increased peripheral insulin resistance. Unremitting endoplasmic reticulum (ER) stress can lead to beta-cell apoptosis and has been linked to type 2 diabetes. Although many studies have attempted to link ER stress and T2DM, the specific effects of ER stress on beta-cell function remain incompletely understood. To determine the interrelationship between ER stress and beta-cell function, here we treated insulin-secreting INS-1(832/13) cells or isolated mouse islets with the ER stress–inducer tunicamycin (TM). TM induced ER stress as expected, as evidenced by activation of the unfolded protein response. Beta cells treated with TM also exhibited concomitant alterations in their electrical activity and cytosolic free Ca2+ oscillations. As ER stress is known to reduce ER Ca2+ levels, we tested the hypothesis that the observed increase in Ca2+ oscillations occurred because of reduced ER Ca2+ levels and, in turn, increased store-operated Ca2+ entry. TM-induced cytosolic Ca2+ and membrane electrical oscillations were acutely inhibited by YM58483, which blocks store-operated Ca2+ channels. Significantly, TM-treated cells secreted increased insulin under conditions normally associated with only minimal release, e.g. 5 mm glucose, and YM58483 blocked this secretion. Taken together, these results support a critical role for ER Ca2+ depletion–activated Ca2+ current in mediating Ca2+-induced insulin secretion in response to ER stress. Full Article
ted Non-photopic and photopic visual cycles differentially regulate immediate, early, and late phases of cone photoreceptor-mediated vision [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 Cone photoreceptors in the retina enable vision over a wide range of light intensities. However, the processes enabling cone vision in bright light (i.e. photopic vision) are not adequately understood. Chromophore regeneration of cone photopigments may require the retinal pigment epithelium (RPE) and/or retinal Müller glia. In the RPE, isomerization of all-trans-retinyl esters to 11-cis-retinol is mediated by the retinoid isomerohydrolase Rpe65. A putative alternative retinoid isomerase, dihydroceramide desaturase-1 (DES1), is expressed in RPE and Müller cells. The retinol-isomerase activities of Rpe65 and Des1 are inhibited by emixustat and fenretinide, respectively. Here, we tested the effects of these visual cycle inhibitors on immediate, early, and late phases of cone photopic vision. In zebrafish larvae raised under cyclic light conditions, fenretinide impaired late cone photopic vision, while the emixustat-treated zebrafish unexpectedly had normal vision. In contrast, emixustat-treated larvae raised under extensive dark-adaptation displayed significantly attenuated immediate photopic vision concomitant with significantly reduced 11-cis-retinaldehyde (11cRAL). Following 30 min of light, early photopic vision was recovered, despite 11cRAL levels remaining significantly reduced. Defects in immediate cone photopic vision were rescued in emixustat- or fenretinide-treated larvae following exogenous 9-cis-retinaldehyde supplementation. Genetic knockout of Des1 (degs1) or retinaldehyde-binding protein 1b (rlbp1b) did not eliminate photopic vision in zebrafish. Our findings define molecular and temporal requirements of the nonphotopic or photopic visual cycles for mediating vision in bright light. Full Article
ted Reactive dicarbonyl compounds cause Calcitonin Gene-Related Peptide release and synergize with inflammatory conditions in mouse skin and peritoneum [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 The plasmas of diabetic or uremic patients and of those receiving peritoneal dialysis treatment have increased levels of the glucose-derived dicarbonyl metabolites like methylglyoxal (MGO), glyoxal (GO), and 3-deoxyglucosone (3-DG). The elevated dicarbonyl levels can contribute to the development of painful neuropathies. Here, we used stimulated immunoreactive Calcitonin Gene–Related Peptide (iCGRP) release as a measure of nociceptor activation, and we found that each dicarbonyl metabolite induces a concentration-, TRPA1-, and Ca2+-dependent iCGRP release. MGO, GO, and 3-DG were about equally potent in the millimolar range. We hypothesized that another dicarbonyl, 3,4-dideoxyglucosone-3-ene (3,4-DGE), which is present in peritoneal dialysis (PD) solutions after heat sterilization, activates nociceptors. We also showed that at body temperatures 3,4-DGE is formed from 3-DG and that concentrations of 3,4-DGE in the micromolar range effectively induced iCGRP release from isolated murine skin. In a novel preparation of the isolated parietal peritoneum PD fluid or 3,4-DGE alone, at concentrations found in PD solutions, stimulated iCGRP release. We also tested whether inflammatory tissue conditions synergize with dicarbonyls to induce iCGRP release from isolated skin. Application of MGO together with bradykinin or prostaglandin E2 resulted in an overadditive effect on iCGRP release, whereas MGO applied at a pH of 5.2 resulted in reduced release, probably due to an MGO-mediated inhibition of transient receptor potential (TRP) V1 receptors. These results indicate that several reactive dicarbonyls activate nociceptors and potentiate inflammatory mediators. Our findings underline the roles of dicarbonyls and TRPA1 receptors in causing pain during diabetes or renal disease. Full Article
ted Webinar: Federalism in a Fragmented State: Rethinking Decentralization in Yemen By feedproxy.google.com Published On :: Tue, 07 Apr 2020 11:40:01 +0000 Research Event 15 April 2020 - 1:00pm to 2:00pm Event participants Osamah Al Rawhani, Deputy Director, Sana’a Center for Strategic StudiesModerator: Nadim Houry, Executive Director, Arab Reform Initiative Yemen suffered from the excessive control of the central government prior to the current conflict. Federalism has been put forward by many Yemeni political parties since the National Dialogue Conference (NDC) as the supposed magic cure for this significant problem. Today, Yemen is more fragmented than ever, its state central institutions have been scattered and lack leadership and the state has lost most of its sovereignty. The prevailing narrative that decentralization through federalism is Yemen’s inevitable path post-conflict often fails to acknowledge that there are prerequisites for effective local governance, beyond political will. In a recent article, Osamah Al Rawhani addressed how the weakness of central state institutions is the key challenge to proceeding with federalism in Yemen and highlighted prerequisites and contextual factors that need to be addressed before reforming the structure of the state. He argued that the viability of decentralization relies on the presence of a functioning, representative central government that is capable of devolving power but also able to keep the state from further fragmentation. In this webinar, part of the Chatham House project on The Future of the State in the Middle East and North Africa, the article’s author will discuss recent developments in Yemen, where shifting frontlines and regional divisions are fragmenting the country in new ways. The speaker will explore alternative approaches to pursue the path of federalism that recognize the current realities and the critical need for strong central institutions. He will also survey the internal and external factors that must be considered to rebuild a stable state in Yemen.You can express your interest in attending by following this link. You will receive a Zoom confirmation email should your registration be successful. Alternatively, you can watch the event live on the MENA Programme Facebook page. Department/project Middle East and North Africa Programme, The Future of the State in the Middle East Reni Zhelyazkova Programme Coordinator, Middle East and North Africa Programme +44 (0)20 7314 3624 Email Full Article
Reni Zhelyazkova Programme Coordinator, Middle East and North Africa Programme +44 (0)20 7314 3624 Email
ted 5-Ethynyl-2'-deoxycytidine and 5-ethynyl-2'-deoxyuridine are differentially incorporated in cells infected with HSV-1, HCMV, and KSHV viruses [Microbiology] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 Nucleoside analogues are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine analogues 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyl-2'-deoxycytidine (EdC). Click chemistry enables conjugation of an azide molecule tagged with a fluorescent dye or biotin to the alkyne of the analog, which can then be used to detect incorporation of EdU and EdC into DNA. The use of EdC is often recommended because of the potential cytotoxicity associated with EdU during longer incubations. Here, by comparing the relative incorporation efficiencies of EdU and EdC during short 30-min pulses, we demonstrate significantly lower incorporation of EdC than of EdU in noninfected human fibroblast cells or in cells infected with either human cytomegalovirus or Kaposi's sarcoma-associated herpesvirus. Interestingly, cells infected with herpes simplex virus type-1 (HSV-1) incorporated EdC and EdU at similar levels during short pulses. Of note, exogenous expression of HSV-1 thymidine kinase increased the incorporation efficiency of EdC. These results highlight the limitations when using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recovery and sensitivity for downstream applications. This is an important discovery that may help to better characterize the biochemical properties of different nucleoside analogues with a given kinase, ultimately leading to significant differences in labeling efficiency of nascent DNA. Full Article
ted The streptococcal multidomain fibrillar adhesin CshA has an elongated polymeric architecture [Microbiology] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 The cell surfaces of many bacteria carry filamentous polypeptides termed adhesins that enable binding to both biotic and abiotic surfaces. Surface adherence is facilitated by the exquisite selectivity of the adhesins for their cognate ligands or receptors and is a key step in niche or host colonization and pathogenicity. Streptococcus gordonii is a primary colonizer of the human oral cavity and an opportunistic pathogen, as well as a leading cause of infective endocarditis in humans. The fibrillar adhesin CshA is an important determinant of S. gordonii adherence, forming peritrichous fibrils on its surface that bind host cells and other microorganisms. CshA possesses a distinctive multidomain architecture comprising an N-terminal target-binding region fused to 17 repeat domains (RDs) that are each ∼100 amino acids long. Here, using structural and biophysical methods, we demonstrate that the intact CshA repeat region (CshA_RD1–17, domains 1–17) forms an extended polymeric monomer in solution. We recombinantly produced a subset of CshA RDs and found that they differ in stability and unfolding behavior. The NMR structure of CshA_RD13 revealed a hitherto unreported all β-fold, flanked by disordered interdomain linkers. These findings, in tandem with complementary hydrodynamic studies of CshA_RD1–17, indicate that this polypeptide possesses a highly unusual dynamic transitory structure characterized by alternating regions of order and disorder. This architecture provides flexibility for the adhesive tip of the CshA fibril to maintain bacterial attachment that withstands shear forces within the human host. It may also help mitigate deleterious folding events between neighboring RDs that share significant structural identity without compromising mechanical stability. Full Article
ted Eliza Manningham-Buller Appointed as Co-President of Chatham House By feedproxy.google.com Published On :: Tue, 21 Jul 2015 13:10:28 +0000 22 July 2015 Manningham-BullerElizahz1.jpg Eliza Manningham-Buller is confirmed as a president of Chatham House replacing Paddy Ashdown who steps down after 10 years.The appointment of Baroness Manningham-Buller as a president of Chatham House was confirmed at the institute’s annual general meeting on Tuesday 21 July. Baroness Manningham-Buller joins Sir John Major and Baroness Scotland of Asthal as a co-president and succeeds Lord Ashdown of Norton-sub-Hamdon who has stepped down from the role after two terms.Eliza Manningham-Buller was director-general of the UK Security Service (MI5) between 2002 and 2007 and became an independent life peer in 2008. She served as Chairman of Imperial College London from 2011 to 2015. She brings to Chatham House an extensive knowledge of and experience in international security as well as a deep interest in medical research and global health, having served as a member of the Board of Governors of the Wellcome Trust since 2008. In October 2015, Baroness Mannigham-Buller will become Chairman of the Trust’s Board of Governors.Paddy Ashdown steps down after serving as a president for 10 years alongside Sir John Major, Patricia Scotland and their predecessors Lord Hurd of Westwell and Lord Robertson of Port Ellen, respectively. Over that period, the institute benefitted enormously from his extensive experience in international politics and conflict resolution, including as High Representative for Bosnia and Herzegovina from 2002 to 2006.Dr Robin Niblett, director of Chatham House, said:'I am delighted to welcome Elizabeth Manningham-Buller as a president of Chatham House. Throughout her career, she has brought deep knowledge, careful analysis and sound judgement to bear upon some of the most difficult dimensions of public policy. The institute will benefit greatly from these qualities as it draws on her engagement with Chatham House over the coming years.I would like to pay tribute to Paddy Ashdown for his long-standing support of Chatham House. His contributions to our substantive debates, both internally and externally, have been invaluable on numerous occasions, and we look forward to his continued involvement with the institute as a member of our Panel of Senior Advisers.'Baroness Manningham-Buller said:“I am delighted to be elected as a Chatham House president at this important time in the institute’s history, as it grapples with a complex and inter-connected agenda of policy challenges. I look forward to working with John Major and Patricia Scotland in supporting Chatham House and its valuable and necessary work. Editor's notes A president’s term at Chatham House is for five years, renewable once. There are no governance responsibilities, which reside solely with the institute’s Council.Chatham House’s three presidents underpin the institute’s independent, non-partisan voice on international affairs. The presidents confirm, through their experiences at the highest levels of government and diplomacy, the connection between Chatham House and policy-makers. Full Article
ted Risk of serious cyber attacks on nuclear facilities underestimated, says new report By feedproxy.google.com Published On :: Fri, 09 Oct 2015 15:22:30 +0000 2 October 2015 20150930CyberSecurity.jpg Workers at the Wolsong nuclear power plant participate in an anti-cyber attack exercise, Gyeongju, South Korea. Photo: Getty Images. The risk of a serious cyber attack on civil nuclear infrastructure is growing, as facilities become ever more reliant on digital systems and make increasing use of commercial ‘off-the-shelf’ software, according to a major new report from Chatham House.Cyber Security at Civil Nuclear Facilities: Understanding the Risks is the result of an 18-month study that draws on in-depth interviews with 30 leading industry practitioners based in more than eight countries. It found that the trend to digitization, when combined with a lack of executive-level awareness of the risks involved, means that nuclear plant personnel may not realize the full extent of their cyber vulnerability and are thus inadequately prepared to deal with potential attacks. Specific findings include: The conventional belief that all nuclear facilities are ‘air gapped’ (isolated from the public internet) is a myth. The commercial benefits of internet connectivity mean that a number of nuclear facilities now have VPN connections installed, which facility operators are sometimes unaware of.Search engines can readily identify critical infrastructure components with such connections.Even where facilities are air gapped, this safeguard can be breached with nothing more than a flash drive.Supply chain vulnerabilities mean that equipment used at a nuclear facility risks compromise at any stage.A lack of training, combined with communication breakdowns between engineers and security personnel, means that nuclear plant personnel often lack an understanding of key cyber security procedures.Reactive rather than proactive approaches to cyber security contribute to the possibility that a nuclear facility might not know of a cyber attack until it is already substantially under way.In the light of these risks, the report outlines a blend of policy and technical measures that will be required to counter the threats and meet the challenges. Recommendations include:Developing guidelines to measure cyber security risk in the nuclear industry, including an integrated risk assessment that takes both security and safety measures into account.Engaging in robust dialogue with engineers and contractors to raise awareness of the cyber security risk, including the dangers of setting up unauthorized internet connections.Implementing rules, where not already in place, to promote good IT hygiene in nuclear facilities (for example to forbid the use of personal devices) and enforcing rules where they do exist.Improving disclosure by encouraging anonymous information sharing and the establishment of industrial CERTs (Computer Emergency Response Team).Encouraging universal adoption of regulatory standards. Editor's notes Cyber Security at Civil Nuclear Facilities: Understanding the Risks, written by Caroline Baylon with Roger Brunt and David Livingstone, is embargoed until Monday 5 October 2015.Caroline Baylon is the research associate in science, technology and cyber security at Chatham House. Roger Brunt was appointed the UK government's regulator for security in the civil nuclear industry as the director of the Office for Civil Nuclear Security after retiring from the British Army in 2004. David Livingstone is an associate fellow at Chatham House, where he has participated in a broad range of projects on national-level risk management, cyber security, counterterrorism, serious organized crime, nuclear security and space security.Fore more information, or to request an interview with the authors, contact the press office Contacts Press Office +44 (0)20 7957 5739 Email Full Article
ted The resource curse has not been lifted By feedproxy.google.com Published On :: Fri, 09 Oct 2015 15:40:02 +0000 5 August 2015 20150804ResourceCurse2.jpg Hoping to make a little money from Sudan's ocean of black gold, a woman sells tea to roughnecks at an oil rig near Bentiu, Sudan. Photo by Getty Images. During a decade-long commodities boom, new or emerging producers of oil, gas or mineral resources registered some of the fastest rates of economic growth in the world. Development banks, governments giving foreign aid, extractives companies and major consultancies broadly agreed that ‘extractives-led growth’ is a viable path to socio-economic development for poor countries. Following over a year of decline in global commodities prices and as efforts to tackle climate change mount, a new paper re-examines the 'curse of natural resources'. It finds that a policy of extractives-led growth entails serious risks. As governments of countries as diverse as Afghanistan, Mauritania, Somalia, Liberia and Cuba prepare to follow an extractives-led growth path, both the advice being handed to them and the growth model itself require a fundamental rethink.The Resource Curse Revisited argues that:The steep decline in the oil price in the second half of 2014 demolished the main assumption of the extractives-led growth agenda. The assumption that prices of raw materials would continue to increase as global demand grew and well-established sources were exhausted has actually led several low- to middle-income producers such as Ghana into unmanageable debt. At the very least, the current price context puts new producers at a serious disadvantage, as the focus on cost-cutting has made investors reluctant to accept the risks of developing projects in countries with little infrastructure or capacity to support them.Good governance initiatives are not the antidote to the resource curse. There has often been a mismatch in terms of policy advice given (for example on transparency and revenue management) and the capacity of a country to implement it. Furthermore, basing economic growth on the extraction of below-ground resources will create strong pressures towards poor governance. In the absence of strong institutions, this path leads to the enrichment of minority elite groups, whose interest in capturing rents is likely to become a barrier to improving governance.Both governments with extractives potential and those advising them give too little consideration to the size and nature of the resource base. If extractives-led growth is to be sustained, resource extraction must persist long enough for new economic sectors to emerge and generate revenues that can support government spending and import needs as income from extractives declines.The extractives-led growth model, in its current form, is at odds with green growth strategies. The advice from international agencies and initiatives to countries with extractive resources offers no suggestions on how governments should manage the risk of stranded assets or how they can reconcile extractives-led growth with national sustainable-development goals.The report concludes that the extractives-led growth agenda has tended to reinforce domestic, government and investor pressures to ‘develop fast’. However, this can threaten long-term opportunities for robust economic diversification. In many cases, there is a strong case for slowing development of extractives projects to allow time to develop the capacity of the government and the private sector to maximize the linkages with the rest of the economy.Avoiding the resource curse needs not only good governance but also an economic policy that provides for the transition of an economy over time in accordance with its competitive advantages. This report recommends that countries considering extractives development, and their would-be advisors, take into account a wider set of issues at the outset including the likely value of the asset to the economy over time, the options for slow or indeed no development of extractives, and how the rest of the economy would lessen reliance on support from the extractives sector over time. Editor's notes Read the report The Resource Curse Revisited from the Energy, Environment and Resources Department, Chatham House.For all enquiries, please contact the press office Contacts Press Office +44 (0)20 7957 5739 Email Full Article
ted Jim O’Neill elected new chair of Chatham House By feedproxy.google.com Published On :: Wed, 18 Apr 2018 13:39:42 +0000 19 April 2018 JimONeill1.jpg Photo: Jim O'Neill. In the post of chair, Jim O’Neill will lead the council in overseeing the operations and performance of the institute as well as contribute his substantial experience to support its mission to help build a sustainably secure, prosperous and just world. As Chatham House approaches the centenary of its founding in 2020, he will also work closely with council members, the institute’s supporters and its director, Robin Niblett, to ensure that the institute continues to be at the forefront of debate, analysis and new ideas on the most critical issues in international affairs.Jim O’Neill will take over from Stuart Popham QC, who has served six years in the post, after the institute’s AGM in July 2018. The selection was undertaken by a search committee led by Sir Simon Fraser, vice chair of Council and chair of the nominations committee, working alongside MWM Consulting.Stuart Popham said: ‘I am delighted that members of the Chatham House Council have unanimously elected Jim O'Neill to be my successor as chair. Jim brings a wealth of relevant experience to the role, which will stand him in good stead in leading the council. As Chatham House consolidates its position after several years of growth and moves towards the historic celebration of its centenary, he is well-placed to advise the executive team on how best to leverage its strengths and build for the future.’Lord O’Neill said: ‘I welcome this opportunity to lead the board of an institution that I greatly respect and that I believe will play a highly important role in the future. Uniquely, Chatham House combines the capacity to convene leading thinkers and practitioners on international affairs alongside deep knowledge on how to confront some of the most intractable global challenges of our time. I look forward to building on Stuart's legacy and to ensuring Chatham House addresses the risks and opportunities of the future with the same passion as it has in the past.’Robin Niblett said: ‘Jim O'Neill is one of the world’s the most perceptive analysts and thinkers about the global economy. His appointment as chair-elect of Chatham House coincides with a period when many of the structures and principles that have supported global growth and buttressed peace are under threat. We are fortunate that an individual of Jim's calibre will help guide the institute during this period of profound change and enable us to engage even more intensively with our many constituencies around the world.I am also enormously grateful for Stuart Popham's support these past six years, during which he has led the institute through a period of unprecedented expansion and a further strengthening of its international reputation.’Stuart Popham QC steps down after two three-year terms as the institute’s chair during which time Chatham House has broadened its areas of research, including the establishment of the Hoffmann Centre on Sustainable Resource Economy, launched the Queen Elizabeth Academy II Academy for Leadership in International Affairs, and created modern new meeting facilities in the Stavros Niarchos Foundation Floor of the adjoining Ames House.The post of chair is elected by members of council who, in turn, are drawn from and elected by the institute’s individual and nominated members.Jim O’Neill’s previous roles include as joint head of research at Goldman Sachs (1995–2000), its chief economist (2001–10) and chairman of its asset management division (2010–13); commercial secretary to the Treasury (2015-16); chair of the City Growth Commission (2014); and chair of the Review on Anti-Microbial Resistance (2014-16). He was created a life peer in 2015, and serves as a crossbench member of the House of Lords. He is also an honorary professor of economics, University of Manchester, and holds honorary degrees from the University of Sheffield, University of London and from City University London. He received his PhD from the University of Surrey. Full Article
ted Creon Butler appointed to lead Global Economy and Finance Programme By feedproxy.google.com Published On :: Tue, 22 Oct 2019 10:22:32 +0000 22 October 2019 Creon Butler has been appointed to lead the Global Economy and Finance programme at Chatham House, joining the institute at the beginning of December. He will also form part of the institute’s senior leadership team. Creon will join Chatham House from the Cabinet Office where he served as director for international economic affairs in the National Security Secretariat and G7/G20 ‘sous sherpa’, advising on global policy issues such as climate change, natural resource security, global health threats and the future of the international economic architecture.Creon first joined the Cabinet Office in 2013 as director in the European and Global Issues Secretariat, advising prime minister David Cameron on international economic and financial issues, ranging from country-specific developments in China and Germany to global challenges such as antimicrobial resistance and anticorruption. He designed and organized the UK’s global Anti-Corruption Summit in May 2016. Earlier in his career, he served in the Bank of England, HM Treasury and in the Foreign and Commonwealth Office, where he was director for economic policy and chief economic adviser. He was also deputy high commissioner in New Delhi from 2006 to 2009.Robin Niblett, director of Chatham House, said: 'We are delighted that Creon Butler will join Chatham House at such an important moment, when geoeconomic competition and technological disruption are changing the structure of the global economy, and as governments and societies across the world must develop more sustainable pathways to economic growth. Creon brings precisely the right combination of knowledge and experience to enable Chatham House to conceive inclusive solutions for the future.'Creon Butler said: “Chatham House’s high quality, independent and focused policy research has never been more important in helping policy makers to chart the best path given today’s extraordinary economic and political uncertainties. I am very pleased to have the opportunity to lead the institute’s Global Economy and Finance programme at this critical time.' Full Article
ted Close but Distracted: The Future of the UK-US Partnership By feedproxy.google.com Published On :: Thu, 16 Oct 2014 14:00:01 +0000 Invitation Only Research Event 16 October 2014 - 9:00am to 10:30am Chatham House, London Meeting Summarypdf | 145.17 KB Transcriptpdf | 45.62 KB In an open podcast recorded following the event, Robin Niblett discussed the state of the transatlantic relationship with International New York Times London Bureau Chief Steven Erlanger, former UK defence secretary Liam Fox and former Foreign Affairs Committee chair Mike Gapes.The roundtable was held under the Chatham House Rule. While revelations about the activities of the NSA and GCHQ confirmed the depth of cooperation between the US and the UK over intelligence, the debate over the UK’s membership of the EU and difficulties in coordinating a response to the Syria crisis have raised tensions between policy-makers in Washington and London. With the two countries now facing an array of shared international challenges – not least in Ukraine, Afghanistan and Iraq – this roundtable will examine the current state of the transatlantic relationship. Event attributes Chatham House Rule Full Article
ted Allies Beware: Americans Support a More Limited Role for the US By feedproxy.google.com Published On :: Wed, 11 May 2016 12:45:03 +0000 12 May 2016 Bruce Stokes Associate Fellow, US and the Americas Programme (based in the US) @bruceestokes Xenia Wickett Former Head, US and the Americas Programme; Former Dean, The Queen Elizabeth II Academy for Leadership in International Affairs @xeniawickett LinkedIn While not as isolationist or unilateralist as some campaign rhetoric might suggest, new polling shows the American public broadly supports less engagement with the rest of the world. 2016-05-11-US-Philippines.jpg US military personnel take part in joint military exercises with the Philippines, Australia and Japan in Crow Valley on 14 April 2016. Photo by Getty Images. While the American election cycle could be perceived as an occasionally amusing distraction, the rise of new factions and sentiments among the electorate will have an impact not just in America’s domestic politics but also with respect to its role in the world. Recent polling by the Pew Research Center shows clearly the desire among many Americans for a different international engagement for the US, one that could have significant implications for America’s allies.PollingTo date, the campaign rhetoric of both the Republican and Democratic contenders for the White House has raised questions about America’s continuing global commitment.But what does the public think? The Pew Research Center has recently released its periodic survey of how Americans view America’s place in the world. The results suggest that stereotypes of Americans’ isolationism or protectionism do not capture the nuance in public sentiment. Wariness of international engagement coexists with assertiveness on some issues and a belief that the US is a force for good in the world. And these views often divide along partisan lines and between generations.A majority of Americans (57%) think the U.S. should deal with its own problems and let other countries deal with theirs as best they can, a sentiment that has increased from 46% in 2010. Moreover, roughly two-thirds say ‘we should not think so much in international terms but concentrate more on our own national problems’. In part this may be the case because a plurality of Americans thinks the United States does too much (41% too much, 27% too little) in helping solve world problems.The fact that six-in-ten Americans believe that problems in the world would be even worse without US involvement will not reassure many non-Americans who care deeply about how and where the US is engaged, not just that it is.American isolationism is a partisan affair. Republicans (62%) are far more likely than Democrats (47%) to voice the view that the US should deal with its own problems. Notably, about two-thirds of registered Republicans or Republican-leaning independents who favour Donald Trump (68%) express such isolationist sentiments, as do more than half of the Democratic backers of Sen. Bernie Sanders (54%). At the same time, over half of registered Democrats and Democratic-leaning independents who favour Hillary Clinton (52%) believe the US should help other countries deal with their problems.The US has often been seen by its allies as acting in a unilateralist fashion, but the polling is more nuanced than this. About half (51%) believe that the US should take into account the views of its major allies when deciding its foreign policies. However, of more concern for America’s allies (and potentially adversaries), roughly four-in-ten (42%) believe Washington should go it alone in international matters.Despite Trump’s criticisms, 53% of the American public holds a positive view of NATO and 77% voice the view that US membership in the security alliance has been a good thing for the United States.In addition to some wariness with regards to engaging internationally in security arenas, Americans are also generally wary of global economic engagement – protectionist sentiment is rising. Only 44% believe such US involvement is a good thing; more (49%) say such engagement is bad, lowering wages and costing jobs. These views also differ along partisan lines with more on the right than left thinking US involvement in the world economy has been a bad thing. Trump supporters are even more against economic globalization.Rising scepticismThe results do not show an unambiguous move towards more American isolationism or unilateralism. But the trends broadly show that the American public is moving towards, as President Obama put it in 2012, a focus on ‘nation building at home’. It should come as no surprise – President Obama has presided over a period in which the US has increasingly limited its extraterritorial ambitions to those that more directly affect its vital national interests. It is therefore worth noting that this more limited engagement is one that is, broadly, supported by the public on both sides of the aisle.There are, as have been noted, partisan differences. But the next president will govern over all Americans not just those from their own party. They will have to function in the context of an American public, the majority of whom wants the US to deal with its own problems, letting other countries manage as best they can. While who becomes president clearly matters – they have the capacity to lead their populations in certain directions - the rising scepticism among many Americans for investing in the globalized world will resonate regardless of who takes office.The implications for America’s allies are significant. For those issues that are not directly of concern to the United States, longstanding partners are going to have to find ways to manage on their own or with less American support. And even on those issues that are of direct national interest, they might find the US less sensitive to their interests and concerns. This could hold true not just in the realm of security but on economic issues also – if America becomes more protectionist, others will surely follow. Many of America’s allies have perceived the United States to be an unreliable ally in recent years. Asian partners, such as Japan and South Korea, have started to take more responsibility for their own security and are working to build additional partnerships with neighbours. European allies have been, at times, disappointed by what they perceive to be a less engaged America, such as in Libya and Syria. If the next president follows the public majority, these allies will need to get used to this new role for the United States.This article has been published jointly with Real Clear World.To comment on this article, please contact Chatham House Feedback Full Article