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The post REPORT: Airline to suspend service to nearly a dozen US airports starting next week appeared first on Dennis Michael Lynch.

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The post REPORT: Three Times Joe Biden Was Reportedly Involved with the Russia Collusion Hoax appeared first on Dennis Michael Lynch.

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The following article, California Sheriff Refuses to Arrest People Defying Stay-at-Home Order: “There cannot be a new normal”, was first published on 100PercentFedUp.com.

Riverside, California Sheriff Chad Bianco spoke to the Riverside Board of Supervisors on May 5th to say that he will not enforce the stay-at-home order in California. He tells people who are afraid of contracting the coronavirus that they should stay home if they want to. Bianco continues with the suggestion that any business owner […]

Continue reading: California Sheriff Refuses to Arrest People Defying Stay-at-Home Order: “There cannot be a new normal” ...

The following article, Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “, was first published on 100PercentFedUp.com.

Now that Michael Caputo, who was previously a target of the Mueller investigation, has been cleared, he has released some pretty damning text messages from the virulently anti-Trump lawmaker from California, Rep. Eric Swalwell (D). The text messages appear to prove that Swalwell, who has spent the last 3 1/2 years calling for Trump’s impeachment, […]

Continue reading: Witness Tampering? Asst. HHS Secretary Releases Threatening Text Messages From Dem Rep. Eric Swalwell: “In clear violation of House Ethics rules “ ...

20 November 2013

7 October 2014

Members Event

Event participants

Bruce Stokes, Director, Global Economic Attitudes project, Pew Research Center; Associate Fellow, Americas Programme, Chatham House
Roderic Wye, Associate Fellow, Asia Programme, Chatham House
Dr Alexandra de Hoop Scheffer, Senior Transatlantic Fellow and Director, Paris Office, German Marshall Fund of the United States 
Chair: Dr Robin Niblett, Director, Chatham House 

Research Event

Event participants

Dr Doyin Okupe, Senior Special Assistant on Public Affairs to the President of Nigeria 

Invitation Only Research Event

Event participants

Ambassador Linda Thomas-Greenfield, Assistant Secretary of State for African Affairs, US Department of State
Chair: Dame Rosalind Marsden, Associate Fellow, Africa Programme, Chatham House

Research Event

Event participants

Jeremy Gordon, Director, China Business Services
Chair: Roderic Wye, Associate Fellow, Asia Programme, Chatham House

27 January 2015

21 July 2016

Ousted vaccine expert Rick Bright said he is "frustrated at our inability to be heard as scientists."

Elizabeth Spiers wrote about an incident where Jared Kushner used the memorial of an employee to congratulate himself for success.

GAZIANTEP, Turkey—After five years fighting to preserve Bashar al-Assad’s regime in Syria, Russia now appears inclined to dispose of its infamous client. Assad’s persistent brutality and corruption, and his inability to establish even the semblance of a functioning state, has grown to be a burden Moscow would prefer not to bear.And then there’s the problem of Iran. Assad, members of his family, and his Alawite clansmen enjoy close, perhaps unbreakable, bonds to the regime in Tehran and to Iranian-backed militias in Syria. All of which undermines Moscow’s primary mission there: to rehabilitate the Assad regime as a symbol of stability capable of attracting hundreds of billions of dollars of foreign investment for reconstruction, which Russian firms would then be poised to receive. As long as Assad’s relatives continue to function as a mafia and give free rein to Iranian troops using Syria as base of operations to threaten Israel and plan attacks against U.S. troops in Iraq, those countries likely to foot the bill for Syrian reconstruction—the nations of Europe and the Gulf—are unlikely to come up with the cash. Amid Escalating Syrian Carnage, Turkey Shoots Down Assad’s PlanesThis has not gone unnoticed by the United States.“Assad has done nothing to help the Russians sell this regime,”James Jeffrey, the U.S. special envoy for the Coalition to Defeat ISIS, told reporters in a State Department briefing on Thursday. “You find Assad has nothing but thugs around him, and they don't sell well either in the Arab world or in Europe. We have heard repeatedly from Russians we take as credible that they understand how bad Assad is.” The Syrian president’s “refusal to make any compromises” in order to secure diplomatic recognition and acceptance for his regime has jeopardized “hundreds of billions of dollars in reconstruction assistance” for Syria, according to Jeffrey. Yet the Trump administration is unlikely to exploit this growing rift. “Getting Russia out of Syria,” Jeffrey said, “has never been our goal. Russia has been there for 30 years. It has a long-term relationship with Syria. We don’t think it has been healthy for the region. We don’t think it really is even healthy for Russia. But that’s not our policy.”  MEDIA FRENZYJeffrey’s statements come just one week after Russian state media unleashed a slew of reports and editorials targeting Assad, portraying the beleaguered president as hopelessly corrupt and unfit to govern, and suggesting the time had come to replace him with a new leader.The first batch of articles was published by the Russia’s Federal News Agency (FNA), an outlet owned by Yevgeny Prigozhin, a Russian oligarch and chairman of several companies implicated in the 2016 U.S. elections scandal. Appearing over the course of a mere three hours on April 17, they would shake Syria to its core. The first of the three articles in question highlighted a corruption scheme carried out by the regime in summer 2019 in which the Syrian prime minister purportedly lied to citizens about oil and gas scarcities in order to justify the occurrence of long power outages while selling Syrian electricity to businessmen in Lebanon. The second piece cited an opinion poll claiming only 32 percent of Syrians would vote for Assad in the country’s upcoming 2021 presidential election. The third and final article, entitled, “Corruption is Worse than Terrorism,” chastized President Assad for personally failing to combat corruption, prevalent at all levels of the state.  That these were published by Prigozhin’s news agency was the kind of signal it would be hard for Assad to miss. Prigozhin, who first built his fortune as a caterer, is sometimes known as “Putin’s chef.” But of particular relevance to Syria is his role as chairman of the Wagner Group, whose mercenaries have fought alongside Assad regime forces since October 2015 and helped the latter take back control of key revenue generating infrastructure such as the al-Sha’ir gas field in Homs province.Deputy Assistant Secretary Christopher Robin told the same State Department briefing Thursday, “Wagner is often misleadingly referred to as a Russian private military company, but in fact it’s an instrument of the Russian government which the Kremlin uses as a low-cost and low-risk instrument to advance its goals.”The article on corruption would also point out, suggestively, that the Assads are not the only powerful family in Syria, “there are also the Makhloufs.”Rami Makhlouf, who is in fact Bashar al-Assad’s first cousin, is Syria’s wealthiest man, and also, it would seem, Russia’s man. Certainly he has strong ties to the Kremlin and for years has been one of the most vocal critics of Iran’s presence in Syria. In July 2018, the al-Watan newspaper, one Syria’s most prominent pro-regime mouthpieces and owned by Rami Makhlouf since 2006, published a then unprecedented public rebuke to Iran, accusing it of sponsoring Islamist fanaticism throughout the Middle East alongside Turkey and Qatar, the main backers of Syria’s opposition. (Rami Makhlouf’s father Muhammad and brother Hafiz meanwhile are alleged by some to be living in Russia.) The April 17 articles published by Prigozhin’s FNA preceded the release of a wave of other articles and items in the media over the next 12 days that would further drive home the point that Moscow was considering options other than Assad to rule Syria. TASS, Russia’s largest state-run news agency, wrote in one editorial that, “Russia suspects that Assad is not only unable to lead the country anymore, but also that the head of the Syrian regime is dragging Moscow towards the Afghani scenario.” This is like evoking the Vietnam War for an American audience, a reference to the Kremlin’s botched campaign through the 1980s that helped bankrupt the Soviet Union and finally break it apart.Amid this coverage, TASS would also take swipes at Iran, claiming that the Islamic Republic has “no interest in achieving stability in the region, because it considers it a battlefield with Washington”.On April 30, the Russian International Affairs Council (RIAC), a think tank established by Moscow’s Ministry of Foreign Affairs, released a scathing report saying Russia was in talks with other parties to the Syrian conflict to draw up plans for a political resolution that did not include Bashar al-Assad as president. The report highlighted purported Russian efforts to compel the Syrian regime to commit to ceasefires with both American-backed and Kurdish-led Syrian Democratic Forces (SDF), and the Turkish-backed Free Syrian Army (FSA) opposition, while beginning steps to form a new unity government that would include representatives from both. That day, Rami Makhlouf, whose assets were frozen five months earlier as part of a tax dispute, uploaded a video onto his personal Facebook page accusing the Assad regime of corruption. In a state known for carrying out the full-scale slaughter of those who test its authority, Makhlouf’s videos, coming on the heels of the unprecedented Russian attacks in the media, sent shockwaves throughout the country.  THE ROYAL FAMILYWhile the Makhlouf clan clearly has thrown its lot in with Russia, key members of Bashar al-Assad’s immediate family and others with ties to Qardaha in Syria’s largely Alawite Latakia province, are among the most prominent Iranian-backed militia leaders in Syria. It’s an alliance that traces back to his father Hafez al-Assad, who was born in Qardaha, and who forged ties with the Iranian revolution almost from its beginning more than 40 years ago. The Iranians responded by offering religious legitimacy to the Alawite sect, which is regarded as heretical by Sunnis and indeed by many Shi’a.These Qardaha militia leaders have regularly engaged in armed clashes against Russian backed units. They are among the most egregious violators and abusers of power, overseeing wide networks of corruption similar to those lamented in the Russian media. And foremost among them is Bashar’s younger brother, Maher al-Assad. Since April 2018, Maher al-Assad has commanded the Syrian Army’s 4th Armored Division, one of country’s oldest, best equipped and overwhelmingly Alawite brigades. After the 2011 outbreak of the Syrian revolution, when the loyalty of much of the army was in doubt, it became a refuge for numerous Alawite-Shi’a dominated pro-regime militias.Currently, the 4th Armored Division’s members control many smuggling operations throughout the country, in cities from Albu Kamel on Syria’s eastern border with Iraq to Latakia on the Syrian coast, where the port was leased to Iran on October 1 last year. It has since become one of the biggest export hubs for drugs headed to markets in Europe, the Middle East and North Africa. Examples abound: On July 5, 2019, Greek coast guard and drug enforcement officials announced the biggest drug bust in history, seizing 5.25 tons (33 million pills) of Captagon amphetamines worth $660m hidden in shipping containers loaded at the Latakia port in Syria. That followed a long string of such seizures made by Greek authorities. More recently, in late April, customs officials in both Saudi Arabia and Egypt also announced the seizure of similar quantities of drugs in containers traced back to Latakia. Local reports have accused a range of actors including Maher al-Assad’s 4th Division, Hizbollah, Rami Makhlouf, and others of profiting from the massive drug exports emanating from the port. In January 2019 the 4th Armored Division launched attacks on the Russian-backed Tiger Forces unit in an attempt to wrest control of smuggling routes between regime- and opposition-held territory in Idlib province. The clashes led to the death of 70 fighters. These and other skirmishes prompted Russia to back a major campaign to arrest 4th Division and other Iranian-backed units throughout the country beginning in April 2019, which succeeded in rounding up numerous mid-ranking Iranian-backed officers. Among those targeted in the campaign was Bashar Talal al-Assad, a cousin to the president (similar name, different people) who was wanted on drug and weapons trafficking charges. Unlike others who were detained in the roundup, Bashar Talal al-Assad and his ‘Areen Brigade managed to fight off Russian-backed forces that sought to arrest him in Qardaha. He then pledged to attack Russia’s Hmeimim military base, located 17 miles east of Latakia city, in the event the regime sought to arrest him again.For Russia, the threat of such attacks on its military infrastructure is a real concern. The Hmeimim base—from which Moscow has directed its entire military campaign in Syria—had already been subject to a series of attacks from January to October 2018 by other Iranian-backed militias in the area. The threat posed by both Iran’s acquisition of the Latakia port and its support for local Assad family proxies in Syria’s coastal region is exacerbated by the fact that Tehran has also begun making progress toward completing construction of its Shalamcha railroad, which, via stops in Basra, Baghdad, Albu Kamel and Damascus, will give Tehran direct access to the Syrian and Lebanese coasts. If Iran succeeds in integrating the Latakia port with the Shalamcha rail line, this will cut off Hmeimim from Russian forces in central and southern Syria and enable Tehran to quickly deliver weapons to proxy forces in Latakia that are already engaged in clashes against Russian-backed groups. WORLDWIDE CONSENSUSMoscow’s inability to control Iranian backed Syrian militiamen engaged in widespread crime, corruption, and assaults on Russian forces has infuriated the Kremlin. But Russia is not the only major player on the ground with scores to settle against Iran, and the Russian military leadership in Syria has ignored if not largely encouraged Israeli strikes on Iranian troops throughout the country.It may not be coincidental that the Israeli attacks have increased in pace and scope since April, following the flurry of Russian media articles attacking Assad and his regime. “We have moved from blocking Iran’s entrenchment in Syria to forcing it out of there, and we will not stop,” Israel’s new defense minister, Naftali Bennett, declared on April 28. Without Russia, Iran has found itself the odd man out in Syria, the single party still seeking to push for war at a time when most other international players have been struck with fatigue and simply seek to put Syria’s pieces back together. President Recep Tayyip Erdoğan of Turkey, the last patron of Syria’s battered FSA opposition, has himself made peace with Moscow, effectively agreeing last March to cede control of wide swaths of rebel held territory after a particularly bloody Russian led campaign against the last FSA holdout in Idlib province that ended in victory for regime forces. Ironically, Erdoğan’s long-held desire to overthrow Syria’s president may still come to fruition, albeit not as he expected, as Assad’s ouster may come at the hands of Russia itself, and not the revolution. Read more at The Daily Beast.Get our top stories in your inbox every day. Sign up now!Daily Beast Membership: Beast Inside goes deeper on the stories that matter to you. Learn more.

Whenever Americans see videos of police brutality against black men and women, the first thing they do is assume they deserved their executionWhat skin color are the bad guys in America’s fantasies of vigilantism? When the proverbial “fellas” get together to drink beers and talk about their newest guns and who they’d take down, what race are the “criminals” in the theater of their minds?When Greg McMichael and his son, Travis, got the call from their neighbor that a “burglar” was running through their Brunswick, Georgia neighborhood that chilly February day, what color man do you think they imagined as they locked, loaded, and embarked on their “mission”?Ahmaud Arbery is dead today because when Americans dream of vigilante justice, black men are the villains of their imaginations.We as a nation are so comfortable with this baseline bigotry that our first assumption whenever we see videos of police brutality against or shootings of black men and women, the first thing we do is assume that the victims must have done something wrong to earn their own public execution.This assumption is both a function of white America having a completely different experience with police officers than black America as well as the hundreds of years of vilifying blackness in media and American culture.I will never forget the biggest and most uproarious applause during the theater debut of the lackluster 2007 vigilante film, Brave One, came when the protagonist Jodi Foster got her first vigilante kills of the movie – two threatening and scary black men. That theater filled with men the same age range as Greg and Travis McMichael erupted as if at that moment, all that they had ever imagined had been fulfilled on the big screen. Needless to say, I left that theater before the credits rolled.Across the country, our political leaders hold these same bigoted beliefs which inevitably lead to policies that directly assume criminality based on skin color.During his tenure as mayor of New York City, billionaire Michael Bloomberg made it explicitly clear why it was that he sent police officers into black and brown communities to “throw them” up against the wall. In his 2015 Aspen Institute speech he stated:“People say, ‘Oh my God, you are arresting kids for marijuana who are all minorities.’ Yes, that’s true. Why? Because we put all the cops in the minority neighborhoods. Yes, that’s true. Why’d we do it? Because that’s where all the crime is. And the way you should get the guns out of the kids’ hands is throw them against the wall and frisk them.”And it is for this reason that I do not distinguish between the violence committed by American citizens acting as vigilantes and the violence committed by so-called officers of the law when, in both cases, the working assumption and driving force behind that violence is the deeply bigoted and firmly American association between blackness and criminality.For Ahmaud, that association not only led to his brutal killing, but it also initially meant his killer not being arrested. It took more than two months for the father and son duo to be arrested. When explaining why they were not charged immediately the district attorney, George Barnhill, immediately stated that the victim, Ahmaud Arbery, was, in fact, the “criminal suspect”.“It appears that [Greg and Travis McMichael’s] intent was to stop and hold this criminal suspect until law enforcement arrived. Under Georgia Law [sic] this is perfectly legal.”Even after viewing the video and with no evidence beyond Ahmaud’s skin color, the top cop in the institution designed to bring equal justice under the law concluded that Ahmaud was a criminal suspect when he was simply a black man taking a jog.What are black Americans to do when justice is delayed or outright denied because of the assignment of innocence to vigilantes and police officers?What are black Americans to do when the assumption of guilt because of our skin color is as American as the guns they use to kill us?What are we to do when in our neighbors’ dreams and fantasies of cop-and-robber, the skin color of the bad guy matches our own?The very first thing we are going to do is defend ourselves as if our lives depend on it because when Americans fantasize about killing, those fantasies become our living nightmares. * Benjamin Dixon is the host of the Benjamin Dixon show.

South Dakota Governor Kristi Noem sent letters to two Sioux tribes demanding they remove COVID-19 checkpoints because they interfere with traffic.

The White House has halted talks with Congress over any further coronavirus stimulus package as it waits for more information about how U.S. state reopenings affect the economy, White House top economic adviser Larry Kudlow told reporters on Friday. The Senate and House of Representatives have already passed four major bills to address the novel coronavirus outbreak, including three aimed at stabilizing the economy as most Americans have sheltered in place and unemployment has soared.

The former Senate staffer, who is accusing Joe Biden of sexually assaulting her 27 years ago, is being represented by a lawyer who is also a donor to President Trump.

"I think it's always important to think about suicide as individual vulnerabilities and context," Eric Caine said.

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The government said it's distributing the promising coronavirus drug, remdesivir, to some hard-hit states. Eventually, all 50 states should get it.

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Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8+ T-cell–mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I–β2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV–PA123–130. We determined the structure of the BF2*1501–PA123–130 complex at 2.1 Å resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123–130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123–130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8+ T cells specific for the HPAIV–PA123–130 epitope in peptide-immunized chickens indicated that a repertoire of CD8+ T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123–130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

β-1,3-d-Glucan is a ubiquitous glucose polymer produced by plants, bacteria, and most fungi. It has been used as a diagnostic tool in patients with invasive mycoses via a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no method is currently available for measuring β-1,6-glucan, another primary β-glucan structure of fungal polysaccharides. Herein, we describe the development of an economical and highly-sensitive and specific assay for β-1,6-glucan using a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase activity. The purified β-1,6-glucanase derivative bound to the β-1,6-glucan pustulan with a KD of 16.4 nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its ability to detect cell wall β-1,6-glucan from both yeast and hyphal forms of the opportunistic fungal pathogen Candida albicans, without any detectable binding to glucan lacking the long β-1,6-glucan branch. We developed a sandwich ELISA-like assay with a low limit of quantification for pustulan (1.5 pg/ml), and we successfully employed this assay in the quantification of extracellular β-1,6-glucan released by >250 patient-derived strains of different Candida species (including Candida auris) in culture supernatant in vitro. We also used this assay to measure β-1,6-glucan in vivo in the serum and in several organs in a mouse model of systemic candidiasis. Our work describes a reliable method for β-1,6-glucan detection, which may prove useful for the diagnosis of invasive fungal infections.

Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers.

This report describes the range of policies available to improve immigrants’ economic integration through language acquisition, especially those focused on getting immigrants into jobs or moving into higher-paying jobs. It assesses promising models and practices from Europe and North America.