ac

Heat Exchange Catheters and Their Methods of Manufacture and Use

A closed loop catheter useable for heat exchange is manufactured by forming a plurality of generally transverse bore holes though a flexible, multilumen catheter body, lacing a tube trough the bore holes so that loops of the tube protrude from the catheter body, connecting one end of the tube to an inflow lumen of the catheter and connecting the other end of the tube to an outflow lumen of the catheter. A heated or cooled heat exchange medium may then be circulated through the tube while the catheter is inserted in the vasculature of a subject, thereby resulting in heat exchange between the subject's flowing blood and the heat exchange medium being circulated through the tube.




ac

INTRODUCER FOR ACCESSING CORONARY SINUS VIA RIGHT PARASTERNAL MEDIASTINOTOMY

An introducer configured with a first curve having a first angle that traverses space of an atrial appendage, a central atrium, caudad to the coronary sinus, and a second curve that has an angle sufficient to align the introducer with an intrinsic curvature of the coronary sinus of a subject.




ac

Reduced-Friction Catheter Introducer and Method of Manufacturing and Using the Same

A sheath introducer for a catheter includes a sheath having a lumen, a hub positioned on a proximal end of the sheath, and a housing positioned on the hub. The hub can include a splittable penetration member having a port in fluid communication with the sheath lumen. The housing can include a valve having a closed upper surface and a channel surrounding the splittable penetration member. Movement of the housing with respect to the hub can expose the port of the splittable penetration member for insertion of the catheter.




ac

BIOSYNCHRONOUS TRANSDERMAL DRUG DELIVERY FOR LONGEVITY, ANTI-AGING, FATIGUE MANAGEMENT, OBESITY, WEIGHT LOSS, WEIGHT MANAGEMENT, DELIVERY OF NUTRACEUTICALS, AND THE TREATMENT OF HYPERGLYCEMIA, ALZHEIMER'S DISEASE, SLEEP DISORDERS, PARKINSON'S DISE

Systems and methods for longevity, anti-aging, fatigue management, obesity, weight loss, weight management, delivery of nutraceuticals, and treating hyperglycemia, Alzheimer's disease, sleep disorders, Parkinson's disease, Attention Deficit Disorder and nicotine addiction involve synchronizing and tailoring the administration of nutraceuticals, medications and other substances in accordance with the body's natural circadian rhythms, meal times and other factors. Improved control of blood glucose levels, extended alertness, and weight control, and counteracting of disease symptoms when they are at their worst are possible. An automated, pre-programmable transdermal administration system is used to provide pulsed doses of medications, pharmaceuticals, hormones, neuropeptides, anorexigens, pro-drugs, stimulants, nutraceuticals, phytochemicals, phytonutrients, enzymes, antioxidants, essential oils, fatty acids, minerals, vitamins, amino acids, coenzymes, or other physiological active ingredient or precursor. The system can utilize a pump, pressurized reservoir, a system for removing depleted carrier solution, or other modulated dispensing actuator, in conjunction with porous membranes or micro-fabricated structures.




ac

METHOD AND APPRATUS FOR TREATMENT OF INTRACRANIAL HEMORRHAGES

An ultrasound catheter with a lumen for fluid delivery and fluid evacuation, and an ultrasound source is used for the treatment of intracerebral or intraventricular hemorrhages. After the catheter is inserted into a blood clot, a lytic drug can be delivered to the blood clot via the lumen while applying ultrasonic energy to the treatment site. As the blood clot is dissolved, the liquefied blood clot can be removed by evacuation through the lumen.




ac

MEDICAL ACCESS PORT, SYSTEMS AND METHODS OF USE THEREOF

A medical device comprising a subcutaneous access port having an access port body and at least one needle having a removable needle tip and a needle shaft defining a needle lumen; the at least one needle housed within the access port body, the at least one needle extendable and retractable relative to the access port body; and a needle shift mechanism operable such that the at least one needle is extendable from and retractable into the access port body at a plurality of positions of the access port body.




ac

MEDICAL PORT WITH REPLACEABLE CATHETER

The present disclosure is directed to a medical port drainage assembly that includes a port component and a replaceable catheter. The port component includes a tube having a proximal end, a distal end, and tube walls defining a catheter access lumen. A head component is located on a proximal end of the tube and an assembly retention element is located on a distal end of the tube. The head component is deployed outside the patient's body and defines an opening to the catheter access lumen. The assembly retention element is deployed within a lumen of the patient's body. The port component includes a valve assembly which may be a single valve (e.g., a duckbill valve) or a system of valves. The port component also includes a connector for coupling and decoupling the replaceable catheter to the port component.




ac

ENHANCEMENT OF PENETRATION AND ACTION

The present invention relates, inter alia, to dermal systems comprising at least one light source and at least one pharmaceutically and/or cosmetically active ingredient. The dermal systems can be employed in order to enhance the penetration and action of the pharmaceutically and/or cosmetically active ingredient.




ac

METHODS AND SYSTEMS FOR ACCURACY IMPROVEMENT IN CURRENT COMPARATORS

Precision AC and DC voltage, current, phase, power and energy measurements and calibrations with current ranges from 1 uA to 20 kA and voltage ranges from 1V to 1000 kV are now performed with accuracies of better than one part per million. Continued demand for improved accuracy has led the inventors to address remnant magetization within the current comparators that form the basis of the measuring process within many of the measurement instruments providing the precision AC and DC measurements and calibrations. Accordingly, the inventors present current comparator and measurement system architectures together with control protocols to provide for correction of this remnant magnetization.




ac

CAPACITANCE DETECTION APPARATUS AND INPUT APPARATUS

A voltage of a first capacitor is compared with a threshold voltage and a signal corresponding to the comparison result is generated at each of a first timing during a period after the transfer of a positive charge has ended and before the transfer of a negative charge starts and a second timing during a period after the transfer of the negative charge has ended and before the transfer of the positive charge starts. In each of the case where a positive charge is transferred and the case where a negative charge is transferred, operations (digitization of an integrated value and a feedback operation) of a delta sigma modulator are performed.




ac

ELECTRONIC ARRANGEMENT AND VECTOR NETWORK ANALYZER CHARACTERIZED BY REDUCED PHASE NOISE

An electronic arrangement and method for providing a signal characterized by reduced phase noise having a signal source for providing a stimulus signal, a modulator coupled to the signal source for generating a modulated signal as function of the stimulus signal and a local oscillator signal, and a mixer combining the stimulus and modulated signals to generate a mixed signal that includes a component characterized by a mathematical difference of the stimulus signal and the modulated signal. The modulated signal is substantially identical to the stimulus signal and offset by a frequency of the local oscillator signal, so that the difference component of the mixed signal results in a local oscillator signal wherein the stimulus signal phase noise generated by the signal source has been mathematically cancelled.




ac

METHOD OF DETECTING AN ELECTRIC ARC BY ANALYSIS OF ITS ACOUSTIC SIGNATURE

A method of detecting an electric arc in an electrical system from a signal originating from at least one sensor detecting acoustic waves in the system, including: a) calculating by means of a processing device, over a sliding window of signal samples, at least one statistical parameter selected from the skewness and the kurtosis of the signal; b) detecting a possible occurrence of an event by taking into account said at least one statistical parameter; and c) performing a frequency analysis of the signal enabling to identify an electric arc when an event is detected at step b).




ac

QUALITY EVALUATION METHOD FOR LAMINATE HAVING PROTECTIVE LAYER ON SURFACE OF OXIDE SEMICONDUCTOR THIN FILM AND QUALITY CONTROL METHOD FOR OXIDE SEMICONDUCTOR THIN FILM

Provided is a method for simply evaluating defects caused in interface states in oxide semiconductor thin films and protective films in TFTs having protective films formed on the surface of oxide semiconductor thin films without actually measuring the characteristics of the same. This evaluation method evaluates defects caused in the interface states by measuring electron states in the oxide semiconductor thin film by a contact method or noncontact method. The defects caused in the interface states are any of the following (1)-(3). (1) Threshold value voltage (Vth,) when a positive bias is applied to the thin-film transistor(2) Difference in threshold value voltage (ΔVth) before and after applying the positive bias to the thin-film transistor(3) Threshold value during the first measurement when a plurality of measurements is made of the threshold value voltage when a positive bias is applied to the thin-film transistor.




ac

PIEZOELECTRIC MATERIAL COMPRISING POLY(D-LACTIC ACID)/POLY(L-LACTIC ACID) STEREOCOMPLEX CRYSTALS

Disclosed is a piezoelectric material including stereocomplex crystals of poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA), wherein poly(D-lactic acid) and poly(L-lactic acid) are crystallized into a stereocomplex, thus exhibiting superior heat resistance and piezoelectric properties. This piezoelectric material, which is obtained using poly(lactic acid), can be produced at very low cost compared to when using PVDF, and is configured to include stereocomplex crystals of PDLA and PLLA and can thus manifest high thermal stability and piezoelectric properties, compared to when using conventional PLLA alone. Such a piezoelectric material can be efficiently utilized in a variety of fields in which low production cost, high processing temperature in the manufacturing process, or high-temperature stability of piezoelectric material products is required.




ac

MEASURING FREQUENCY-DEPENDENT ACOUSTIC ATTENUATION

Methods and systems for frequency-dependent fluid attenuation measurement are provided. In certain embodiments, the methods comprise: generating one or more reference acoustic signals within a reference fluid; receiving one or more reference acoustic signal reflections; determining a frequency-dependent response function based, at least in part, on the one or more reference acoustic signal reflections; generating one or more sample acoustic signals within a sample fluid; receiving one or more sample acoustic signal reflections; and determining a frequency-dependent attenuation function of the sample fluid based, at least in part, on the one or more sample acoustic signal reflections and the frequency-dependent response function.




ac

ROTATING MACHINE ABNORMALITY DETECTION DEVICE, ROTATING MACHINE ABNORMALITY DETECTION METHOD, AND ROTATING MACHINE

An abnormality detecting apparatus (10) for a rotating machine includes: a calculating part (15) which calculates a phase difference between signals respectively output from two sensors of one or more sets, the one or more sets being predetermined combinations of two sensors among the plurality of sensors, the plurality of sensors each of which detects an elastic wave generating in the rotating machine (1) having a rotor during rotation of the rotor, the sensors being arranged at predetermined different locations of the rotating machine (1); a storing part (16) which stores in advance information concerning a relationship between a phase difference and a contact position when a contact occurs during the rotation of the rotor, regarding the one or more sets; and a specifying part (17) which specifies a contact position by using a phase difference calculated by the calculating part (15) and the information stored in the storing part (16).




ac

SENSOR ARRANGEMENT AND MEASUREMENT METHOD FOR A TURBOMACHINE

A sensor arrangement with a sensor element for measuring at least one physical and/or chemical fluid characteristic in a turbomachine is provided. The sensor element detects the at least one fluid characteristic inside a non-contact seal, in particular a labyrinth seal, between a rotor stage and a stator stage, wherein during operation the sensor element is in contact with the fluid flow along the flow path inside the labyrinth seal.




ac

TRACK VEHICLE COLLISION TESTING DEVICE AND TRACK VEHICLE COLLISION TESTING METHOD

A rail vehicle collision test rig used for a collision test on a tested vehicle is provided, and includes: a track configured to support and guide the tested vehicle; a small vehicle configured to push the tested vehicle; a drive motor configured to drive the small vehicle to advance and arranged at a first end of the track; a first rotating hub arranged at the first end of the track and connected to the drive motor; a brake motor configured to brake the small vehicle; a second rotating hub arranged at the second end of the track and connected to the brake motor; a connecting rope wound on the first rotating hub and the second rotating hub; a detector configured to detect a velocity and a position of the tested vehicle; and a controller connected to the detector and is allowed to be in communication with the detector.




ac

VACUUM-ASSISTED IN-NEEDLE CAPPLICARY ADSORPTION TRAP WITH MULTIWALLED POLYANILINE/CARBON NANOTUBE NANOCOMPOSITE SORBENT

A vacuum-assisted in-needle capillary adsorption trap (VA-INCAT) device for sampling and delivering materials to an analytical device is disclosed. A sorbent is multiwall carbon nanotube/polyaniline (PANI/MWCNT) nanocomposite and is coated within an interior space of the needle between the second end and the side aperture to entrap an analyte within a sample. The VA-INCAT device also includes a vacuum device configured to vacuum the vacuum flask to improve the extraction of the analytes vapors from the sample matrix to the sorbent bed.




ac

APPARATUS, METHOD, AND SYSTEM FOR DETECTING ACCELERATION AND MOTOR MONITORING

Described is a system which includes: a cable including: a first fiber optic interconnect to provide an input light; and a second fiber optic interconnect to provide an output light; and a first housing coupled to the cable, the first housing including: a first deflection circuit to deflect the input light received from the first fiber optic interconnect in response to a vibration or movement of the first housing; and a second housing coupled to the cable, the second housing including: a light source to generate the input light for transmission to the first deflection circuit via the first fiber optic interconnect; and a photo detector to receive the output light from the first deflection circuit via the second fiber optic interconnect.




ac

SUBSTRATE FOR SENSOR, PHYSICAL QUANTITY DETECTION SENSOR, ACCELERATION SENSOR, ELECTRONIC APPARATUS, AND MOVING OBJECT

A substrate for a sensor includes: a base section; a movable section connected to the base section; an arm portion as a support portion extending along the movable section from the base section; a first gap portion having a protrusion portion in which one of the movable section and the arm portion protrudes toward the other of the movable section and the arm portion, and having a predetermined gap between the protrusion portion on one side and the other of the movable section and the support portion; and a second gap portion which is located further toward the base section side than the first gap portion has a gap wider than the predetermined gap, in which in the first gap portion, one of the movable section and the arm portion has a ridge portion on the side facing the first gap portion.




ac

EUV LPP SOURCE WITH IMPROVED DOSE CONTROL BY TRACKING DOSE OVER SPECIFIED WINDOW

A method and apparatus for controlling a dose of extreme ultraviolet (EUV) radiation generated by a laser produced plasma (LPP) EUV light source. In one embodiment, a running total of the EUV energy generated over a predetermined number of laser pulses is measured; once that number of pulses is exceeded, the energy from the pulse immediately preceding the most recent predetermined number of pulses is dropped from the running total, so that the running total is from the most recent predetermined number of pulses. If the running total of the EUV energy exceeds a target dose, the next pulse is caused to not hit a droplet. This avoids the unwanted side effects of various prior art solutions, such as needing to miss many droplets in a row, or requiring the laser pulses to be shortened or reduced in power as in other prior art solutions.




ac

VEHICLE TRACTION SYSTEM AND RADIATION IMAGING CHECK SYSTEM

The present disclosure relates to vehicle traction apparatus and radiation imaging check systems. One illustrative implementation may comprise two parallel tracks, two sets of traction mechanisms and a driving unit, wherein the tracks are disposed on a ground. The two sets of traction mechanisms may be respectively disposed on the two tracks. Further, the driving unit may be adapted for driving the two sets of traction mechanisms to synchronously move along the two tracks. In some embodiments, each of the two sets of traction mechanisms includes a body, a cantilever, a lifting driving mechanism and a wheel supporting assembly, and may include features such as the body being mounted on the track, the cantilever being disposed parallel to a direction of the tracks, and/or both ends of the cantilever being respectively connected with the lifting driving mechanism and the wheel supporting assembly.




ac

ACTIVE STABILIZATION OF ION TRAP RADIOFREQUENCY POTENTIALS

Disclosed are improved methods and structures for actively stabilizing the oscillation frequency of a trapped ion by noninvasively sampling and rectifying the high voltage RF potential at circuit locations between a step-up transformer and a vacuum feedthrough leading to the ion trap electrodes. We use this sampled/rectified signal in a feedback loop to regulate the RF input amplitude to the circuit. By employing techniques and structures according to the present disclosure we are advantageously able to stabilize a 1 MHz trapped ion oscillation frequency to




ac

Methods and Apparatus for Backside Integrated Circuit High Frequency Signal Radiation, Reception and Interconnects

In an example arrangement an apparatus includes a semiconductor substrate having a front side surface including circuitry and a backside surface opposing the front side surface; a plurality of metal conductors formed over a front side surface of the semiconductor substrate; at least one cavity opening etched in a backside surface of the semiconductor substrate; and a radiator formed in a portion of the metal conductors and configured to radiate signals through the cavity opening in the backside surface. Methods and additional apparatus arrangements are also disclosed.




ac

Method for controlling and optimizing the manufacture of gasoline blendstocks for blending with an alcohol as an oxygenate

A method for manufacturing an oxygenated gasoline-blend by blending a hydrocarbon Basestock for Oxygenate Blending (BOB) with an alcohol such as ethanol to a required octane specification first blends the BOB to an octane number, (RON+MON)/2 based on the octane sensitivity (RON−MON) of the BOB and the proportion of alcohol to be added to the BOB, such that when the BOB is blended with the specification proportion of alcohol to form the oxygenated gasoline blend, this blend will have the required octane specification. The blending of the BOB with the alcohol will typically be done at a location remote from that where the BOB is blended, e.g. at the product distribution terminal after being transported from the refinery by pipeline or tank car.




ac

Reusable heater in a package

A heater in a housing with an opening. The heater reacts with oxygen to produce heat and upon consumption of the oxygen, the heating reaction stops and can be restarted at a later point in time upon the introduction of additional oxygen and is used in a package for heating pre-moistened substrates such as sanitary wipes, and the like.




ac

Process for upgrading low rank carbonaceous material

A process for upgrading brown coal having a first water content including subjecting the brown coal to a conditioning step which includes heating the brown coal to a first temperature to produce a conditioned brown coal having a second water content which is lower than the first water content; attritioning the conditioned brown coal to enable water to be released from the microstructure of the brown coal and thereby producing an admixture of the brown coal and released water; forming aggregates of the admixture; drying the aggregates to produce upgraded brown coal having a third water content which is lower than the second water content.




ac

Two-stage reactor and process for conversion of solid biomass material

A two-stage reactor is disclosed for the conversion of solid particulate biomass material. The reactor is designed to maximize conversion of the solid biomass material, while limiting excess cracking of primary reaction products. The two-stage reactor comprises a first stage reactor, in which solid biomass material is thermally pyrolyzed to primary reaction products. The primary reaction products are catalytically converted in a second stage reactor.




ac

Conversion of triacylglycerides-containing oils

A process for converting triacylglycerides-containing oils into crude oil precursors and/or distillate hydrocarbon fuels is disclosed. The process may include reacting a triacylglycerides-containing oil-carbon dioxide mixture at a temperature in the range from about 250° C. to about 525° C. and a pressure greater than about 75 bar to convert at least a portion of the triacylglycerides to a hydrocarbon or mixture of hydrocarbons comprising one or more of isoolefins, isoparaffins, cycloolefins, cycloparaffins, and aromatics.




ac

Use of a reaction product of carboxylic acids with aliphatic polyamines for improving or boosting the separation of water from fuel oils

Use of a reaction product of saturated or unsaturated aliphatic mono- or polycarboxylic acids with aliphatic polyamines for improving or boosting the separation of water from fuel oils which comprise additives with detergent action. A Fuel additive concentrate comprising the said reaction product, certain additives with detergent action and optionally dehazers, cetane number improvers and solvents or diluents.




ac

METHOD OF MANUFACTURING REFINED PITCH

A method of manufacturing a refined pitch includes the steps of providing a pitch and performing a heated blending process thereon to produce a pitch solution; adding an aromatic additive to the pitch solution; adding an aliphatic additive to the pitch solution; performing a quiescent sedimentation process on the pitch solution; and separating a liquid part from the pitch solution. Therefore, the method allows a concentrated mesophase pitch to be manufactured quickly and by heat processing.




ac

DEVICES AND METHODS FOR SAMPLE CHARACTERIZATION

Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps.




ac

PACKING SYSTEM AND METHOD FOR CHROMATOGRAPHY COLUMNS

The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column;pre-sterilize a chromatography medium;introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.




ac

STACKABLE PLANAR ADSORPTIVE DEVICES

A lattice and distribution network for a stackable chromatography cassette comprising: a peripheral seal;at least one screen forming the lattice surrounded by the peripheral seal, each at least one screen comprising a plurality of struts in a latticed arrangement;a first internal distribution network fluidly coupled to the lattice and surrounded by the peripheral seal;a second internal distribution network disposed opposite the first internal distribution network, fluidly coupled to the lattice and surrounded by the peripheral seal;wherein a direction of fluid flow is established from the first internal distribution network through the lattice to the second internal distribution network; andwherein preferential streamlines are minimized.




ac

WATER TREATMENT METHOD AND WATER TREATMENT APPARATUS EACH USING MEMBRANE

Ozonated washing water is generated by injecting an ozone gas into pressurized washing water that is filtered water obtained by membrane filtration of untreated water and that is to be used at the time of backwashing, and the ozonated washing water is supplied to a membrane from the filtration secondary side to thereby remove a fouling substance inside the membrane, while causing ozone-containing bubbles to emerge in the filtration primary side to thereby remove a fouling substance on a membrane surface in the filtration primary side.




ac

Slideshow: Backbeat Psychosis at Burger Boogaloo 2015


John Waters, the venerable sultan of sleaze who we recently visited at home in San Francisco, presided over this year's Burger Boogaloo festivities in Oakland's Mosswood Park. He was the consummate host.…



  • Blogs/Arts & Music

ac

Bay Area Activists Protest Peter Thiel's Partnership With Immigration and Customs Enforcement

'Don’t build technology for Mordor.' On Saturday at Peter Thiel’s San Francisco home overlooking the Bay, protesters demonstrated against a collaboration between Immigration and Customs Enforcement and Palantir Technologies, a data-mining firm that Thiel co-founded. As reported in The Intercept, ICE will be using a new Palantir product called Investigative Case Management to to aid in its expanded deportation mission.…



  • News & Opinion/News

ac

Photos from the ANSWER and Indivisible Counter-Protests Against ACT for America's 'March Against Sharia'

ACT for America, which the Southern Poverty Law Center has designated as an anti-muslim hate group, showed up on Saturday morning in cities nationwide, dubbing its protest a "March Against Sharia." Berkeley was spared, but the group appeared in nearby Santa Clara, and in Roseville, a suburb of Sacramento.…




ac

Review: J Balvin Proved Why He's One of the World's Biggest Latinx Acts at Oracle Arena


The last time J Balvin was at Oracle arena was back on Feb. 15, 2015 when he was the opening act for Pitbull and Enrique Iglesias' joint tour. I was at that show with my mom as my date, and vividly remember her saying, “one day he’s going to headline his own tour here.” I brushed off her comment, it wasn’t that I didn’t believe that he was talented.…



  • Blogs/Arts & Music

ac

War and Peace

Beanpole sees post-WWII Russia through the eyes of two emotionally drained women. The scene is the former Leningrad in 1945, a few weeks after the close of World War II. The characters in Kantemir Balagov’s Beanpole are what’s left after the horrendous two-and-a-half-year siege of the city by the German invaders, in which some 800,000 civilians perished from cold and hunger.…




ac

Tacos Super Monilla brings over 50 years of taco experience to Bladium.

This Mexico City taquero serves tacos and tortas sure to satisfy soccer stars. Most gym-goers have a favorite post-workout meal. Some swear by chocolate milk with its blend of sugar, protein, and fat, while others swear by cottage cheese, oatmeal, or smoothies.…




ac

Evidence Suggests That Sheltering-In-Place is Working

Scroll from the bottom up to read in chronological order.…




ac

METHODS AND COMPOSITIONS FOR PREDICTING TOBACCO USE

Provided herein are methods of reliably determining whether or not an individual is a user of tobacco.




ac

VIRUS CAUSING RESPIRATORY TRACT ILLNESS IN SUSCEPTIBLE MAMMALS

The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single-stranded RNA virus (MPV) within the subfamily Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus Metapneumovirus and components thereof.




ac

MICROTENTACLE IMAGING IN PATIENT TUMOR SAMPLES

The present invention provides a method for imaging microtentacles on isolated, living, non-adherent primary tumor cells from a cancer subject comprising: i) obtaining one or more living, non-adherent primary tumor cells that has been isolated from a solid tumor from the subject; and ii) imaging the one or more living, non-adherent primary tumor cells and detecting the microtentacles.




ac

DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR

Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.




ac

SOLUBLE AND IMMUNOREACTIVE VARIANTS OF HTLV CAPSID ANTIGEN P24

The invention concerns soluble and antigenic HTLV p24 variants that can be fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising either the N- or the C-terminal domain of p24 and lacking the other domain. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focuses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.




ac

PATHWAY CHARACTERIZATION OF CELLS

The present invention provides methods, compositions and kits for the characterization of cellular pathways in cells containing genetic alterations.




ac

MATERIALS AND METHODS FOR DIAGNOSIS, PROGNOSIS AND ASSESSMENT OF THERAPEUTIC/PROPHYLACTIC TREATMENT OF PROSTATE CANCER

A method to detect prostate cancer comprising contacting a sample of prostate cells from the patient with a set of detectably labeled probes under hybridization conditions and determining the presence of chromosomal abnormalities in prostate tumor tissue, PIN (intra-epithelial neoplasia), histologically benign tissue and benign prostatic hyperplasia (BPH); a method to combine immunofluorescence and FISH (IF-FISH) to facilitate the assessment of chromosomal abnormalities; a set of probes; and a kit comprising the set of probes and instructions for diagnosing prostate cancer in a patient.