tia Indiana receives more than 57K new initial claims for unemployment insurance benefits By rssfeeds.indystar.com Published On :: Thu, 30 Apr 2020 15:20:20 +0000 New data from the U.S. Department of Labor reflects the continued toll of the coronavirus on Indiana workers. Full Article
tia Athlete spotlight: Catching up with Greenwood Christian senior Champ McCorkle By rssfeeds.indystar.com Published On :: Thu, 16 Jan 2020 18:01:28 +0000 McCorkle leads the Class A second-ranked Cougars in scoring (13.7 ppg), rebounding (6.9) and assists (4.2) through 11 games this season. Full Article
tia Greenwood Christian wins first Johnson County title, likely will get top spot in Class A By rssfeeds.indystar.com Published On :: Sun, 19 Jan 2020 02:58:30 +0000 Champ McCorkle notched his 1,000th-career point as the Cougars beat Center Grove for the Johnson County tournament championship. Full Article
tia Greenwood Christian's star has a 'motor that doesn't stop.' Her numbers are mind-boggling. By rssfeeds.indystar.com Published On :: Tue, 28 Jan 2020 16:11:44 +0000 Meet Isabella Reed, the 5-9 Greenwood Christian Academy junior and superstar who doesn't know it. Or at least won't admit it. Full Article
tia Greenwood Christian AD Sue Tameling dies from apparent heart attack: 'She very much loved GCA.' By rssfeeds.indystar.com Published On :: Tue, 07 Apr 2020 17:06:57 +0000 Sue Tameling was in her role as Greenwood Christian AD for less than two years, but she left an impression as a mentor, teacher and a friend. Full Article
tia Girls Top 60 senior workout on Sunday at Heritage Christian By rssfeeds.indystar.com Published On :: Sun, 08 Mar 2020 14:22:46 +0000 Two sessions on Sunday at Heritage Christian Full Article
tia The Presidential Pooch 总统家的小狗 By www.bbc.co.uk Published On :: 2009-04-23T14:08:48+00:00 There's a new face grabbing the headlines in the White House in Washington, and it's not Barack Obama. Full Article Tae Takeaway English
tia Coronavirus: What county cricket has to do to potentially return this summer By www.bbc.co.uk Published On :: Thu, 07 May 2020 11:11:33 GMT Northamptonshire club doctor Professor Bill Ribbans tells BBC Look East what cricket must do in order to return this summer. Full Article
tia Project Restart: Your questions answered on sport's potential return By www.bbc.co.uk Published On :: Sat, 09 May 2020 09:27:05 GMT In a BBC Sport special, we answer your questions on the potential return of sport amid the coronavirus pandemic. Full Article
tia Letters: Essential workers deserve respect — even after coronavirus crisis ends By rssfeeds.indystar.com Published On :: Thu, 07 May 2020 08:30:08 +0000 I see people praising restaurant workers, grocery clerks, and truck drivers and I think this is long overdue. Full Article
tia Does pandemic offer US and Iran chance for partial reset? By www.bbc.co.uk Published On :: Thu, 19 Mar 2020 02:15:00 GMT Jonathan Marcus looks at whether the crisis might lead to some rapprochement between the arch-foes. Full Article
tia Qatar Airways warns of 'substantial' job losses By www.bbc.co.uk Published On :: Wed, 06 May 2020 06:46:25 GMT State-owned Qatar Airways plans significant staff cutbacks as it deals with the coronavirus downturn. Full Article
tia Croatia timeline By news.bbc.co.uk Published On :: Mon, 23 Jan 2012 10:15:28 GMT A chronology of key events Full Article Country profiles
tia Regions and territories: South Ossetia By news.bbc.co.uk Published On :: Wed, 25 Apr 2012 09:41:55 GMT An overview of South Ossetia, including key facts and political leaders Full Article Country profiles
tia Regions and territories: North Ossetia By news.bbc.co.uk Published On :: Tue, 29 Nov 2011 10:08:52 GMT Key facts, dates and events Full Article Country profiles
tia Regions and territories: Ingushetia By news.bbc.co.uk Published On :: Tue, 22 Nov 2011 14:53:08 GMT Key facts, figures and dates Full Article Country profiles
tia Croatia country profile By news.bbc.co.uk Published On :: Mon, 23 Jan 2012 10:00:04 GMT Key facts, figures and dates Full Article Country profiles
tia ‘Bloodshot’ works, if only for the cinematic universe it has the potential to create By www.washingtonpost.com Published On :: Thu, 12 Mar 2020 15:41:12 +0000 Vin Diesel stars as Bloodshot as Valiant Comics finally makes it to the big screen. Full Article
tia Walmart has added virtual reality to its assessment of an employee’s potential By www.washingtonpost.com Published On :: Fri, 12 Jul 2019 20:35:18 +0000 For the past few months, the nation’s largest employer has begun evaluating workers using an experimental tool: virtual reality. Full Article
tia In Silicon Valley, some men say cosmetic procedures are essential to a career By www.washingtonpost.com Published On :: Thu, 09 Jan 2020 15:00:26 +0000 Women have long felt the pressure of looking the part. Now men are feeling it, too. Full Article
tia Week 14 NFL betting tips: The Cowboys' potential surges while the Packers’ fades By www.washingtonpost.com Published On :: Tue, 04 Dec 2018 16:14:33 +0000 Meanwhile there's not much value left in division title futures, with the Los Angeles Rams (-15000), New Orleans Saints (-3300) and New England Patriots (-5000) are all huge favorites to win their respective divisions. Full Article
tia Presidential hopefuls swap the campaign trail for the field at Congressional Women’s Softball Game By www.washingtonpost.com Published On :: Thu, 20 Jun 2019 20:25:45 +0000 The Members team ultimately fell 10-4 to the press team, despite valiant efforts from players such as 2020 presidential hopeful and team pitcher Kirsten Gillibrand. Full Article
tia Michelle Obama talks about her last night at the White House, Democratic presidential candidates in interview By www.washingtonpost.com Published On :: Sun, 07 Jul 2019 19:23:59 +0000 “The transition happens so quickly; it’s like you don’t even get to move your furniture out until the new president takes the oath of office,” Obama told Gayle King in an interview at Essence Festival. Full Article
tia strataconf: Read our initial findings of @amplab's open source benchmark for tracking large-scale Query Engines http://t.co/rGgOVeTHQ8 #strataconf By twitter.com Published On :: Fri, 07 Jun 2013 21:16:23 +0000 strataconf: Read our initial findings of @amplab's open source benchmark for tracking large-scale Query Engines http://t.co/rGgOVeTHQ8 #strataconf Full Article
tia 4 High-Potential Sectors for AI and ML Startup Success By feedproxy.google.com Published On :: Wed, 01 Jan 2020 19:00:21 +0000 Today, the explosion of development in artificial intelligence (AI) and machine learning (ML) technology has created a market for which it appears there’s no limit. No matter the industry, if you name a reasonably-sized (or larger) company, there’s a good chance that they’re investing in AI and ML technology as a cornerstone of their strategic […] The post 4 High-Potential Sectors for AI and ML Startup Success appeared first on ReadWrite. Full Article AI Startups artificial intelligence HR Human Resources Machine Learning ML startups
tia 7 Common Negotiation Mistakes to Avoid at all Costs By feedproxy.google.com Published On :: Thu, 02 Jan 2020 16:00:27 +0000 Many often consider negotiation an arduous task, as it involves having conversations with people, where both parties are doing their best to protect personal interests. Going over negotiations while maintaining relationships remains relevant to businesses and investments. It hardly comes easy to conclude a negotiation that keeps both parties happy, while overlooking small processes may […] The post 7 Common Negotiation Mistakes to Avoid at all Costs appeared first on ReadWrite. Full Article Entrepreneurs Marketing Negotiation Negotiation Agreement negotiation process
tia Less than essential: Why smart speakers are like smartwatches, not smartphones By feeds.searchengineland.com Published On :: Wed, 06 May 2020 08:40:25 +0000 The devices have reached 50% of US homes, but fallen flat as a channel Please visit Search Engine Land for the full article. Full Article
tia AT#133 - Travel the Croatia Backroads By traffic.libsyn.com Published On :: Sun, 20 Apr 2008 01:10:00 +0000 Croatia Backroads Full Article
tia AT#260 - Biking Spain's Camino di Santiago By europe.amateurtraveler.com Published On :: Sat, 25 Dec 2010 15:00:00 +0000 The Amateur Traveler talks to Ben Dixon about biking the route of the Camino di Santiago in northern Spain. The Camino di Santiago is a an ancient pilgrim trail that runs across northern Spain from the border with France to Santiago di Compostella. The cathedral in Santiago di Compostella reputedly holds the remains of St. James. Modern day pilgrims are sometimes drawn by faith and sometimes just drawn by the journey itself to trek for a month across the varied landscapes of northern Spain. As an American, Ben did not have a month of vacation but rather than be one of those pilgrims who only traverses the very end of the trail he chose to take on the pilgrim route on a bicycle. Along the way he stayed in the hostels that house the many pilgrims where he met people who had been walking from far beyond the border with France. Through countryside, cities and picturesque towns he rode in both sunshine and driving rain, but the highlight of the trip might not be what you think. Full Article
tia AT#414 - Travel to Nova Scotia By canada.amateurtraveler.com Published On :: Sun, 06 Apr 2014 04:42:47 +0000 Hear about travel to Nova Scotia as the Amateur Traveler talks to Sherry Ott about her recent trip to this province in Eastern Canada. Sherry returns to to the show to talk about a trip around Nova Scotia she took last June. Full Article
tia AT#482 - Hiking the Camino de Santiago in Spain By europe.amateurtraveler.com Published On :: Sat, 05 Sep 2015 15:14:47 +0000 Hear about hiking the Camino de Santiago (the Way of St. James) in northern Spain as the Amateur Traveler talks to Linda Martin from The Indie Travel Podcast. Linda says, "the thing about the Camino is that it is as long as you wanted to be." Full Article
tia AT#489 - Travel to Croatia By europe.amateurtraveler.com Published On :: Sat, 07 Nov 2015 16:30:00 +0000 Hear about travel to Croatia as the Amateur Traveler talks to Jay Ternavan of JaywayTravel.com about this country on the coast of the Adriatic. Jay says, "for me Croatia has everything you could look for in a travel experience" Full Article
tia Mechanistic insights explain the transforming potential of the T507K substitution in the protein-tyrosine phosphatase SHP2 [Signal Transduction] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 The protein-tyrosine phosphatase SHP2 is an allosteric enzyme critical for cellular events downstream of growth factor receptors. Mutations in the SHP2 gene have been linked to many different types of human diseases, including developmental disorders, leukemia, and solid tumors. Unlike most SHP2-activating mutations, the T507K substitution in SHP2 is unique in that it exhibits oncogenic Ras-like transforming activity. However, the biochemical basis of how the SHP2/T507K variant elicits transformation remains unclear. By combining kinetic and biophysical methods, X-ray crystallography, and molecular modeling, as well as using cell biology approaches, here we uncovered that the T507K substitution alters both SHP2 substrate specificity and its allosteric regulatory mechanism. We found that although SHP2/T507K exists in the closed, autoinhibited conformation similar to the WT enzyme, the interactions between its N-SH2 and protein-tyrosine phosphatase domains are weakened such that SHP2/T507K possesses a higher affinity for the scaffolding protein Grb2-associated binding protein 1 (Gab1). We also discovered that the T507K substitution alters the structure of the SHP2 active site, resulting in a change in SHP2 substrate preference for Sprouty1, a known negative regulator of Ras signaling and a potential tumor suppressor. Our results suggest that SHP2/T507K's shift in substrate specificity coupled with its preferential association of SHP2/T507K with Gab1 enable the mutant SHP2 to more efficiently dephosphorylate Sprouty1 at pTyr-53. This dephosphorylation hyperactivates Ras signaling, which is likely responsible for SHP2/T507K's Ras-like transforming activity. Full Article
tia Non-photopic and photopic visual cycles differentially regulate immediate, early, and late phases of cone photoreceptor-mediated vision [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 Cone photoreceptors in the retina enable vision over a wide range of light intensities. However, the processes enabling cone vision in bright light (i.e. photopic vision) are not adequately understood. Chromophore regeneration of cone photopigments may require the retinal pigment epithelium (RPE) and/or retinal Müller glia. In the RPE, isomerization of all-trans-retinyl esters to 11-cis-retinol is mediated by the retinoid isomerohydrolase Rpe65. A putative alternative retinoid isomerase, dihydroceramide desaturase-1 (DES1), is expressed in RPE and Müller cells. The retinol-isomerase activities of Rpe65 and Des1 are inhibited by emixustat and fenretinide, respectively. Here, we tested the effects of these visual cycle inhibitors on immediate, early, and late phases of cone photopic vision. In zebrafish larvae raised under cyclic light conditions, fenretinide impaired late cone photopic vision, while the emixustat-treated zebrafish unexpectedly had normal vision. In contrast, emixustat-treated larvae raised under extensive dark-adaptation displayed significantly attenuated immediate photopic vision concomitant with significantly reduced 11-cis-retinaldehyde (11cRAL). Following 30 min of light, early photopic vision was recovered, despite 11cRAL levels remaining significantly reduced. Defects in immediate cone photopic vision were rescued in emixustat- or fenretinide-treated larvae following exogenous 9-cis-retinaldehyde supplementation. Genetic knockout of Des1 (degs1) or retinaldehyde-binding protein 1b (rlbp1b) did not eliminate photopic vision in zebrafish. Our findings define molecular and temporal requirements of the nonphotopic or photopic visual cycles for mediating vision in bright light. Full Article
tia Brain manganese and the balance between essential roles and neurotoxicity [Molecular Bases of Disease] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 Manganese (Mn) is an essential micronutrient required for the normal development of many organs, including the brain. Although its roles as a cofactor in several enzymes and in maintaining optimal physiology are well-known, the overall biological functions of Mn are rather poorly understood. Alterations in body Mn status are associated with altered neuronal physiology and cognition in humans, and either overexposure or (more rarely) insufficiency can cause neurological dysfunction. The resultant balancing act can be viewed as a hormetic U-shaped relationship for biological Mn status and optimal brain health, with changes in the brain leading to physiological effects throughout the body and vice versa. This review discusses Mn homeostasis, biomarkers, molecular mechanisms of cellular transport, and neuropathological changes associated with disruptions of Mn homeostasis, especially in its excess, and identifies gaps in our understanding of the molecular and biochemical mechanisms underlying Mn homeostasis and neurotoxicity. Full Article
tia 5-Ethynyl-2'-deoxycytidine and 5-ethynyl-2'-deoxyuridine are differentially incorporated in cells infected with HSV-1, HCMV, and KSHV viruses [Microbiology] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 Nucleoside analogues are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine analogues 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyl-2'-deoxycytidine (EdC). Click chemistry enables conjugation of an azide molecule tagged with a fluorescent dye or biotin to the alkyne of the analog, which can then be used to detect incorporation of EdU and EdC into DNA. The use of EdC is often recommended because of the potential cytotoxicity associated with EdU during longer incubations. Here, by comparing the relative incorporation efficiencies of EdU and EdC during short 30-min pulses, we demonstrate significantly lower incorporation of EdC than of EdU in noninfected human fibroblast cells or in cells infected with either human cytomegalovirus or Kaposi's sarcoma-associated herpesvirus. Interestingly, cells infected with herpes simplex virus type-1 (HSV-1) incorporated EdC and EdU at similar levels during short pulses. Of note, exogenous expression of HSV-1 thymidine kinase increased the incorporation efficiency of EdC. These results highlight the limitations when using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recovery and sensitivity for downstream applications. This is an important discovery that may help to better characterize the biochemical properties of different nucleoside analogues with a given kinase, ultimately leading to significant differences in labeling efficiency of nascent DNA. Full Article
tia By enabling formal trade, Nigeria can unleash its vast potential By feedproxy.google.com Published On :: Mon, 07 Dec 2015 16:18:19 +0000 3 December 2015 20151207Nigeriabooming.jpg Nigeria’s booming informal trade is costly for society, business and government, yet a critical opportunity exists to formalize such trade and drive more sustainable and less volatile growth, argues a new report from Chatham House.According to one estimate, informal activity accounts for up to 64 per cent of Nigeria’s GDP. Nigeria's Booming Borders: The Drivers and Consequences of Unrecorded Trade finds that this is a result of obstacles that impede trading through formal channels. These drivers include bureaucratic burdens and other factors, such as:The need for Nigerian businesses to produce at least nine documents in order to send an export shipment and at least 13 in order to bring in an import consignment.Rigid and dysfunctional foreign-exchange regulations that push most smaller traders into the incompletely regulated parallel exchange market.Corruption and unofficial ‘taxation’, especially on major border highways, which delegitimize formal channels and encourage the use of smuggling routes.As a result, the state loses direct tax revenues that would be generated by formal cross-border trade. This is not just siphoned into the informal economy; some is lost entirely. For example, many shippers opt to dock in neighbouring countries rather than deal with the expense and difficulty of using Nigeria’s ports.Informal trade also undermines the social contract between the private sector and government. The state lacks tax revenues to pay its officials, improve infrastructure or implement reforms, while traders feel the government provides no services in return for any taxes they might pay.‘Every day tens of thousands of unofficial payments are made, none destined for the government. Policy-makers need to create an environment that encourages trade to flow through formal channels and capture lost revenue’, says co-author Leena Koni Hoffmann.‘Formalization would assist Nigeria to pursue more high-quality, high-tech economic activity at a time when rising labour costs in Asia are creating scope for Nigerian manufacturers to compete’, she adds.The report makes a number of recommendations for how Nigeria could encourage more formal trade, including:Strengthening the resources and capacity of the Federal Ministry of Industry, Trade and Investment to coordinate action across key government ministries, departments and agencies, as well as public and private stakeholders.Prioritizing engagement in the development of Economic Community of West African States (ECOWAS) trade policies and fully implementing the ECOWAS Protocol on Free Movement of Persons to reduce harassment at borders.Allowing banks to operate simple services for small and medium-sized businesses to make trade payments directly from Nigerian naira to CFA francs and vice versa.Improving basic facilities that support traders, including improving the efficiency of border posts, installing truck parks and all-weather surfacing on market access roads, and introducing online booking for trucks to enter ports.Separating responsibilities for assessing duty and tariff liabilities from revenue collection in order to reduce opportunities for corruption, an approach already tested with success by the Lagos State Internal Revenue Service.Increasing funding and technical support for the National Bureau of Statistics, which has a significant role to play in measuring and capturing more of Nigeria’s external trade.Interviews conducted for the report reveal that business people would welcome the opportunity to pay taxes, but only if they received assurance that these payments would represent a contract with government guaranteeing that conditions for business would be improved.‘As Africa’s largest economy, formalizing external trade would allow Nigeria to fulfil its potential as the trading engine of the West and Central African economy and shape the business landscape across the region,’ says co-author Paul Melly. Editor's notes Read Nigeria's Booming Borders: the Drivers and Consequences of Unrecorded Trade (embargoed until 17:00 GMT on Monday 7 December).To request an interview with the authors, contact the press office.Nigeria’s recorded external trade for 2014 was $135.8 billion.Estimate of informal activity as a percentage of GDP from Jonathan Emenike Ogbuabor and Victor A. Malaolu, ‘Size and Causes of the Informal Sector of the Nigerian Economy: Evidence from Error Correction Mimic Model’, Journal of Economics and Sustainable Development, Vol. 4, No. 1, 2013. Contacts Press Office +44 (0)20 7957 5739 Email Full Article
tia Moving Energy Initiative Starts Clean Energy Projects for Refugees By feedproxy.google.com Published On :: Mon, 26 Jun 2017 12:25:21 +0000 26 June 2017 The Moving Energy Initiative starts four new clean energy projects for refugees. Combo_large_LCP (2).jpg Refugees in Burkina Faso, Kenya and Jordan will benefit from greater access to affordable, clean energy for domestic use and to power ‘microbusinesses’, following a grant scheme from the Moving Energy Initiative which launches today.The Moving Energy Initiative is supporting projects ranging from a solar-powered ICT hub in Kenya’s Kakuma camp and vegetable growing zones watered by solar-powered pumps in Burkina Faso’s Goudoubo camp, to reliable energy generation for north Jordan’s Al Mafraq hospital where Syrian refuges and local residents access health care.Embed this image <img src="https://www.chathamhouse.org/sites/default/files/images/Combo_large_LCP.jpg" alt="" title="" />In total, four projects will be implemented over the next 12 months, with all involving training for refugees and local staff to use and maintain the clean energy technologies. They aim to deliver tangible reductions in CO2 emissions whilst increasing access to vital services, saving costs and providing livelihood opportunities for local communities and refugees. The projects are also expected to fuel entrepreneurship, opening up the potential for setting up businesses, small shops and restaurants which can expand beyond this initial grant. Project partners were chosen through an open and competitive process kicked off in February 2017 in Burkina Faso, Kenya and Jordan. Bidders were asked to demonstrate their ability to provide solutions that reduce fossil fuel consumption and increase access to energy in camps. The successful projects were chosen on the basis of the applicants’ ability to innovate in a humanitarian setting, as well as their track record and their approach to sustainability.Find out more about why these projects are needed and the impact they aim to have by watching the video: Check out the Moving Energy Initiative website for more information.Keep updated on the progress of these projects as they develop by following us on Twitter @CH_EERD. Related contentMoving Energy Initiative: Sustainable Energy for Refugees and Displaced PeopleThe Moving Energy Initiative Expert WorkshopToolkits for the Moving Energy Initiative Full Article
tia Brexit Clouds TTIP Negotiations But May Not Scupper Deal By feedproxy.google.com Published On :: Fri, 08 Jul 2016 09:12:24 +0000 11 July 2016 Marianne Schneider-Petsinger Senior Research Fellow, US and the Americas Programme @mpetsinger The British vote to leave the EU will slow progress on a transatlantic trade deal, but it also removes some UK sticking points from the process. 2016-07-08-TTIP.jpg A sign promoting the TTIP free trade agreement in Berlin. Photo by Getty Images. With Britain’s decision to leave the EU, the clouds of uncertainty hanging over the proposed US-EU free trade deal (known as the Transatlantic Trade and Investment Partnership or TTIP) have become darker. The negotiations were formally launched three years ago and have stalled because of transatlantic differences (for instance over issues of investor protections and public procurement) as well as growing public opposition. For now, both the US and the EU negotiators are determined to weather the storm and continue talks when they meet in Brussels from 11-15 July.The result of the UK’s EU referendum will blow a strong wind into the face of TTIP negotiators on three fronts. First, the Brexit vote will delay the TTIP talks as EU officials will focus their attention and political capital on the future UK-EU relationship. Once the UK government triggers Article 50 of the Lisbon Treaty, both sides have two years to sort out the separation proceedings. Only after it has become clear what Britain’s relationship with the EU will look like will the European side stop navel-gazing. The TTIP negotiations will likely continue in the meantime, but will be put on the back-burner.Second, any progress on TTIP will require clarity on what both sides are bringing to the negotiating table. But until the final nature of the UK-EU relationship is known, it will be difficult for the American side to assess exactly how valuable the access to the remaining EU market is. This raises the question of whether American negotiators will put forth their best offers if they don’t know what benefits they will obtain for making concessions.Third, with Britain’s vote to leave the EU, TTIP has just lost one of its greatest cheerleaders. French and German officials are increasingly expressing concerns about TTIP. Within three days of the Brexit vote, French Prime Minister Manuel Valls dismissed the possibility of a US-EU trade deal, stating TTIP was against ‘EU interests’. In addition, 59 per cent of Germans oppose TTIP – up from 51 per cent – according to the most recent Eurobarometer survey. Britain’s voice for further trade liberalization will be sorely missed by American negotiators eager to strike a deal.Despite the dark Brexit clouds on the TTIP horizon, there might be a silver lining. Britain’s decision to leave the EU could bring some benefits to the US-EU trade talks in two ways. First, financial services regulation might no longer be a sticking point in the TTIP negotiations. Given London’s role as a financial centre, the UK had insisted on including a financial services chapter in the trade deal. The US, however, has resisted this. The removal of this friction could help move the TTIP negotiations along.Second, European trade negotiators will no longer have to address British fears that TTIP could put the National Health Service (NHS) at risk. Much of the TTIP-debate in Great Britain has focused on how this deal might impact the NHS. Opponents of TTIP have argued that including healthcare in the agreement could lead to privatization and ultimately the death of the NHS. EU Trade Commissioner Cecilia Malmström spent resources and energy in correcting these misconceptions. UK withdrawal from the EU means that she can now focus on fighting other myths surrounding TTIP, which could potentially help advance the trade deal.For now and the immediate future, Britain will remain a member of the EU and the European Commission will continue to negotiate trade deals on behalf of all 28 member states. Both the US and EU negotiators are committed to advancing the trade deal despite Brexit. The British decision to leave the EU has not weakened the case for TTIP. Speaking on the outcome of the EU referendum, United States Trade Representative Michael Froman said ‘the economic and strategic rationale for TTIP remains strong’. And his counterpart Cecilia Malmström went even further, saying that the British decision to leave the EU creates more of an impetus for TTIP to be finished this year.Though this timeline is unlikely to be met, TTIP is likely to survive the British decision to leave the EU. However, Brexit is a serious blow that will probably push back the conclusion of TTIP by at least two years. Any deal will need to take into account the future nature of the UK-EU trade deal, which may not be known before 2018. Meanwhile, elections in Germany and France (two countries with strong public opposition to TTIP) will take place in 2017. On the other side of the Atlantic, the US presidential election adds yet another layer of uncertainty as the trade policy of the next administration remains unknown. When US and EU trade negotiators meet again this week, they should not be too worried about the Brexit storm but rather the changing climate for TTIP in France, Germany and the US.To comment on this article, please contact Chatham House Feedback Full Article
tia Realizing TTIP’s Strategic Potential By feedproxy.google.com Published On :: Tue, 12 Jul 2016 15:34:44 +0000 14 July 2016 The strategic case for TTIP is greater – and the stakes higher – now that the UK has decided to leave the EU. But TTIP will create new risks for the West whether it succeeds or fails. Download PDF Gregor Irwin Chief economist, Global Counsel 2016-07-14-ttip.jpg The container ship Osaka Express, operated by Hapag-Lloyd AG, leaves the container terminal at the port in Southampton, UK, on 2 October 2015. Photo: Getty Images. SummaryThe Transatlantic Trade and Investment Partnership (TTIP) – currently being negotiated between the United States and the European Union – represents a bolder and riskier approach to liberalizing trade than traditional trade deals. It is bolder because it aims to cover a wide range of policy issues that are not typically included, and because it aims to be strategic and extraterritorial in its impact. It is riskier because of the difficulty in getting agreement on these issues between the parties concerned, and because the responses of other countries are uncertain.EU and US negotiators have much work to do if a deal is to be reached that realizes TTIP’s full potential. While progress has been made on tariff reduction, considerable ground still needs to be covered on setting standards and regulations affecting trade, which is where TTIP has the most potential to make an impact at a strategic level. Meanwhile, trade scepticism is rising on both sides of the Atlantic, with concerns about the impact of deals like TTIP on jobs and regulatory standards. The negotiation process is also now complicated by the United Kingdom’s decision to leave the EU. Making a clear and credible strategic case for TTIP may be necessary if the negotiations are to continue and be successful.If the United States and the EU are able to agree on the regulations and standards affecting international trade, they will have the scale to define these globally for years to come. International leadership in this area brings commercial advantages and benefits for consumers in the EU and the United States, and it also often is a global public good. However, it sometimes pits EU and US interests against those of other countries, creating scope for conflict over policies in areas such as the rules governing state-owned enterprises.There would be benefits to the United Kingdom, the EU and the United States if the United Kingdom joins TTIP once it is agreed. In the intervening period, it makes sense for the United Kingdom to participate actively in EU decisions regarding TTIP while still a member of the EU, as this could help to smooth an eventual British accession to the partnership.The soft-power benefits from TTIP are potentially substantial, but they would only be maximized if other strategically important countries, such as Turkey, are able to join. Perhaps the clearest sign of TTIP being likely to meet or exceed the highest ambitions for its strategic impact would be if it has an even broader geographical reach, drawing in countries in other regions.The potential security benefits from TTIP are marginal and overhyped. The benefits that unity on trade would bring to transatlantic security are intangible and hard to substantiate. The energy security benefits are likely to be limited, as the volumes of US liquefied natural gas (LNG) exports to the EU are likely to be small. If the EU and the United States are serious about using TTIP to improve security, they should include defence procurement, but this has never been on the agenda.Not only do the differences between the EU and the United States mean that it will be difficult to get a deal, they also constrain how any deal would be implemented and exploited for strategic purposes. If TTIP is to succeed at a strategic level, both sides must be disciplined, consistent and coordinated in using it as the reference point in their negotiations with other countries. The United States is more capable of acting strategically than the EU, in part because of the difficulties for EU member states in coalescing around a shared set of strategic objectives. Until the EU is able to do this, the United States is likely to have much more influence over the strategic direction of TTIP. If the EU wants to bridge this gap, then the European Council should start by reaching a political agreement on its objectives and priorities for bringing other countries into TTIP.There are also strategic risks from TTIP. One is that by emphasizing the values that are reflected in international rules, the EU and the United States could make it harder for other countries to accept these rules, or make TTIP seem like an attempt to reassert the old world order. Thus, instead of having a magnetic effect, TTIP could create a rift with emerging countries, with some choosing to maintain a distance for political reasons.The bigger risk for TTIP, however, is failure. If negotiations break down or a deal is reached that falls short of the ambition set for it, this would send a damaging signal to the rest of the world about the inability of the EU and the United States to work together. The damage would be all the greater if the process was acrimonious, or if it exposed US indifference to Europe or latent anti-Americanism in the EU. The United Kingdom’s decision to leave the EU raises the stakes, particularly for the latter, as it is a blow to the international credibility of the EU. Agreeing TTIP would help to offset this blow, while failure would amplify it. Department/project US and the Americas Programme, US Geoeconomic Trends and Challenges Full Article
tia RNA helicase-regulated processing of the Synechocystis rimO-crhR operon results in differential cistron expression and accumulation of two sRNAs [Gene Regulation] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts. Full Article
tia Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein By feedproxy.google.com Published On :: 2005-09-01 Yasushi IshihamaSep 1, 2005; 4:1265-1272Research Full Article
tia Mechanistic insights explain the transforming potential of the T507K substitution in the protein-tyrosine phosphatase SHP2 [Signal Transduction] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 The protein-tyrosine phosphatase SHP2 is an allosteric enzyme critical for cellular events downstream of growth factor receptors. Mutations in the SHP2 gene have been linked to many different types of human diseases, including developmental disorders, leukemia, and solid tumors. Unlike most SHP2-activating mutations, the T507K substitution in SHP2 is unique in that it exhibits oncogenic Ras-like transforming activity. However, the biochemical basis of how the SHP2/T507K variant elicits transformation remains unclear. By combining kinetic and biophysical methods, X-ray crystallography, and molecular modeling, as well as using cell biology approaches, here we uncovered that the T507K substitution alters both SHP2 substrate specificity and its allosteric regulatory mechanism. We found that although SHP2/T507K exists in the closed, autoinhibited conformation similar to the WT enzyme, the interactions between its N-SH2 and protein-tyrosine phosphatase domains are weakened such that SHP2/T507K possesses a higher affinity for the scaffolding protein Grb2-associated binding protein 1 (Gab1). We also discovered that the T507K substitution alters the structure of the SHP2 active site, resulting in a change in SHP2 substrate preference for Sprouty1, a known negative regulator of Ras signaling and a potential tumor suppressor. Our results suggest that SHP2/T507K's shift in substrate specificity coupled with its preferential association of SHP2/T507K with Gab1 enable the mutant SHP2 to more efficiently dephosphorylate Sprouty1 at pTyr-53. This dephosphorylation hyperactivates Ras signaling, which is likely responsible for SHP2/T507K's Ras-like transforming activity. Full Article
tia In Next Round of EU Negotiations, Britain Faces Familiar Pitfalls By feedproxy.google.com Published On :: Fri, 31 Jan 2020 14:33:27 +0000 31 January 2020 Thomas Raines Director, Europe Programme @TomHRaines Google Scholar Professor Richard G Whitman Associate Fellow, Europe Programme @RGWhitman Google Scholar Despite being free of the constraints and the theatre of a hung parliament, there is a risk that over the coming year the British government repeats too many of the mistakes of the withdrawal negotiations. 2020-01-31-Ldn.jpg The Elizabeth Tower remains under renovation on 31 January 2020. Photo: Getty Images. Whether feared or longed for, Brexit day has arrived. It is positive for all sides that the process is thus far managed and ordered, with debts paid, rights guaranteed and borders still invisible on the island of Ireland. But in a difficult new phase of negotiations, as the UK and EU try to hammer out the terms of their relationship after 2020, Britain is at risk of repeating many of its mistakes from the withdrawal talks.First, the government, through the negotiation timeline, has reduced its own room for manoeuvre. The failure of the initial withdrawal agreement and subsequent turbulent politics have reduced a planned 21-month transition to an 11-month one. Even though half the original negotiation time has been lost, 31 December 2020 remains in place and indeed has been written into UK law as the date the transition arrangement ends. Boris Johnson has followed Theresa May in investing symbolism and significance in an arbitrary date.By promising not to extend negotiations, the UK is boxing itself in, creating domestic political barriers where it may well need flexibility. The familiar face of Michel Barnier, who proved adept in leading the withdrawal negotiations for the EU side, will be back in phase two to tell Britain the clock is ticking. This new timeline is intended to focus minds but more likely it will limit ambitions. Second, this government has continued the pattern of its predecessor in making no effort to manage public expectations about the consequences of Brexit. It is naïve to have followed the last years of British politics and expect an outbreak of sobriety and levelheadedness. The entrenched positions of each side have offered little political space or electoral incentive for realism.During the 2020 transition period, the UK will lose the political rights of EU membership but it will retain the benefits and obligations. Most citizens and business will not be able to tell the difference. But a reckoning is inevitable. There will come a moment when the effects of this slow-motion political revolution – particularly in the hard form envisioned by Boris Johnson – become real, when the trade-offs and compromises, especially for business and the economy, will bite. The public deserve some realism about the price of sovereignty.Third, there is a risk that government remains underprepared. While its headline goals are clear – at least in terms of what it does not want – the UK government will need thorough, realistic and coherent proposals on what it wants in every area of negotiations, and crucially develop a process by which to make political trade-offs between the demands of different sectors and issues. The government must also then prepare for their implementation in every area. This would be a huge challenge even if the final destination was already known, which it is not. Fourth, the continued uncertainty in the process means businesses and civil servants will again be left with little time to adapt to what will face them in January 2021 and must prepare for multiple outcomes.‘Transition’ has always been a misleading term, since it implies clarity about the destination to which the UK–EU relationship will be transitioning. The government’s red lines for that future relationship provide a sketch: outside of the single market and the jurisdiction of the European Court of Justice, with an independent trade policy and free movement ended.But businesses and civil servants are not likely to know until very late in the process if the basis for future trade with the EU will be in the form of a free trade agreement, to be negotiated and implemented by the end of the year, or no trade deal at all. This last outcome is a realistic prospect. Michel Barnier speaks in the European Parliament on 29 January. Photo: Getty Images. During withdrawal negotiations, the extensions were both unlimited in number and required decisions only at the last moment. In this phase, the talks may only be extended once, and that decision must be taken six months from the final deadline. It is difficult to see circumstances in which Boris Johnson agrees to break a political promise and manifesto pledge when he still has six more months to achieve his desired outcome.The UK, it is often noted, is already fully compliant with EU law and this shared starting point is often cited as a reason this negotiation will be simple, since the parties begin in alignment. But this novel negotiation will create new trade barriers in goods and services rather than remove them. Trade deals are often politically difficult since they create winners and losers. The Brexit negotiations, in terms of UK–EU trade at least, will generally create only different levels of losers, on both sides of the Channel.That means difficult politics, challenging negotiations and hard compromises, another reason to expect some ugly politics along the way and accept that failure is a plausible outcome.We do not yet know how Brexit will change Britain in the long term, whether a settled majority will ever come to view it as political folly or liberation, choice or inevitability. If its politically fragile union can withstand the pressures of the next few years, the UK may yet find a new stable position on the EU’s periphery and, after a period of economic adjustment, begin to address the many pressing domestic challenges which have suffered from neglect amid the all-consuming Brexit saga.But whatever happens in this next chapter, the EU can no longer be an excuse for national problems. As the UK takes back control it also returns accountability. In the future, there will be no one else to credit or to blame. Full Article
tia A single amino acid substitution uncouples catalysis and allostery in an essential biosynthetic enzyme in Mycobacterium tuberculosis [Enzymology] By feedproxy.google.com Published On :: 2020-05-08T03:41:14-07:00 Allostery exploits the conformational dynamics of enzymes by triggering a shift in population ensembles toward functionally distinct conformational or dynamic states. Allostery extensively regulates the activities of key enzymes within biosynthetic pathways to meet metabolic demand for their end products. Here, we have examined a critical enzyme, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS), at the gateway to aromatic amino acid biosynthesis in Mycobacterium tuberculosis, which shows extremely complex dynamic allostery: three distinct aromatic amino acids jointly communicate occupancy to the active site via subtle changes in dynamics, enabling exquisite fine-tuning of delivery of these essential metabolites. Furthermore, this allosteric mechanism is co-opted by pathway branchpoint enzyme chorismate mutase upon complex formation. In this study, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small-angle X-ray scattering, and X-ray crystallography analyses, we have pinpointed a critical node within the complex dynamic communication network responsible for this sophisticated allosteric machinery. Through a facile Gly to Pro substitution, we have altered backbone dynamics, completely severing the allosteric signal yet remarkably, generating a nonallosteric enzyme that retains full catalytic activity. We also identified a second residue of prime importance to the inter-enzyme communication with chorismate mutase. Our results reveal that highly complex dynamic allostery is surprisingly vulnerable and provide further insights into the intimate link between catalysis and allostery. Full Article
tia The Belt and Road Initiative: geo-economics and Indo-Pacific security competition By feedproxy.google.com Published On :: Wed, 08 Jan 2020 11:58:35 +0000 8 January 2020 , Volume 96, Number 1 Read online Mingjiang Li The Belt and Road Initiative (BRI) has been regarded by international society as a major policy tool in China's geo-economic strategy. Under this policy platform, Beijing has pledged to invest billions of dollars in the infrastructure and industrial sectors across Eurasia and in the Indo-Pacific nations. It is widely believed that such huge amount of investment will inevitably generate significant geostrategic repercussions in these regions. In response to the BRI, the United States and other powers have come up with a ‘free and open Indo-Pacific’ strategy. This article attempts to address the following question: what impact is the BRI likely to have on the security ties between China and the other major players in the Indo-Pacific? The author finds that the BRI may significantly transform China's international security policy and the expansion of Beijing's security influence may further intensify the security competition between China and other major powers in the Indo-Pacific region. The article also proposes a new analytical angle for the study of geo-economics that unpacks the role of economic activities and processes in generating geopolitical intentions and catalysing geopolitical competition. Full Article
tia The Smart Peace Initiative: An Integrated and Adaptive Approach to Building Peace By feedproxy.google.com Published On :: Tue, 11 Feb 2020 10:55:01 +0000 Invitation Only Research Event 12 May 2020 - 10:00am to 11:30amAdd to CalendariCalendar Outlook Google Yahoo Smart Peace brings together global expertise in conflict analysis and research, peacebuilding and mediation programming, and behavioural science and evaluation. Together, Smart Peace partners are developing integrated and adaptive peace initiatives, working with local partners to prevent and resolve complex and intractable conflicts in Central African Republic, Myanmar and northern Nigeria. This roundtable is an opportunity for Smart Peace partners to share the Smart Peace concept, approach and objectives, and experiences of the first phases of programme implementation. Roundtable discussions among participants from policy, practice and research communities will inform future priorities and planning for Smart Peace learning, advocacy and communication. Smart Peace partners include Conciliation Resources, Behavioural Insights Team, The Centre for Humanitarian Dialogue, Chatham House, ETH Zurich, International Crisis Group and The Asia Foundation. Nilza Amaral Project Manager, International Security Programme Email Department/project International Security Programme, Smart Peace Full Article
tia Soundscapes of war: the audio-visual performance of war by Shi'a militias in Iraq and Syria By feedproxy.google.com Published On :: Thu, 07 May 2020 09:16:54 +0000 7 May 2020 , Volume 96, Number 3 Helle Malmvig Read online This article sets out to bring sound and music to the field of visual studies in International Relations. It argues that IR largely has approached the visual field as if it was without sound; neglecting how audial landscapes frame and direct our interpretation of moving imagery. Sound and music contribute to making imagery intelligible to us, we ‘hear the pictures’ often without noticing. The audial can for instance articulate a visual absence, or blast visual signs, bring out certain emotional stages or subjects’ inner life. Audial frames steer us in distinct directions, they can mute the cries of the wounded in war, or amplify the sounds of joy of soldiers shooting in the air. To bring the audial and the visual analytically and empirically together, the article therefore proposes four key analytical themes: 1) the audial–visual frame, 2) point of view/point of audition, 3) modes of audio-visual synchronization and 4) aesthetics moods. These are applied to a study of ‘war music videos’ in Iraq and Syria made and circulated by Shi'a militias currently fighting there. Such war music videos, it is suggested, are not just artefacts of popular culture, but have become integral parts of how warfare is practiced today, and one that is shared by soldiers in the US and Europe. War music videos are performing war, just as they shape how war is known by spectators and participants alike. Full Article
tia Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein By feedproxy.google.com Published On :: 1997-11-01 DL BrasaemleNov 1, 1997; 38:2249-2263Articles Full Article
tia Harnessing the Potential of Cities By feedproxy.google.com Published On :: Fri, 22 Nov 2019 11:55:02 +0000 Corporate Members Event 13 December 2019 - 12:30pm to 2:00pm Chatham House | 10 St James's Square | London | SW1Y 4LE Event participants Penny Abeywardena, Commissioner for International Affairs, New York CityChair: Dr Beyza Unal, Senior Research Fellow, International Security Department, Chatham House Cities are strong engines of growth and job creation accounting for some 85 per cent of global GDP. By 2030, more than 60 per cent of people worldwide will live in cities and they will be on the front lines of managing issues transcending borders such as climate, migration and sustainability. International organizations like the United Nations are striving to meet these challenges but relying on traditional multilateral agreements is growing ever more unpredictable. If cities and local governments are key to global prosperity, how can their power and influence be harnessed to accelerate impact for their citizens?Drawing on the pioneering work of New York City in the area of foreign policy leadership at a municipal level, Penny Abeywardena will discuss how the influence of subnational leadership and diplomacy is more important than ever as national governments are abdicating their responsibility at the multilateral level. How can the Sustainable Development Goals be localized among cities on policy issues that impact communities? And what can large cities, beyond the capital, do to remain influential and harness job growth? This event will be preceded by an informal sandwich reception from 12.30pm-1pm. The roundtable discussion to follow will take place from 1pm-2pm.This event is corporate members only. If you'd like to attend, please RSVP to Linda Bedford.To enable as open a debate as possible, this event will be held under the Chatham House Rule. Members Events Team Email Full Article