LOCKDOWN measures could turn the UK into "postcode lottery" as police forces admit they will not pursue low level speeding offences amid the restrictions.

MARTIN LEWIS has urged motorists to maintain their car in a good condition to prevent drivers from "shelling out" for repairs once the coronavirus lockdown is lifted.

LOCKDOWN measures have broken motorists' "car dependency" habits and the changes could even be "permanent" for many after restrictions have lifted, according to an expert.

Premier League clubs will disinfect the grass when the season resumes.

BORIS Johnson's deputy chief medical officer has warned the next coronavirus lockdown steps could prove to be extremely painstaking.

BORIS JOHNSON is set to announce a 14-day quarantine for travellers into the UK to avoid a second coronavirus peak - but should the Prime Minister have brought in the measure before now?

BORIS Johnson's deputy chief medical officer has warned the next coronavirus lockdown steps could prove to be extremely painstaking.

Premier League clubs will disinfect the grass when the season resumes.

On October 2, 2019, Express.co.uk published an article headlined "Farage picks apart ‘totally dishonest' Boris speech with £1.8 trillion debt warning" that originally claimed Nigel Farage had "laid into the Prime Minister after Boris Johnson had reportedly claimed the Conservative government had ‘wiped out the national debt'" in a speech at the Conservative Party Conference."

MIRIAM MARGOLYES sparked outrage after the Harry Potter star admitted she wanted "Boris Johnson to die" of coronavirus after he was hospitalised during an on-air rant.

BORIS Johnson praised a 102-year old Second World War veteran as "awesome" yesterday during a VE Day video call.

BORIS Johnson will only unveil "limited" changes to coronavirus lockdown rules to come into effect next week, a Cabinet minister said yesterday.

GERMANY is worried about the possibility of a hard Brexit as trade negotiations between the UK and the EU have so far resulted in very little progress.

KEIR STARMER's secret weapon against Prime Minister Boris Johnson has been revealed by Professor Tim Bale who detailed what the Labour leader can succeed in.

ANN WIDDECOMBE has warned it is "crucial" Boris Johnson does not extend the transition period.

Premier League clubs will disinfect the grass when the season resumes.

IRANIAN agents are delivering suitcases stuffed with £800,000 in cash to Hezbollah terrorists despite the Islamic state begging for financial help to deal with coronavirus, it is claimed.

IT WAS achieved with almost military precision. A wall was breached and a platoon of trusted lieutenants and foot soldiers was unleashed on the nation, who were to perform brilliantly under continued fire.

Within an eight hour period, IMPD officers have been involved the fatal shooting of two suspects and the death of a pregnant pedestrian.

Within an eight hour period, IMPD officers have been involved the fatal shooting of two suspects and the death of a pregnant pedestrian.

The Food and Drug Administration authorises emergency use of the experimental anti-viral drug.

Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate. Examining fermentation extracts of Streptomyces conglobatus, here we discovered four new neoantimycin analogs, unantimycins B–E, in which 3-formamidosalicylates are replaced by an unusual 3-hydroxybenzoate (3-HBA) moiety. Unantimycins B–E exhibited levels of anticancer activities similar to those of the chemotherapeutic drug cisplatin in human lung cancer, colorectal cancer, and melanoma cells. Notably, they mostly displayed no significant toxicity toward noncancerous cells, unlike the serious toxicities generally reported for antimycin-type natural products. Using site-directed mutagenesis and heterologous expression, we found that unantimycin productions are correlated with the activity of a chorismatase homolog, the nat-hyg5 gene, from a type I PKS gene cluster. Biochemical analysis confirmed that the catalytic activity of Nat-hyg5 generates 3-HBA from chorismate. Finally, we achieved selective production of unantimycins B and C by engineering a chassis host. On the basis of these findings, we propose that unantimycin biosynthesis is directed by the neoantimycin-producing NRPS–PKS complex and initiated with the starter unit of 3-HBA. The elucidation of the biosynthetic unantimycin pathway reported here paves the way to improve the yield of these compounds for evaluation in oncotherapeutic applications.

Invitation Only Research Event

Event participants

Philip Rycroft, Permanent Secretary, Department for Exiting the EU (2017-19)
Chair: Tom Raines, Head, Europe Programme, Chatham House

13 December 2019

4 March 2020 , Volume 96, Number 2

Expert

Here's what we expect from the new government's IT policies

Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate. Examining fermentation extracts of Streptomyces conglobatus, here we discovered four new neoantimycin analogs, unantimycins B–E, in which 3-formamidosalicylates are replaced by an unusual 3-hydroxybenzoate (3-HBA) moiety. Unantimycins B–E exhibited levels of anticancer activities similar to those of the chemotherapeutic drug cisplatin in human lung cancer, colorectal cancer, and melanoma cells. Notably, they mostly displayed no significant toxicity toward noncancerous cells, unlike the serious toxicities generally reported for antimycin-type natural products. Using site-directed mutagenesis and heterologous expression, we found that unantimycin productions are correlated with the activity of a chorismatase homolog, the nat-hyg5 gene, from a type I PKS gene cluster. Biochemical analysis confirmed that the catalytic activity of Nat-hyg5 generates 3-HBA from chorismate. Finally, we achieved selective production of unantimycins B and C by engineering a chassis host. On the basis of these findings, we propose that unantimycin biosynthesis is directed by the neoantimycin-producing NRPS–PKS complex and initiated with the starter unit of 3-HBA. The elucidation of the biosynthetic unantimycin pathway reported here paves the way to improve the yield of these compounds for evaluation in oncotherapeutic applications.

9 August 2019

The use of AI in counterterrorism is not inherently wrong, and this paper suggests some necessary conditions for legitimate use of AI as part of a predictive approach to counterterrorism on the part of liberal democratic states.

Neoantimycins are anticancer compounds of 15-membered ring antimycin-type depsipeptides. They are biosynthesized by a hybrid multimodular protein complex of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS), typically from the starting precursor 3-formamidosalicylate. Examining fermentation extracts of Streptomyces conglobatus, here we discovered four new neoantimycin analogs, unantimycins B–E, in which 3-formamidosalicylates are replaced by an unusual 3-hydroxybenzoate (3-HBA) moiety. Unantimycins B–E exhibited levels of anticancer activities similar to those of the chemotherapeutic drug cisplatin in human lung cancer, colorectal cancer, and melanoma cells. Notably, they mostly displayed no significant toxicity toward noncancerous cells, unlike the serious toxicities generally reported for antimycin-type natural products. Using site-directed mutagenesis and heterologous expression, we found that unantimycin productions are correlated with the activity of a chorismatase homolog, the nat-hyg5 gene, from a type I PKS gene cluster. Biochemical analysis confirmed that the catalytic activity of Nat-hyg5 generates 3-HBA from chorismate. Finally, we achieved selective production of unantimycins B and C by engineering a chassis host. On the basis of these findings, we propose that unantimycin biosynthesis is directed by the neoantimycin-producing NRPS–PKS complex and initiated with the starter unit of 3-HBA. The elucidation of the biosynthetic unantimycin pathway reported here paves the way to improve the yield of these compounds for evaluation in oncotherapeutic applications.

1 March 2007 , Number 11

In early November, the retiring head of Britain’s Security Service MI5, Dame Eliza Manningham-Buller, warned that the danger of a terror attack was ‘serious’ and ‘growing’, with as many as thirty plots underway. Traditional terrorism of the sort practised by the Irish Republican Army has given way to the possibility, if not the expectation, that groups such as Al Qaeda might make use of chemical, biological, radiological and nuclear weapons and materials in an attack in Britain. So what are the dangers?

Days before the Sept. 11, 2001, attacks, Osama bin Laden left his compound in Kandahar in Afghanistan and headed into the mountains. His driver, Salim Ahmed Hamdan, traveled with him. As U.S. and Northern Alliance forces stood poised to capture Kandahar a few months later, bin Laden told Hamdan t...

The clitoris is an organ that has been wildly misunderstood and mischaracterized for most of human history. The last three decades have dramatically changed the way that scientists think and talk about the clitoris; however, many myths and misconceptions remain.

Race horses -- Australia -- History.

Stuttgart : Enke, 1899.

Wiesbaden : J.F. Bergmann, 1897.

Gottingen : Dieterich, 1843.

Wurzburg : Stahel, 1867.

Stuttgart : Enke, 1897.

Berlin : J. Springer, 1893.

Heidelberg : C. Winter, 1891.

Zürich : Speidel, 1895.

Hannover : Hahn, 1869.

Hannover : C. Rumpler, 1866.

Jena : Druck von Bernhard Vopelius, 1899.