Monday marks the first Remembrance Day ceremony at the National War Memorial in St. John's to include the tomb of the Unknown Soldier. We're proud to present again two documentaries that tell a remarkable story.

The A.C. Hunter Public Library wants people in St. John’s to know that it’s open and accessible, despite how the entrance looks. The main staircase is under repair but there’s plenty of signs directing folks to other entrances. Still, librarians say people are judging this book by its cover and assuming the library is closed.

A Labrador foster parent and head of a group working to better the Labrador foster care system is stressing the importance of keeping Indigenous children with Indigenous parents in their Indigenous communities.

A researcher studying the rise of extremism in Atlantic Canada warns Ku Klux Klan Halloween costumes are just one example of an insidious effort by far-right groups to normalize hateful attitudes.

A candidate for the Nova Scotia NDP has apologized and is no longer running for the party in the provincial election after her comments on Israel and the Holocaust drew concern from the Jewish organizations. 

Volunteers will be cutting hair at a salon based inside a Cape Breton food bank. The goal is to provide clients with the same salon experience paying customers would receive.

Independent Elizabeth Smith-McCrossin is back on the campaign trail in Cumberland North, but opponents say the riding would be better represented by an MLA from an official party. 

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

As Nova Scotia politicians make their fall election promises, Julie Leggett is urging them to commit to helping people on income assistance stay cool during heat waves.

As Week 3 of the provincial election campaign begins, there are questions about how Nova Scotia's major political parties plan to combat the rising number of opioid overdose deaths. 

Building highways isn't the only solution to the growing problem of traffic congestion in and around the Halifax Regional Municipality, says the head of an organization that advocates for rail-based public transit.

The new mayor and council are planning big changes for the Cape Breton Regional Municipality, and one of those is at the top of the staff organizational chart now that chief administrative officer Marie Walsh is set to retire.

Elections Nova Scotia is investigating PC candidate Susan Corkum-Greek's campaign to determine whether it broke the law by handing out gift cards on Saturday outside a Tim Hortons in Lunenburg, N.S. 

The Société de transport de Montréal (STM) has established a non-urgent text line for transit users to signal security issues in the Metro network.

Loved ones, friends, and people from across the city have all come together to support each other at Pastel Rita, the café Jocelyn Després helped so many call home.

Amid rapid global advances and deployment of artificial intelligence technologies, the federal government has invested millions to combine the minds of three existing institutes into one that can keep an eye on potential dangers ahead.

Genetically encoded calcium indicators (GECIs) allow for the noninvasive evaluation of neuronal activity in vivo, and imaging GECIs in Drosophila has become commonplace for understanding neural functions and connectivity in this system. GECIs can also be used as read-outs for studying sleep in this model organism. Here, we describe a methodology for tracking the activity of neurons in the fly brain using a two-photon (2p) microscopy system. This method can be adapted to perform functional studies of neural activity in Drosophila under both spontaneous and evoked conditions, as well as during spontaneous or induced sleep. We first describe a tethering and surgical procedure that allows survival under the microscopy conditions required for long-term recordings. We then outline the steps and reagents required for optogenetic activation of sleep-promoting neurons while simultaneously recording neural activity from the fly brain. We also describe the procedure for recording from two different locations—namely, the top of the head (e.g., to record mushroom body calyx activity) or the back of the head (e.g., to record central complex activity). We also provide different strategies for recording from GECIs confined to the cell body versus the entire neuron. Finally, we describe the steps required for analyzing the multidimensional data that can be acquired. In all, this protocol shows how to perform calcium imaging experiments in tethered flies, with a focus on acquiring spontaneous and induced sleep data.

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

On this episode of Generation Anthropocene, we dive into one of the most underappreciated elements on the periodic table

Food is a focal point for understanding broader environmental problems. In this podcast, we learn how food buyers are influenced in surprising ways.

An unlikely friendship with Guy Burgess, the infamous British double-agent, brought unexpected joy to Stanley Weiss

In opening remarks at the Inuit-Crown partnership committee meeting in Ottawa on Friday, Crown-Indigenous Relations Minister Gary Anandasangaree said the government is preparing to deliver an apology in Nunavik — the Inuit region of northern Quebec.

With president-elect Donald Trump promising to drill in Alaska's Arctic National Wildlife Refuge, the Gwich'in are preparing to fight for the Porcupine caribou herd.

Yukon appeal court Justice Karen Horsman granted a temporary stay of the requirement on Friday, the latest development in an ongoing legal battle over the property between the territorial government, cabin-owner Len Tarka and tenant Eric DeLong. 

FIFA Football for Schools (F4S) has come to Canada, with a pilot program in the N.W.T. already underway and offering coach training. 

Historians say that Sarah Emma Edmonds exaggerated many aspects of her wartime experiences. Still, she bravely served in the Union Army, becoming one of hundreds of women who fought in the conflict in secret

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

In the early 1990s, historians and the public alike questioned how Disney's America would accurately and sensitively document the nation's thorny past

The British explorer named dozens of geographical features and sites in the region, ignoring the traditions of the Indigenous peoples who’d lived there for millennia

In the Jim Crow South, activists became martyrs at the hands of white racists, all for the just cause of using the vote to fight for equality and freedom

Before fitness influencers made getting your steps in a trend, pedestrianism had the nation on their feet

Out-of-the-can branding helped transform World War II's rations into a beloved household staple

Advocates say pay equity laws, while needed, often do not help the most vulnerable workers.

There have been 25 confirmed measles cases in New Brunswick since October after the identification of 11 new cases, according to a news release Saturday from Public Health.

Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

Eight years ago, a woman filed a complaint about two RCMP constables in Moncton. She accused them of having "an improper and racist attitude" and of discriminating against her and her husband during an investigation into a stolen truck and trailer. 

The University of Regina Rams knock off their provincial rivals, the University of Saskatchewan Huskies to win their second ever Hardy Cup championship. An emotional Rams head coach, Mark McConkey talks about how his team was able to overcome a rough regular season and win the title.

Inflation, an increase to the debt limit, and a series of mega-projects have been focuses of the municipal election in Regina.

Families are grappling with the consequences of growing gun violence in Hamilton and across the Greater Toronto Area this year. Several of the region's police forces have warned of an alarming rise in shootings since January.

The union for wildland firefighters says a recently passed Ontario bill that makes them eligible for presumptive cancer care under the Workplace Safety and Insurance Board doesn't go far enough.

Dave Campbell of Thunder Bay, Ont., is resting his legs after taking the ride of his life through the Western Cape of South Africa in the 800-kilometre bikepacking 'Rhino Run.' Here are some of the photos he shared from his six-day expedition at the end of October.

Wildlife photographer Jason Leo Bantle recounts the moment he came across Banff’s most famous grizzly ‘The Boss,’ during the bear’s surprise visit to Canmore, Alta.

Buenos Aires, Argentina :: Miembros de la comunidad japonesa encuentran amigos en un barco de visita y escuchan el evangelio en su lengua materna.

A South East Asian OM worker shared about how her relationship with Jesus has changed her life from one of fear to leading others and modeling Christ's love.

La tripulación de técnicos del Logos Hope sabe que el mantenimiento del barco es una forma de hacer ministerio.

Roughly 30 percent of Catalonia is protected land making it a stunning place to hit the trails

At 2,700 square kilometres, CFB Suffield is the largest military training area in Canada. It has been the site of military training in southern Alberta since 1972.

An intimate look at the lessons and legacy of Murray Sinclair, chair of the Truth and Reconciliation Commission, and how he changed Canada.

Small businesses are keeping a close eye on the possibility of job action as they stock their shelves for Black Friday and holiday shopping.