When the World Curling Federation cancelled the women's, men's and mixed doubles championships set for March and April, it said it would be discussing potential options including rescheduling the events — perhaps playing them in the fall at one venue. CBC Sports has learned that will no longer be the case.

The World Curling Federation announced Thursday it is proposing to the International Olympic Committee that the 2021 world curling championships will determine which countries will book their tickets to Beijing 2022.

Colleen Jones, Sidney Crosby among many preeminent local sports figures reacting in the aftermath of Sunday's killing rampage.

Will he, given his obsession with the economy?

The notion that he is bound for four more years is pure superstition.

Is the Republican indulgence of the president bottomless?

Join our Facebook live on Thursday at noon with Dr. Roselyn Wilson a psychiatrist at St. Joseph’s Healthcare Hamilton’s Youth Wellness Centre. She'll talk with us about how COVID-19 is heightening anxiety in some young people. We'll talk about how to help the young people in your life through the pandemic.

Front-line nurses from the West 5th campus use a special van to meet patients with mental health problems — primarily those living with schizophrenia— and treat them by doing their blood work.

A Toronto law firm has filed to launch a class action lawsuit against a Niagara Falls long-term care home where 18 residents have died from COVID-19, saying the home had a pub night in the midst of the pandemic.

Sofina Foods plants in Burlington and Mississauga have each had an employee test positive.

A search warrant executed by Hamilton police vice and drugs officers at an address in the city’s east end has resulted in the arrest of a couple and multiple charges, police said in a Friday release.

Dr. Roselyn Wilson, a psychiatrist at St. Joseph’s Healthcare Hamilton’s Youth Wellness Centre, offers some tips to help young people cope with the stress and anxiety they face while on lockdown under COVID-19.

Hamilton Conservation Authority is re-opening the Hamilton-to-Branford Rail Trail, while the city announced the reopening of the Hamilton Waterfront Trail, between Confederation Park and the Burlington Lift Bridge.

Normal life is slowly resuming as the city of Hamilton begins to relax tight measures set in place because of COVID-19. Mayor Fred Eisenberger said now it's up to the public to help the city move in the right direction by continuing to stay two metres apart from each other and be cautious.

McMaster University is planning for fewer international students, more deferred acceptance offers and online teaching for the thousands who are attending as the start of a new school year looms in the near future and in the middle of the COVID-19 pandemic.

This introduction describes the features and uses of ethidium bromide, methylene blue, and SYBR dyes for staining nucleic acids.

Quantifying RNA is an important and necessary step before most RNA analysis techniques. Methods for quantifying RNA can be classified into two categories: ultraviolet (UV) spectrophotometric methods, which are based on the absorption spectra of the purine and pyrimidine bases; and fluorescent dye-based methods, which measure the fluorescence intensity of dyes that selectively fluoresce when bound to nucleic acids. If the RNA sample is pure (i.e., without significant amounts of contaminants such as proteins, phenol, agarose, or other nucleic acids), UV spectrophotometric measurement of the amount of UV irradiation absorbed by the bases is simple and accurate. However, if the sample contains significant quantities of impurities or if the concentration of RNA is very low, it is better to use fluorescent dye-based methods. An overview of spectrophotometric and fluorescent dye-based RNA quantification methods is given here, as are several options for storing purified RNA preparations. Proper storage of RNA samples is important; it can help minimize RNase contamination and consequent sample degradation.

Determining the concentration of protein samples generally is accomplished either by measuring the UV absorbance at 280 nm or by reacting the protein quantitatively with dyes and/or metal ions (Bradford, Lowry, or BCA assays). For purified proteins, UV absorbance remains the most popular method because it is fast, convenient, and reproducible; it does not consume the protein; and it requires no additional reagents, standards, or incubations. No method of protein concentration determination is perfect because each is subject to a different set of constraints such as interference of buffer components and contaminating proteins in direct UV determination (A₂₈₀) or reactivity of individual proteins and buffer components with the detecting reagents in colorimetric assays. In cases in which protein concentration is critical (e.g., determination of catalytic rate constants for an enzyme), it may be advisable to compare the results of several assays.

The Xenopus oocyte is a unique model system, allowing both the study of complex biological processes within a cellular context through expression of exogenous mRNAs and proteins, and the study of the cell, molecular, and developmental biology of the oocyte itself. During oogenesis, Xenopus oocytes grow dramatically in size, with a mature oocyte having a diameter of ~1.3 mm, and become highly polarized, localizing many mRNAs and proteins. Thus, the mature oocyte is a repository of maternal mRNAs and proteins that will direct early embryogenesis prior to zygotic genome transcription. Importantly, the Xenopus oocyte also has the capacity to translate exogenous microinjected RNAs, which has enabled breakthroughs in a wide range of areas including cell biology, developmental biology, molecular biology, and physiology. This introduction outlines how Xenopus oocytes can be used to study a variety of important biological questions.

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