The implementation of the Multi-Year Tariff Order (MYTO) framework for electricity pricing has again been delayed for the fourth minor review which occurs every six months. Daily Trust reports that the tariff which should now be at an average of N51 per kilowatt hour (kWh) is still at N31.8 as the implementation of the reviews […]

NERC delays electricity tariff review for fourth time

The Managing Director of Abuja Electricity Distribution Company (AEDC), Ernest Mupwaya said his company has installed 88,000 meters and would install 120,000 units by December 2017 to tackle complaints on estimated billing. upwaya said this at the opening of a two day workshop on energy theft for judges within the Federal Capital Territory (FCT). He […]

Abuja DisCo meters 88,000 customers, targets 120,000 by December

Fossil fuel demand will reduce by nearly 25 percent in the next two decades, the Secretary General of the Organisation of Petroleum Exporting Countries (OPEC), Mohammed Sanusi Barkindo has said. Speaking at the SPE Kuwait Oil & Gas Show and Conference in Kuwait City, yesterday, Barkindo however said fossil fuels will remain a dominant in […]

Fossil fuel demand to by 25% by 2040 – OPEC

The Securities and Exchange Commission has ordered the Nigerian Stock Exchange to suspend trading on the shares of Oando Plc. The commission said in a circular yesterday it had carried out a comprehensive review of the petitions by Alhaji Dahiru Barau Mangal and Ansbury Incorporated and made the following findings amongst others; Breach of the […]

SEC suspends Oando shares from stock market

The House of Representatives yesterday waded into the ongoing controversy between the Nigeria Ports Authority (NPA) and Intels Nigeria Limited. The House, while adopting a motion by Rep Diri Douye (PDP, Bayelsa), resolved to constitute an ad hoc panel to probe the circumstances that led to NPA’s decision to terminate an existing contract between it […]

Reps ask NPA to reverse termination of agreement with Intels

To drive the enforcement of the compulsory insurance of public buildings across the country, The National Insurance Commission (NAICOM) in Abuja has inaugurated a technical committee. The committee was inaugurated by NAICOM’s Commissioner for Insurance, Mr Mohammed Kari, and it has membership drawn from NAICOM, the Federal Fire Service (FFS) from all the zones and […]

NAICOM moves to enforce compulsory insurance of public buildings

Over 1000 companies expressed interest in 17 categories of projects for the 2017 capital projects of the Nigerian Railway Corporation (NRC). The Managing Director of the NRC, Engr. Fidet Okhiria disclosed this on Tuesday while declaring open the process for the opening of bids for the projects. The formal opening of bids held at the […]

Over 1,000 firms bid for 2017 railway projects

Leading financial services provider, First City Monument Bank (FCMB), has been awarded the prestigious International Organisation for Standardisation (ISO) 9001:2015 certificate for Quality Management System. The Bank was presented with the certificate by the Standards Organisation of Nigeria (SON), following a comprehensive audit and evaluation exercise conducted by the Organisation. The exercise covered departments and […]

FCMB attains ISO Certification for Quality Management

On June 25, 2018 I woke up to yet another social media trend – a news story with the bold and quite salacious headline “How Safe Are Customer Deposits At FCMB?” had taken over the digital airwaves. Between the shares and likes and comments, a storm in a cup had brewed to great proportions in […]

The Worrisome Trend of Sensational Social Media “Journalism” and the Impact On Legitimate Business Concerns: Recent Travails of FCMB, GTBank and First Bank

FCMB Bank (UK) Limited, an independently incorporated subsidiary of First City Monument Bank Limited (which is a member of FCMB Group Plc), has introduced its Personal and Business banking proposition in London, United Kingdom and Lagos, Nigeria at an impressive ceremony attended by the top echelon of the business community within and from outside the […]

FCMB Bank (UK) Limited Launches Personal and Business Banking Proposition to Deepen Inclusiveness

Food waste has been a pressing concern for environmentalists and humanitarians for decades. The Food and Agriculture Organization (FAO) estimates that approximately one third of all global food products end up in landfills, equating to roughly 1.3 billion metric tons. Conversely, according to the Asia-Pacific Economic Cooperation (APEC), about 3 billion people lack access to […]

Bronisław Malinowski (1884 – 1942) did for several years conduct socio-anthropological research in the Trobriand Islands. Returning to England after World War I, he wrote several ground breaking books, among them Magic, Science, and Religion in which he assumed that people’s feelings and motives are crucial for understanding the way their society functions. Malinowski considered […]

"We’re playing with fire, but there can be no more playing for time. We’re out of time," says UN Secretary General António Guterres

At the 2021 UN Climate Summit, Barbados prime minister Mia Mottley called for more and better use of special drawing rights (SDRs), the International Monetary Fund’s reserve asset. The special drawing right is an international reserve asset created by the IMF. It is not a currency—its value is based on a basket of five currencies, […]

Critical levels of nationwide hunger in Sudan has only increased to critical levels since the start of the Sudanese civil war in April 2023. Escalated hostilities between the Sudanese Armed Forces (SAF) and the Rapid Support Forces (RSF) have led to limited mobility and repeated blockages of humanitarian aid. This, coupled with the volatile floods […]

The production of solar energy by means of panels installed on small farmers’ properties or on the roofs of community organisations is starting to directly benefit more and more farmers in Chile. This energy enables technified irrigation systems, pumping water and lowering farmers’ bills by supporting their business. It also enables farmers’ cooperatives to share […]

Affibody molecules are small (6-kDa) affinity proteins generated by directed evolution for specific binding to various target molecules. The first step in this workflow involves the generation of an affibody library, which can then be used for biopanning using multiple display methods. This protocol describes selection from affibody libraries using display on Staphylococcus carnosus. Display of affibodies on staphylococci is very efficient and straightforward because of the single cell membrane and the use of a construct with a constitutive promoter. The workflow involves display of affibody libraries on the surface of S. carnosus cells, followed by screening and selection of binders using fluorescence-activated cell sorting (FACS). The transformation of DNA libraries into S. carnosus is less efficient and more complicated than for Escherichia coli. Because of this, staphylococcal display is suitable for affinity maturation or other protein-engineering efforts that are not dependent on very high diversity, and thus magnetic-activated cell sorting (MACS) is often not required before FACS. However, MACS is an option, and MACS procedures used for E. coli can easily be adapted for use in S. carnosus if needed.

Affibody molecules are small (6-kDa) affinity proteins generated by directed evolution for specific binding to various target molecules. The first step in this workflow involves the generation of an affibody library, which can then be used for selection via multiple display methods. This protocol describes selection from affibody libraries by Escherichia coli cell surface display. With this method, high-diversity libraries of 10¹¹ can be displayed on the cell surface. The method involves two steps for selection of binders from high-diversity libraries: magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). MACS is used first to enrich the library in target-binding clones and to decrease diversity to a size that can be effectively screened and sorted in the flow cytometer in a reasonable time (typically <10⁷ cells). The protocol is based on methodology using an AIDA-I autotransporter for display on the outer membrane, but the general procedures can also be adjusted and used for other types of autotransporters or alternative E. coli display methods.

Affibody molecules are small (6-kDa) affinity proteins generated by directed evolution for specific binding to various target molecules. The first step in this workflow involves the generation of an affibody library. This is then followed by amplification of the library, which can then be used for biopanning using multiple methods. This protocol describes amplification of affibody libraries, followed by biopanning using phage display and analysis of the selection output. The general procedure is mainly for selection of first-generation affibody molecules from large naive (unbiased) libraries, typically yielding affibody hits with affinities in the low nanomolar range. For selection from affinity maturation libraries with the aim of isolating variants of even higher affinities, the procedure is similar, but parameters such as target concentration and washing are adjusted to achieve the proper stringency.

Affibody molecules are small (6-kDa) affinity proteins folded in a three-helical bundle and generated by directed evolution for specific binding to various target molecules. The most advanced affibody molecules are currently tested in the clinic, and data from more than 300 subjects show excellent activity and safety profiles. The generation of affibody molecules against a particular target starts with the generation of an affibody library, which can then be used for panning using multiple methods and selection systems. This protocol describes the molecular cloning of DNA-encoded affibody libraries to a display vector of choice, for either phage, Escherichia coli, or Staphylococcus carnosus display. The DNA library can come from different sources, such as error-prone polymerase chain reaction (PCR), molecular shuffling of mutations from previous selections, or, more commonly, from DNA synthesis using various methods. Restriction enzyme-based subcloning is the most common strategy for affibody libraries of higher diversity (e.g., >10⁷ variants) and is described here.

Sleep is a fundamental feature of life for virtually all multicellular animals, but many questions remain about how sleep is regulated and what biological functions it plays. Substantial headway has been made in the study of both circadian rhythms and sleep in the fruit fly Drosophila melanogaster, much of it through studies of individual fly activity using beam break counts from Drosophila activity monitors (DAMs). The number of laboratories worldwide studying sleep in Drosophila has grown from only a few 20 years ago to hundreds today. The utility of these studies is limited by the quality of the metrics that can be extracted from the data. Many software options exist to help analyze DAM data; however, these are often expensive or have significant limitations. Therefore, we describe here a method for analyzing DAM-based data using the sleep and circadian analysis MATLAB program (SCAMP). This user-friendly software has an advantage of combining several analyses of both sleep and circadian rhythms in one package and produces graphical outputs as well as spreadsheets of the outputs for further statistical analysis. The version of SCAMP described here is also the first published software package that can analyze data from multibeam DAM5Ms, enabling determination of positional preference over time.