On Day 3 of TeenStreet there were separate meetings for girls and boys in the Main Hall. The theme of the day was 'influence', and the key verse of the day is found in Proverbs 4:23.

We are meant to get involved in ministry because people need to know about His love. From a duckling to a garbage-can drum, the speakers had powerful examples of the 'why' and 'how' of this call.

Este año en el mes en que se llevó a cabo el mantenimiento anual de Logos Hope en Uruguay, la tripulante Cecilia* de Argentina se unió a un pequeño equipo que sirve en Asia Central. Mientras estuvo allí, pudo asistir a dos iglesias subterráneas que desbordaban de esperanza y fe.

Mar del Plata, Argentina :: Crewmembers visit a Christian rehabilitation centre and remind women of their value in God's eyes.

Mar del Plata, Argentinen :: Schiffsmitarbeiter besuchen ein christliches Suchtrehabilitationszentrum und erinnern die Frauen dort daran, dass sie in Gottes Augen wertvoll sind

Para Roberto Ramírez de Costa Rica, servir a Dios en misiones ha sido un paso de fe tras otro. Perdió su trabajo, su estabilidad financiera e incluso a su prometida, pero ganó mucho más.

Serving in West and Central Asia for many years, Paul and Soonok have one lasting dream: to see a church planting movement there.

Durante un viaje misionero de un mes, Eduardo aprendió a escuchar y reconocer la voz de Dios, tanto personalmente como ministrando a otros.

“Listening to their testimonies, I’ve begun to envision their stories as a collection of clues, a series of scenes revealing the fingerprints of something—or Someone—beyond our deepest imagination,” says Chris. “The people I talked with hail from a variety of backgrounds—atheist German to Cambodian Buddhist—but the tapestries of their lives reveal the same beautiful threads, pointing unmistakably to a Designer.“

Tinashe disciples and shows Zimbabweans that they, too, are called to serve; everyone can share their own stories with others to show the love of Christ. 

Los obreros de OM en la Península Arábiga usan sus empleos de forma intencional como una fuente oportunidades para desarrollar relaciones y compartir la verdad con sus compañeros de trabajo en las naciones menos alcanzadas.

Mar del Plata, Argentina :: A church congregation learns about nations without access to the gospel.

"It is unfortunate that it has come to pass that we have to collectively and overtly publicly declare that we are unified," says Alberta Medical Association president Christine Molnar.

When Elizabeth May, parliamentary leader of the federal Green Party, proclaimed on Tuesday that "oil is dead," she was correct in a philosophical sense. But not in a practical, real world sense.

People and businesses are rallying to support the Muslim community in Fort McMurray recover after widespread flooding that damaged many neighbourhoods.

Take a listen to Episode 9 of CBC Edmonton's new podcast, The Loop.

The vessel, formerly known as the Edmonton Queen, got skewered by its own moorings last month as the icy North Saskatchewan River began to thaw.

Much like a prehistoric pest trapped in amber, our summer plans remain in suspended animation.

Residents are cleaning up damage to hundreds of homes and businesses caused by floods in the northern Alberta hamlet of Fort Vermilion.

Disaster relief funding for punishing spring floods is welcome news to civic leaders in northern Alberta.

A longstanding battle between an Alberta entrepreneur and a $50-million lottery winner is still raging after a new legal judgment, a securities investigation, allegations of harassment and even duelling ebooks.

In a new twist on oral history, University of Alberta paleontologists have discovered that an Argentinian reptile from 95 million years ago developed a type of tooth enamel that is common in humans and other mammals but rare among reptiles.

The NDP health critic says comments Premier Doug Ford made this week calling out medical officers of health for not getting enough COVID-19 testing done are uncalled for.

Before COVID-19 hit, Hemliss Eloïse Konan had plans for how she'd spend her summer in Sudbury. After finishing her first year at Laurentian University, Konan planned to stay in residence, and get a job for the summer.

Tensions are rising on Manitoulin Island because a First Nation is stopping travellers on provincial highways that go through the community. But opinions on M'Chigeeng's attempt to protect its people from COVID-19 are not divided along racial lines. 

Three residents at a long-term care facility in Sault Ste. Marie have tested positive for COVID-19, prompting officials to declare an outbreak at Extendicare Maple View.

A doctor on Manitoulin Island is encouraging seasonal residents to stay home and not to come visit during the COVID-19 pandemic.

A new study facilitated by the Workplace Safety and Insurance Board of Ontario states miners who were forced to inhale an aluminum powder were found to have a higher risk of Parkinson’s disease.

The Porcupine Health Unit is reporting the fifth COVID-19 related death in its area.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

The Porcupine Health Unit is declaring a COVID-19 outbreak at a long-term care home in Iroquois Falls.

A former miner who was forced to breathe in a fine aluminum dust before each shift says he’s not surprised a new report links that dust and a higher risk of Parkinson’s disease.

Those in Sudbury may have had a few choice words when waking up Friday morning after a snowfall overnight.

The City of Timmins says a COVID-19 outbreak remains in place at a long-term care home in the city, even after the one affected resident has now tested negative — twice.

The head of a Sudbury-based book publishing company says she’s shifting launches online due to COVID-19.

The following is a guest post by Martha H. Kennedy, Curator of Popular & Applied Graphic Art, Prints & Photographs Division. During Will Eisner Week, March 1-7, 2020, the Library of Congress joins art schools, libraries, universities, and museums in a global celebration of this legendary creator’s contributions to the world of comic art and […]

Every year Washingtonians are treated to a feast for the eyes as ornamental cherry trees bloom across the city, most prominently by the Tidal Basin. As cherry blossom season approaches, we would like to share information about two related resources that we hope will offer some inspiration for those near and far: a selected set […]

Just across town from the Library of Congress in Washington, D.C., stands Gallaudet University, an institute for higher learning for the deaf and hard-of-hearing. In 1864, President Abraham Lincoln signed the law that allowed the school to begin issuing college degrees, a milestone for deaf people seeking higher education. Edward M. Gallaudet (right) was the […]

The following is a guest post by Hanna Soltys, Reference Librarian, Prints & Photographs Division. The post was written with the help of Sara W. Duke, Curator of Popular and Applied Graphic Art.   While professional baseball’s Opening Day will take place at a later date, the spirit and excitement of the day still live […]

Spring has arrived. While some of us may have an opportunity to carefully step outside and view blossoms in our own neighborhood, others may not. Wherever you are, you can take a virtual stroll among the shoots and blossoms planted among the collections of the Prints & Photographs Division. Many images of gardens can be […]

The following is a post by Kristi Finefield, Reference Specialist in the Prints & Photographs Division, and member of the Picture This blog team. Images have a way of opening our eyes to new aspects of a well-known story. When I think of singer Marian Anderson, an image of her performing at the Lincoln Memorial […]

Visitors to the 1900 Paris Exposition would have had the opportunity to view an extraordinary display of photographs, charts, publications and other items meant to demonstrate the progress and resilience of African Americans in the United States, only a few decades after the abolition of slavery. The materials were assembled by African American intellectuals Thomas J. […]

The following is a guest post by Katherine Blood, Curator of Fine Prints, Prints & Photographs Division. As the Library of Congress marks its 220th year of serving the nation, the publication of a new guide tells two stories: how staff have for decades worked with art professionals to build the collections and how by […]

The following is a post by Kristi Finefield, Reference Specialist in the Prints & Photographs Division, and member of the Picture This blog team. As the Library of Congress marks its 220th year, we take the opportunity to explore one example of its efforts to sustain and celebrate the arts in its physical spaces. Above […]

Below is an interview with Elizabeth Lindqwister, the summer 2019 Liljenquist Family Fellow, and Prints & Photographs Division staff members, Karen Chittenden and Micah Messenheimer, about creating a Story Map focusing on the Civil War experience of Susie King Taylor. Many courageous people are pulling double and triple duty in this time of quarantine for […]

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.

Over many years, the Xenopus laevis embryo has provided a powerful model system to investigate how mechanical forces regulate cellular function. Here, we describe a system to apply reproducible tensile and compressive force to X. laevis animal cap tissue explants and to simultaneously assess cellular behavior using live confocal imaging.