Disaster relief funding for punishing spring floods is welcome news to civic leaders in northern Alberta.

Police are investigating a report of a suspicious package in north Edmonton.

Business owners throughout northern Ontario will have the chance Friday morning to speak directly with federal Economic Development Minister Mélanie Joly.

Those in Sudbury may have had a few choice words when waking up Friday morning after a snowfall overnight.

There are now 176 confirmed cases of COVID-19 in northeastern Ontario, and seven deaths.

The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Short linear epitopes may not be accessible to antibodies within the native tertiary and quaternary protein structures, but they become exposed upon the unraveling of proteins, exposing their secondary structure. Antibodies otherwise not suitable for the immunoprecipitation of proteins prepared under nondenaturing conditions are now able to bind these antigens of interest in cell lysates prepared under denaturing conditions. These antibodies may also work well for immunoblotting purposes when the protein target is completely denatured. Harvested cells in this protocol are washed in tris-buffered saline (TBS) before lysis in 2% sodium dodecyl sulfate (SDS)-containing Lysis buffer for 10 min at 100°C. The resulting sample is diluted 20-fold in TBS to reduce the SDS concentration to ≤0.1% before the addition of an antibody for immunoprecipitation. Addition of 2% bovine serum albumin (BSA) or 0.1% Nonidet P-40 to the TBS before an immunoprecipitation, respectively, ensures either removal of SDS from the target protein or retaining denatured proteins in solution.

Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly reduce nonspecific background in an immunoprecipitation by separation of cellular compartments into individual fractions. The lysis of cells by differential detergents permits the rapid extraction of proteins from the cytoplasm (digitonin), the cytoplasmic membranes, and organelles (Triton X-100), and nucleoplasm (Tween/DOC), facilitated through the use of distinct extraction buffers. Cytoplasmic and nuclear matrix proteins as well as DNA are left behind during the detergent-based extraction.

This rapid and efficient method to prepare highly purified recombinant adeno-associated viruses (rAAVs) is based on binding of negatively charged rAAV capsids to an anion-exchange resin that is pH dependent.

This is a simple method for rapid preparation of recombinant adeno-associated virus (rAAV) stocks, which can be used for in vivo gene delivery. The purity of these vectors is considerably lower than that obtained by either CsCl gradient centrifugation or by combination of iodixanol gradient ultracentrifugation followed by column chromatography.

The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.

Over many years, the Xenopus laevis embryo has provided a powerful model system to investigate how mechanical forces regulate cellular function. Here, we describe a system to apply reproducible tensile and compressive force to X. laevis animal cap tissue explants and to simultaneously assess cellular behavior using live confocal imaging.

Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

BIS Bulletin No 7, April 2020. Past epidemics had long-lasting effects on economies through illness and the loss of lives, while Covid-19 is marked by widespread containment measures and relatively lower fatalities among young people. The short-term costs of Covid-19 will probably dwarf those of past epidemics, due to the unprecedented and synchronised global sudden stop in economic activity induced by containment measures. The current estimated impact on global GDP growth for 2020 is around -4%, with substantial downside risks if containment policies are prolonged. Output losses are larger for major economies.

The National Book Festival Presents series was created to provide a book festival experience to lovers of the event on a year-round basis. Because these programs can no longer be held at the Library, we are offering a virtual multipart series, with authors who have written books about widespread diseases and the worldwide response to them.

No global real estate market despite higher price synchronisation and growing role of international investors, central banks find (Press release, 18 February 2020)

The Bank for International Settlements (BIS) today announced two key appointments to the BIS Innovation Hub, a new initiative designed to support central bank collaboration on new financial technology. (Press release, 19 February 2020)

Press release "Governors and Heads of Supervision announce deferral of Basel III implementation to increase operational capacity of banks and supervisors to respond to Covid-19", 27 March 2020

BCBS Press release "Basel Committee and IOSCO announce deferral of final implementation phases of the margin requirements for non-centrally cleared derivatives", 3 April 2020

Press release "Saudi G20 Presidency and the Bank for International Settlements (BIS) Innovation Hub invite global innovators to solve RegTech and SupTech challenges", 27 April 2020

It works like a very fine "molecular knob" able to modulate the electrical activity of the neurons of our cerebral cortex, crucial to the functioning of our brain.

The American Geriatrics Society annually honors researchers, clinicians, educators, and emerging health professionals who have made outstanding contributions to high-quality, person-centered care for older people.

Imagining America between the crisis and the cure.

In a pandemic, it’s not just truth against misinformation. Every choice is an experiment.

The last time Charlene Chiddenton saw her father in person was on March 7 — visitor restrictions at long-term care homes during the pandemic had kept them apart — and less than two months later, she was saying goodbye to her father by video call.

Fifty thousand jobs were lost in Nova Scotia in April, reflecting the devastating economic impact of the first full month of public health orders to prevent the spread of COVID-19.

The only daily TV news package to focus on Nova Scotians and their stories

A Dalhousie University scientist and a NSCAD textile professor are teaming up to create a new fabric. It could be used to locally manufacture personal protective equipment like surgical masks. As the CBC's Colleen Jones reports, they have received a COVID-19 grant to study the idea.

A number of fishing zones in the Gulf of St. Lawrence have been closed after North Atlantic right whales were sighted earlier this week.

What would have been Cape Breton University's largest convocation to date is being replaced by an online offering this year because of COVID-19. Some graduating students say it just won't be the same as a traditional ceremony.

A woman who lost her sister to COVID-19 at the start of the month is thanking the staff at Northwood who took "excellent" care of her in her final days. Jean Harrigan, 90, is being remembered for her caring and lively nature.

As Nova Scotians gazed up at the Canadian Forces Snowbirds soaring through the clouds last weekend, Matt MacKenzie was looking down and feeling the weight of successive tragedies that had enveloped his home province. 

Over the 13 hours the mass shooting took place across Nova Scotia on April 18 and 19, the RCMP called in resources from New Brunswick to help. However, the two municipal police forces closest to Portapique were not asked to respond directly at any point, CBC News has learned.

The latest death was at the Northwood long-term care home in Halifax.

For those working in CHSLDs — the province's long-term care homes which are the site of the most serious outbreaks — the bonus will amount to as much as $1,000 per month.

Grim projections, travel rules and feeling safe while shopping at your local market — here's what's going on today.

History’s census enumerators came back with the numbers and some very tall tales

The National Museum of American History will undergo a dramatic makeover

Discover the real George Washington through his restored home and gardens, over 1,000 artifacts, and an immersive Revolutionary War "snow" experience

Making Tierra Mía, says the director of the Smithsonian Latino Center, proved transformative in giving voice to the people

Hope you didn't pack away those winter boots yet: southwestern Manitoba, from Riding Mountain south to the Canada-U.S. border, is expected to get heavy rain and snow Friday night.

Winnipeg police officers who arrested a man who then went into medical distress and later died were not at fault, an investigation by Manitoba's police watchdog has found.

Manitoba municipalities will be receiving most of their operating funding from the province sooner than usual because of the COVID-19 pandemic, the province announced Friday.

We can expect to see more seasonable temperatures by next weekend, says a meteorologist from Environment Canada.

Winnipeg will soon test "small cell" technology to improve cellular reception in parts of Winnipeg, ahead of a possible future transition to 5G service. 

Retailers around the world are adjusting their business models in the face of COVID-19 social distancing requirements. Two consumer reports on shopping pattern changes during the pandemic offer two main findings: a) It is crucial for merchants to create ways to connect with new and long-term customers; and b) Consumers can not fully solve the empty shelf problem through buying online.