Items recovered include fossils, paintings, ancient coins, ceramics and jewelry

Each night, the institution will post an encore showing of an opera from its "Met Live in HD" series

The actor is one of 17 celebrities slated to participate in newly announced read-alongs of the series' first book

Airing on the aquarium's Twitch channel at 4 p.m. EST today, the two-hour session will focus on the video game's diverse insect population

Venturing into the wilderness for often weeks at a time, nature photographer Dawn LaPointe is used to social distancing

Learn hip-hop dance or do citizen science without leaving home this week, thanks to the internet's many intrepid artists and educators

Two girls run up the rounded walls of the Heydar Aliyev Center in the heart of Baku.

Rice terraces in Longji,China.

A Crabeater Seal (Lobodon carcinophaga) swimming by an iceberg in Antarctica on a snowy day.

Monuments in winter

In the hours after disaster struck Anchorage, an unexpected figure named Genie Chance came to the rescue

The Kent State shootings further widened the chasm among a citizenry divided over the Vietnam War

A trio of teenage lions are desperate to cross the shallows of the Luangwa River, in order to reunite with their pride. One problem: The river is infested with crocodiles, waiting for an easy meal.

Highway 8, otherwise known as the Dempster Highway, will be closed to all traffic at 5 p.m. on Friday, according to the government of the Northwest Territories.

Salt River First Nation in Fort Smith, N.W.T., is getting ready for a possible flood. 

The remote cabin could be the difference between life and death for travellers stuck on the territory’s long winter road. It was built to remember Stella Barnaby, who would have been 55 this Saturday.

The government of the Northwest Territories has made an exemption on border restrictions for a veterinarian from Alberta to practice in the territory, after he received public and political support.

Aubyn O'Grady, program director of the School of Visual Arts in Dawson City, seeks musical inspiration from some very interesting places.

Canadians helped liberate the Netherlands 75 years ago this week.

Public Health has recorded no new cases of COVID-19 on Saturday, said a news release from the government. 

Some farmers across the province are worried about getting their fuel in time for spring seeding. The Agricultural Producers Association of Saskatchewan says it has been fielding complaints this week about delays at the Co-op cardlock near Moose Jaw.

Provincial Police have a suspect in custody after a firearms incident Thursday morning at the hospital in the small northwestern Ontario community of Rainy River.

Parents-to-be who will have their new child delivered at the Thunder Bay Regional Health Sciences Centre have to follow a different set of guidelines than found at many other hospitals across Ontario.

It's been more than a week since there were any new or active cases of COVID-19 in the catchment area for the Northwestern Health Unit (NWHU), and staff can not focus increasing testing.

An industrial building in Thunder Bay was slightly damaged in a fire late Thursday afternoon.

Despite some pockets of severe activity, Canadians are succeeding at flattening the curve of the COVID-19 pandemic, the country’s federal health minister, Patty Hajdu, said Thursday.

The cyber security conference, exhibition and professional training are being organised in collaboration with Symantec, Secure Services (Mauritius) Limited, Harel Mallac Technologies, Orange, Oracle, Check Point, McAfee, SafeNet and CyberArk.

Safer Internet Day (SID) is an international education and awareness-raising effort spanning more than 100 countries around the globe to promote safer and more responsible use of online technology and mobile phones, especially amongst children and young people across the world.

Mauritius is among one of the very first countries where government has taken keen interest to protect children and youngsters on the Internet.  In this context, a Child Online Safety Action Plan was developed by the National Computer Board (NCB) and endorsed by the cabinet in 2009.  Following the aproval of the action plan, number of activities have been started on Child Online Safety since 2009 including organisation of the Safer Internet Day. 

This year is the 6th edition of Safer Internet Day which will be celebrated in Mauritius. On this occasion, CERT-MU, a division of the National Computer Board is organising several activities.  The activities are as follows:

• A half day workshop with theme "Let's create a better Internet together" in collaboration with the Ministry of Technology, Communication and Innovation and the Ministry of Education and Human Resources, Tertiary Education and Scientific Research.  The proposed workshop is targeted towards State and Private secondary school students to sensitise them on the impact of threats and consequences that improper use of the Internet may have on the youth.

• Awareness sessions on “Internet Safety” will be carried out for secondary school students (State and Private) in all four school zones of the country.  These sessions will highlight issues like sexting, sextortion, cyber bullying, online predators and identity theft etc. 

• Organisation of awareness sessions on “Dangers of the Internet” for secondary school teachers (State and Private) in all four school zones during the school holidays. Trainings on the Dangers of the Internet and how to help youngsters choose a better way of socialising in the virtual world will be organised for the teachers of  secondary schools.

• Awareness sessions for Parents, Senior Citizens and House Wives on "Secure Usage of Internet".  Parents, Grandparents and House Wives need to be aware of the dangers on the Internet and how to protect their kids online.  An awareness session will be organised to address this issue with parents, in collaboration with the PTA of secondary schools.  The Senior Citizens and House Wives sessions will take place in NCB cyber caravans and community welfare centres. 

• Dissemination of brochures on "Child Online Protection" to the students and teachers.

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A man is in police custody following an incident that briefly put the Burin Peninsula community of Lawn on high alert on Friday morning. 

Long-term care homes in Eastern Health's jurisdiction are allowing for people to again visit their relatives by windows, just in time for Mother's Day. 

From age 17, Ana Maria prayed to serve God in Switzerland. While she waited, she became a dance instructor with no idea dance would become her ministry.

Este año en el mes en que se llevó a cabo el mantenimiento anual de Logos Hope en Uruguay, la tripulante Cecilia* de Argentina se unió a un pequeño equipo que sirve en Asia Central. Mientras estuvo allí, pudo asistir a dos iglesias subterráneas que desbordaban de esperanza y fe.

Three residents at a long-term care facility in Sault Ste. Marie have tested positive for COVID-19, prompting officials to declare an outbreak at Extendicare Maple View.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

The Porcupine Health Unit is declaring a COVID-19 outbreak at a long-term care home in Iroquois Falls.

A former miner who was forced to breathe in a fine aluminum dust before each shift says he’s not surprised a new report links that dust and a higher risk of Parkinson’s disease.

The City of Timmins says a COVID-19 outbreak remains in place at a long-term care home in the city, even after the one affected resident has now tested negative — twice.

There are now 176 confirmed cases of COVID-19 in northeastern Ontario, and seven deaths.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

Mice, rats, or hamsters are immunized by giving biweekly injections of a purified antigen, cultured cells, or cDNA. For mice, if a pure, soluble protein antigen is being used and is abundant, a dose of 50–100 µg in adjuvant at each immunization is a sensible general recommendation; for rats and hamsters, a dose of 100–200 µg is sufficient. Lower doses can be used for antigens with higher immunogenicity. Adjuvants (Freund's, Ribi, Hunter's TiterMax, ImmunEasy, or Alum) should be mixed with the immunizing antigen for the first two immunizations only; Complete Freund's adjuvant is only used with the first immunization. Subsequent immunizations are performed in phosphate-buffered saline (PBS) or normal saline, with or without Incomplete Freund's adjuvant. The choice of adjuvant is dependent on the subclass of immunoglobulin required. Over the course of the 6-wk immunization schedule, each animal usually receives a total of six injections (three subcutaneous and three intraperitoneal). Once a good titer has developed against the antigen of interest, regular boosts and bleeds are performed to collect the maximum amount of serum. For rats and hamsters, boosts should be spaced every 2–3 wk, and serum samples of 400–500 µL should be collected 10–12 d after each boost. For mice, boosts should be spaced every 2–3 wk, and serum samples of 200–300 µL should be collected 10–12 d after each boost.

The Bradford assay is a quick and fairly sensitive method for measuring the concentrations of proteins. It is based on the shift in absorbance maximum of Coomassie Brilliant Blue G-250 dye from 465 to 595 nm following binding to denatured proteins in solution.

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.