Team Homan's decision to part ways with Lisa Weagle dramatically changed the look of one of the country's top rinks and put the all-star lead at the top of curling's free agent list.

The men's curling world championship in Glasgow was cancelled Saturday morning to prevent the spread of COVID-19. Brad Gushue and his rink from Newfoundland and Labrador was going to represent Canada after winning the Brier.

The University of Alberta Pandas defeated the UNB Reds 10-2 in the U Sports women's curling final, while the Laurier Golden Hawks topped the Dalhousie Tigers 8-5 for the men's curling title in Portage la Prairie, Manitoba on Sunday.

The Kevin Koe curling team has split with second Colton Flasch after being eliminated from the recent men's national championship in Kingston, Ont., in a tiebreaker game. Flasch joined the foursome in 2018.

In the span of a few days, Canada's curling landscape has shifted dramatically. The country's past women's and men's Olympic teams and last year's women's world champion team have all split.

John Morris will play second for the Kevin Koe's team for at least the next 18 months heading into the Olympic trials in 2021.

Let go last week after a decade-long run with skip Rachel Homan's Ottawa-based rink, lead Lisa Weagle has joined Team Jennifer Jones, which will operate with five members.

The bonspiel took place in Edmonton March 11-14, starting the same day COVID-19 was declared a global pandemic.

When the World Curling Federation cancelled the women's, men's and mixed doubles championships set for March and April, it said it would be discussing potential options including rescheduling the events — perhaps playing them in the fall at one venue. CBC Sports has learned that will no longer be the case.

The World Curling Federation announced Thursday it is proposing to the International Olympic Committee that the 2021 world curling championships will determine which countries will book their tickets to Beijing 2022.

Colleen Jones, Sidney Crosby among many preeminent local sports figures reacting in the aftermath of Sunday's killing rampage.

The time was right for Lisa Weagle to join forces with Olympic champion Jennifer Jones. Jones told CBC Sports' Anastasia Bucsis that the latest addition to her five-person rink "was like the clouds parted and the sun came out."

He’s a nominee in perpetual waiting.

I just hate it when my fellow Italian-Americans squabble.

He’s not rising to the challenge of the coronavirus pandemic. He’s shriveling into nothingness.

They’re pandemic partners, and that’s double trouble.

Just in time for Easter, the story of a blind state leader who is giving up his office to join the Jesuits.

The notion that he is bound for four more years is pure superstition.

Is the Republican indulgence of the president bottomless?

Laurie Garrett, the prophet of this pandemic, expects years of death and “collective rage.”

Join our Facebook live on Thursday at noon with Dr. Roselyn Wilson a psychiatrist at St. Joseph’s Healthcare Hamilton’s Youth Wellness Centre. She'll talk with us about how COVID-19 is heightening anxiety in some young people. We'll talk about how to help the young people in your life through the pandemic.

Flattening the curve has been so successful and two areas around Hamilton — Brant and Haldimand-Norfolk — haven't had a new case of COVID-19 in three days, and Burlington has only had two.

The province says no one has to tell others if they get COVID-19. The same goes for businesses or landlords, should employees or tenants get sick. But should you tell?

Government restrictions designed to limit the spread of COVID-19 have virtually crippled Ontario's wine-making industry, as retail and wholesale revenues dry up but the costs of producing wine remains constant.

Fears that COVID-19 would spread at the Hamilton-Wentworth Detention Centre appear not to have materialized — at least not yet.

Two additional men have been charged with first-degree murder in the death of a 17-year-old Brampton boy shot and killed on New Year's Eve.

Curbside pickups offer a bit of hope after months of being shuttered by COVID-19, but while retailers are happy to start getting back to business some are raising questions of fairness and access to opportunity.

A Toronto law firm has filed to launch a class action lawsuit against a Niagara Falls long-term care home where 18 residents have died from COVID-19, saying the home had a pub night in the midst of the pandemic.

The COVID-19 pandemic may be keeping families physically distant, but that doesn't mean you can't show someone you care.

Flurries or rain showers are in the forecast for Hamilton on Friday, Environment Canada says.

Sofina Foods plants in Burlington and Mississauga have each had an employee test positive.

A search warrant executed by Hamilton police vice and drugs officers at an address in the city’s east end has resulted in the arrest of a couple and multiple charges, police said in a Friday release.

Dr. Roselyn Wilson, a psychiatrist at St. Joseph’s Healthcare Hamilton’s Youth Wellness Centre, offers some tips to help young people cope with the stress and anxiety they face while on lockdown under COVID-19.

Hamilton Conservation Authority is re-opening the Hamilton-to-Branford Rail Trail, while the city announced the reopening of the Hamilton Waterfront Trail, between Confederation Park and the Burlington Lift Bridge.

McMaster University is planning for fewer international students, more deferred acceptance offers and online teaching for the thousands who are attending as the start of a new school year looms in the near future and in the middle of the COVID-19 pandemic.

The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.

This introduction describes the features and uses of ethidium bromide, methylene blue, and SYBR dyes for staining nucleic acids.

Quantifying RNA is an important and necessary step before most RNA analysis techniques. Methods for quantifying RNA can be classified into two categories: ultraviolet (UV) spectrophotometric methods, which are based on the absorption spectra of the purine and pyrimidine bases; and fluorescent dye-based methods, which measure the fluorescence intensity of dyes that selectively fluoresce when bound to nucleic acids. If the RNA sample is pure (i.e., without significant amounts of contaminants such as proteins, phenol, agarose, or other nucleic acids), UV spectrophotometric measurement of the amount of UV irradiation absorbed by the bases is simple and accurate. However, if the sample contains significant quantities of impurities or if the concentration of RNA is very low, it is better to use fluorescent dye-based methods. An overview of spectrophotometric and fluorescent dye-based RNA quantification methods is given here, as are several options for storing purified RNA preparations. Proper storage of RNA samples is important; it can help minimize RNase contamination and consequent sample degradation.

Determining the concentration of protein samples generally is accomplished either by measuring the UV absorbance at 280 nm or by reacting the protein quantitatively with dyes and/or metal ions (Bradford, Lowry, or BCA assays). For purified proteins, UV absorbance remains the most popular method because it is fast, convenient, and reproducible; it does not consume the protein; and it requires no additional reagents, standards, or incubations. No method of protein concentration determination is perfect because each is subject to a different set of constraints such as interference of buffer components and contaminating proteins in direct UV determination (A₂₈₀) or reactivity of individual proteins and buffer components with the detecting reagents in colorimetric assays. In cases in which protein concentration is critical (e.g., determination of catalytic rate constants for an enzyme), it may be advisable to compare the results of several assays.

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