The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.

Monoclonal antibodies (mAbs) having thyroid stimulating activity (TSAb), especially full or considerably agonistic activity, or thyroid blocking activity (TBAb), which are obtainable by genetic immunization of mice, or fragments (F(ab')2, Fab or Fv) or humanized forms of such monoclonal antibodies or single chain forms (SCA; scFv) of such fragments, which antibodies, or their fragments, compete with bovine TSH for epitopes of the human TSHr, compete with autoantibodies from sera from Graves' patients as well as with autoantibodies from sera from patients harboring blocking autoantibodies for epitopes of the human TSHr, bind to conformational epitopes of the human TSHr located in the first 281 amino acids of the human TSHr, and usually also bind to TSFR receptors (TSHr) from different animals. Various uses of such antibodies, or of peptides corresponding to variable regions of such antibodies, are also described and claimed.

Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the device further comprises filtering means for removing precipitated protein particles. The filtering means is a size exclusion filter or a polymeric or inorganic monolith having a maximum pore size less than or equal to the diameter of the particles to be removed from the sample, and can be integral with the sorbent or associated with the sorbent. The sorbent is characterized by sufficient selectivity between the matrix interfering agents and analytes of interest to provide retention of the matrix interfering agents while providing elution of the analytes of interest (e.g., a reversed phase or a polar modified reversed phase). Typical devices incorporating these features include luer syringe filters, individual filter cartridges, multiwell plates, pipette tips, or inline columns for multiple or single use.

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.

Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes an LNT1 polypeptide.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The invention relates to genes coding for TCP family transcription factors and having a biological role in the development of axillary buds and branch growth. Furthermore, the invention relates to the promoters of the transcription of said genes, to the genetic constructs containing same and to the uses thereof, including the use of agents that modulate the expression of these genes in order to modify plant architecture.

A novel maize variety designated PH1TWW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1TWW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1TWW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1TWW or a locus conversion of PH1TWW with another maize variety.

A novel maize variety designated PH1M9D and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1M9D with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1M9D through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1M9D or a locus conversion of PH1M9D with another maize variety.

The present invention relates to a composition useful for the diagnosis of diseases associated with aberrant expression of the genes encoding the secreted proteins Futrin 1, 2, 3 and/or 4(=R-Spondin 2, 3, 1 and 4, respectively), e.g. in connection with tumors or diseases of the muscle, kidneys or bones. The present invention also relates to a pharmaceutical composition containing a compound which is capable of modifying (a) the expression of the gene encoding Futrin 1, 2, 3 and/or 4 or (b) the activity of the Futrin 1, 2, 3 and/or 4 protein.

The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.

An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

Disclosed are process for contacting a protein containing material with one or more wet-mill streams. The protein content of the protein containing material is increased.

The present invention relates, in general, to gene expression and, in particular, to a method of inhibiting the expression of a target gene and to constructs suitable for use in such a method.

A vehicle seat can include a seat bottom frame having a front, a back opposite the front, and enclosing a volume. Pivotally connected to the back of the seat bottom frame can be a seat back frame and pivotally connected to the front of the seat bottom frame can be a seat leg rest. The seat leg rest has a length. Mounted in the volume of the seat frame are at least two leg rest springs which are connected to the leg rest. When the leg rest springs are actuated, the seat leg rest can be directly pivoted approximately and incrementally parallel with the seat bottom frame. The two leg rest springs are mounted a first distance apart and the first distance is less than half of the length of the leg rest.

A stylish ready-to-assemble chaise lounge and sofa which may be easily and quickly assembled by a consumer, is provided. The invention is a kit from which either a stylish chaise lounge or a sofa may be easily assembled by a consumer using a single Allen wrench. The kits, both chaise and sofa, comprise a seat base, a long side seatback and a short side seatback, feet, fasteners and cushions. With regard to the chaise, the long and short side chaise seatbacks have rectangular lower portions with upper portions featuring a flowing curved “waterfall” seating surface which improves the comfort of a user leaning back against the seatback.

A premium class passenger suite that includes a main seat positioned in the suite together with separate bed. The bed has a flexible mattress of predetermined dimensions and is movable between a stowed position to one side of the main seat and a deployed position above and separate from the main seat. A drive apparatus is provided for driving the bed between the stowed and deployed positions. The main seat is configured to be movable between a seating position when the bed is stowed, and a stowed position with a lowered seat back when the bed is deployed for use.

A seating unit that includes a linkage mechanism adapted to move the seating unit between seat-lift, closed, extended, reclined, and seat-lift positions is provided. The linkage mechanism includes a seat-mounting plate mounted to a footrest assembly, a base plate fixedly mounted to a lift assembly, a back-mounting link rotatably coupled to the seat-mounting plate, a seat-adjustment assembly with a bellcrank, and a linear actuator for automating adjustment of the linkage mechanism. In operation, a stroke in a first phase of the linear actuator generates a force on the bellcrank that translates the seat-mounting plate rearward in a consistent angle of inclination and rotates the back-mounting link from a reclined to an upright orientation. A stroke in a second phase acts to collapse the footrest assembly. A stroke in a third phase causes the lift assembly to raise and tilt the seating unit, thereby accommodating egress and ingress of an occupant.

A wire harness for a vehicle seating assembly includes a body portion having a plurality of engagement hooks adapted for engagement with a vehicle seat cushion pan. A latticework of interconnected lateral support members are bound by a peripheral frame.

More specifically, the crash indication system for a child safety seat includes a seat bottom. A base is provided for supporting the seat bottom, which is slidably connected to the bases so that the seat body is movable relative to the base, between a resting position and a ride down position, a position due to a change in environmental forces. The seat body is adjustably affixed to a carriage to set a recline position. An indicator member is attached to the carriage and moves therewith to visually track and monitor the movement of the seat. When ride down of the seat occurs due to a change in forces to the seat, the indicator member moves with the sliding carriage to reflect a ride down position of the seat bottom. A ratchet connection is provided to prevent the carriage from going back to its resting position.

A cushion element for a vehicle seat or headrest comprises a non-woven layer and a fiber composite material. An insert material is arranged between the non-woven layer and the fiber composite material, and has different compression properties than the fiber composite material. The fiber composite material forms together with the insert material and the non-woven layer a one-piece material composite.

A resistance having a high impedance is connected between anode terminals of two variable capacitance diodes sharing a cathode, and the components described above are sealed in one package. The resistance can be formed of a diffusion region between p-regions of the variable capacitance diodes or can be formed of polysilicon and disposed on a chip. Thus, the resistance can be mounted while a chip size of the variable capacitance diode is maintained. Accordingly, it is not required that a bias resistance having a high impedance is additionally provided, whereby achieving reduction in the substrate mounting area and reduction in costs of the set.

The present invention provides an apparatus for enhancing Q factor of an inductor. The apparatus includes a negative resistance generator coupled to the inductor for providing a negative resistance, and a bias circuit coupled to the negative resistance generator for biasing the negative resistance generator.

Provided is an oscillator including: a MEMS resonator for mechanically vibrating; an output oscillator circuit for oscillating at a resonance frequency of the MEMS resonator to output an oscillation signal; and a MEMS capacitor for changing a capacitance thereof caused by a change in a distance between an anode electrode and a cathode beam according to an environmental temperature.

The invention discloses a varactor device (100) for improved temperature stability, comprising a first varactor (160) connected to a decoupling network (150). The device further comprises a voltage stabilizer (110), said stabilizer comprising a capacitor (140) and a temperature dependent capacitor (130), and in that the stabilizer comprises means for connection to a DC-feed (120). Suitably, the decoupling network (150) is connected in parallel to the first varactor (160), and the capacitor (140) of the voltage stabilizer (110) is connected in parallel to the decoupling network (150), the temperature dependent capacitor (130) of the voltage stabilizer (110) being connected in series to the diode of the voltage stabilizer (110).

There are provided a single package television tuning apparatus and a television receiver including the apparatus. The apparatus includes: a printed circuit board; a switching part installed on the printed circuit board and selecting one of a first radio frequency signal and a second radio frequency signal inputted; a tuner part installed on the printed circuit board and down-converting one of the first radio frequency signal and the second radio frequency signal, the one selected by the switching part; first and second input connectors receiving the first radio frequency signal and the second radio frequency signal; and a chassis supporting the first and second input connectors, covering the printed circuit board, the switching part, and the tuner part, and electrically connected to grounds of the switching part and the tuner part.

The present invention provides a central frequency adjustment device and adjustable inductor layout; wherein, the central frequency adjustment device is applied in an inductor/capacitor tank (LC tank) for adjusting the central frequency of the LC tank. The device comprises a first inductor with a first end and a second end; a second inductor with one end coupled with the second end of the first inductor; and, a first trimmable wire connected to the first inductor in parallel and to the second inductor in series, which adjusts the central frequency by cutting off the first trimmable wire.

A broadcast receiving device includes a card slot, a fan, a temperature sensor, a memory component and a control unit. The card slot accepts an IC card. The fan rotates to cool the IC card. The temperature sensor measures a first temperature. The memory component stores correlation information indicating a correlation between the first temperature and a second temperature of the IC card. The control unit acquires the second temperature based on the first temperature and the correlation information. The control unit determines if the second temperature exceeds a predetermined temperature. The control unit switches from a first output mode, in which an audio-video signal is outputted via the IC card, to a second output mode, in which the audio-video signal is outputted by bypassing the IC card, when the control unit determines that the second temperature exceeds the predetermined temperature.

A communication module is provided in which its characteristic of separation between its first and second tuner units is improved. The module is equipped with a circuit board having a first main surface, and a second main surface opposite to the first main surface; a first amplifier arranged on the first main surface, for amplifying a first signal; a first mixer arranged on the first main surface, for converting a signal supplied from the first amplifier to an intermediate-frequency signal; a second amplifier for amplifying a second signal; and a mixer for converting a signal supplied from the second amplifier to an intermediate-frequency signal, both arranged on the second main surface of the circuit board.

A receiver apparatus 3 has a digital circuit portion 13 that converts a compressed digital signal outputted from a digital demodulating portion 12 into digital video and audio signals and a video/audio output circuit 14 that converts the digital video and audio signals outputted from the digital circuit portion 13 into analog video and audio signals. With this configuration, a video/audio processing IC for digital signal processing no longer needs to be mounted on the circuit board of a video display apparatus 4, and thus a receiver system can be realized with a video display apparatus having a simple configuration.

A band-pass filter device includes: a plurality of band-pass filter elements on a principal plane of a substrate; wherein the band-pass filter elements correspond to a plurality of respective channels divided by frequency regions, and each have a plurality of piezoelectric resonators. Each of the piezoelectric resonators includes a piezoelectric film whose periphery is supported by the substrate, a first electrode formed on a lower surface of the piezoelectric film, a second electrode formed on an upper surface of the piezoelectric film and formed in a state of overlapping at least a part of the first electrode with the piezoelectric film interposed between the second electrode and the first electrode, a lower space formed between the substrate and the piezoelectric film, and an upper space formed over the piezoelectric film.

Aspects and embodiments of the present invention provide a system for obtaining, processing and managing data from an implanted sensor. In some embodiments, a patient or other persons can use a flexible antenna to obtain data from the implanted sensor. The flexible antenna includes at least one transmit loop and at least one receive loop. The transmit loop is adapted to propagate energizing signals to the implanted sensor. The receive loop is adapted to detect a response signal from the implanted sensor. The transmit loop includes a capacitor formed by a discontinuous area. The capacitor is adapted to allow the loop to be tuned. The flexible antenna can communicate with a patient device that collects the data from the implanted sensor, creates a data file and transmits the data file to a remote server over a network. A physician or other authorized person may access the remote server using an access device.

A tuner and a transformer formed by printed circuit board thereof are provided. The transformer includes a first winding and a second winding. In which, the first winding forms a first inductor and the second winding forms a second inductor. The transformer is formed by the first and the second inductors, wherein the first winding and the second winding are formed by conducting wires of a printed circuit board.

Remote programmable reference systems and methods are described. A remote programmable reference system maintains a set of parameters from which a reference signal can be generated to transmit the reference signal to a system platform. In an embodiment, a parameters update can be received from which a modified reference signal can be generated to transmit the modified reference signal. In another embodiment, the remote programmable reference system maintains multiple sets of parameters from which respective reference signals can be generated for any one or more system platforms having different system configurations. The system platforms can interface with the remote programmable reference system through a system interface via a communication network.

An antenna assembly is operative for receiving interrogating radiation at a variable frequency tag and generating a corresponding received signal, and for receiving a signature signal and radiating corresponding response radiation. A logic unit is operative for receiving the received signal and outputting the signature signal in response, the signature signal including a signature code for use in identifying the tag. A voltage controlled oscillator is operative for controlling a rate at which the signature code is output; and a power supply is operative for providing an electrical potential difference for energizing the tag. The voltage controlled oscillator is operable to output the signature code at a rate which is governed by the magnitude of the received signal.

Methods and apparatus for computing the carrier frequency of a transmitter using frequency modulated digital data to compensate for frequency shifting of the transmitter and the receiver local oscillators and for bandwidth adjustment of the receiver's filter. In particular, methods and apparatus are disclosed for binary systems transmitting “1” and “0” data using decoded or undecoded received signals.

In an oscillating apparatus, a detection unit detects a frequency offset between an input signal and a reference signal. A code generation unit specifies a relationship among a code having a predetermined number of bits, the frequency offset, and a voltage to be applied to a voltage-controlled oscillator by a DAC, in accordance with a frequency offset detection state of the detection unit. The code generation unit also generates a frequency offset correction code having a predetermined number of bits in accordance with the specified relationship. The DAC applies the voltage to the voltage-controlled oscillator, in accordance with the relationship described above and the code generated by the code generation unit. The voltage controlled oscillator outputs an oscillator signal having an oscillation frequency corresponding to the voltage applied by the DAC.

A circuit (C1-C4) is employed in a TWACS transponder (T) installed in an electric meter (M). The transponder generates inbound signals (IB) transmitted from the location of the electric meter to a central location (R). Firmware (F) within the transponder controls the flow of current for each pulse through the circuit by triggering a semi-conductor device such as a SCR (X1) or TRIAC (X2). The resulting current flow through the inductor for a subsequent pulse, regardless of the pulse's polarity, will be in the opposite direction to that of the previous pulse. The result is to maintain a constant level of magnetization of the inductor core which does not have to be overcome by energy in the subsequent pulse resulting in amplitude of all the pulses imposed on an AC waveform being substantially the same.

An electrical component provides a ceramic element located on or in a dielectric substrate between and in contact with a pair of electrical conductors, wherein the ceramic element includes one or more metal oxides having fluctuations in metal-oxide compositional uniformity less than or equal to 1.5 mol % throughout the ceramic element. A method of fabricating an electrical component, provides or forming a ceramic element between and in contact with a pair of electrical conductors on a substrate including depositing a mixture of metalorganic precursors and causing simultaneous decomposition of the metal oxide precursors to form the ceramic element including one or more metal oxides.

A stopper structure includes a wall defining an opening. A rotary electronic component has a rotational shaft extending through the opening. A channel stopper has a tubular body and an engagement projection. The tubular body is detachably mounted onto the rotational shaft and is inhibited from rotating relative thereto. The engagement projection extends from an end of the tubular body in an axial direction and a radial direction with respect to the rotational shaft. A rotary operation member is detachably mounted onto the rotational shaft and the tubular body and is inhibited from rotating relative thereto. The rotary operation member has an axial hole in which at least portions of the rotational shaft and the tubular body fit. A limiting portion provided on the wall is engageable with the engagement projection for limiting a range of rotation of the rotational shaft.

Provided are a tuner module and a receiving apparatus capable of providing great degrees of variation and freedom to a layout in a main board, improving a degree of freedom of design, and achieving low cost. A tuner module includes a tuner module board on which a tuner functional unit is formed, a main board on which the tuner module board is surface-mounted, and a shield casing including at least one antenna connector, arranged to cover the surface-mounted tuner module board, and fixed to the main board. In the shield casing, a length of a longest long-side is a length equal to or less than ¼ of a wavelength of a highest received frequency in the air.

A tuner module includes a tuner module substrate having a tuner function part formed thereon, a case body having a shielding function of holding and incorporating at least the tuner module substrate, a circuit board on which the tuner module substrate is mounted, and at least one antenna connector to be fixed to the case body and having a core wire connected to the tuner module substrate incorporated in the case body. A part of the circuit board is contained inside the case body.

The invention provides an aqueous ink comprising a polymer having hydroxyl groups and a pigment, which can provide images excellent in scratch resistance and highlighter resistance and can inhibit the seeping out phenomenon of the polymer and the deterioration of storage stability and ejection characteristics even when it is stored for a long period of time. The aqueous ink comprises a polymer having hydroxyl groups and a pigment, wherein a proportion of a content of a polymer that is not adsorbed on the pigment out of the polymer having hydroxyl groups to the content of the pigment in the ink is 2.0 mass % or more, and a content (mass %) of the polymer that is not adsorbed on the pigment out of the polymer having hydroxyl groups is 0.20 mass % or less based on the total mass of the ink.

A recording apparatus that forms an image on a recording medium includes a U-turn conveying path and a redirecting conveying path. The U-turn conveying path reverses the front and back of the recording medium and conveys the recording medium to a conveying roller. The redirecting conveying path reverses a conveying direction of the recording medium, fed from the front side of a main body of the apparatus, at an upstream side of the conveying roller in the conveying direction, and conveys the recording medium to the conveying roller. Then, a first recording medium is conveyed along the U-turn conveying path, and a second recording medium is conveyed along the redirecting conveying path.

A pattern printing system and data processing method thereof are disclosed, which are suitable for printing patterns on printed circuit boards or data format rearrangement printing used in displays. The pattern printing method includes a process for interpreting scription data into matrix data, a procedure for modulating the print head resolution and the printing resolution, a procedure for interpreting and transmitting data commands, a procedure for rearranging memory data, and a procedure for firing data synchronously so as to achieve high-resolution printing and to continuously modulate any print data.

An inkjet recording apparatus to print onto a surface of a recording medium includes an endless rotation conveyance member with a plurality of through-holes; a suction unit to suction the recording medium on the surface of the endless rotation conveyance member; an ink discharge head including ink discharge nozzles; a blank discharge ink receiver to receive ink through the through-holes; a control unit to make the ink discharge nozzles discharge ink at a predetermined timing; and a fixed plate along an inner surface of the endless rotation conveyance member, the fixed plate including an opening formed in a position corresponding to the ink discharge nozzles and a through-hole. The blank discharge ink receiver is disposed corresponding to the opening of the fixed plate and in close contact with the fixed plate so that an inside space of the blank discharge receiver becomes a substantially closed space.

A toll violation enforcement system that contains a capture unit that receives a trigger signal and takes vehicle images of passing vehicles. A lighting unit configured to operate continuously is provided to establish a major lighting source incident on the vehicle. A processing unit is provided that processes the vehicle image and controls the settings employed by the capture unit. The processing unit preferably contains a plate location module for extracting an original resolution license plate area image from a first vehicle image; an exposure control module for controlling the exposure setting on the capture unit to provide consistent intensity of the license plate area; a resolution reset module for changing the image resolution for a second vehicle image; an image enhancement module for brightening pixels of the second vehicle image; and an image compression module for compressing the license plate area image and second vehicle image to a predetermined format.

A pen which is able to modulate the trace of the pen in response to a sensory signal of the user, such as for example skin conductance or respiration. The sensory signals represent the mood of the user. By changing the trace of the pen, a change in mood of the user can be expressed.

Currency bills are received, transported, and imaged to produce image data from which a visually readable image of each currency bill can be reproduced. Each of the currency bills includes a denomination, a serial number, and a set of secondary identifiers. One of the currency bills is determined to be a suspect bill. The suspect bill serial number is attempted to be extracted from the image data associated with the suspect bill. In response to failing to extract a complete serial number of the suspect bill, a serial number field in a suspect report is populated with a serial number snippet image.