A researcher studying the rise of extremism in Atlantic Canada warns Ku Klux Klan Halloween costumes are just one example of an insidious effort by far-right groups to normalize hateful attitudes.

Volunteers will be cutting hair at a salon based inside a Cape Breton food bank. The goal is to provide clients with the same salon experience paying customers would receive.

Kate MacEachern is a Canadian veteran from Ballantynes Cove, N.S., but her service years are far from over. She has spent about two years living in Ukraine, delivering humanitarian aid to communities across the country.

As Week 3 of the provincial election campaign begins, there are questions about how Nova Scotia's major political parties plan to combat the rising number of opioid overdose deaths. 

Facing soaring food prices and an escalating housing crisis, Quebec student associations are urgently calling on the provincial government to fund internships required for post-secondary training.

The union representing dockworkers in Montreal says Canada's labour minister forcing its members back to work at ports in Montreal and Quebec City marks a "dark day for workers' rights."

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

Genetically encoded calcium indicators (GECIs) allow for the noninvasive evaluation of neuronal activity in vivo, and imaging GECIs in Drosophila has become commonplace for understanding neural functions and connectivity in this system. GECIs can also be used as read-outs for studying sleep in this model organism. Here, we describe a methodology for tracking the activity of neurons in the fly brain using a two-photon (2p) microscopy system. This method can be adapted to perform functional studies of neural activity in Drosophila under both spontaneous and evoked conditions, as well as during spontaneous or induced sleep. We first describe a tethering and surgical procedure that allows survival under the microscopy conditions required for long-term recordings. We then outline the steps and reagents required for optogenetic activation of sleep-promoting neurons while simultaneously recording neural activity from the fly brain. We also describe the procedure for recording from two different locations—namely, the top of the head (e.g., to record mushroom body calyx activity) or the back of the head (e.g., to record central complex activity). We also provide different strategies for recording from GECIs confined to the cell body versus the entire neuron. Finally, we describe the steps required for analyzing the multidimensional data that can be acquired. In all, this protocol shows how to perform calcium imaging experiments in tethered flies, with a focus on acquiring spontaneous and induced sleep data.

Sleep studies in Drosophila melanogaster rely mostly on behavioral read-outs to support molecular or circuit-level investigations in this model. Electrophysiology can provide an additional level of understanding in these studies to, for example, investigate changes in brain activity associated with sleep manipulations. In this protocol, we describe a procedure for performing multichannel local field potential (LFP) recordings in the fruit fly, with a flexible system that can be adapted to different experimental paradigms and situations. The approach uses electrodes containing multiple recording sites (16), allowing the acquisition of large amounts of neuronal activity data from a transect through the brain while flies are still able to sleep. The approach starts by tethering the fly, followed by positioning it on an air-supported ball. A multichannel silicon probe is then inserted laterally into the fly brain via one eye, allowing for recording of electrical signals from the retina through to the central brain. These recordings can be acquired under spontaneous conditions or in the presence of visual stimuli, and the minimal surgery promotes long-term recordings (e.g., overnight). Sleep and wake can be tracked using infrared cameras, which allow for the measurement of locomotive activity as well as microbehaviors such as proboscis extensions during sleep. The protocol has been optimized to promote subject survivability, which is an important factor when performing long-term (~16-h) recordings. The approach described here uses specific recording probes, data acquisition devices, and analysis tools. Although it is expected that some of these items might need to be adapted to the equipment available in different laboratories, the overall aim is to provide an overview on how to record electrical activity across the brain of behaving (and sleeping) flies using this kind of approach and technology.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

After more than a century of searching, scientists have discovered a gene in snails that may control asymmetries inside many animals

A Two-Day Festival in the historic Arts & Industries Building brings community, artists and scholars together for a “Culture Lab”

An unlikely friendship with Guy Burgess, the infamous British double-agent, brought unexpected joy to Stanley Weiss

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

More than 50 Norman Wells residents gathered at the Royal Canadian Legion on Thursday night to voice frustrations over high heating fuel prices.

Tammy Roberts, Home Base's executive director, said before these services launched, there was "no place for youth to go" other than the emergency room or to RCMP cells. 

With president-elect Donald Trump promising to drill in Alaska's Arctic National Wildlife Refuge, the Gwich'in are preparing to fight for the Porcupine caribou herd.

Paraglider Ben Lewis remembers spinning around wildly as he got pulled up higher and higher into the violent storm. That was when he realized, about 6,700 metres up in the sky and still rising, that he was not likely to see his family again.

Hay River RCMP say they believe two weekend incidents, including one in which a person was shot, are linked to drugs and organized crime.

A group of volunteers have been providing emergency services to West Dawson residents during the periods of freeze up and break up, when there is no reliable access across the Yukon River to town.

A man who fled from police, drove onto a walking trail, triggered a shelter-in-place advisory for the Chateau Nova hotel in Yellowknife and subsequently broke into a home was on bail at the time and under house arrest, police say.

Historians say that Sarah Emma Edmonds exaggerated many aspects of her wartime experiences. Still, she bravely served in the Union Army, becoming one of hundreds of women who fought in the conflict in secret

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

In the early 1990s, historians and the public alike questioned how Disney's America would accurately and sensitively document the nation's thorny past

Fifty years ago, on October 5, 1974, David Kunst completed the first verified circumnavigation of the globe on foot. Along the way, he met Princess Grace of Monaco, raised money for UNICEF and lost a brother to bandits

An exhibition at the Huntington Library, Art Museum and Botanical Gardens explores how Western intellectuals viewed the climate crisis between 1780 and 1930

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation

With flinty perseverance and a golden touch, Belinda Mulrooney earned an unlikely fortune in the frozen north and reshaped the Canadian frontier

A new exhibition at the British Library explores the public, private and spiritual lives of such figures as Joan of Arc, Christine de Pizan and Hildegard of Bingen

The Sacro Bosco's meaning is the subject of debate, with scholars alternatively describing the sprawling complex as a memorial, an allegorical site or a tribute to ancient civilizations

In the Jim Crow South, activists became martyrs at the hands of white racists, all for the just cause of using the vote to fight for equality and freedom

Before fitness influencers made getting your steps in a trend, pedestrianism had the nation on their feet

A Romare Bearden print served as a starting point for the American playwright's 1987 drama, which follows a Black family's struggle to decide the fate of an ancestral heirloom

Out-of-the-can branding helped transform World War II's rations into a beloved household staple

A jury has found a 20-year-old Moncton man guilty of second-degree murder in the 2022 death of an 18-year-old.

There have been 25 confirmed measles cases in New Brunswick since October after the identification of 11 new cases, according to a news release Saturday from Public Health.

Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

From bravery on the field of battle to stoicism behind the lines, New Brunswickers of all backgrounds made their marks.

Emergency officials said the fire at the Herring Pub and Restaurant on Water Street broke out around 4 p.m. and appears to have started in an air conditioning unit.   

Alheri Bingi moved to Canada from Lagos four years ago. Noticing a lack of representation of her Nigerian culture, Bingi started creating batik textile art using authentic African fabric.

Eight years ago, a woman filed a complaint about two RCMP constables in Moncton. She accused them of having "an improper and racist attitude" and of discriminating against her and her husband during an investigation into a stolen truck and trailer. 

New Brunswick’s Court of Appeal has reserved judgment in the case of a man found guilty of criminal negligence in the death of Fredericton teenager in 2018.

Inflation, an increase to the debt limit, and a series of mega-projects have been focuses of the municipal election in Regina.

Families are grappling with the consequences of growing gun violence in Hamilton and across the Greater Toronto Area this year. Several of the region's police forces have warned of an alarming rise in shootings since January.

With Remembrance Day and Indigenous Veterans Day coming up, many people start to reflect on their family's wartime stories by looking through mementos saved by former generations. Ron Kanutski of Thunder Bay, Ont., joins the CBC's Mary-Jean Cormier to talk about his family's military history.

Even as they fundraise for other community organizations, some legions across Canada are having trouble keeping their own lights on amid rising inflation and maintenance costs.

The union for wildland firefighters says a recently passed Ontario bill that makes them eligible for presumptive cancer care under the Workplace Safety and Insurance Board doesn't go far enough.

Ginoogaming First Nation has declared a state of emergency after an engineering inspection showed the bridge leading to the community should be closed to traffic.

Since 1970, Lakehead Public Schools has operated Kingfisher Lake Outdoor Education Centre just outside of Thunder Bay, Ont. The CBC's Matt Fratpietro spent a morning with students from École Elsie MacGill Public School to learn more about the forests that surround the city.

Hospice Northwest Services is inviting people to visit its third annual photography exhibit, "A Personal Lens on Grief." Here's what the project in Thunder Bay, Ont., entails, and why staff say it's important to find creative ways to express loss.