According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.

The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.

The present invention relates to the generation of a mucin-producing cell using stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells.

The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.

Monoclonal antibodies (mAbs) having thyroid stimulating activity (TSAb), especially full or considerably agonistic activity, or thyroid blocking activity (TBAb), which are obtainable by genetic immunization of mice, or fragments (F(ab')2, Fab or Fv) or humanized forms of such monoclonal antibodies or single chain forms (SCA; scFv) of such fragments, which antibodies, or their fragments, compete with bovine TSH for epitopes of the human TSHr, compete with autoantibodies from sera from Graves' patients as well as with autoantibodies from sera from patients harboring blocking autoantibodies for epitopes of the human TSHr, bind to conformational epitopes of the human TSHr located in the first 281 amino acids of the human TSHr, and usually also bind to TSFR receptors (TSHr) from different animals. Various uses of such antibodies, or of peptides corresponding to variable regions of such antibodies, are also described and claimed.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The invention relates to genes coding for TCP family transcription factors and having a biological role in the development of axillary buds and branch growth. Furthermore, the invention relates to the promoters of the transcription of said genes, to the genetic constructs containing same and to the uses thereof, including the use of agents that modulate the expression of these genes in order to modify plant architecture.

The present invention relates to a composition useful for the diagnosis of diseases associated with aberrant expression of the genes encoding the secreted proteins Futrin 1, 2, 3 and/or 4(=R-Spondin 2, 3, 1 and 4, respectively), e.g. in connection with tumors or diseases of the muscle, kidneys or bones. The present invention also relates to a pharmaceutical composition containing a compound which is capable of modifying (a) the expression of the gene encoding Futrin 1, 2, 3 and/or 4 or (b) the activity of the Futrin 1, 2, 3 and/or 4 protein.

The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.

An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

Disclosed are process for contacting a protein containing material with one or more wet-mill streams. The protein content of the protein containing material is increased.

The invention relates to malodor controlling bacteria and related methods and compositions for the control and prevention of malodor.

The present invention relates, in general, to gene expression and, in particular, to a method of inhibiting the expression of a target gene and to constructs suitable for use in such a method.

[Problem to be Solved] The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

A vehicle seat can include a seat bottom frame having a front, a back opposite the front, and enclosing a volume. Pivotally connected to the back of the seat bottom frame can be a seat back frame and pivotally connected to the front of the seat bottom frame can be a seat leg rest. The seat leg rest has a length. Mounted in the volume of the seat frame are at least two leg rest springs which are connected to the leg rest. When the leg rest springs are actuated, the seat leg rest can be directly pivoted approximately and incrementally parallel with the seat bottom frame. The two leg rest springs are mounted a first distance apart and the first distance is less than half of the length of the leg rest.

A premium class passenger suite that includes a main seat positioned in the suite together with separate bed. The bed has a flexible mattress of predetermined dimensions and is movable between a stowed position to one side of the main seat and a deployed position above and separate from the main seat. A drive apparatus is provided for driving the bed between the stowed and deployed positions. The main seat is configured to be movable between a seating position when the bed is stowed, and a stowed position with a lowered seat back when the bed is deployed for use.

A method of making a vehicle seat assembly comprising providing a cushion having an “A” surface, a “B” surface, a central portion, and two bolster areas, with each bolster area being adjacent the central portion, the cushion having a plurality of intrusions extending from the “A” surface towards the “B” surface to form a hardness gradient between at least one of the bolster areas and the central portion of between 8% to 25%, securing the cushion to a frame, and covering cushion with a trim member.

There is provided a moveable power seat slide device assembly comprising a movable seat cushion and seat rail including upper rails secured to the seat cushion and lower rails secured to the body of the golf cart, a motor for driving each movable seat and a current control circuit. The prostate golf cart seat protects the prostate area from continuous bouncing. The seat has two 8 inch stationary end cushions. The center would consist of two 8 inch cushions moving along a rail which allows riders to automatically close the 7 inch seat gap to 3 inches from the stationary end cushion. The center air space between each rider and seat would have a depth 1½ inches.

The present invention provides an apparatus for enhancing Q factor of an inductor. The apparatus includes a negative resistance generator coupled to the inductor for providing a negative resistance, and a bias circuit coupled to the negative resistance generator for biasing the negative resistance generator.

A plurality of varactors are coupled via a first electrode to a shared terminal that in turn can operably couple to a source of control voltage. A second electrode for each varactor couples to a corresponding switch, where each switch couples to at least two different voltage levels. So configured, the second electrode of each varactor can be individually connected to either of two voltage levels. This can be leveraged to control, in coarse steps, the overall aggregate effective capacitance presented by these components. At least some of these varactors can have differing corresponding capacitances, the specific values of which can be selected in order to facilitate relatively equal spacing and substantially equal rates of reactance change versus the control voltage value between aggregate-capacitive reactance ranges as correspond to differing settings for the switches at various levels for the control voltage source.

An embodiment of the present invention provides an apparatus, comprising at least one anti-parallel pair VVC network comprised of two parallel VVCs with one biased in the opposite polarity of the other and at least one anti-series VVC network comprised of two VVCs configured in series, one biased in the opposite polarity of the other such that the resulting AC capacitive variations produce a desired capacitance variation.

A tuner and a transformer formed by printed circuit board thereof are provided. The transformer includes a first winding and a second winding. In which, the first winding forms a first inductor and the second winding forms a second inductor. The transformer is formed by the first and the second inductors, wherein the first winding and the second winding are formed by conducting wires of a printed circuit board.

Remote programmable reference systems and methods are described. A remote programmable reference system maintains a set of parameters from which a reference signal can be generated to transmit the reference signal to a system platform. In an embodiment, a parameters update can be received from which a modified reference signal can be generated to transmit the modified reference signal. In another embodiment, the remote programmable reference system maintains multiple sets of parameters from which respective reference signals can be generated for any one or more system platforms having different system configurations. The system platforms can interface with the remote programmable reference system through a system interface via a communication network.

A printing apparatus, having at least two platens, at least one print head, at least one print head drive unit to drive the print head in a main scanning direction, at least two platen drive units to drive the platens in an auxiliary direction, at least one ink cartridge, at least two operation input units to be operated by an operator to instruct the printing apparatus to activate a first printing process and a second printing process respectively, and a cooperation controller system to control the print head, the print head drive unit, the first platen drive unit, and the second platen drive unit to behave in cooperation with one another so that the second printing process is started one of after and during the first printing process when an instruction to start the second printing process is entered whilst the first printing process is in progress, is provided.

A pattern printing system and data processing method thereof are disclosed, which are suitable for printing patterns on printed circuit boards or data format rearrangement printing used in displays. The pattern printing method includes a process for interpreting scription data into matrix data, a procedure for modulating the print head resolution and the printing resolution, a procedure for interpreting and transmitting data commands, a procedure for rearranging memory data, and a procedure for firing data synchronously so as to achieve high-resolution printing and to continuously modulate any print data.

A handy printer and a mobile phone based on the use of the same are provided, wherein the printing can be performed on recording paper sheets having various sizes. The handy printer comprises a cover member which is movable between an exposure position at which an ink discharge surface of an ink discharge head is exposable to the outside and a cover position at which the discharge surface is hidden from the outside. When the cover member is positioned at the cover position, the recording is permitted on a recording paper sheet accommodated in a recording paper-accommodating mechanism. When the cover member is positioned at the exposure position, the recording is permitted on a recording medium disposed outside a handy-printer body casing.

One of mark sensors is automatically selected in accordance with specifications of a thermal sheet. A cutting position on the thermal sheet is decided on the basis of an output of the selected one mark sensor.

A print shop consolidation system including a print shop consolidation management system with an application is provided. The application is used to (1) evaluate, with a set of information, an operational capacity of a first print shop to process both a first group of print jobs and a second group of print jobs, (2) evaluate, with the set of information, an operational capacity of the second print shop to process both the first and second groups of print jobs, and (3) use the evaluations of (1) and (2) to consolidate processing of the first and second groups of print jobs at one of first and second print shops.

An object of this invention is to provide a newspaper production system, having the structure of a system that is provided with a supply unit for a continuous web provided at an upstream side of a printing unit for printing on one surface and the other surface of a continuous web, and a processing unit for carrying out cutting and folding processing of a continuous web after printing at a downstream side, by making the area of an installation flat region for the printing unit for printing on one surface and the other surface of a continuous web, having an ink jet printing device group for printing one side of the continuous web and an ink jet printing device group for printing the other side of the paper sheet, small, and with which it is easy for a single person to carry out a newspaper production operation of monitoring the entire system. It is also an object of this invention to provide a newspaper production system and newspaper production method that enables production of a few sizes of a plurality of newspaper pages arranged horizontally as sheets constituting a newspaper by sequentially cutting, and being capable of producing a newspaper formed of various page configurations.

A pen which is able to modulate the trace of the pen in response to a sensory signal of the user, such as for example skin conductance or respiration. The sensory signals represent the mood of the user. By changing the trace of the pen, a change in mood of the user can be expressed.

A data processing apparatus is monitoring the first port of itself and the second port of itself. If the data processing apparatus receives data via the second port, it conducts a specific process to the data regardless of a data format of the data so as to convert the data into the data format that can be processed regardless of a data format of the data and sends converted data to the first port.

A method enables changes in the operation of a web printing system in response to web dimensional changes being detected. The method includes identifying a cross-process dimensional change in a web moving through a printing system, comparing the identified cross-process dimensional change to a predetermined threshold, and changing operation of a component in the web printing system in response to the cross-process dimensional change exceeding the predetermined threshold.

A host apparatus used in a printing system includes the host apparatus and an image forming apparatus, and the image forming apparatus receives print data and authentication information from the host apparatus so as to perform authentication operation referring to the authentication information. The host apparatus includes: an authentication information obtaining unit configured to obtain login authentication information from an operating system of the host apparatus as the authentication information, wherein the login authentication information obtained from the operating system is one entered when a user logs in to the host apparatus or to a network domain; and an authentication information sending unit configured to send the login authentication information to the image forming apparatus as the authentication information.

An apparatus and method that operates a flattener in an ink-based printing apparatus. The printing apparatus can include a media path configured to transport media sheets. The printing apparatus can include a marking module configured to jet ink drops to generate images on the media sheets. The printing apparatus can include a flattener configured to flatten the ink jet drops of the images on the media sheets in a flattener nip. The printing apparatus can include a release agent distributer configured to distribute release agent on a first rotational flattener member. The printing apparatus can include a controller configured to control the printing apparatus to reduce an inter-copy gap distance between a first media sheet and a second media sheet to prevent a first rotational flattener member from contacting a second rotational flattener member between the first media sheet and the second media sheet.

Systems and methods are provided for installing workflow servers for a print shop. One embodiment is a system that installs a workflow server for a print shop. The system accesses installation templates, configured for different categories of print shop, that describe a set of print shop resources expected to exist at the category of print shop. The system also installs workflow server software on a computer system of a print shop. During the installation, the system determines a category for the print shop, selects an installation template that matches the category of the print shop, and performs a dialogue of queries with a user to determine which resources of the set of print shop resources identified by the installation template are available to the print shop, wherein the dialogue excludes queries for print shop resources that are not in the set of print shop resources described by the installation template.

A printing system that has a printhead assembly for printing media fed along a media path and a plurality of service modules for the printhead assembly. Each of the service modules is configured to operate in a plurality of different modes and each of the service modules are independently operable.

The invention provides an apparatus including: a rotatable belt having an outer surface and an inner surface, the outer surface partially coming into contact with a sheet; a plurality of rollers being arranged in a direction of movement of the sheet and including a first roller and a second roller adjacent to each other, the plurality of rollers being pressed against the outer surface and the moving sheet being nipped between the plurality of rollers and the outer surface; a blowing mechanism configured to blow hot air from between the first roller and the second roller toward the sheet; and a heating unit including a heater and a contact surface coming into contact with the inner surface, the heating unit being configured to be able to set the contact surface thereof to have a predetermined non-uniform temperature distribution.

A method for producing printed articles that encompasses providing a first ink composition that contains metal oxide particles with an average particle size in the range of about 3 to about 300 nm; providing a second ink composition that contains colored pigments dispersed in an ink vehicle; providing a printable media having a bottom supporting substrate and an ink-absorbing layer with pore diameters that are smaller than the size of the metal oxide pigment particles and colored pigments; and sequentially applying, on the same print area, the first and the second ink compositions onto said printable media. Also disclosed herein are the printed articles obtained according to the present method.

A container data center includes a container, a plurality of power supplies, an electric switch system, a plurality of normally closed switches, an alarm device, and a controller. The electric switch system is received in the container. The plurality of normally closed switches are connected between the power supplies and the electric switch system. Each normally closed switch is connected with a corresponding one of the power supplies in series. The controller is connected to the normally closed switches and the alarm device, configured for receiving earthquake information containing an earthquake intensity, and configured for controlling the alarm device to activate alarms and controlling some of the normally closed switches or all the normally closed switches to open when the earthquake intensity is equal to or greater than a predetermined earthquake intensity.

A printing device includes at least one printing engine that has actuators and sensors. At least one engine controller is operatively connected to the printing engine, the engine controller uses software to control operations of the printing engine. At least one non-volatile memory is operatively connected to the engine controller. The non-volatile memory stores values used by the engine controller to control operations of the printing engine. Further, at least one adapter card is operatively connected to the non-volatile memory and to the actuators and sensors. The adapter card stores data and receives sensor feedback from the sensors. The adapter card uses the data and the sensor feedback to control the actuators by bypassing the engine controller when communicating with the actuators. The adapter card provides adapter card feedback to the non-volatile memory.

The inside of a housing of a printer device is cooled by the air from a ventilation port and, consequently, the temperature of solder SP on a mask surface supported by a mask holding frame inside the housing can be kept low. Moreover, provided is a mask cover for blocking the air flow from the ventilation port toward the mask, and the drying of the solder SP caused by the wind from the ventilation port is thereby inhibited as a result of the wind from the ventilation port not coming into contact with the solder SP on the mask surface. In this way, the solder SP is cooled while inhibiting the drying of the solder SP and, consequently, it is possible to appropriately inhibit the change in viscosity of the solder SP, and favorable printing can be realized.

A time recorder includes a first sensor that detects the side edge of a time card having a cut-out formed at at least one corner of the bottom, a second sensor that detects the bottom of the time card, and a card feeding unit that feeds the time card. When the time card is fed by this card feeding unit, a pulse counter of the card feeding unit counts the number of pulses of predetermined pulse signals after the first sensor detects the time card and until the second sensor detects the time card. Next, the front and back faces of the time card are determined based on the number of pulses that is a counting result. Hence, the front and back faces can be determined by the first sensor and the second sensor only.

After a reducing agent is added to a water reaction system containing silver ions to deposit silver particles by reduction, the silver particles are dried to obtain a silver powder which is heat-treated at a temperature of higher than 100° C. and lower than 400° C. The silver powder thus heat-treated has a maximum coefficient of thermal expansion of not greater than 1.5% at a temperature of 50° C. to 800° C., and has no heating peak when the silver powder is heated from 50° C. to 800° C. The silver powder has an ignition loss of not greater than 1.0% when the silver powder is ignited until the weight of the silver powder is constant at 800° C. The silver powder has a tap density of not less than 2 g/cm3 and a BET specific surface area of not greater than 5 m2/g.

New 6xxx aluminum alloy bodies and methods of producing the same are disclosed. The new 6xxx aluminum alloy bodies may be produced by preparing the aluminum alloy body for post-solutionizing cold work, cold working by at least 25%, and then thermally treating. The new 6xxx aluminum alloy bodies may realize improved strength and other properties.

Nanostructured Mn—Al, Mn—Al—C permanent magnets are disclosed. The magnets have high coercivities (about 4.8 kOe and 5.2 kOe) and high magnetization values. An intennetallic composition includes a ternary transition metal modified manganese aluminum alloy Mn—Al—Fe, Mn—Al—Ni, or Mn—Al—Co having at least about 80% of a magnetic τ phase and permanent magnetic properties. The alloy may have a saturation magnetization value of at least 96 emu/g with approximately 5% ternary transition metal replacing Al. The alloy may also have a saturation magnetization value of at least 105 emu/g with 10% ternary transition metal replacing Al.

In a reflow process, a plurality of solder bumps between a first workpiece and a second workpiece is melted. During a solidification stage of the plurality of solder bumps, the plurality of solder bumps is cooled at a first cooling rate. After the solidification stage is finished, the plurality of solder bumps is cooled at a second cooling rate lower than the first cooling rate.

Provided are a bake hardening steel having a crystalline grain size of ASTM No. 9 or more and a method for preparing the bake hardening steel by controlling the winding, rolling and cooling conditions. The bake hardening steel includes: C:0.0016˜0.0025%, Si:0.02% or less, P:0.01˜0.05%, S:0.01% or less, sol.Al:0.08˜0.12%, N:0.0025% or less, Ti:0.003% or less, Nb:0.003˜0.011%, Mo:0.01˜0.1%, B:0.0005˜0.0015% or less, balance Fe and other inevitable impurities, wherein % is weight %, and Mn and P satisfy the relation of −30(° C.)≧803P−24.4Mn−58.

Basic zinc cyanurate fine particles are produced by subjecting a mixed slurry to wet dispersion using a dispersion medium at a temperature in the range of 5 to 55° C., the mixed slurry being formed by blending water, cyanuric acid, and at least one component selected from zinc oxide and basic zinc carbonate such that the cyanuric acid concentration is 0.1 to 10.0 mass % with respect to water.

The present invention provides a sputtering target suitable for producing an amorphous transparent conductive film which can be formed without heating a substrate and without feeding water during the sputtering; which is easily crystallized by low-temperature annealing; and which has low resistivity after the crystallization. An oxide sintered compact containing an indium oxide as a main component, while containing tin as a first additive element, and one or more elements selected from germanium, nickel, manganese, and aluminum as a second additive element, with the content of tin which is the first additive element being 2-15 atom % relative to the total content of indium and tin, and the total content of the second additive element being 0.1-2 atom % relative to the total content of indium, tin and the second additive element.