In the late 19th century, city officials turned the final resting place for 10,000 souls into what's now Greenwich Village’s James J. Walker Park

Iconic images of Dr. King from the Smithsonian collection

The surge of hungry four-legged visitors to Belushya Guba is part of a larger trend

A new book charts the history of pet cemeteries and honors the universal experience of grieving an animal companion

These images from the Smithsonian Magazine Photo Contest show how communities in Mexico and beyond mark Día de los Muertos.

Shallaway Youth Choir is bringing a program of music to the Basilica in downtown St. John’s on Remembrance Day. Artistic director Kellie Walsh says the choir will sing about such topics as the Unknown Soldier and schoolchildren who care for Newfoundland graves in London. Where Once They Stood will be performed at 2 p.m. and 7 p.m. on Monday.

Large crowds came together at city hall in Corner Brook to lay wreaths and honour veterans on Monday. The ceremony portion was completed with a playing of the Last Post.

Hundreds congregated in downtown St. John's Monday morning to pay tribute to Newfoundland and Labrador's fallen soldiers, and honoured the dead for the first time at the newly refurbished National War Memorial and tomb of the Unknown Solider. 

The L'nu Sipuk Kina'muokuom school observed Indigenous Veterans Day with an event on Friday. Students, faculty and staff got together to honour Mi’kmaw military veterans and RCMP members.

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

The only daily TV news package to focus on Nova Scotians and their stories

About a thousand people stood solemnly in the rain at a cenotaph in downtown Halifax on Monday morning to pay tribute to veterans on Remembrance Day.

The only daily TV news package to focus on Nova Scotians and their stories

The Montreal English Theatre Awards ceremony is being held this evening at Le Gesù theatre. For the community, it's a time to celebrate their achievements and resilience coming out of the pandemic, together.

The Société de transport de Montréal (STM) has established a non-urgent text line for transit users to signal security issues in the Metro network.

Facing soaring food prices and an escalating housing crisis, Quebec student associations are urgently calling on the provincial government to fund internships required for post-secondary training.

Mosquito-borne disease is a major global public health issue. One path toward the development of evidence-based strategies to limit mosquito biting is the study of the mosquito nervous system—in particular, the sensory systems that drive biting behavior. The central nervous system of insects consists of the brain and the ventral nerve cord. Here, we describe a protocol for dissecting, immunofluorescent labeling, and imaging both of these structures in the mosquito. This protocol was optimized for Aedes aegypti and works well on Anopheles gambiae tissue. It has not been tested in other mosquito species, but we anticipate that it would work on a range of mosquitoes, and, if not, our protocol will provide a starting point from which to optimize. Notably, a limited number of antibodies cross-react with Ae. aegypti proteins. This protocol is intended for use with validated antibodies and can also be used to test new antibodies as they are generated. It has been successfully used to visualize protein tags, such as green fluorescent protein, that have been introduced into the mosquito to amplify or detect their presence.

The technique of visualizing axon pathways in the embryonic ventral nerve cord using antibody labeling has been fundamental to our understanding of the genetic and developmental mechanisms underlying nervous system wiring in Drosophila. High-resolution microscopic examination of the ventral nerve cord remains an essential component of many experiments in Drosophila developmental neuroscience. Although it is possible to examine the ventral nerve cord in intact whole-mount embryos, to collect the highest-quality images it is often useful to isolate the nervous system away from the other embryonic tissues through embryo dissection. This protocol describes methods for dissecting ventral nerve cords from Drosophila embryos that have been fixed and stained via immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry. The process of making fine dissection needles for this purpose from electrolytically sharpened tungsten wire is also described. Dissected and mounted ventral nerve cords can be examined and imaged using a variety of microscopy techniques including differential interference contrast (DIC) optics, epifluorescence, or confocal microscopy.

The Drosophila embryonic central nervous system has been used for decades as a model for understanding the genetic regulation of axon guidance and other aspects of neural development. Foundational studies using antibody staining to examine the embryonic ventral nerve cord in wild-type and mutant animals led to the discovery of evolutionarily conserved genes that regulate fundamental aspects of axon guidance, including midline crossing of axons. The development of the regular, segmentally repeating structure of axon pathways in the ventral nerve cord can illustrate basic principles of axon guidance to beginning students and can also be used by expert researchers to characterize new mutants, detect genetic interactions between known genes, and precisely quantify variations in gene function in engineered mutant lines. Here, we describe a protocol for collecting and fixing Drosophila embryos and visualizing axon pathways in the embryonic ventral nerve cord using immunofluorescence or immunohistochemical staining methods. As embryogenesis in Drosophila takes ~24 h to complete, a 1-d collection yields embryos representing all stages of development from newly fertilized through ready-to-hatch larvae, allowing investigation of multiple developmental events within a single batch of collected embryos. The methods described in this protocol should be accessible to introductory laboratory courses as well as seasoned investigators in established research laboratories.

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

As people across the country mourn the Honourable Justice Murray Sinclair, some in the Northwest Territories justice system are reflecting on his contributions to the country — one of those being Gladue principles. 

A group of volunteers have been providing emergency services to West Dawson residents during the periods of freeze up and break up, when there is no reliable access across the Yukon River to town.

Historians say that Sarah Emma Edmonds exaggerated many aspects of her wartime experiences. Still, she bravely served in the Union Army, becoming one of hundreds of women who fought in the conflict in secret

In the early 1990s, historians and the public alike questioned how Disney's America would accurately and sensitively document the nation's thorny past

With flinty perseverance and a golden touch, Belinda Mulrooney earned an unlikely fortune in the frozen north and reshaped the Canadian frontier

The 1898 Wilmington massacre left dozens of Black North Carolinians dead. Conspirators also forced the city's multiracial government to resign at gunpoint

Families of Norton Elementary School won’t be seeing their kids return anytime soon. Repairs are going to take longer than expected and students are expected to be back in the school by the start of the next school year.

Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

With Remembrance Day and Indigenous Veterans Day coming up, many people start to reflect on their family's wartime stories by looking through mementos saved by former generations. Ron Kanutski of Thunder Bay, Ont., joins the CBC's Mary-Jean Cormier to talk about his family's military history.

Even as they fundraise for other community organizations, some legions across Canada are having trouble keeping their own lights on amid rising inflation and maintenance costs.

Ginoogaming First Nation has declared a state of emergency after an engineering inspection showed the bridge leading to the community should be closed to traffic.

Since 1970, Lakehead Public Schools has operated Kingfisher Lake Outdoor Education Centre just outside of Thunder Bay, Ont. The CBC's Matt Fratpietro spent a morning with students from École Elsie MacGill Public School to learn more about the forests that surround the city.

In honour of Remembrance Day, Students from St. Paul School visited St Andrew's Catholic Cemetery to clean headstones of veterans, a partnership with the No Stone Left Behind project.

CBC's Superior Morning has been gathering wartime stories from listeners leading up to Remembrance Day based on mementos they have kept for generations. Here's some of the stories residents in northwestern Ontario have shared.

As a troubled local gym goes dark, some members and the landlord say they haven’t heard from the owner about the future of the business in Thunder Bay, Ont.

Wildlife photographer Jason Leo Bantle recounts the moment he came across Banff’s most famous grizzly ‘The Boss,’ during the bear’s surprise visit to Canmore, Alta.

Para Roberto Ramírez de Costa Rica, servir a Dios en misiones ha sido un paso de fe tras otro. Perdió su trabajo, su estabilidad financiera e incluso a su prometida, pero ganó mucho más.

Jeff Monague has worked to incorporate Indigenous elements into Base Borden's Remembrance Day ceremonies — an acknowledgement he never had as a soldier.

A museum in Fort McMurray is helping visitors gain a deeper understanding of Canada’s role in previous wars. The Wood Buffalo Military Museum Centre is offering a chance to get up close to military artifacts. Young visitors say seeing the remnants of conflict gives them a new understanding of Remembrance Day's meaning.

Trevor Lewis was a corporal in the Canadian Armed Forces who tried to bury the memories from his deployment in Afghanistan. They all came back in 2021 after the fall of Kabul. This story is how he made peace with his time in that country.

Former Ohio state Senator Nina Turner discusses the 2024 elections.

Headlines for October 30, 2024; Report from Pennsylvania: Marc Lamont Hill on Harris’s Closing Speech & Dangers of a Trump Victory; Imara Jones: Transphobia Is “Key Pillar” of Trump’s Push for a “Patriarchal Fascist Regime”

Headlines for October 31, 2024; “Genocide as Colonial Erasure”: U.N. Expert Francesca Albanese on Israel’s “Intent to Destroy” Gaza; Bishop William Barber Endorses Harris, Says Faith Leaders Must Oppose Trump’s Hate; “The Racism of MAGA Is as American as Apple Pie”: Nina Turner on Trump & 2024 Election

Headlines for November 01, 2024; Will Abortion Rights Decide 2024 Election? Amy Littlefield on Trump’s Misogyny & 10 Ballot Measures; Report from Wisconsin: John Nichols on Harris’s Madison Roots & Key Senate/House Races Nationwide; “Little Secret”? Elie Mystal on Trump’s Likely Plan to Steal Election with GOP House Speaker Johnson

Headlines for November 04, 2024; “You’re Being Lied To”: Pennsylvania County Elections Chair Debunks Claims of Voter Fraud; Former FEC Counsel Speaks Out on Big Money, Citizens United & Elon Musk’s Illegal Moves to Help Trump; Save the Children in Gaza: Israel Bombs Polio Vax Site, Bans UNRWA in Attacks on Humanitarian Aid; Dearborn Mayor Abdullah Hammoud on Refusing Meeting with Trump, Not Endorsing Harris

Headlines for November 05, 2024; Juan González: Sitting Out This Election Would Be a Mistake, Just as It Was in 1968; 2020 Redux? Army of MAGA Election Officials Prepare to Challenge Results If Trump Loses; Trump Tried to Steal the Vote in Georgia in 2020. Now Election Deniers Run Georgia’s Election System; Ari Berman on Racist Roots of Electoral College & How Ballot Measures Can Help Preserve Democracy; “The Misinformation Web”: Maria Hinojosa on the Pro-Trump Propaganda Targeting Latinos in 2024

Headlines for November 06, 2024; “The Confederacy Won”: Why Donald Trump’s Reelection Is a Win for White Supremacy, Xenophobia & Hate; “This Is a Collapse of the Democratic Party”: Ralph Nader on Roots of Trump’s Win Over Harris; “A Devastating Result”: John Nichols on GOP Taking White House and the Senate; Linda Sarsour: Harris’s Embrace of Pro-Israel Policies at Odds with Democratic Base; Keeanga-Yamahtta Taylor: Democrats Demobilized Their Base. A Movement Is Now Needed to Oppose Trump; Rami Khouri: U.S. Voters Are Sick of Foreign Wars. Can Trump Strike a Grand Bargain in Middle East?; 7 States Vote to Protect Abortion Rights in Busy Year for Ballot Initiatives

Headlines for November 07, 2024; Democrats Abandoned the Working Class: Robin D.G. Kelley on Trump’s Win & Need for Class Solidarity; Fatima Bhutto: Kamala Harris’s Support for Israel’s Genocide in Gaza Is a Betrayal of True Feminism; “Communities Were Destroyed”: Mass Deportations of 1930s & ’50s Show Harm of Trump Plan, If Implemented